• Proceedings of the Korea Water Resources Association Conference
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• 2010.05a
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• pp.664-668
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• 2010

유역 내 효율적 수자원관리 및 이 치수 계획수립을 위해 수계별 혹은 지역별로 다양한 형태의 하천정비 사업이 시행되고 있으며, 사업의 대부분은 통수능 확보를 위한 확폭 및 홍수피해방지를 위한 제방의 보강이 주를 이루고 있다. 그러나 이러한 하천정비사업 계획의 검토를 위하여 횡방향 흐름을 고려할 수 없는 1차원 HEC-RAS 모형이 주로 이용되고 있으며, 이는 만곡부와 같은 복잡한 흐름특성이 발생하는 구간에서의 정비 사업 후 흐름특성 변화 및 하상의 안정성에 대한 평가를 위해 1차원 수치모형의 적용이 제약적임을 의미한다. 본 연구에서는 용담댐 하류부 대유리에 위치한 만곡부를 대상으로 2차원 수치모형인 CCHE2D를 이용하여 정비사업 전 후의 흐름특성 변화에 대한 분석을 수행하였다. 분석결과, 만곡부 정점부의 단면축소로 흐름의 통제가 발생하던 구간에서 정비사업 후 유속과 소류력이 감소되었으며, 상류측으로 수위가 크게 저하하였다. 또한, 정비사업 후 상류측에서 유속과 소류력의 증가가 관측되었으며, 만곡부 내측으로 유속과 소류력의 중심이 이동함에 따라 제방의 안정성에 영향을 줄 수 있음을 확인할 수 있었다. CCHE2D모형은 자연하천의 만곡부와 같은 복잡한 흐름이 발생하는 구간의 분석을 위하여 효율적으로 이용될 수 있으며, 모형 내 하상변동 모듈과의 연계모의를 통해 제방의 안정성 및 하상의 안정성 평가를 위한 수리학적 기초자료를 제공할 수 있을 것으로 판단된다.

• Proceedings of the Korea Water Resources Association Conference
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• 2008.05a
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• pp.674-679
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• 2008

수질모델링은 제한된 공간에서 실측된 자료를 바탕으로 임의지점에서의 수질을 예측하는 것으로서 수체로 유입되는 오염원의 변화에 대한 수질의 변화를 예측, 장래 개발계획 및 수질정책에 대한 영향평가 등에 활용될 수 있다. 하천 및 호소로 유입되는 오염물질을 통제하고 과학적으로 관리하기 위해서는 수질모델링 과정을 정확히 이해하고 관련모델을 적절히 적용하여야 한다. 최근 수질오염총량제가 시행되고 있는 시점에서 지자체간 갈등을 줄이고 지역의 균형발전을 위해서는 합리적이고 과학적인 수질모델링 과정이 필요하다. 따라서 본 연구에서는 금강 수계 미호천의 지류인 보강천을 대상으로 QUAL2E 하천수질모형을 구축함으로써 해당 수계에 인접한 지자체들이 원활이 수질오염총량관리제도를 시행할 수 있게 정확하고 합리적인 수질예측 결과를 제시하고자 한다. 본 연구에서는 QUAL2E 하천수질모형을 구축하기 위해서 유량자료는 2007년 10월부터 12월까지 총 8회에 걸쳐 측정한 유량 데이터를 이용하였고 수리계수는 보강천 하천정비기본계획(건설교통부, 1991)에 실려있는 하천단면 자료를 이용한 HEC-RAS 수리모형을 이용하여 산정하였다. 하천 수질 자료는 2007년 10월부터 12월까지 총 8회에 걸쳐 유량과 함께 측정한 수질자료를 이용하였고 유달계수는 S. R. HA and M. S. Bae(2003, 2004, 2005, 2006), 박정하 등(2007)이 제안한 방법을 이용하여 산정하였다. 분석 결과, 보강천에 영향을 미치는 지자체는 증평군, 진천군, 괴산군, 음성군이며 그 중 유역면적을 많이 차지하고 있는 증평군과 진천군의 영향이 큰 것으로 나타났고, 미호천 하류에 위치한 미호B 목표수질관리지점의 목표수질인 4.3 mg/L BOD를 만족하는 것으로 분석되었다.

• Journal of the Korean Society of Hazard Mitigation
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• v.7 no.5
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• pp.179-186
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• 2007

This paper has investigated a rise of water level in upstream and downstream of bridge, which is caused by accumulation of debris in a bridge. The debris has been classified into several types in terms of size. The rise of water level which has been caused by installation and removal of sheet pile that is used to block water in a bridge has been analyzed using HEC-RAS model. According to the analysis, it has turned out that the debris has no influence on the rise of water level in ordinary water flow. In addition, sheet pile has little impact on the rise of water as well. Even though the impact of sheet file has turned out trivial in flood flow just like the ordinary water flow, it's been simulated that the maximum water level difference between upstream and downstream of bridge turned out more than 1.0meter because of debris in 80-year or more flood frequency. When the rise of water level in upstream from the cross section of the bridge was investigated based on 100-year flood frequency, besides, it has turned out that it had an influence up to 17.84km distance because of the effect of debris.

• Archives of Pharmacal Research
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• v.29 no.3
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• pp.224-234
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• 2006

We employed human SK-MEL-28 cells as a model system to identify cellular proteins that accompany N-(4-methyl)phenyl-O-(4-methoxy)phenyl-thionocarbamate (MMTC)-induced apoptosis based on a proteomic approach. Cell viability tests revealed that SK-MEL-28 skin cancer cells underwent more cell death than normal HaCaT cells in a dose-dependent manner after treatment with MMTC. Two-dimensional electrophoresis in conjunction with matrixassisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry analysis or computer matching with a protein database further revealed that the MMTC-induced apoptosis is accompanied by increased levels of caspase-1, checkpoint suppressor-1, caspase-4, NF-kB inhibitor, AP-2, c-Jun-N-terminal kinase, melanoma inhibitor, granzyme K, G1/S specific cyclin D3, cystein rich protein, Ras-related protein Rab-37 or Ras-related protein Rab-13, and reduced levels of EMS (oncogene), ATP synthase, tyrosine-phosphatase, Cdc25c, 14-3-3 protein or specific structure of nuclear receptor. The migration suppressing effect of MMTC on SK-MEL-28 cell was tested. MMTC suppressed the metastasis of SK-MEL-8 cells. It was also identified that MMTC had little angiogenic effect because it did not suppress the proliferation of HUVEC cell line. These results suggest that MMTC is a novel chemotherapeutic and metastatic agents against the SK-MEL-28 human melanoma cell line.

• Journal of Biomedical Engineering Research
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• v.40 no.3
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• pp.105-115
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• 2019

Brain tumor surgery may be difficult, but it is also incredibly important. The technological improvements for traditional brain tumor surgeries have always been a focus to improve the precision of surgery and release the potential of the technology in this important area of the body. The need for precision during brain tumor surgery has led to an increase in Robotic-assisted surgeries (RAS). One of the challenges to the widespread acceptance of RAS in the neurosurgery is to recognize invisible tumor accurately. Therefore, it is important to detect brain tumor size and location because surgeon tries to remove as much tumor as possible. In this paper, we proposed brain tumor detection procedures for MRI (Magnetic Resonance Imaging) system. A method of automatic brain tumor detection is needed to accurately target the location of the lesion during brain tumor surgery and to report the location and size of the lesion. In the qualitative assessment, the proposed method showed better results than those obtained with other brain tumor detection methods. Comparisons among all assessment criteria indicated that the proposed method was significantly superior to the threshold method with respect to all assessment criteria. The proposed method was effective for detecting brain tumor.

• Journal of Acupuncture Research
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• v.18 no.4
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• pp.101-115
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• 2001

Objective : To characterize the antitumorigenic potential of Apamin, one of the major components of bee venom, its effects on cell proliferation and the mitogen-activated protein kinase (MAPK) signal transduction pathway were characterized using the human melanoma cell line SK-MEL-2. Methods & Results : Cell counting analysis for cell death demonstrated that consistent with a previous results, SK-MEL-2 cells treated with $0.5-2.0{\mu}g/ml$ of Apamin showed no recognizable cytotoxic effect whereas detectable induction of cell death was identified at concentrations over $5.0{\mu}g/ml$. [3H]thymidine incorporation assay for cell proliferation demonstrated that DNA replication of SK-MEL-2 cells is inhibited by Apamin in a dose- and time-dependent manner. To explore whether Apamin-induced growth suppression is associated with the MAPK signaling pathway, phosphorylation of Erk, a function mediator of MAPK growth-stimulating signal, was examined Western blot assay using a phospho-specific Erkl/2 antibody. A significant increase of Erkl/2 phosphorylation level was observed in Apamin-treated cells compared with untreated control cells. Qantitative RT-PCR analysis revealed that Apamin inhibit expression of MAPK downstream genes such as c-Jun, c-Fos, and cyclin D1 but not expression of MAPK pathway component genes including Ha-Ras, c-Raf-1, MEK1, and Erk. Conclusion : It is strongly suggested that the antitumorigenic activity of Apamin might result in part from its inhibitory effect on the MAPK signaling pathway in human melanoma cells SK-MEL-2.

• Biomolecules & Therapeutics
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• v.18 no.4
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• pp.386-395
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• 2010

Bone marrow-derived mesenchymal stem cells (BM-MSC) are a multipotent cell population that can differentiate into neuron-like cells. Previously it has been reported that murine BM-MSC can differentiate into neuron-like cells by co-treatment with a Rho-associated kinase (ROCK) inhibitor -Y27632 and $CoCl_2$. In this study, we compared several ROCK inhibitors for the ability to induce human BM-MSCs to differentiate into neuron-like cells in the presence of $CoCl_2$. Y27632 with high specificity for ROCK at 1-30 ${\mu}M$ was best at inducing neuronal differentiation of MSCs. Compared to HA1077 and H1152, which also effectively induced morphological change into neuron-like cells, Y27632 showed less toxicity even at 100 ${\mu}M$, and resulted in longer multiple branching processes at a wide range of concentrations at 6 h and 72 h post-induction. H89, however, which has less specificity by inhibition of protein kinase A, S6 kinase 1 and MSK1 with similar or greater potency, was less effective at inducing neuronal differentiation of MSCs. Simvastatin, which can inhibit Rho, Ras, and Rac by blocking the synthesis of isoprenoid intermediates, showed little activity for inducing morphological changes of MSCs into neuron-like cells. Accordingly, the expression patterns for neuronal cell markers,including ${\beta}$-tubulin III, neuron-specific enolase, neurofilament, and microtubule-associated protein, were consistent with the pattern of the morphological changes. The data suggest that the ROCK inhibitors with higher specificity are more effective at inducing neuronal differentiation of MSCs.

• Parasites, Hosts and Diseases
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• v.53 no.4
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• pp.421-430
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• 2015

The parasite Plasmodium falciparum causes severe malaria and is the most dangerous to humans. However, it exhibits resistance to their drugs. Farnesyltransferase has been identified in pathogenic protozoa of the genera Plasmodium and the target of farnesyltransferase includes Ras family. Therefore, the inhibition of farnesyltransferase has been suggested as a new strategy for the treatment of malaria. However, the exact functional mechanism of this agent is still unknown. In addition, the effect of farnesyltransferase inhibitor (FTIs) on mitochondrial level of malaria parasites is not fully understood. In this study, therefore, the effect of a FTI R115777 on the function of mitochondria of P. falciparum was investigated experimentally. As a result, FTI R115777 was found to suppress the infection rate of malaria parasites under in vitro condition. It also reduces the copy number of mtDNA-encoded cytochrome c oxidase III. In addition, the mitochondrial membrane potential (${\Delta}{\Psi}m$) and the green fluorescence intensity of MitoTracker were decreased by FTI R115777. Chloroquine and atovaquone were measured by the mtDNA copy number as mitochondrial non-specific or specific inhibitor, respectively. Chloroquine did not affect the copy number of mtDNA-encoded cytochrome c oxidase III, while atovaquone induced to change the mtDNA copy number. These results suggest that FTI R115777 has strong influence on the mitochondrial function of P. falciparum. It may have therapeutic potential for malaria by targeting the mitochondria of parasites.

• Journal of Wetlands Research
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• v.12 no.1
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• pp.33-40
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• 2010

In this study, it has been intended to present the ways to improve some problems such as the difficulty of using the program which had got from the existing study, the computation and application of a lot of parameter and the complicated processing which need to be more simplified. Also It has been tried to bring up the ways to make a flood inundation map and a detailed inundation analysis which could reduce the risk factors. We selected an Anseong-Cheon basin, and wrote a flood inundation scenario based on extreme flood to exceed the planned frequency to consider only overflow and levee break and executed inundation simulation. Researchers conducted an analysis of overflow and levee break using function of HEC-RAS Storage with a One-Dimensional model. It applied Elevation versus Volume Curve for more correct inundation simulation than a method of Area-Time-Depth which used in popular. This study will suggest a mathematical method of SURFER with a little difference of inundation area more simplified and precise flood inundation than complicated Arcview 3.2a which used Query method of Arcview 3.2a.

• Development and Reproduction
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• v.28 no.1
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• pp.13-19
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• 2024

Ras-related (Rab) proteins, integral members of the monomeric G-protein family, play a pivotal role in regulating intracellular vesicular transport. These proteins contribute to male reproductive processes, specifically in acrosome formation, exocytosis, and sperm motility. Although a prior study indicated a correlation between Rab3A and sperm motility, including motion kinematic parameters such as mean dance, this association has only been explored within a limited sample size. Therefore, further verification is required to confirm the correlation between Rab3A and sperm motility parameters. In the present study, Rab3A expression, sperm motility, and motion kinematic parameters were analyzed in 150 boar spermatozoa. Additionally, correlations between Rab3A expression and sperm kinematic characteristics were evaluated statistically. The results revealed significant associations between Rab3A protein expression levels and various motion kinematic parameters. Specifically, Rab3A levels exhibited positive correlations with average path velocity (p<0.05), mean amplitude of lateral head displacement (p<0.05), and curvilinear velocity (p<0.01). Consequently, it is proposed that Rab3A protein plays a crucial role in male fertility through its correlation with sperm kinematic characteristics, making it a potential marker for sperm motility-related assessments.