• 미생물학회지
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• 제25권4호
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• pp.309-317
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• 1987

정상 상태의 연두금 파리 (Lucilia illustris) 제 3령충의 체액 (normal-haemolymph)으로 부터 그람양성 세균에 대하여 항균력이 있는 lysozymec (or lysozyme-like substance)을 확인하였으며 체벽을 손상시킨 체액 (injured-haemolymph)의 경우 그 항균력이 증가되었다. 특히 비병원성 세균인 Escherichia coli K-12로 면역하여 얻은 체액 (immune-haemolymph)의 경우 injured-haemolymph보다 그람양성 세율에 대하여 더 높은 항균력을 보였을 뿐만 아니라 그람음성 세균인 E. coli에 대하여 도 항균력을 나타내었다. Immune-haemolymph로 부터 유효 분획을 얻고 이에 대한 성상을 분석하기 위하여 Sephacryl S-300 및 CM-Sepharose CL-6B로 분리시킨 결과 상기 항균울질은 엽기성 만백질로서 산성하에서 내열성의 성질을 나타냈다.

• 한국동물학회지
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• 제23권1호
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• pp.1-12
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• 1980

큐티클 形成 및 硬化過程中 血蛋白質의 變化와 起源을 규명하고자 acrylamide gel electrophoresis와 immunodiffusion 方法을 使用하였다. Acrylamide gel electrophoresis에서 적어도 19개의 protein band가 혈림프에서 發見되었으며 脂肪體에서는 13개의 band가 確認되었는데 이들은 대체적으로 일정한 pattern을 유지하였다. 또한 혈림프와 脂肪體의 一般的인 protein band의 pattern은 $3\\sim4$개의 강하게 染色된 band와 몇 개의 가는 band가 gel의 상단에 存在하는 것이 특징이었고 적어도 5개 이상의 haemolymph protein band가 　期初에 걸쳐 일정하게 나타났다. Immunodiffusion test에서는 $8\\sim9$개의 血蛋白質에 　期初에서 나타났는데 그중 2개의 血蛋白質은 　期前에 나타났으며 다른 두 血蛋白質도 　化직 후 脂肪體에서 나타남으로써 脂肪體가 이들 血蛋白質의 起源임을 암시하였다.

• International Journal of Industrial Entomology and Biomaterials
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• 제12권2호
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• pp.75-80
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• 2006

Changes in the levels of protein and free amino acids in the haemolymph of three selected races of the silkworm, Bombyx mori viz., PM, $NB_4D_2$ and $CSR_2$, were investigated during 4th moult, 5th instar and pupal period. The levels of total protein in the haemolymph, increased from first day of 5th instar till sixth day. From seventh day till spinning, the protein levels decreased in all the three races. A sustained decrease in the haemolymph proteins was observed during the pupal development in all the three races. The levels of free amino acids, which were high during 4th moult, declined through the 5th age of larval development till spinning. PM showed a relatively higher free amino acid level (3.192 mg/ml) in haemolymph followed by $NB_4D_2$ (2.601 mg/ml) and $CSR_2$ (2.35 mg/ml). The free amino acid levels decreased gradually from prepupal stage but increased again at the end of pupal period. Racial differences in the changes in the levels of protein and free amino acids in the haemolymph were observed in the larvae and pupae when subjected to two high temperature regimes of $30^{\circ}C$ and $35^{\circ}C$. The results showed that high temperature induces specific changes in the metabolism (reversible thermal stress) that have different adaptive value in different races of the silkworm. Relatively higher increase in the free amino acid levels in the haemolymph of Pure Mysore presumably provides protective cover to tissues against high temperature by an increase in osmolarity and reduction in evaporative water loss. The absence of such a mechanism may be responsible for temperature susceptibility of the bivoltine races like $NB_4D_2$ and $CSR_2$.

• International Journal of Industrial Entomology and Biomaterials
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• 제9권1호
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• pp.131-135
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• 2004

Oral supplementation with potassium permanganate (30, 50 and 100 $\mu\textrm{g}$) to fifth instar larvae of the ${CSR_2}{\times}{CSR_4}$ race of the silkworm, B. mori resulted in a significant increase in the glycogen content of the fat body and haemolymph trehalose. The protein content of the fat body is also significantly increased in all the potassium permanganate treated groups where as that of the haemolymph is significantly increased only in the 30 ${\mu}g4 fed group. The total lipids content of the fat body increased significantly in all the potassium permanganate treated groups. This indicates that the potassium permanganate may stimulate metabolic activity, there by influencing the biochemical contents in the fat body and haemolymph of the silkworm, B. mori.

• 한국잠사곤충학회지
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• 제36권2호
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• pp.119-123
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• 1994

누에의 성충체액 주단백질(AMHP)의 생리·생화학적 기능을 구명하기 위한 연구의 하나로 AMHP의 생체내 합성과정을 autoradiography, 효소 혈청학적 방법 및 지방체의 in vitro 배양실험 등에 의해 조사하였다. 그 결과 성충의 지방체는 AMHP의 subunnit인 18K및 20K의 polypeptide를 합성하여 체액중으로 방출하며, 방출된 이들은 체액중에서 서로 결합하여 천연의 AMHP를 형성하는 것으로 확인되었다.

• International Journal of Industrial Entomology and Biomaterials
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• 제14권1호
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• pp.37-44
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• 2007

A study was made of the haemolymph protein spectrum of mulberry silkworm (Bomhyx mori L.) from the first larval instar to imago. Horizontal starch gel electrophoresis was used. Sixteen races and eight F1 interracial hybrids, raised in Bulgaria, were analyzed. During the ontogenesis, a total of 17 protein bands (15 cathodic and 2 anodic) were detected. Distinct dynamics in the haemolymph protein spectrum was observed, in result of different expression during the individual development associated with the processes of growth, histolysis and histogenesis. Based on the ontogenetic dynamics found, a correspondence was assumed between some proteins detected by us using the starch gel electrophoresis and major haemolymph proteins (SP1, SP2, MHPs and Vg) detected by other authors using the polyacrilamide gel electrophoresis. Intraracial and interracial polymorphism was observed in four protein zones. The effect of four polymorphic loci with codominant and null alleles was suggested.

• 한국응용곤충학회지
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• 제25권4호
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• pp.215-220
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• 1986

조명나방의 유충성장(幼蟲成長)에 따른 탄수화물대사(炭水化物代謝)의 변화(變化) 및 혈림프와 충체(蟲體)내 항상성(恒常性)을 구명(究明)키 위해 Glucose, Trehalose 그리고 Glycogen을 측정한 결과는 다음과 같다. 헐림프내 Glucose는 3령유충에서 최고함량(最高含量)을 나타냈으며 4령유충에서 급격히 감소(減少)하다가 종령유충으로 성장(成長)하면서 다시 증가(增加)하였고, 충체(蟲體)에서는 유충기(幼蟲期) 성장과정동안 서서히 감소(減少)하는 현상(現象) 나타냈다. Trehalose는 혈림프에서 유충기의 성장과 더불어 증가하면서 높은 함량을 나타낸 반면, 충체(蟲體)에서는 낮은 함량을 보였다. Glycogen은 혈림프에서 Trehalose와 같은 패턴으로 증가하였으나, 충체(蟲體)내에서는 Trehalose와 반대현상(反對現象)을 나타내면서 증가하였다.

• International Journal of Industrial Entomology and Biomaterials
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• 제3권1호
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• pp.89-92
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• 2001

The effect of topical application with 5,10 and 15 $\mu$g/ ml androstenedione and methyltesterone to the fifth stadium larvae of the si1kworm,B. mori on fat body glycogen, protein, tota1 lipids, phospholipids, neutral lipids and haemolymph trehalose and protein has been studied. The fat body glycogen increased significantly in all the treated groups except in the 15$\mu$g/ml androstenedione treated group. However the fat body glyco- gen increased significantly in 10 ${\mu}g$/ml methyltesterone treated group. The androstenedione treated groups showed increased haemolymph trehalose but the increase was not significant. However the haemolymph trehalose increased significantly in all the methyltestosterone treated groups. The fat body protein decreased in all the androstenedione and methyltesterone treated groups except 15 ${\mu}g$/ml methyltesterone where the decrease in fat body protein was significant. Whereas that of haemolymph protein significantly decreased in all the groups except in the group treated with 5 ${\mu}g$/ml androstenedione where the decrease was not significant. The total lipids, phospholipids and neutral lipids of the fat body decreased significantly in all the groups treated with androstenedione and methyltestosterone when compared with those of carrier control.

• 한국동물학회지
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• 제26권2호
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• pp.83-93
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• 1983

누에의 卵形成時期동안 자성 혈림프에서 DEAE-cellullose column을 使用하여 vitellogenin을 순수하게 分離해 낸 후, 電氣泳動法과 免疫學的 方法을 使用하여 각 器官에 따른 vitellogenin의 分布와 이의 量的 變化를 調査하였다. 누에의 vitellogenin은 난성숙시기에 걸쳐 혈림프, 脂肪體 및 卵巢에 모두 分布하고 있으며, 특히 포화후 7일까지만 확인되었으며 그 이후 급격한 量的 減少를 나타내었으며, 또한 血蛋白質은 卵巢蛋白質과 最小한 3개의 同質인 蛋白質을 갖고 있는 것으로 보아 卵形成에 vitellogenin 이외의 다른 血蛋白質도 관여 되는 것 같다.

• International Journal of Industrial Entomology and Biomaterials
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• 제3권2호
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• pp.149-152
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• 2001

Effect of topical application with 10, 20 and 30 ${\mu}g$/ml testosterone propionate and estradiol -17${\beta}$ on the fourth and fifth instar bivoltine NB18 silkworm larvae Bombyx mori, on the glycogen and protein contents of the Fat body and trehalose and protein contents of the haemolymph has been studied. Glycogen content of the fat body was significantly decreased in both testosterone propionate and estradiol -17${\beta}$ treatment groups except in the group treated with 30 ${\mu}g$ testosterone propionate where the increase was not significant when compared with those of carrier controls. The increase/decrease in haemolymph trehalose content did not show any significant difference in all the treated groups. Protein content of the fat body significantly increased in 10 and 20 mg testosterone propionate and estradiol -l7${\beta}$ treated groups but in 30 mg treated groups the increase was not significant when compared with those of carrier controls. There was no significant change in the haemolymph protein content in all the testosterone propionate and estradiol -17${\beta}$ treated groups except in group treated with 10 ${\mu}g$ estradiol -17${\beta}$ where it showed a significant decrease when compared with that of carrier control.