• The Korean Journal of Zoology
• /
• v.23 no.1
• /
• pp.1-12
• /
• 1980

Changes and possible origin of haemolymph proteins during the cuticle formation and hardening are determined by means of acrylamide gel electrophoresis and immunodiffusion. The results by acrylamide gel electrophoresis showed at least 19 protein bands in the haemolymph and 13 fractions in the fat body with relatively constant pattern during the period of cuticle formation and hardening. Both haemolymph and fat body proteins are generally characterized by the presence of three to four heavy stained bands and several thin bands near the top region of the gel. At least over five haemolymph proteins are constantly present during this period. Immunodiffusion tests show that of total eight to nine pupal haemolymph proteins two proteins were already detected in the fat body before pupation and other two proteins were also found in the fat body immediately after pupation, suggesting fat body as possible source of these two haemolymph proteins.

• Journal of Sericultural and Entomological Science
• /
• v.41 no.2
• /
• pp.75-81
• /
• 1999

The storage protein-like protein has been purified from the 5th instar larval haemolymph of the Chinese osk silkwom, Antheraea pernyi, and the preparation was shown to be homogeneous by 7.5% native-PAGE. The molecule was consisted of a single subunit with a molecular weight of 80K, but the number of the subunits was not determined. The protein was defied as glycoprotein by Schiff's regent stining. Rabbit antibody prepared against the purified protein crotein crossreacted with the 5th instar larval haemolymph proteins of Antheraea pernyi and antheraea yamamai, but not with those of Bombyx mori and Bombyx mandarina.

• International Journal of Industrial Entomology and Biomaterials
• /
• v.14 no.1
• /
• pp.37-44
• /
• 2007

A study was made of the haemolymph protein spectrum of mulberry silkworm (Bomhyx mori L.) from the first larval instar to imago. Horizontal starch gel electrophoresis was used. Sixteen races and eight F1 interracial hybrids, raised in Bulgaria, were analyzed. During the ontogenesis, a total of 17 protein bands (15 cathodic and 2 anodic) were detected. Distinct dynamics in the haemolymph protein spectrum was observed, in result of different expression during the individual development associated with the processes of growth, histolysis and histogenesis. Based on the ontogenetic dynamics found, a correspondence was assumed between some proteins detected by us using the starch gel electrophoresis and major haemolymph proteins (SP1, SP2, MHPs and Vg) detected by other authors using the polyacrilamide gel electrophoresis. Intraracial and interracial polymorphism was observed in four protein zones. The effect of four polymorphic loci with codominant and null alleles was suggested.

• International Journal of Industrial Entomology and Biomaterials
• /
• v.12 no.2
• /
• pp.75-80
• /
• 2006

Changes in the levels of protein and free amino acids in the haemolymph of three selected races of the silkworm, Bombyx mori viz., PM, $NB_4D_2$ and $CSR_2$, were investigated during 4th moult, 5th instar and pupal period. The levels of total protein in the haemolymph, increased from first day of 5th instar till sixth day. From seventh day till spinning, the protein levels decreased in all the three races. A sustained decrease in the haemolymph proteins was observed during the pupal development in all the three races. The levels of free amino acids, which were high during 4th moult, declined through the 5th age of larval development till spinning. PM showed a relatively higher free amino acid level (3.192 mg/ml) in haemolymph followed by $NB_4D_2$ (2.601 mg/ml) and $CSR_2$ (2.35 mg/ml). The free amino acid levels decreased gradually from prepupal stage but increased again at the end of pupal period. Racial differences in the changes in the levels of protein and free amino acids in the haemolymph were observed in the larvae and pupae when subjected to two high temperature regimes of $30^{\circ}C$ and $35^{\circ}C$. The results showed that high temperature induces specific changes in the metabolism (reversible thermal stress) that have different adaptive value in different races of the silkworm. Relatively higher increase in the free amino acid levels in the haemolymph of Pure Mysore presumably provides protective cover to tissues against high temperature by an increase in osmolarity and reduction in evaporative water loss. The absence of such a mechanism may be responsible for temperature susceptibility of the bivoltine races like $NB_4D_2$ and $CSR_2$.

• International Journal of Industrial Entomology and Biomaterials
• /
• v.3 no.1
• /
• pp.89-92
• /
• 2001

The effect of topical application with 5,10 and 15 $\mu$g/ ml androstenedione and methyltesterone to the fifth stadium larvae of the si1kworm,B. mori on fat body glycogen, protein, tota1 lipids, phospholipids, neutral lipids and haemolymph trehalose and protein has been studied. The fat body glycogen increased significantly in all the treated groups except in the 15$\mu$g/ml androstenedione treated group. However the fat body glyco- gen increased significantly in 10 ${\mu}g$/ml methyltesterone treated group. The androstenedione treated groups showed increased haemolymph trehalose but the increase was not significant. However the haemolymph trehalose increased significantly in all the methyltestosterone treated groups. The fat body protein decreased in all the androstenedione and methyltesterone treated groups except 15 ${\mu}g$/ml methyltesterone where the decrease in fat body protein was significant. Whereas that of haemolymph protein significantly decreased in all the groups except in the group treated with 5 ${\mu}g$/ml androstenedione where the decrease was not significant. The total lipids, phospholipids and neutral lipids of the fat body decreased significantly in all the groups treated with androstenedione and methyltestosterone when compared with those of carrier control.

• International Journal of Industrial Entomology and Biomaterials
• /
• v.3 no.2
• /
• pp.149-152
• /
• 2001

Effect of topical application with 10, 20 and 30 ${\mu}g$/ml testosterone propionate and estradiol -17${\beta}$ on the fourth and fifth instar bivoltine NB18 silkworm larvae Bombyx mori, on the glycogen and protein contents of the Fat body and trehalose and protein contents of the haemolymph has been studied. Glycogen content of the fat body was significantly decreased in both testosterone propionate and estradiol -17${\beta}$ treatment groups except in the group treated with 30 ${\mu}g$ testosterone propionate where the increase was not significant when compared with those of carrier controls. The increase/decrease in haemolymph trehalose content did not show any significant difference in all the treated groups. Protein content of the fat body significantly increased in 10 and 20 mg testosterone propionate and estradiol -l7${\beta}$ treated groups but in 30 mg treated groups the increase was not significant when compared with those of carrier controls. There was no significant change in the haemolymph protein content in all the testosterone propionate and estradiol -17${\beta}$ treated groups except in group treated with 10 ${\mu}g$ estradiol -17${\beta}$ where it showed a significant decrease when compared with that of carrier control.

• International Journal of Industrial Entomology and Biomaterials
• /
• v.2 no.2
• /
• pp.181-184
• /
• 2001

The effect of topical application with 10, 20 and 30 $\mu\textrm{g}$/ml cortisone and hydrocortisone to the fifth stadium larvae of the silkworm, B. mori on fat body glycogen, protein, total lipids, phospholipids, neutral lipids and haemolymph trehalose and protein has been studied. The fat body glycogen haemolymph trehalose significantly decreased in all the treated groups except in the 10 and 20 $\mu\textrm{g}$/ml treated groups. The fat body protein increased significantly in all the cortisone and hydrocortisone treated groups except in the group treated with 10 $\mu\textrm{g}$ hydrocortisone. Whereas that of haemolymph protein significantly increased in all the groups treated with cortisone and hydrocortisone. The total lipids, phospho1ipids and neutral lipids of the fat body decreased significantly in all the groups treated with cortisone and hydrocortisone when compared with that of carrier control.

• Korean Journal of Microbiology
• /
• v.25 no.4
• /
• pp.309-317
• /
• 1987

It was known that normal-haemolymph from the 3rd instar larvae of Lucillia illustris contain a lysozyme (or lysozyme-like substance) with bactericidal activity to fram positive bacteria, and the bactericidal activity of injured-haemolymph was increased significantly after injuring the body wall. To elucidate the defence mechanism of insect against the nonpathogenic bacteria, the immune-haemolymph against Escherichia coli K-12 was prepared after immunization. The bactericidal activity between injured and immune-haemolymph was compared, and it was revealed that the immune-haemolymph showed higher titer of bactericidal activity to fram positive bacteria as well as to Escherichia coli. The bactericidal substance from the immune-haemolymph was purified through a successive chromatographies on Sephacryl S-300 and CM-Sepharose CL-6B, and it was characterized as a basic protein in nature with heat stable property at acidic conditions.

• International Journal of Industrial Entomology and Biomaterials
• /
• v.9 no.1
• /
• pp.131-135
• /
• 2004

Oral supplementation with potassium permanganate (30, 50 and 100 $\mu\textrm{g}$) to fifth instar larvae of the ${CSR_2}{\times}{CSR_4}$ race of the silkworm, B. mori resulted in a significant increase in the glycogen content of the fat body and haemolymph trehalose. The protein content of the fat body is also significantly increased in all the potassium permanganate treated groups where as that of the haemolymph is significantly increased only in the 30 ${\mu}g4 fed group. The total lipids content of the fat body increased significantly in all the potassium permanganate treated groups. This indicates that the potassium permanganate may stimulate metabolic activity, there by influencing the biochemical contents in the fat body and haemolymph of the silkworm, B. mori.

• Journal of Sericultural and Entomological Science
• /
• v.36 no.2
• /
• pp.119-123
• /
• 1994

By in vivo labelling of AMHP using[35S]-methionine, fat body culture and immunological analysis, it is proved that Bombyx adult fat body synthesizes 18K and 20K subunits of AMHP and releases them into haemolymph. Also these peptides are assembled to form native AMHP in the adult haemolymph.