• 한국산학기술학회논문지
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• 제17권4호
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• pp.1-12
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• 2016

최적화 분야에서 Harmony Search Algorithm (HSA)은 근래에 개발된 메타휴리스틱 최적화 알고리즘의 하나로, HSA가 개발된 이래 공학, 자연과학, 의약학 등 다양한 분야에서 많은 연구자들에 의해 활용되어왔다. 현재 최적화 대상 문제들의 복잡성이 날로 증가하고 있으며 이에 따라 기존 최적화 기법에 대한 효율을 개선하는 방법론 개발에 대한 필요성이 대두되고 있다. 따라서 본 연구에서는 HSA의 구조적 특성에 초점을 맞추어 해탐색 능력을 향상시키는 것을 목표로 하여 새로운 메타휴리스틱 최적화 알고리즘인 Multi-layered Harmony Search Algorithm (MLHSA)을 제안하였다. 개발된 MLHSA는 기존 HSA에 추가적으로 구조적인 특성을 부여하여 전역 탐색 및 지역 탐색 성능을 개선하였다. 또한, 제안된 기법의 효율성과 적용성을 검증하기 위해 이전 최적화 알고리즘 관련 문헌에서 다양한 알고리즘이 적용된 바 있는 수학적 최적해 탐색 문제와 상수도 관망의 최적 관경 설계에 MLHSA를 통한 최적화를 수행하였다. 적용 결과 본 연구에서 개발된 MLHSA는 기존 알고리즘을 효과적으로 대체할 수 있는 최적화 기법임을 확인할 수 있었다.

• 한국강구조학회 논문집
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• 제29권1호
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• pp.37-48
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• 2017

본 연구에서는 HSA800 60mm 강재의 SAW 용접성 및 이음성능을 확인하기 위해 후열처리 1시간과 2시간에 대한 SAW 공시체를 제작하여 9종의 시험을 실시하였다. 용접부 충격시험, 굽힘시험, 경도시험, 매크로조직시험, 마이크로조직시험 결과 SAW PH1 및 PH2는 양호한 용접부로 나타났으나, 화학성분시험 결과 P성분이 KS를 만족하지 못하였다. 용접부 평판 및 봉형 인장시험결과 SAW PH1에 비하여 PH2의 기계적 특성이 양호하게 나타났기 때문에 SAW의 경우 후열처리는 2시간을 권장한다.

• 제어로봇시스템학회논문지
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• 제17권8호
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• pp.747-752
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• 2011

HMMs (Hidden Markov Models) are widely used for biological signal, such as EEG (electroencephalogram) sequence, analysis because of their ability to incorporate sequential information in their structure. A recent trends of research are going after the biological interpretable HMMs, and we need to control the complexity of the HMM so that it has good generalization performance. So, an automatic means of optimizing the structure of HMMs would be highly desirable. In this paper, we described a procedure of classification of motor imagery EEG signals using HMM. The motor imagery related EEG signals recorded from subjects performing left, right hand and foots motor imagery. And the proposed a method that was focus on the validation of the HSA (Harmony Search Algorithm) based optimization for HMM. Harmony search algorithm is sufficiently adaptable to allow incorporation of other techniques. A HMM training strategy using HSA is proposed, and it is tested on finding optimized structure for the pattern recognition of EEG sequence. The proposed HSA-HMM can performs global searching without initial parameter setting, local optima, and solution divergence.

• Bulletin of the Korean Chemical Society
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• 제35권9호
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• pp.2621-2624
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• 2014

The rapid and spontaneous adsorption of proteins on nanoparticle (NP) surfaces in biological fluids such as blood is an important phenomenon as it possibly determines "what the cells see" and, thus, the fates of NPs in living organisms. In order to quantitatively understand protein coronas at the molecular level, we investigated human serum albumin (HSA) coronas that were produced on silica NPs of 20 nm and 50 nm diameters using conventional gel electrophoresis. Analysis of the concentration dependence of protein adsorption showed that HSA coronas preferentially formed a monolayer on silica NPs and revealed the presence of hard protein coronas. HSA adsorption was clearly dependent on NP size, and this might be due to the different surface curvatures of NPs of different sizes.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1994년도 춘계학술대회 and 제3회 신약개발 연구발표회
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• pp.343-343
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• 1994

1. 목 적 : 혈액 중에 존재하는 약물은 대부분 혈장 단백질과 결합하며, 비단백 결합성 약물만이 생체막을 통과하여 여러 조직에 분포되고, target eel1에서 약리학적 작용을 나타내며, 대사, 배설 될 수 있다. 단백결합율이 높은 약물일수록 비결합성 약물의 양은 적어지며, 따라서 비결합성 약물의 증가는 약효의 상승을 의미하게 된다. 최근 만성 질환에 한약의 병용투여가 증가하고 있다. 본 실험에서는 단백결합율이 높은 감초의 주성분인 Glycyrrhizic acid(GA)와 고혈압 치료제로 많이 사용되는 칼슘 길항제를 병용 투여할 경우, 칼슘 길항제의 혈장 단백결합에 미치는 영향을 살펴 보았다. 2. 방 법 : Diltiazem hydrochloride, Verapamil hydrochloride, Nifedipine 와 GA를 model 약물로 하여 평형 투석법과 한외 여과법을 이용하여 fatty acid free human serum albumin(HSA), Low density lipoprotein( LDL ), of-Acid glycoprotein(AAG), plasma 각각에 대한 결합율을 HPLC로 분석하였으며 또한 Scatchard plot를 이용하여 binding parameter를 구하였다. 3. 결과 및 고찰 : GA는 Diltiazem의 HSA와 plasma의 결합율에 영향을 미쳤으며, Verapamil의 HSA, LDL, AAG, Plasma 결합율에, 그리고 Nifedipine의 HSA, LDL, Plasma의 단백 결합율에 영향을 주었으며, 각각 n과 Ka값에 변화를 주었다.

• 한국미생물·생명공학회지
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• 제22권1호
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• pp.65-72
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• 1994

Purification and characterization of pectate lyase from Bacillus sp HSA-925. Bacillus sp. HSA-925 isolated from soil produced constitutively an extracellular pectate lyase when cultivated in LB broth. The pectate lyase(EC 4.2.2.2) was purified from the cuylture broth by preciptation with ammonium sulfate, followed by column chromatography on CM-cellulose C-50 and repeated gel filtration on Sephadex G-75G. The enzyme had a molecular weight of 32-33 kDa. The activity was mazimum at pH 9.5 AND 45$\CIRC $C. The enzume activity was stable at 55$\circ $C for 15 min and between pH7-12. The activation energy, Km and V$_{max}$ for the pectate lyase were 5.8779 kcal/mol, 6.33$\times $10$^{-2}$ mol/ml and 2.09$\times $10$^{2}$ $\mu $mol/min respectively. The enzyme was activated by Ca$^{2+}$, Cu$^{2+}$ and inhibited by Li$^{+}$, Hg$^{2+}$, EDTA.

• Archives of Pharmacal Research
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• 제27권9호
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• pp.978-983
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• 2004

The effects of glycyrrhizic acid (GLZ) on protein binding of diltiazem, verapamil, and nifedipine were investigated. Protein binding studies (human serum, human serum albumin (HSA) and (X1-acid glycoprotein (AAG)) were conducted using the equilibrium dialysis method with and without addition of GLZ. The binding parameters, such as the number of moles of bound drug per mole of protein, the number of binding sites per protein molecule, and the association con-stant, were estimated using the Scatchard plot. The serum binding of nifedipine, verapamil, and diltiazem was displaced with addition of GLZ, and the decreases of Ks for serum were observed. GLZ decreased the association constants of three drugs for HSA and AAG, while the binding capacity remained similar with addition of GLZ. Although the characteristics of interaction were not clear, GLZ seemed to mainly affect HSA binding of nifedipine rather than AAG binding, while GLZ seemed to affect both AAG- and HSA-bindings of verapamil and dilt-iazem resulting in a serum binding displacement.

• Rapid Communication in Photoscience
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• 제4권2호
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• pp.41-44
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• 2015

Biomolecular photo-damaging activity of a water-soluble cationic porphyrin was examined using human serum albumin (HSA), a water-soluble protein as a target biomolecule model by a fluorometry. Dichlorophosphorus(V) tetraphenylporphyrin ($Cl_2P(V)TPP$), was synthesized and used as a photosensitizer. This porphyrin could bind to HSA and cause the photosensitized oxidation of HSA through the singlet oxygen generation and the oxidative photo-induced electron transfer (ET). Near infrared emission spectroscopy demonstrated the photosensitized singlet oxygen generation by this porphyrin. Decrement of the fluorescence lifetime of $Cl_2P(V)TPP$ by HSA supported the ET mechanism. Furthermore, the estimated Gibb's energy indicated that the ET mechanism is possible in the terms of energy. Because oxygen concentration in cancer cell is relatively low, ET mechanism is considered to be advantageous for photosensitizer of photodynamic therapy.

• 한국해양공학회지
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• 제27권5호
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• pp.36-42
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• 2013

We present the optimum design for the cross-sectional(sizing) and shape optimization of truss structures with natural frequency constraints. The optimum design method used in this paper employs continuous design variables and the Harmony Search Algorithm(HSA). HSA is a meta-heuristic search method for global optimization problems. In this paper, HSA uses the method of random number selection in an update process, along with penalty parameters, to construct the initial harmony memory in order to improve the fitness in the initial and update processes. In examples, 10-bar and 72-bar trusses are optimized for sizing, and 37-bar bridge type truss and 52-bar(like dome) for sizing and shape. Four typical truss optimization examples are employed to demonstrate the availability of HSA for finding the minimum weight optimum truss with multiple natural frequency constraints.

• 건축구조
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• 제21권6호
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• pp.47-54
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• 2014