• Title/Summary/Keyword: Hamster

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실험 동물용 사료

  • 홍성택
    • KOREAN POULTRY JOURNAL
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    • v.6 no.8 s.58
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    • pp.113-115
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    • 1974

  • 실험동물이 우리 인간과 관계가 깊은 것은 주지의 사실이다. Rat, Mouse, Hamster, Guinen-pig, 개, 원숭이들은 우리 인간의 의학의 발전뿐만 아니라 타동물과의 관계등을 연구하기 위해 실험용으로 지대한 공헌을 해왔으며 또 앞으로도 해나갈 것이다. 인간 자체를 실험용으로 쓸 수 없는 우리 인간은 이러한 실험동물을 육성하기 위한 연구도 활발히 진행하고 있다. 이에따라 실험동물의 사료개발도 다양하게 개발되고 있다. 어떤사료를 얼마나 어떤 상태로 급여해야만 할 것인가를 2회에 걸쳐 대한사료 홍성택씨가 기고해 주기로 했다.

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Characterization of Putrescine Uptake in Hamster Amelanocytic Melanoma AMEL-3 Cells

  • Garcia-Fernandez, Antonio J.;Rodriguez, Rosa A.;Perez-Pertejo, Yolanda;Balana-Fouce, Rafael
    • Molecules and Cells
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    • v.20 no.1
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    • pp.127-135
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    • 2005

  • The uptake of putrescine, spermidine and spermine by Fortner's hamster amelanocytic melanoma AMEL-3 cells was observed in this study to be time-dependent, temperature-sensitive, pH-dependent and saturable. Metabolic poisons nullified polyamine uptake, an indication that this is an energy-requiring mechanism. The presence of $Na^+$ ions was found to be requisite to full activity. Valinomycin, gramicidin, monensin and the calcium ionophore calcimycin were also observed to inhibit the process substantially. The transporter active site would seem to contain sulfhydryl groups. Other diamines and polyamine analogues, as well as cationic diamidines, suppressed putrescine uptake. The presence of the ornithine decarboxylase inhibitor DFMO in the culture medium induced putrescine inflows. Putrescine, in turn, induced the negative expression of the carrier, thus suggesting that this influx mechanism is governed by up/down regulation. The cationic diamidine CGP 40215A and its analogue CGP039937A competitively inhibited putrescine transport, with Ki values of 1.9 and $15{\mu}M$, respectively. The role of polyamine uptake in these cultures is discussed.

  • KSCI