• Title/Summary/Keyword: Haemolymph

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Anti-CHH Antibody Causes Impaired Hyperglycemia in Penaeus monodon

  • Treerattrakool, Supattra;Udomkit, Apinunt;Panyim, Sakol
    • BMB Reports
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    • v.39 no.4
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    • pp.371-376
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    • 2006

  • Crustacean hyperglycemic hormone (CHH) plays a major role in controlling glucose level in the haemolymph and also triggers important events during molting and reproductive cycles. In Penaeus monodon, three types of CHH, namely Pem-CHH1, Pem-CHH2 and Pem-CHH3, have been previously characterized. In this study, mouse polyclonal antibody was raised against recombinant Pem-CHH1 that was expressed in Escherichia coli. The anti-Pem-CHH1 antibody recognized all three types of Pem-CHHs but did not cross-react with either related hormone, molt-inhibiting hormone of P. monodon, or unrelated human growth hormone. The hyperglycemic activity in the extract from the eyestalk neural tissues was significantly depleted after incubating with anti-Pem-CHH antibody. Direct injection of the antibody into shrimp caused about 30-50% reduction in the haemolymph glucose level. The result demonstrates the ability of anti-Pem-CHH1 antibody to deplete the activity of CHH in vivo, and thus provides a possibility of using anti-Pem-CHH1 antibody to inhibit the hormone activity as a strategy to modulate growth and reproduction in this species.

  • https://doi.org/10.5483/BMBRep.2006.39.4.371 인용 PDF

Purification and Characterization of Apolipophorin-III from Haemolvmph of Fall Webworm Hvphantria cunea Drury (미국흰불나방(Hyphantria cunea Drury) 혈림프부터 apolipophorin-III의 순수정제 및 특성)

  • 윤화경;서신자김학열
    • The Korean Journal of Zoology
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    • v.37 no.4
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    • pp.488-494
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    • 1994

  • Apolipophorin-III (ApoLp-III) was purified from adult haemolynph of Hyphantriu cuneo and their molecular weight and synthetic place were investigated. ApoLp-III purification was performed by KBr-density gradient ultracentrifugation followed by gel permeation chromatographv (Sephadex G-1001 and ion-exchange chromatography (CM-52) and their purity was confirmed on 10% SDS-PAGE. ApoLp-III has the molecular weight of 18 ItDa and is synthesized by fat body.

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The Effects of Vitamin C on Biological, Biochemical and Economical Characteristics of the Silkworm, Bombyx mori L.

  • Etebari, Kayvan;Ebadi, Rahim;Matindoost, Leila
    • International Journal of Industrial Entomology and Biomaterials
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    • v.8 no.1
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    • pp.81-87
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    • 2004

  • In order to investigate the effects of supplementary nutrients on silkworms, Bombyx mori, an experiment was conducted with ascorbic acid treatments. Dietary supplements of ascorbic acid 1, 2 and 3% were fed to silkworm larvae through 1st to 5th instar, The larvae were fed by mulberry loaves of Kokoso variety and the supplementary loaves were used once a day. These treatments resulted in a significant increase of biological parameters such as larval weight, the rate of food consumption and the approximate digestibility of the food. But the economical parameters such as cocoon weight and cocoon shell weight didnt show considerable difference compared to control. Dietary supplement of 2% ascorbic acid increased the larval weight by 7.8% and reached to 1.065g, which had the highest weight increase in the fourth day of 4th instar larvae. The percentage of daily weight increase in this group of larvae (79.01%) had significant difference compared with other treatments. The nutritional efficiency index in this group of larvae was better than others. Also the abundance of biochemical macromolecules such as glucose, cholesterol, triacylglycerol and urea in haemolymph of larvae fed by 2% ascorbic acid increased to become 29.75 (mg/㎗), 24 (mg/㎗), 75.4(mg/㎗) and 32.1(mg/㎗) respectively. But protein contents of haemolymph of larvae in each treatment were not significantly different. Since all the results achieved were not considerable either statistically or economically, this method could not be recommended to improve the sericultural parameters.

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