• Journal of Animal Science and Technology
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• v.46 no.3
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• pp.307-314
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• 2004

Xenotransplantation of porcine organs has the potential to overcome the severe. shortage of human tissues and organs available for human transplantation. The swine represents an ideal source of such organs because of their plentiful supply and their numerous anatomical and physiological similarities to the human. However, this procedure also carries with a number of safety issues relating to the zoonotic infections. Porcine endogenous retrovinJses(PERVs), \Wich are germ line transmitted and persist without symptoms in the pigs, are most concerning zoonotic viroses. In order to analyze the prevalence of PERV in domestic pigs, four kinds of pigs'(Landrace, Berkshire, Yorkshire, and Duroc) genomic DNA were isolated from their hair follicles. PCR analysis was carried out for detection of PERVs using subgroup A/B/C and E pol sequence primers. All pigs (20 heads) tested had high copy number of PERVs within genomes. Subgroup A/B/C and E pol gene sequences from 20 isolates were determined by direct sequencing. Sequence analysis showed pol sequences are highly conserved among intra- and inter-subspecies(99.l and 98.8%, respectively). As a first report of PERV prevalence in Korea pigs, our data would be the basic concepts of PERV transmission study in xenotransplantation.

• Journal of Animal Science and Technology
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• v.58 no.11
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• pp.40.1-40.5
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• 2016

Background: Currently about 26,000 horses are breeding in Korea and 57.2% (14,776 horses) of them are breeding in Jeju island. According to the statistics published in 2010, the horses breeding in Jeju island are subdivided into Jeju horse (6.1%), Thoroughbred (18.8%) and Halla horse (75.1%). Halla horses are defined as a crossbreed between Jeju and Thoroughbred horses and are used for horse racing, horse riding and horse meat production. However, little research has been conducted on Halla horses because of the perception of crossbreed and people's weighted interest toward Jeju horses. Method: Using 17 Microsatellite (MS) Markers recommended by International Society for Animal Genetics (ISAG), genomic DNAs were extracted from the hair roots of 3,880 Halla horses breeding in Korea and genetic diversity was identified by genotyping after PCR was performed. Results and conclusion: In average, 10.41 alleles (from 6 alleles in HTG7 to 17 alleles in ASB17) were identified after the analysis using 17 MS Markers. The mean value of $H_{obs}$ was 0.749 with a range from 0.612(HMS1) to 0. 857(ASB2). Also, it was found that $H_{\exp}$ and PIC values were lowest in HMS1 (0.607 and 0.548, respectively), and highest in LEX3(0.859 and 0.843, respectively), and the mean value of $H_{\exp}$ was 0.760 and that of PIC was 0.728. 17 MS markers used in this studies were considered as appropriate markers for the polymorphism analysis of Halla horses. The frequency for the appearance of identical individuals was $5.90{\times}10^{-20}$ when assumed as random mating population and when assumed as half-sib and full-sib population, frequencies were $4.08{\times}10^{-15}$ and $3.56{\times}10^{-8}$, respectively. Based on these results, the 17 MS markers can be used adequately for the Individual Identification and Parentage Verification of Halla horses. Remarkably, allele M and Q of ASB23 marker, G of HMS2 marker, H and L of HTG6 marker, L of HTG7 marker, E of LEX3 marker were the specific alleles unique to Halla horses.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.7
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• pp.410-416
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• 2017

The silver generation in our aging society will rapidly emergeas a new consumer group and the service industry used by silver customers will develop going forward. The beauty service sector is one of the most needed ones for the qualitative improvement of life for the silver generation. In this research, ananalysis was conducted on 244 persons of the silver generation living in Seoul, in order to examine the factors affecting the quality of the beauty service for the silver generation and their relationships with customer loyalty. For the statistical treatment of the empirical analysis, the SPSS 23.0 and AMOS 23.0 statistical programs were used. The results of the analysis showed that the service quality perceived by the silver customers who used the beauty service had significant effects on trust and flow, which are the relationship qualities, and that trust and flow had positive effects on customer loyalty. Also, it was found that, in the relationship between service quality and customer loyalty, trust had mediating effects on reliability, responsiveness, empathy, and assurance, and that flow had mediating effects on reliability, responsiveness, empathy, assurance, and tangibility. The results of this research are significant in that they present the basic data enabling the beauty industry to play an important role for the silver generation, under the present circumstances in which the beauty service industry has minimal educational programs exclusively available for the silver generation.

• Journal of Life Science
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• v.19 no.12
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• pp.1777-1786
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• 2009

Thymosin ${\beta}4$, a small protein containing 43 amino acids, has multi-functional roles in cell physiology. It was first identified as a thymic maturation factor and recently has been shown to accelerate wound healing, hair growth, angiogenesis, tumor growth, and metastasis. It was also reported to play a key role in developing organs, including the nervous system and heart. Thymosin ${\beta}4$ induces the expression of vascular endothelial cell growth factor (VEGF), laminin-5, and other important biologically active genes. Using tissue microarray analysis, we investigated the expression patterns of thymosin ${\beta}4$ and VEGF in various normal human adult tissues. Thymosin ${\beta}4$ was highly expressed in the liver, pancreas, ductal epithelium of the salivary gland, and heart, and moderately expressed in the skin, lung, spleen, lymph node, thymus, ureter, and blood endothelial cells in both the lung and adrenal gland. The expression of VEGF generally co-localized with thymosin ${\beta}4$ and VEGF was highly expressed in the pancreas, ureter, mammary gland, liver, esophagus, and blood endothelial cells in both the lung and adrenal gland. These results suggest that thymosin ${\beta}4$ plays an important role in the function of various organs and since the expression pattern of thymosin ${\beta}4$ co-localized with VEGF, part of that function may be to induce or maintain angiogenesis.

• Applied Microscopy
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• v.42 no.1
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• pp.1-7
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• 2012

The possibility of nanoparticles (NPs) in biotechnology had been discussed by biomedical investigators. Here we report to suggest a solution and problems when using electron microscopy to determine the distribution of quantum dots (QDs) nanoparticles that penetrate skin. The results of this study showed that NPs were able to penetrate stratum corneum (SC) and sebocyte via hair follicle. However, we have found artifacts such as nanoparticles that are produced from combination of free fatty acid and osmium tetroxide during specimen preparation. It is usually difficult to identify NPs. Therefore, we tried to resolve these problems by comparing the cross-correlation image pattern that are derived from the images of sample that had been processed differently. This method can contribute to more accurate interpretation and minimal errors during the analysis using quantum dot as tracer.

• The Journal of Korean Physical Therapy
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• v.11 no.3
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• pp.81-87
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• 1999

The purpose of this study was to determine the effect of electrical stimulation on the number of MCs and percent of degranulated MCs in rat skin. Twelve male Sprague-Dawley rats were divided into two group; electrical stimulation group (n=6) and control group (n=6). Each animals hair on the back was removed. The electrical stimulation group received an positive rectangular pulsed electrical stimulation, while the control group was given the same treatment without electricity. The biopsy specimens were fixed in formalin, embedded in paraffin and stained with toluidine blue-nuclear fast red and alcian blue-safranin O. respectively. The MCs were counted using a light microscope and computerized image analysis system and calculated as the density and the percent. A t-test showed a significantly higher density of MCs in the electrical stimulated rats than control rats(p<0.05), and the percent of degranulated MCs in the electrical stimulated rats was higher than in the control rats (p<0.05) in toluidine blue stained sections. The density of MCs was significantly higher in the electrical stimulated rats than the control rats in alcian blue-safranin O Stained sections (P<0.01). An analysis of variance showed that the densities of CTMCs was significantly lower than the densities of MMCs and mixed MCs in electrical stimulated rat in alcian blue-safranin O Stained sections (p<0.05). These results suggest that the electrical stimulation may have potential for increasing the number of MCs and lead to degranulate the MCs in rat skin.

• Journal of Life Science
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• v.23 no.12
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• pp.1428-1435
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• 2013

The aim of this study is to investigate the melanogenic effect of Oenanthe javanica ethanolic extracts (OJE) containing quercetin and kaempferol in melanoma cells (B16F1). In order to determine whether OJE inhibits melanin synthesis at the cellular level, the melanoma cells were cultured in the presence of different concentrations of OJE. In the present study, the antioxidant effects of OJE on DPPH radical scavenging, power reduction, lipid peroxidation, and DNA oxidation were evaluated in a cell free system. Furthermore, the effect of OJE on the production of melanin was determined by dopaquinone (DOPA) assay and tyrosinase activity. In addition, the protein expression of tyrosinase, as well as antioxidant enzymes such as superoxide dismutase (SOD)-1, SOD-2 and glutathione reductase (GSH), were examined using Western blot analysis. In this study, it was observed that OJE exhibited an inhibitory effect on lipid peroxidation and blocked the DNA oxidation induced by the hydroxyl radical produced by Fenton's reagent. OJE increased melanin synthesis above 50 ${\mu}g/ml$ and tyrosinase activity was detected above 50 ${\mu}g/ml$. In Western blot analysis, OJE increased the expression levels of tyrosinase, SOD-1, SOD-2, and GSH in a dose-dependent manner. These findings indicate that OJE with antioxidant activity can regulate the tyrosinase activity and melanin production in melanocyte, suggesting that it could promote the development of black hair as well as protect skin from oxidative stress.

• Journal of Fashion Business
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• v.15 no.6
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• pp.113-133
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• 2011

The purpose of this study is to establish academic identity as well as produce human resources that industries need by grasping curriculum of the departments related beauty focusing on Korean colleges (junior colleges and four year course colleges), analyzing examples of advanced countries and suggesting their developmental direction. For the research method, the reference period of curriculum in Korean colleges was from Jul. 05th, 2011 to Aug. 05th, 2011. the investigation method is as follow: first, the curriculum of the departments related to beauty which were registered in the web sites were investigated. Second, the documents were received by fax and Email from each department after calling it. Third, questions and answers were done in reference to majors on the phones. these methods are intended for 65 junior colleges and 16 four year course colleges (total 81 ones). It clarified that lifelong education centers and the graduate courses were ruled out. the statistical analysis about data which were investigated like this were done by the frequency analysis. The results which examined the major subjects of Make-up focusing on Korea and foreign countries are as follow: First, the result which examined the major and theory subjects of Make-up has found that there are the human body theory, the equipment theory, the marketing theory are the management theory in Korean junior colleges and physiology/chemical, management, marketing and equipment product were treated in four year course colleges of Korea. As for foreign colleges, the subjects related to history and culture are mainly treated as the theory courses and their purpose is to cultivate basic knowledge of the fields which are mainly connected to Make-up including plays, movies, TV and studios. Second, the result which examined practice subjects of Make-up major has found that practice subjects of the total ones are of great importance in junior colleges. And it could be found that the Make-up field is of little importance as it is not independent and exists with the hair and skin fields in one department in the four year course colleges. Especially, in the foreign countries, the more detailed and professional subjects including classes which treat digital media, studio technologies, production, wigs and the special effects were treated by the field class system and these characteristics implies that Korean curriculum should be changed in the future. Based on this analytical results of investigation, this researcher tried to propose the developmental direction of Korean curriculum in the future.

• Journal of Physiology & Pathology in Korean Medicine
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• v.23 no.1
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• pp.76-83
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• 2009

Rehmannia Radix Preparata (RRP) used to nourish Eum and enrich blood for consumptive fever, aching, and limpness of the loins and knees, and to replenish essence for tinnitus, premature greying of beard and hair. In the present study, we studied about the protective effect of RRP on hydrogen peroxide-induced oxidative stress in human vascular endothelial cells. ECV304 cells were preincubated with RRP (100, 200, 300 and $400{\mu}g/m{\ell}$) for 12hr and then treated with $600{\mu}M$ $H_2O_2$ for 12hr. The protective effects of RRP on $H_2O_2$-induced apoptosis in ECV304 cells was determined by using MTT assay, FDA-PI staining, flow cytometric analysis, caspase-3 activity assay, ROS assay and western blot. The results of this experiment showed that RRP inhibited $H_2O_2$-induced apoptosis and ROS production in ECV304 cells. Moreover, RRP increased ERK activation that decreased in $H_2O_2$-treated ECV304 cells, and inhibited p38 and JNK activation. Furthermore, RRP increased expression of heme oxygenase-1 (HO-1) in $H_2O_2$-treated ECV304 cells. Also, HO-1 protein expression induced by RRP was reduced by the addition of ERK inhibitor (PD98059) in $H_2O_2$-treated ECV304 cells. These results suggest that protective effect of RRP on $H_2O_2$-induced oxidative stress in ECV304 cells may be associated with increase of ERK activation and HO-1 protein, and reduction of p38 and JNK activation.

• Applied Microscopy
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• v.39 no.2
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• pp.149-156
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• 2009

Fine structure of the dry adhesion system in the tarsal appendages of the jumping spider Plexippus setipes (Araneae: Salticidae) with examined using field emission scanning electron microscope (FESEM). The jumping spiders have the distinctive attachment apparatus for adhesion on smooth dry surface without sticky fluids. They attach to rough substrates using tarsal claws, however attachment on smooth surfaces is achieved by means of a tuft-like hair called a scopula. All eight legs have the scopulae with a pair of claws on the tip of feet, and each scopula is composed of two groups of setae that are capable of dry adhesion on smooth surface. The apex of each seta is flattened pad bearing many specialized adhesive setules on one side. The cuticular sensillae are interspersed at the dorsal surface of the seta. It has been revealed by this research that the contact area of the setule is always a triangular shape, and these cuticular surfaces are connected by the elongated stalks from the underlying setae. Moreover, adhesion between the numerous setules and the setae was prevented by the microscopic hairs, since these were interspersed on the upper side of the setae.