• Structural Engineering and Mechanics
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• 제50권2호
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• pp.201-214
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• 2014

Due to the confinement effects, Steel-Straps Tensioning Technique (SSTT) can significantly enhance the strength and ductility of high-strength concrete (HSC) members (Moghaddam et al. 2008). However, the enhancement especially in strength may result in slender member and more susceptible to instability (Jiang and Teng 2012a). This instability is particularly significant in HSC member as it inherent the brittle nature of the material (Galano et al. 2008). The current slenderness limit expression used in the design is mainly derived from the experiment and analysis results based on Normal strength concrete (NSC) column and therefore the direct application of these slenderness limit expressions to the HSC column is being questioned. Besides, a particular slenderness limit for the SSTT-confined HSC column which incorporated the pre-tensioned force and multilayers effects is not yet available. Hence, an analytical study was carried out in the view of developing a simple equation in order to determine the slenderness limit for HSC column confined with SSTT. Based on the analytical results, it was concluded that the existing slenderness limit expressions used in the design are appropriate for neither HSC columns nor SSTT-confined HSC columns. In this paper, a slenderness limit expression which has incorporated the SSTT-confinement effects is proposed. The proposed expression can also be applied to unconfined HSC columns.

• 한국자기공명학회논문지
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• 제28권2호
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• pp.6-9
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• 2024

HscA is a Hsp70-type chaperone protein that plays an essential role to mediate the iron-sulfur (Fe-S) cluster biogenesis mechanism in Escherichia coli. Like other Hsp70 chaperones, HscA is composed of two domains: the nucleotide binding domain (NBD), which can hydrolyze ATP and use its chemical energy to facilitate the Fe-S cluster transfer process, and the substrate binding domain (SBD), which directly interacts with the substrate, IscU, the scaffold protein of an Fe-S cluster. In the present work, we prepared the isolated SBD construct of HscA (HscA(SBD)) and conducted the solution-state nuclear magnetic resonance (NMR) experiments to have its backbone chemical shift assignment information. Due to low spectral quality of HscA(SBD), we obtained all the NMR data from the sample containing the peptide LPPVKIHC, the HscA-interaction motif of IscU, from which the chemical shift assignment could be done successfully. We expect that this information provides an important basis to execute detailed structural characterization of HscA and appreciate its interaction with IscU.

• Archives of Pharmacal Research
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• 제27권4호
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• pp.422-428
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• 2004

Expressed sequence tags (ESTs) were generated from two 3'-directed CDNA libraries constructed from quiescent and activated rat hepatic stellate cell (HSC) to analyze the expression profiles of active genes in both cells. From quiescent and activated HSC, 694 ESTs and 779 ESTs, respectively, were obtained after excluding those having shorter than 30 bp. Amonq ESTs obtained from quiescent and activated HSC, 68 and 73 kinds of ESTs (186 clones and 236 clones), respectively, appeared more than once, implying that their genes are expressed highly in each cell type. 52 among 73 ESTs appeared only in the activated HSC 47 amonq 68 ESTs only in the normal HSC, and 21 in both cells. The genes of these 52 ESTs were assumed to be expressed more highly in the activated HSC. To confirm the high expression of genes of which the ESTs appeared more than twice in the activated HSC, northern hybridization was carried out with RNAs derived from rat normal and fibrotic liver using each of 18 EST DNAs as probe. 13 ESTs showed more intense bands with RNA isolated from the fibrotic liver than normal liver. From these results, we confirm the positive correlation between abundance of transcript in activated HSCs and the expression level in fibrotic liver, The expression profile of the transcripts serves as an important tool in understanding the biological properties of HSC.

• 한국화재소방학회논문지
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• 제22권4호
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• pp.85-94
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• 2008

본 연구는 HSC의 폭렬제어 및 내화성능 확보 방안 중 하나인, HSC에 내화성능을 갖는 피복층을 형성하는 방안에 대하여, 피복층을 ECC로 이용하는 경우 이에 대한 화재성상 및 내화특성을 실험적으로 검토하고, 수열온도 예측 등과 같은 내화설계를 위한 기초자료를 제시하기 위한 것이다. 이를 위하여 HSC 부재에 대한 내화시험을 실시하였다. 실험변수는 ECC의 피복층 두께(20, 30, 40 mm), 시공방식(라이닝, 보수)으로 하였으며, 비교 및 검증을 위하여 피복층이 없는 HSC 및 FRCC 2종류의 충전두께의 변화에 따른 실험을 실시하였다. 도입 화재하중은 ISO 834 기준 3시간 가열곡선으로 하였으며, 각 깊이별 수열온도, 폭렬 및 균열성상, 중성화깊이를 측정 평가하였다. 실험결과 ECC는 HSC 보다 높은 차열성능을 가지고 있으며, 폭렬저감성능을 확인 할 수 있었다. 또한 회귀분석을 통하여 ECC를 HSC의 피복층으로 사용하는 경우에 대한 수열온도 간편 예측식을 제시하였으며, 이에 대한 검증을 실험결과를 통해 수행하였고 HSC를 이용한 부재에 대한 본 예측식의 적용 방법을 제시하였다.

• International Journal of Oral Biology
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• 제48권1호
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• pp.1-7
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• 2023

Oral squamous cell carcinoma (OSCC), which accounts for approximately 90% of oral cancers, has a high rate of local recurrence and a poor prognosis despite improvements in treatment. Exosomes released from OSCC cells promote cell proliferation and metastasis. Although it is clear that the biogenesis of exosomes is mediated by the endosomal sorting complex required for transport (ESCRT) machinery, the gene expression pattern of ESCRT, depending on the cell type, remains elusive. The exosomal release from the human OSCC cell lines, HSC-3 and HSC-4, and their corresponding gefitinib-resistant sub-cell lines, HSC-3/GR and HSC-4/GR, was assessed by western blot and flow cytometry. The levels of ESCRT machinery proteins, including Hrs, Tsg101, and Alix, and whole-cell ubiquitination were evaluated by western blot. We observed that the basal level of exosomal release was higher in HSC-3/GR and HSC-4/GR cells than in HSC-3 and HSC-4 cells, respectively. Long-term gefitinib exposure of each cell line and its corresponding gefitinib-resistant sub-cell line differentially induced the expression of the ESCRT machinery. Furthermore, whole-cell ubiquitination and autophagic flux were shown to be increased in gefitinib-treated HSC-3 and HSC-4 cells. Our data indicate that the expression patterns of the ESCRT machinery genes are differentially regulated by the characteristics of cells, such as intracellular energy metabolism. Therefore, the expression patterns of the ESCRT machinery should be considered as a key factor to improve the treatment strategy for OSCC.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2000년도 봄 학술발표회 논문집
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• pp.47-52
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• 2000

Recently, it is very necessary the development of the manufacturing techniques for high strength concrete(HSC) for the large-scale size and good quality of civil structure. But, the manufacture and quality control of HSC of which shrinkage, heat of hydration and workability at construction filed are considered, is very difficult due to its low water-cement ratio and high quantity of unit cement content. In the present study, we tried to know and assess the influences of chemical and physical properties of cement on the material properties of HSC. We analyzed basic properties of 4 kinds of cement whose chemical and physical properties are different each other through various tests such as chemical analysis and mortal test. Also, we performed the assessment of the material properties of HSC for each dement by the test for the conditions of same mix design and similar compressive strength. From the results in the study, the assessment of the important quality factors of cement influencing the properties of HSC may be utilized to quality control of applied cement to manufacture the HSC of high quality.

• 한국지진공학회논문집
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• 제25권5호
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• pp.213-221
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• 2021

Many Korean domestic masonry structures constructed since 1970 have been found to be vulnerable to earthquakes because they lack efficient lateral force resistance. Many studies have shown that the brick and mortar suddenly experience brittle fracture and out-of-plane collapse when they reach the inelastic range. This study evaluated the seismic retrofitting of non-reinforced masonry with Hybrid Super Coating (HSC) and Cast, manufactured using glass fiber. Four types of specimen original specimen (BR-OR), one layered HSC (BR-HS-O), two-layered HSC (BR-HS-B), one layered HSC, and Cast (BR-CT-HS-O) were constructed and analyzed using compression, flexural tensile, diagonal compression, and triplet tests. The specimen responses were presented and discussed in load-displacement curves, maximum strength, and crack propagation. The compressive strength of the retrofit specimens slightly increased, while the flexural tensile strength of the retrofit specimens increased significantly. In addition, the HSC and Cast also produced a considerable increase in the ductile response of specimens before failure. Diagonal compression test results showed that HSC delayed brittle cracks between the mortar and bricks and resulted in larger displacement before failure than the original brick. The triplet test results confirmed that the bonding strength of the retrofit specimens also increased. The application of HSC and Cast was found to restrain the occurrence of brittle failure effectively and delayed the collapse of masonry wall structures.

• 대한본초학회지
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• 제24권3호
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• pp.129-138
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• 2009

Objectives : The Objective of this study is to compare the anti-oxidative effect of the Hoveniae Semen cum Fructus (HScF) to that of Hoveniae Ramulus (HR) in vivo. Methods : The extracts of HScF and HR were tested in vivo for their anti-oxidative effects. SD Rats were injected with the AAPH (2.2'-azobis (2-aminodinopropane) hydrochloride) to induce the acute oxidization stress, and the oxidized rats were administered with the HScF and HR decoction orally. The RBC, WBC and PLT populations in plasma, and the blood chemistry levels of the rats were measured to observe the overall changes in the blood. The anti-oxidative effects of the HScF and HR decoctions were investigated on the liver tissues of the oxidated rats. Results : 1. Thrombocyte decreased significantly in the group medicated with HScF. 2. Blood glucose increased significantly in the group medicated with HR. 3. SOD activity increased significantly in both the group medicated with HScF and that with HR. 4. NO concentration decreased significantly in both the group medicated with HScF and that with HR. 5. The catalase content increased significantly in both the group medicated with HScF and that with HR. Conclusions : The HScF and HR extracts have an anti-oxidative effect.

• Structural Engineering and Mechanics
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• 제48권6호
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• pp.833-848
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• 2013

Nine rectangular-section of High Strength Concrete(HSC) beams were designed and casted based on the American Concrete Institute (ACI) code provisons with varying of tensile reinforcement ratio as (${\rho}_{min}$, $0.2_{{\rho}b}$, $0.3_{{\rho}b}$, $0.4_{{\rho}b}$, $0.5_{{\rho}b}$, $0.75_{{\rho}b}$, $0.85_{{\rho}b}$, $_{{\rho}b}$, $1.2_{{\rho}b}$). Steel and concrete strains and deflections were measured at different points of the beam's length for every incremental load up to failure. The ductility ratios were calculated and the moment-curvature and load-deflection curves were drawn. The results showed that the ductility ratio reduced to less than 2 when the tensile reinforcement ratio increased to $0.5_{{\rho}b}$. Comparison of the theoretical ductility coefficient from CSA94, NZS95 and ACI with the experimental ones shows that the three mentioned codes exhibit conservative values for low reinforced HSC beams. For over-reinforced HSC beams, only the CSA94 provision is more valid. ACI bending provision is 10 percent conservative for assessing of ultimate bending moment in low-reinforced HSC section while its results are valid for over-reinforced HSC sections. The ACI code provision is non-conservative for the modulus of rupture and needs to be reviewed.

• Advances in concrete construction
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• 제11권3호
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• pp.239-253
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• 2021

The behavior of concrete containing waste glass as a replacement of cement or aggregate was studied previously in the most of researches, but the present investigation focuses on the recycling of waste glass powder as a substitute for silica fume in high strength concrete (HSC). This endeavor deals with the efficiency of using waste glass powder, as an alternative for silica fume, in the flexural capacity of HSC beam. Thirteen members with dimensions of 0.3 m width, 0.15 m depth and 0.9 m span length were utilized in this work. A comparison study was performed considering HSC members and hybrid beams fabricated by HSC and conventional normal concrete (CC). In addition to the experiments on the influence of glass powder on flexural behavior, numerical analysis was implemented using nonlinear finite element approach to simulate the structural performance of the beams. Same constitutive relationships were selected to model the behavior of HSC with waste glass powder or silica fume to show the matching between the modeling outputs for beams made with these powders. The results showed that the loading capacity and ductility index of the HSC beams with waste glass powder demonstrated enhancing ultimate load and ductility compared with those of HSC specimens with silica fume. The study deduced that the recycled waste glass powder is a good alternative to the pozzolanic powder of silica fume.