• 한국토양비료학회지
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• 제49권4호
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• pp.393-400
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• 2016

Fusarium graminearum is the main cause of substantial economic loss in wheat production. The aim of this study is to investigate biocontrol potential of Lysobacter antibioticus HS124 against F. graminearum and to purify an antifungal compound. In preliminary study, n-butanol crude extract revealed destructive alterations in the hyphal morphology of F. graminearum and almost degraded with $1,000{\mu}g\;mL^{-1}$ concentration. For further study, the antifungal compound extracted from the n-butanol crude extract of L. antibioticus HS124 was identified as N-Butyl-tetrahydro-5-oxofuran-2-carboxamide ($C_9H_{16}NO_3$) using NMR ($^1H-NMR$, $^{13}C-NMR$, $^1H-^1H\;COSY$, HMBC, and HMQC), and HR-ESI-MS analysis. To our knowledge, N-Butyl-tetrahydro-5-oxofuran-2-carboxamide may be a novel compound with molecular weight of 186.1130. The minimum inhibitory concentration value of antifungal compound was $62.5{\mu}g\;mL^{-1}$ against F. graminearum. In an in vivo pot experiment, crown rot disease from F. graminearum was inhibited when wheat seeds were treated with both HS124 culture and F. graminearum. Growth of wheat seedling was enhanced by treatment of HS124 compared to control. Our results suggest that L. antibioticus HS124 characterized in this study could be successfully used to control F. graminearum and could be used as an alternative to chemical fungicides in modern agriculture.

• 한국융합학회논문지
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• 제6권1호
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• pp.49-55
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• 2015

FTA(Free Trade Agreement) 환경에서 수출입 기업이 무관세 혜택을 받기위해서는 원산지 규정을 준수하는 일이고, 이를 위해 가장 먼저 선행되어야하는 것이 물품의 품목분류가 필요하다. 이를 위해서는 관세율표를 구성하고 있는 HS(Harmonized System) 품목분류의 기본원리에 대한 철저한 이해가 전제되어야하며, 품목에 대한 호(Heading)에서의 상품명에 대한 이해와 관련 영역(section)이나 해당 영역(chapter)의 주(Note)는 물론 해설서의 규정까지 이해하고 있어야 올바른 품목분류를 할 수 있다. 이러한 문제점을 해결하기 위해 본 논문은 물품의 특성을 잘 알고 있는 경우, 업무 담당자도 쉽고 정확하게 분류할 수 있도록 품목분류의 결정에 영향을 줄 수 있는 7가지 결정요소를 시스템에서 먼저 제시하여 사용자가 선택하도록 하고, 각 산업에서 통상적으로 통용되는 현장용어 지식을 포함하는 융합적 수행시스템 기반의 HS 품목분류 서비스 개발을 제안하였다. 마지막으로 이 시스템의 실제 기업 현장 적용을 통해 효과를 검토하였다.

• Membrane and Water Treatment
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• 제9권3호
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• pp.137-146
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• 2018

Soil washing is one of the most frequently used remediation technologies for heavy metal-contaminated soils. Inorganic and organic acids and chelating agents that can enhance the removal of heavy metals from contaminated soils have been employed as soil washing agents. However, the toxicity, low removal efficiency and high cost of these chemicals limit their use. Given that humic substance (HS) can effectively chelate heavy metals, the development of an eco-friendly, performance-efficient and cost-effective soil washing agent using a nano-scale chelator composed of HS was examined in this study. Copper (Cu) and lead (Pb) were selected as target heavy metals. In soil washing experiments, HS concentration, pH, soil:washing solution ratio and extraction time were evaluated with regard to washing efficiency and the chelation effect. The highest removal rates by soil washing (69% for Cu and 56% for Pb) were achieved at an HS concentration of 1,000 mg/L and soil:washing solution ratio of 1:25. Washing with HS was found to be effective when the pH value was higher than 8, which can be attributed to the increased chelation effect between HS and heavy metals at the high pH range. In contrast, the washing efficiency decreased markedly in the low pH range due to HS precipitation. The chelation capacities for Cu and Pb in the aqueous phase were determined to be 0.547mmol-Cu/g-HS and 0.192mmol-Pb/g-HS, respectively.

• International Journal of Oral Biology
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• 제38권3호
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• pp.93-100
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• 2013

Bile acids and synthetic bile acid derivatives induce apoptosis in various kinds of cancer cells and thus have anticancer properties. Recently, it has been suggested that autophagy may play an important role in cancer therapy. However, few data are available regarding the role of autophagy in oral cancers and there have been no reports of autophagic cell death in OSCCs (oral squamous cell carcinoma cells) induced by HS-1200, a synthetic bile acid derivative. We thus examine whether HS-1200 modulates autophagy in OSCCs. Our findings indicate that HS-1200 has anticancer effects in OSCCs, and we observed in these cells that autophagic vacuoles were visible by monodansylcadaverine (MDC)and acridine orange staining. When we analyzed HS-1200-treated OSCC cells for the presence of biochemical markers, we observed that this treatment directly affects the conversion of LC-3II, degradation of p62/SQSTM1 and full-length beclin-1, cleavage of ATG5-12 and the activation of caspase. An autophagy inhibitor suppressed HS-1200-induced cell death in OSCCs, confirming that autophagy acts as a pro-death signal in these cells. Furthermore, HS-1200 shows anticancer activity against OSCCs via both autophagy and apoptosis. Our current findings suggest that HS-1200 may potentially contribute to oral cancer treatment and thus provide useful information for the future development of a new therapeutic agent.

• Journal of Nutrition and Health
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• 제35권1호
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• pp.78-89
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• 2002

This study was performed to analyse some rick ffactors for chronic degenerative diseases of the Korean dict. The study subjects consisted of preschool children(PC), elementary school children(EC), high school students(HS), college students(CS), and adults over 30 years old living in Seoul and the surrounding areas. The subjects over 30 years old were classfied to 30-49 years, 50-69 years, and over 70 years. A dietary survey was conducted using 24-hour recall method and data were collected from 2392 subjects. The dietary risks for chronic degenerative disease such as food sources of fat consumption, high cholesterol intake, low dietary fiber intake, and low calcium intake and high protein intake were analyzed. Younger subjects had more animal fat proportion than older subjects did. HS and CS had the least fat proportion from fishes and shellfishes. Each mean cholesterol intake of PC, EC, HS, and CS was over 300mg/day, and HS load the highest level of 415mg/day. Cholesterol density of the diet of PC was the highest of 175mg/1000kcal and the level decreased when the age increased. The subjects consuming cholesterol over 100 mg/1000kca1 were about 70% of PC and HS. More than 20% of PC, EC, and HS consumed calcium under 75% of RDA with protein consumption over 125% of RDA. Dietary fiber intakes were 18g for HS, CS, 30-49 years, and 50 ∼ 69 years, 14g for EC and over 70 years, and 12g for PC. Ninety percent of PC and EC, 80% of HS and CS, and 70% of tole subjccts over 30years consumed dietary fiber under the level of 10g/1000kca1. The major food sources of dietary fiber were vegetables and grains. From these results, children and adolescents had ricks for chronic degenerative diseases on all the analyzed items. The results of this study would provide the basic information for development of dietary interventions to improve diet find health.

• Elastomers and Composites
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• 제8권1호
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• pp.33-51
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• 1973

We have studied the blending effects of Diene NF 35 R and Alfin 1530 at various blending ratios, 100/0, 70/30, 50/50, 30/70, 0/100, and of carbon black HAF-HS and ISAF-HS at various compounding ratios of 45 PHR, 55 PHR, 65 PHR, for tyre tread rubber. As the results, it was found that; 1. For tyre tread rubber, as the blending ratio, AR 1530/Diene NF 35R, indicated 70/30, the physical properties we examined were most excellent. 2. Excellent result was obtained in the case of carbon black compounding ratio of 55 PHR. The compounding of ISAF-HS made better result than that of HAF-HS for tensile strength, but the compounding of HAF-HS made better result than that of ISAF-HS for tearing strength and best result for abrasion quantity. 3. Heat buildup obtained from compounding carbon black HAF-HS indicated low temperature than that from compounding carbon black HAP-HS. As the compounding amount of carbon black increased, and as the blending amount of AR 1530 decreased, the heat buildup increased. 4. Carbon black was more efficient to AR 1630 than io Diene NF 35 R. 5. In the physical properties, mooney viscosity and mooney scorch time, as the compounding amount of carbon black increased, the values of mooney viscosity increased, but that of mooney scorch time had a max. point at the compoundiug amount of carbon black, 55 PHR.

• 한국생산제조학회지
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• 제7권1호
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• pp.95-103
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• 1998

This paper is concentraed on a study of operation for multiple objectives in a automated manufacturing system with multiple machining cells . Largest Sum Processing-time First(LSPF) was developed in order to minimize makespan, mean flowtime and maximize mean utilization and compare with Ho and Chang's algorithm(HC) and Hunsucker and Shah's algorithm(HS). The results show that LSPF provides better soutions than HC at 78.2% and than HS at 67.8% of total problems to frequency . LSPF reduces 5.8% of makespan by HC and 22.1% of the value by HS and curtails 15.8% , 7.5% of mean flowtime by receptive algorithms(HC, HS). And mean utilization is also higher about 5.5% than HC and HS.

• The Korean Journal of Pain
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• 제23권2호
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• pp.147-150
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• 2010

Background: Chronic low back pain can be a manifestation of lumbar degenerative disease, herniation of intervertebral discs, arthritis, or lumbar stenosis. When nerve roots are compromised, low back pain, with or without lower extremity involvement, may occur. Local inflammatory processes play an important role in patients with acute lumbosciatic pain. The purpose of this study was to assess the value of erythrocyte sedimentation rate (ESR) and high sensitivity C-reactive protein (hsCRP) measurements in patients with chronic low back pain or radiculopathy. Methods: ESR and hsCRP were measured in 273 blood samples from male and female subjects with low back pain and/or radiculopathy due to herniated lumbar disc, spinal stenosis, facet syndrome, and other diseases. The hsCRP and ESR were measured prior to lumbar epidural steroid injection. Results: The mean ESR was 18.8 mm/h and mean hsCRP was 1.1 mg/L. ESR had a correlation with age. Conclusions: A significant systemic inflammatory reaction did not appear to arise in patients with chronic low back pain.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2009년도 제40회 하계학술대회
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• pp.1289_1290
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• 2009

유해물질 제한지침(Restriction of Hazardous Substances Directive)은 유럽연합(EU)에서 시행되며 해로운 물질을 사용한 전자제품이나, 전기기기를 제한하는 지침이다. 일반적으로 RoHS(알오에이치에스)라고 적는다. 2003년 2월에 WEEE(Waste Electrical and Electronic Equipment)에 의해 제정 공포되고 2006년 7월 1일 발효되어 카드뮴, 납, 수은, 6가 크롬, 브롬계 난연제(PBBs, PBDEs)의 사용이 금지되었다. 중국에서도 이와 비슷한 제도 RoHS(일명 China RoHS)를 시행을 하고 있어 유럽에서 정한 6가지 품목과 규제 대상이 아니지만, 과학기기나 의학장비도 중국의 RoHS에는 규제 대상에 포함되어 있다.

• 대한간호학회지
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• 제25권2호
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• pp.193-209
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• 1995

The purpose of this study was to determine the effect of intermittent low - intensity, short duration exercise during hindlimb suspension on the mass, relative weight, myofibrillar protein content, cross-sectional area of Type I and Type II fibers and SDH activity in Type II(plantaris) muscle. To examine the effectiveness of intermittent low-intensity, short duration exercise on mass, myofibrillar protein content and fiber size, the hindlimbs of adult female Wistar rats were suspended(HS) and half of these rats walked on a treadmill for 45 min/day(9 min every 2h) at 5m /min and a 15$^{\circ}$grade (HS-EX). Plantaris wet weight was 19.67% significantly smaller(p<0.005) and relative plantaris weight was 6.25% smaller compared with those of control rats following seven days of hindlimb suspension. Plantaris wet weight and relative plantaris weight increased by 27.66%, 12.22% each through intermit-tent exercise during hindlimb suspension(p<0.005, p<0.05), moreover, plantaris wet weight and relative plantaris weight of the HS-EX rats were similar to those of control rats. Soleus wet weight and relative soleus weight decreased significantly by 31% and 22.0% in the HS rats(p<0.05). Soleus wet weight and relative soleus weight increased by 10.41%, 25.64% respectively through intermittent ex-ercise during hindlimb suspension, furthermore, soleus wet weight and relative weight of the HS-EX rats were closer to those of control rats. Myofibrillar protein content of plantaris and soleus decreased significantly by 51.49%, 59.65% each, following seven days of hindlimb suspension (p<0.005) Myofibrillar protein content of plantaris and soleus increased by 51.79%, 75.47% each with significance through intermittent exercise during hindlimb suspension(p<0.005). Myofibrillar protein content of plantaris and soleus in HS-EX rats was smaller than that of control rats. No change was observed in fiber type percentage following 1 week of hindlimb suspension or exercise during hindlimb suspension. The type I fiber cross-sectional area of both soleus and plantaris muscle was 18.72% and 41.07% lower in the HS than that of the controls (p<0.05, p<.001), that of both muscles was 6.60% and 29. 3％ greater in the HS-EX than that of the HS rats. HS plus intermittent low- intensity short duration exercise resulted in Type I fiber cross-sectional area closer to the controls. Type II fiber cross-sectional area of both plantaris and soleus muscle was 22.45% and 22.58% sl nailer in the HS than in the controls, that of both muschles in the HS-EX was 14.10%, 5.78% greater than HS. Intermittent exercise during hindlimb suspension resulted in Type I, II fiber cross-sectional area closer to the control value. There was no change in SDH activity following 1week of hindlimb suspension or exercise during hindlimb suspension in the plantaris muscle. The results suggest that intermittent low intensity short duration exercise can ameliorate Type II muscular atrophy Induced by hindlimb suspension.