• 한국해양생명과학회지
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• 제6권2호
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• pp.58-65
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• 2021

노화가 진행될수록 활성산소종으로 인하여 피부 보습은 떨어지고 피부 장벽은 붕괴되어 피부가 손상된다. 본 연구에서는 인천 동막 해변에 서식하는 염생식물인 갯끈풀(Spartina anglica; SAE)과 갯메꽃(Calystegia soldanella; CSE)을 70% 에탄올(EtOH)로 추출하여 피부 보습 및 피부 장벽 기능 강화에 대한 효능을 평가하였다. 이 추출물들에 대한 피부 각질형성세포(HaCaT cell)에서 세포독성을 WST-8 assay를 이용하여, 세포 생존율이 90% 이상을 보이는 농도를 선별하여 추가 실험을 진행하였다. ABTS 라디칼 소거능을 통해 항산화 효과를 확인한 결과, SAE와 CSE는 높은 라디칼 소거능을 보였다. 피부 보습과 관련된 인자들인 filaggrin (FGL), aquaporin 3(AQP3), hyaluronan synthase 2 (HAS2)과 피부 장벽 기능과 연관 있는 transglutaminase 1 (TGM1)과 involucrin (INV)의 유전자 수준에서의 발현 변화를 측정한 결과, SAE에 의해 AQP3, HAS2, TGM1의 발현이 증가하였으나, CSE는 변화가 없는 것을 확인할 수 있었다. SAE에 의한 세포 내 신호전달 경로를 확인하기 위해 western blot 분석을 수행하였다. Extracellular signal regulated kinase (ERK), c-Jun N-terminal kinase (JNK) and p38 mitogen activated protein kinase의 활성이 SAE에 의하여 상향 조절되었음을 확인하였다. 이러한 결과는 갯끈풀 추출물이 피부 보습 및 피부 장벽 기능 강화를 위한 화장품의 기능성 소재로 사용될 수 있음을 시사한다.

• 한국생물환경조절학회:학술대회논문집
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• 한국생물환경조절학회 2007년도 춘계 학술대회 및 정기총회
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• pp.257-260
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• 2007

• Journal of Ecology and Environment
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• 제32권2호
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• pp.115-121
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• 2009

In order to elucidate the mechanisms affecting plant distributions in the reclaimed dredging area in the Gwangyang steelworks, in the Gwangyang Bay, Korea, we examined soil characteristics and plant distributions in four study sites and a control site in the study area. Desalination occurring along a gradient with increasing elevation, resulting in decrease of soil pH, EC, P, K, Cl, Ca, Mg, and salt and an increase in soil T-N, silt, clay contents. From site 1 (the lowest-elevation site) to site 5 (the highest-elevation site), halophytes decreased in abundance and nonhalophytes increased. The dominant species in each site were: Phragmites communis, Limonium tetragonum, and 12 additional species at site 1, Carex pumila, Suaeda japonica, and 15 additional species at site 2, Spergularia marina, Scirpus planiculmis, and 22 additional species at site 3, Miscantus sinensis, Lespedeza bicolor, and 26 additional species at site 4 and Pinus thunberii, Rhododendron mucronulatum, and 39 additional species at site 5, which resembled a naturally-occurring P. thinbergii community. Cluster analysis of the vegetation data matrix grouped the 35 plots into 5 major groups, and cluster analysis using the soil environment data matrix revealed 4 major groups. CCA of the floristic and environmental data matrix showed a positive relationship of SAR, EC, Na, Cl, and Ca, which are related to salt, in the $1^{st}$ axis and $2^{nd}$ axis, but negative relationships for altitude, organic contents, silt, and clay contents. Notably, plant species in the reclaimed dredging area that were separated along the $1^{st}$ axis showed strong relationships with factors that related to salt. Long-term exposure to natural rainfall in the reclaimed dredging area changed the soil characteristics, such as salinity. This change in soil characteristics might alter the SAR, which affects plant survival strategies in a given habitat. These results strongly indicated that factors related to salt and elevation play important roles in determining the overall plant distribution in the reclaimed dredging area.

• 한국자원식물학회지
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• 제26권2호
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• pp.178-213
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• 2013

본 연구는 백령도의 관속식물을 밝히고 주요 식물들의 분포를 조사하였다. 2011년 8월부터 2012년 11월까지 총 5회에 걸쳐 수행되었다. 그 결과 관속식물은 124과 411속 647종 8아종 68변종 7품종 2교잡종으로 총 732분류군이 확인되었으며, 처음으로 밝혀진 분류군은 193분류군이었다. 이 중 환경부 지정 멸종위기야생식물은 2분류군, 한국 고유종 8분류군, 식물구계학적 특정식물 78분류군, 희귀식물 20분류군이 조사되었다. 또한 남 북방계식물 30분류군, 염생식물 43분류군이 발견되었다. 귀화식물은 92분류군으로 확인되었으며, 귀화율은 12.5%로 나타났다.

• 한국자원식물학회지
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• 제20권1호
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• pp.50-62
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• 2007

본 연구는 시화호 갈대습지공원내의 식물상 현황과 분포를 파악하여 본 지역의 식생생태계 관리 및 보전과 향후 지속적인 식생모니터링(Vegetation monitoring)을 위한 기초 자료로 활용하고자 수행되었다. 시화호 갈대습지공원내 반월천, 동화천 및 삼화천에서 조사된 관속식물은 72과 175속 201종 38변종 2품종 1재배종으로 총 242분류군이 확인되었으며, 이중 식재종이 92분류군(38.0%), 자생종이 150분류군(62.0%)을 차지하였다. 또한, 염생식물은 5과 9속 12종 1변종으로 총 13분류군(5.4%), 귀화식물은 17과 41속 45종 1변종으로 총 46분류군이 확인되었으며, 19.0%의 귀화율과 17.0%의 도시화지수로 분석되었다.

• 한국자원식물학회지
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• 제23권6호
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• pp.526-534
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• 2010

Genomes of clusters of related eukaryotes are now being sequenced at an increasing rate. In this paper, we developed an accurate, low-cost method for annotation of gene prediction and exon-intron structure. The gene prediction was adapted for delta 1-pyrroline-5-carboxylate-synthetase (p5cs) gene from China wild-type of the halophytic Leymus chinensis (Trin.), naturally adapted to highly-alkali soils. Due to complex adaptive mechanisms in halophytes, more attentions are being paid on the regulatory elements of stress adaptation in halophytes. P5CS encodes delta 1-pyrroline-5-carboxylate-synthetase, a key regulatory enzyme involved in the biosynthesis of proline, that has direct correlation with proline accumulation in vivo and positive relationship with stress tolerance. Using analysis of reverse transcription-polymerase chain reaction (RT-PCR) and PCR, and direct sequencing, 1076 base pairs (bp) of cDNA in length and 2396 bp of genomic DNA in length were obtained from direct sequencing results. Through gene prediction and exon-intron structure verification, the full-length of cDNA sequence was divided into eight parts, with seven parts of intron insertion. The average lengths of determinated coding regions and non-coding regions were 154.17 bp and 188.57 bp, respectively. Nearly all splice sites displayed GT as the donor sites at the 5' end of intron region, and 71.43% displayed AG as the acceptor sites at the 3' end of intron region. We conclude that this method is a cost-effective way for obtaining an experimentally verified genome annotation.

• Journal of Forest and Environmental Science
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• 제30권1호
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• pp.120-125
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• 2014

Halophytes are plants of saline habitats that grow under conditions that may vary in extremes of temperatures (freezing to very hot), water availability (drought to water logging) and salinity (mild to almost saturation). Halophytes may also face sudden micro-environmental variations within their habitats. In this study, we examine some of the factors that determine the ability of seeds of Artemisia fukudo to germinate when conditions are optimal for seedling growth and survival. Germination percentage was the highest at 77.5% when treated in 0% seawater and seedling growth was best in 0% seawater plug cell tray. Physiological quality (chlorophyll contents) and mean germination time were best in the condition in which seeds were treated with 0% seawater (control). Germination performance index in the control group showed the highest value to 3.8. Seedling growth, seedling vigor index in the length of seedling growth andthe relative growth rate were the highest values of 0.76 cm, 1.43, 0.0099, respectively.

• 한국균학회지
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• 제40권2호
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• pp.86-92
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• 2012

염생식물인 칠면초, 갈대, 갯질경, 해홍나물을 고창갯벌로부터 채집하였으며, 39점의 내생진균을 염 습지에 자생하고 있는 염생식물의 뿌리로부터 분리하였다. 내생진균들은 ITS1, 5.8s와 ITS2를 포함하는 ITS-rDNA 영역에 의해 분석되었다. 다양한 내생진균은 Eurotiales (36%), Pleosporales (26%), Hypocreales (18%), Incertae sedis (8%), Glomerellales (5%), Sordariales (2%), Xylariales (2%), Capnodiales (3%)등 8종류 목에 속하는 것으로 확인되었다. 39점의 내생진균은 속(genus)단계에서 Alternaria, Aspergillus, Chaetomium, Cladosporium, Colletotrichum, Coniothyrium, Dothideomycete, Fusarium, Gibberella, Macrophoma, Penicillium, Pestalotiopsis, Phaeosphaeria, Phoma, Pleosporales, Pseudozyma, Talaromyces, 그리고 Termitomyces에 포함되는 것으로 분석되었다. 그리고 내생진균은 Eurotiales목의 Penicillium속이 가장 많이 분포하고 있는 것으로 분석되었다.

• 한국수산과학회지
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• 제39권spc1호
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• pp.180-188
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• 2006

We examined the effect of salt marsh vegetation on the benthic environment in macro-tidal flats at Donggeomdo, Ganghwa, on the west coast of Korea. Nine stations were established along a transect across the tidal flats, including salt marsh, and field sampling was conducted monthly from July 1997 to June 1998. During the study period, environmental parameters fluctuated as follows: salinity, 15.0 to 28.2 psu, exposure time, 613 to 702 hr/m, inundation time, 28 to 117 hr/m, sediment organic carbon, 0.71 to 1.34%, nitrogen, 0.07 to 0.16%, sulfur from 0.07 to 0.22%, mean grain size from 6.3 to $6.9{\phi}$, water content from 19.4 to 44.4%, water temperature from 4.4 to $20.4^{\circ}C$ and temperature of the surface sediments, 2.7 to $31.1^{\circ}C$, in total, seven halophyte species were sampled and found to be vertically distributed across the tidal levels. Carer scabrifolia, Suaeda maritima, Limonium tetragonum and Phragmites communis dominated the upper zone of the salt marsh, Juncus haenkei, Triglochin maritimum and P. communis dominated the middle zone, and Suaeda japonica predominated in the lower area. Principal component analysis (PCA) and non-metric multi-dimensional scaling revealed that the zonation of halophytes was related to environmental variables such as salinity and exposure time. The halophyte communities were likely related to the organic content of the surface sediment.

• 환경영향평가
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• 제21권4호
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• pp.509-523
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• 2012

This basic study is to present the methods and raw data for carrying out to objectively assess an ecosystem by developing the plant ecological indices. The results are as follows. The plant ecological indices were 7 units including RI (Rare Index), EI (Endemic Index), SI (Specific Index), NI (Naturalized Index), UI (Urbanized Index), Pte-Q (Pteridophyta Index) and HI (Halophytes Index). The vascular plants in Mt. Waryong, Mt. Bongmyeong and Bito island were summarized as 471 taxa including 104 families, 294 genera, 419 species, 3 subspecies, 41 varieties and 8 forms. We surveyed 5 taxa of rare plants, 7 taxa of endemic plants, 38 taxa of specific plants by floristic region, 40 taxa of naturalized plants, 1 taxa of invasive alien plant, 19 taxa of pteridophyta and 14 taxa of halophytes. Bito island had the highest RI was 1.2%. Mt. Bongmyeong was 1.4% higher than other sites. If the SI of 2.9% was the highest in Bito island. Bito island was the highest NI, and the UI was the hightest in Mt. Waryong. Mt. Bongmyeong was the lowest NI and UI. In Mt. Waryong and Bito island, the highest DI was 9.1%. The Pte-Q of Mt. Waryong was 1.3 higher than the other sites. Bito island, the highest HI was 8.1%. Overall, 1.5% of RI, 1.9% of EI, 2.3% of SI, 8.5% of NI, 14.3% of UI, 9.1% of DI, 1.0 of Pte-Q and 3.0% were analyzed with HI.