• 한국어병학회지
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• 제28권1호
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• pp.53-62
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• 2015

Here, we report the physiological changes and growth in disk abalone, Haliotis discus discus, in relation to dietary supplementation with citrus pomace (CP) 6%, Ecklonia cava residue (ECR) 6%, and CP + ECR (3% + 3%). The composition and nutrient content, survival rate and growth rate were measured 0, 4, 8 and 12 weeks after feeding the supplemented diets of CP and/or ECR. Moreover, the experiment of low salinity stress (25psu) for environmental resistance was examined for a period of 48 hours after feeding the supplemented diets for 12 weeks. The activities of superoxide dismutase (SOD), catalase (CAT), lysozymes, respiratory burst, and phenoloxidase were measured. The moisture content and crude protein condition of the body were increased with the addition of ECR only (P<0.05). We observed higher levels of survival in the experimental group compared with the control group. Moreover, the growth disk abalone that were fed a diet containing ECR was higher compared with the control group. However, the growth of abalone fed a diet containing CP was similar to the control group. With a rearing condition of low salinity stress, survival rate and lysozyme activity were increased in the ECR group compared with the control group. Dietary ECR reduced the level of CAT activity to approximately 30% of the control, however the level of CAT activity in the ECR group was similar to the start level of the previous stress. These results suggest that dietary ECR gives rise to an enhanced immunity in disk abalone, as a result of the decrease in CAT and lysozyme activity in particular. Accordingly, the growth and survival rate were increased by feeding an ECR-supplemented diet in the rearing of disk abalone, Haliotis discus discus.

• 한국양식학회지
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• 제11권4호
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• pp.465-474
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• 1998

참전복(Haliotis discus hannai) 양성과정중 치패단계에서 발생된 패각 괴사증의 조사결과는 다음과 같다. 괴사된 전복패각에서 간균 형태(rod type)와 이질세포를 갖는 남조류가 관찰되었다. 그러나 주사전자현미경(SEM)의 자료에 근거하면 패각과 사증의 원인생물은 천공성 남조류, Mastigocoleus sp.로 나타났다. 천공성 남조류에 감염된 전복의 패각은 녹색에서 회백색으로 탈색되었고, 4~6번째 호흡공은 부서지기 쉬운 상태가 되었다. 양성 60일 후, 패각 괴사증은 갈색의 작은 집락이 산재되어있는 호흡공에서 주로 발생하였으며, 양성 후 3년까지 지속되었다. 이러한 패각 괴사증은 바다 채롱식 양식장에서 보다는 육상 수조식 양식장에서 주로 발생하였으며, 참전복의 성장에 커다란 영향을 미친 결과 3년산 참전복의 각장이 16mm였다.

• 한국양식학회지
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• 제16권4호
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• pp.223-228
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• 2003

전복 치패를 부착기질로부터 분리시키는 박리는 전복양식에 있어서 치패의 선별과 밀도 조절을 위해 필수적인 작업이다. 현재 물리적인 방법과 마취제를 이용한 방법이 개발되어 있으나 치패의 손상이 없는 마취제에 의한 박리가 보다 효과적인 것으로 판단되고 있다. 따라서 본 연구는 요소비료($Co(NH_2)_2$)를 마취제로 이용하여 요소비료의 농도와 수온 등 물리적 요인이 까막전복 Haliotis discus의 박리율과 회복률에 미치는 영향을 조사함으로써 경제적이며 효과적인 박리 기술을 개발하고자 하였다. 실험 결과 9∼15%의 요소비료를 해수에 용해시키고 이를 자연 해수와 동일한 수온으로 상승시킨 후 박리를 실시하였더니 3분 이내에 97% 이상이 박리되었으며 치패의 회복률도 역시 높았다. 또한 요소비료 농도와 수온은 높을수록 박리율도 높았으나 치패의 회복률은 낮았다. 결론적으로 본 연구를 통하여 농업용 요소비료를 전복의 박리에 사용할 수 있음을 확인하였으며, 다른 종류의 전복에서도 본 기술의 적용이 가능할 것으로 생각되며, 본 기술은 현재 사용되고 있는 모든 전복 박리방법이나 전복 마취제로 쓰이는 화학약품을 대체할 수 있는 방법으로 판단된다.

• 한국패류학회지
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• 제32권4호
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• pp.241-247
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• 2016

Macrophage Migration Inhibitory Factor (MIF) are well-defined role as unique cytokine and critical mediator in acute and chronic inflammatory diseases, autoimmune diseases. In this study, we isolated and characterized a full-length of MIF cDNA from the abalone (Haliotis discus hannai). The full-length cDNA of abMIF was of 1264 bp, consisting of a 5'-terminal UTR of 143 bp, an open reading frame of 360 bp and a 3-terminal UTR of 761 bp. The abalone MIF cDNA encodes a 119-amino acid polypeptide with a calculated molecular mass of 13.4 kDa and isoelectric point of 9.07. Multiple alignments and phylogenetic analysis with the deduced abalone MIF protein and showed strong homology with disk abalone (Haliotis discusdiscus). The deduced amino acid sequence of abMIF exhibited homology with other reported MIFs, such as 80%, with that of other disk abalone H. discus discus MIF gene. Quantitative real-time PCR (qRT-PCR) analysis indicated that abMIF was highly expression observed in hapatopacreas, intestine, foot, and gonad of normal conditioned abalone. Even though AbMIF mRNA level in hemocytes was low under the normal condition, it was sharply up-regulated and reached the maximum at 6 h post-infection with Vibrio parahaemolyticus, and then decreased at 24 h post-infection. This result indicates that abMIF plays an important role in responding in the innate immune system.

• 한국패류학회지
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• 제28권1호
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• pp.37-43
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• 2012

북방전복, Haliotis discus hannai의 3배체를 제온자극으로 유도하고 부화유생인 trochophore를 이용하여 염색체 표본을 만들었고, 유도된 3배체와 정상 2배체 북방전복은 실내 유수식의 동일 환경에서 51개월 동안 사육되었으며 채취된 혈구를 채취하여 DNA 함량 측정에 사용되었다. 2배체 및 유도된 3배체의 염색체 수를 조사한 결과, 2배체 염색체 수는 2n = 36으로 나타났고, 3배체의 경우에는 3n = 54로 나타나 3배체는 2배체에 비해 1.5배의 염색체 수를 나타내었다. Flow cytometry로 인간의 백혈구를 control로 하여 북방전복의 DNA 함량을 측정한 결과, 북방전복의 DNA 함량은 1.743 pg/cell이었으며 3배체 북방전복의 DNA함량은 2배체 전복의 1.49배의 DNA 함량을 나타내어 3배체 특성인 모계 2n DNA 함량과 부계 n DNA함량을 나타내었다.

• 한국해양생명과학회지
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• 제6권1호
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• pp.23-30
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• 2021

북방전복 Haliotis discus hannai 웅성생식세포의 분화과정과 정자의 형태를 미세구조적으로 기재하였다. 정자의 분화과정은 정원세포기, 정모세포기, 정세포기 및 정자기의 4단계로 구분하였다. 정원세포기에서 정모세포기로의 분화과정은 형태학적 변화가 크지 않았다. 그러나 정자변태과정 동안 염색질 응축, 핵의 형태 변화, 첨체와 중편 및 편모 형성 등의 급격한 형태학적 변화를 나타냈다. 북방전복의 정자는 두부, 중편 및 미부로 구성되며, 두부의 길이는 약 5.3 ㎛로 전자밀도가 높은 핵과 총알형의 첨체로 이루어져 있었다. 중편은 기저체와 미토콘드리아로 구성되어 있었으며, 기저체를 중심으로 5개의 미토콘드리아가 한 층으로 배열되어 있었다. 미부의 횡단면은 "9+2"의 미세소관 구조를 보였다. 이러한 형태 및 구조적 특징은 북방전복의 정자는 원시형(primitive type) 정자임을 보여주는 결과이다.

• 한국해양생명과학회지
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• 제2권2호
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• pp.83-89
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• 2017

In abalones, interspecific hybridization has been suggested as a possible means to increase production and desired traits for the industry. In Korea, Haliotis gigantea is considered a species with a larger size and higher temperature tolerance than H. discus hannai. However, H. discus hannai is considered the most valuable and popular fishery resource due to its better acceptance and higher market prices. Thus, viable interspecific hybrids have been produced by artificial inseminating H. gigantea eggs with H. discus hannai sperm. However, the reciprocal hybrid cross was not successful. In this study, the hybridity and the growth and thermal tolerance performance of the interspecific hybrids were examined. A combination of various assays revealed maximum growth occurrence at 21℃ and the higher growth rate in the hybrids than that of H. discus hannai parent. In addition, the growth and survival at high-temperature (28℃) of the hybrids was equivalent to that of the highly tolerant H. gigantea parent, suggesting new possibilities to overcome the mass mortality in H. discus hannai during high temperature periods of summer season in Korea. Furthermore, the induced interspecific hybrid status was confirmed by the presence of species-specific bands for each parental species of the random amplified polymorphic DNA (RAPD) profiles using universal rice primer (URP), which could be used as speciesspecific markers to distinguish the hybrids and their parental species.

• 한국양식학회지
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• 제12권4호
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• pp.317-322
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• 1999

A growth trial was conducted to investigate the utilization of wheat germ meal as a protein source of formulated diet for juvenile abalone (Haliotis discus hannai). Four replicate groups of the abalone average weighing 150mg were fed one of four isonitrogenous (33%) and isolipidic (6%) diets containing 0%, 10%, 20%, or 30% wheat germ meal for 18 weeks. In addition, these formulated diets were compared with commercial diet. Survival rate, weight gain, soft body weight , and shell growth of abalone fed diets containing 0%, 10%, 20%, or 30% wheat germ meal were not different (P>0.05) from those of abalonn fed the control diet and commercial diet. There were no significant differences (P>0.05) in soft body composition of moisture, protein and lipid. It si concluded that wheat germ meal were be used as a partial protein source of formulated diet for juvenile abalone.

• 한국수산과학회지
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• 제47권2호
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• pp.154-159
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• 2014

The increasing of atmospheric $CO_2$ are changing the pH (ocean acidification) and temperature of the sea. Although the effects of ocean acidification on calcifying organisms have well-documented, only a few studies have examined the combined effects of ocean acidification and elevated temperature. This study investigated the effects of ocean acidification and elevated temperature for 2100 on survival and growth of juvenile abalone, Haliotis discus hannai. Ocean acidification was simulated by bubbling $CO_2$ into seawater at concentrations of 1,000 and 1,500 ppm, and temperature was set at room temperature $+2^{\circ}C$. Neither $CO_2$ nor temperature had a significant effect on survival of abalone, while both significantly affected growth. There was no significant interaction between the two factors. Shell length can be used as a growth index of abalone to access the impacts of ocean acidification and elevated temperature.

• 한국수산과학회지
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• 제47권6호
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• pp.796-800
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• 2014

A feeding trial was conducted to investigate the growth and body composition of juvenile sea cucumber Apostichopus japonicus in integrated culture with abalone Haliotis discus hannai or rockfish Sebastes schlegeli. Triplicate groups of sea cucumber averaging $1.2{\pm}0.05g$ were cultured alone or with abalone or rockfish for 12 weeks. Survival of sea cucumber was not affected by co-culturing (P>0.05). Weight gain of sea cucumber cultured with rockfish was significantly higher than that of sea cucumber cultured alone (P<0.05), and did not differ from that of those cultured with abalone (P>0.05). These findings indicate that co-culturing sea cucumber with rockfish effectively improves the growth of sea cucumber.