• Journal of Sericultural and Entomological Science
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• v.28 no.2
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• pp.38-47
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• 1986

Silkworm(Bombix mori) were reared with modified artificial diets which were mixed with, as additives, leaf powders of Erigeron canadensis L., Cassia tora L., Cyperus anuricus Var.Laxus and Vigna Sinensis NEDL. The effects of additives on silkworm characteristisc of tested plants were summarized as follows ; 1. About 2-5% addition on dry weight base of leaf powders of E. canadensis, C. tora, C. anuricus or V.sinensis to the basic artificial diet promoted feeding response and digestion and resulted in good practical silkworm characteristics. The addition of V.sinensis and C.anuricus showed especially good effects. 2. The syneristic effect between different plant species was not recognized based on the feeding response and digestion of silkworm reared with various combinations of 2-4 different plant additives. 3. Electrophoretic zymograms of estrase, protease and phosphatase on haemolymph, intestine and silkgland were significantly different among treatments. In general, 1 or 2 more electrophoretic bands were detected when feeding response and digestion were promoted. 4. Contents of starch, crude fat, crude protein and inorganic base were apparently higher in the tested plants than in mulberry leaves. However, no volatile ingredent which is directly realted with feeding response was identified.

• Korean journal of applied entomology
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• v.34 no.4
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• pp.287-294
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• 1995

Studies were carried out to elucidate the efficacy of exogeneous 20-hydroxyecdysone treatment on terminating pupal diapause in the fall webworm. Hyphantria cunea Drury. And the difference was also investigated between normal adult development and post-diapause development after 20-hydroxyecdysone treatment. In the diapause termination rate of pupae treated with 20-hydroxyecdysone after storage for various periods at 16L:8D and $25\pm$$1^{\circ}C$, at the highest rate was observed from the group stored for the longest period and the lowest rate from those stored for 1.5 months. The time needed for adult emergence was inversely proportional to the chilling (at $0\pm$$1^{\circ}C$) period, and the longer its exposure period at low temperature, the higher its sensitivity to 20-hydroxyecdysone treatment. Pupal diapause duration was almost the same, regardless of storage period in the total darkness or at the photoperiod of 16L:8D, and also they successfully emerged to adult even without any experience at low temperature. The oxygen consumption rate in normally developing pupae showed nearly a typical U-form. But, that of diapausing pupae treated with 20-hydroxyecdysone slowly increased and jumped 14 days after the treatment. Pupal diapause began before formation of adult tissues, an a timing of adult tissue formation coincided with ascending timing of the metabolic rate in both normally developing pupae and diapausing pupae treated with 20-hydroxyecdysone. The diapausing pupae treated with 20-hydroxyecdysone was similar to normally developing pupae in band patterns of proteins from haemolymph or fat body.

• Journal of Sericultural and Entomological Science
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• v.50 no.2
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• pp.145-149
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• 2012

Cecropin is a well-studied antimicrobial peptide that play important role as key factor in insect humoral immunity. In this study, cecropin-like antimicrobial peptide was isolated and purified from the larval haemolymph of immune-challenged japanese oak silkworm, Antheraea yamamai. To isolate antimicrobial peptide, we separated and compared acidic extracted hemolymph protein bends between control and immune-challenged larvae using SDS-PAGE analysis. In the immune hemolymph extract, but not of non-immune hemolymph, we detected differential expressed peptide band with molecular mass 4,223.01 Da. To understand this peptide better, we successfully purified this peptide using cation exchange chromatography and gel permeation chromatography. Its N-terminal amino acid sequence obtained by Edman degradation evidenced a significant degree of identity with other lepidopteran cecropins. The purified A. yamamai cecropin-like peptide showed a broad spectrum of activity against fungi, Gram-negative and Gram-positive bacteria.