• 한국수산과학회지
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• 제15권3호
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• pp.179-184
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• 1982

Lartobacillus bulgricus를 glucose를 제한기질로 하는 합성배지에서 연속배양한 결과를 요약하면 다음과 같다. 1. 본 실험 model은 Monod의 chemostat 이론을 적용시킬 수 있다. 2. 본 실험 model에서의 최대 cell production rate는 $0.178 g/1{\cdot}hr$로서 회석율 $0.414hr^{-1}$ 일 때이다. 3. 연속배양 결과 saturation constant($K_s$)는 7.69g/l, 최대비증식속도$(V_{max})$는 $0.62hr^{-1}$ 이었다. 4. wash out 현상은 $0.51hr^{-1}$에서 일어났으며cell yield coefficient는 0.016g/l 이었다.

• 한국산업보건학회지
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• 제9권1호
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• pp.14-22
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• 1999

This study was conducted to evaluate workplace protection factors(WPF) for two half-facepiece respirators (HR-1, HR-2) in welding workplace and to provide data on the workplace performance of negative-pressure, half-facepiece respirators against airborne particulate contaminants. The outside iron oxide(Fe2O3) concentration of welding fume for the respirator HR-1 ranged from 0.177 to $12.508mg/m^3$ with a geometric mean of $1.118mg/m^3$ and the HR-2 respirator showed a iron oxide range of 0.500 to $3.494mg/m^3$ with a geometric mean of $1.082mg/m^3$. But the inside oxide concentration of welding fume for the respirator HR-1 ranged from 0.002 to $0.364mg/m^3$ with a geometric mean of $0.019mg/m^3$ and the HR-2 respirator showed a iron oxide range of 0.012 to $0.639mg/m^3$ with a geometric mean of $0.041mg/m^3$. The iron oxide inside concentrations were significantly less than $5mg/m^3$(TLV) for both of respirators. The WPF were ranged from 3 to 3744 with a geometric mean of 60 for HR-1 and range from 2 to 129 a geometric mean of 26 for HR-2. And, in this study, the 5th percentile of the workplace protection factors for half-facepiece aspirators (HR-1, HR-2) were 11.2 and 7.1, respectively. The correlation relationship between the Quantative Fit Factors(QNFT) and the WPF for half-mask negative pressure respirators were 0.099 and 0.460.

• 대한방사선기술학회지:방사선기술과학
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• 제33권3호
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• pp.269-276
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• 2010

본 연구는 고용량 방사성옥소 치료환자의 선량률을 거리와 시간대별로 산출하여 방사성옥소 치료를 위한 입원기간의 최적화와 개선방안을 제한 하고자 하였다. 그 결과 100 mCi 고용량 옥소치료환자의 24시간 후에 배꼽에서 $1,035{\mu}Sv/hr$, 50 cm에서 $109{\mu}Sv/hr$, 100 cm에서 $33{\mu}Sv/hr$로 감쇠 되었다. 150 mCi 고용량 옥소치료환자의 24시간 후에 배꼽에서 $637{\mu}Sv/hr$, 50 cm에서 $100{\mu}Sv/hr$, 100 cm에서 $40{\mu}Sv/hr$로 감쇠 되었다. 180 mCi 고용량 옥소치료환자의 24시간 후에 배꼽에서 $1,251{\mu}Sv/hr$, 50 cm에서 $140{\mu}Sv/hr$, 100 cm에서 $56{\mu}Sv/hr$로 감쇠 되었다. 퇴원기준을 미국 원자력 규제위원회 고시에 $70.4{\mu}Sv/hr$이므로 본 연구 결과보다 빠른 퇴원이 가능하다. 치료 환자들의 계속적인 증가 추세로 볼 때 치료병실의 부족을 해소 할 수 있다.

• The Korean Journal of Physiology and Pharmacology
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• 제3권2호
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• pp.231-236
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• 1999

This study was performed to examine 1) Whether hypothermic cardiac arrest produces myocardial HSP72 expression; 2) And if, whether it serves to protect the heart against the subsequent hypothermic arrest. In the present study, neonatal rats were placed in an icebath to induce hypothermia. To determine whether hypothermic cardiac arrest produces myocardial HSP72, experimental animals were subjected to 10-min hypothermic insult before the extraction of the heart. The intervals between the insult and extraction were 1 (1 HR), 4 (4 HR), 8 (8 HR), 24 (24 HR) or 72 (72HR) hours. A minimal amount of HSP72 was detected in control, 1 HR and 72 HR groups. In contrast, 8 HR and 24 HR groups showed a significant level of HSP72 expressions. To assess the cardioprotective effect of HSP72 against hypothermic cardiac arrest, we compared the proportion of recovery from the arrest between control and preconditioned (PREC) animals. Control animals were subjected to 20-min hypothermic insult, while PREC group was preconditioned by 10-min hypothermic insult 8 hours before the 20-min test hypothermic insult. Resuscitation rate from cardiac arrest induced by the 20-min hypothermic insult in PREC group was significantly higher than that in controls. These results suggest that the cardioprotective effect of hypothermic preconditioning is associated with an increase in HSP72 expression.

• 약학회지
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• 제44권3호
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• pp.232-236
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• 2000

An intravenous pharmacokinetics for a new red cell substitute, PEG-hemoglobin SB1, was studied in SD rats. Total-hemoglobin and its metabolite methemoglobin in the plasma were determined using a spectrophotometer. The limit of quantitation was 0.01g/dL and the C.V for interday assay reproducibility was less than 6%. Upon intravenous administration of anticipated clinical dose, 10 ml(0.7 gHb)/kg, plasma concentration curve of total-hemoglobin was well described by one-compartment model. The $t_{\frac{1}{2}},{\;}CL_{t}$, Vd and $AUC^{0-48hr}$ were $8.23{\pm}0.96{\;}hr,{\;}0.06{\;}{\pm}{\;}0.01 {\;}dL/hr/kg,{\;}0.66{\pm}0.05{\;}dL/lg{\;}and{\;}13.6{\;}{\pm}{\;}1.01g{\cdot}hr/dL$, respectively, in male rats(n=5, $mean{\;}{\pm}{\;}SD$). Those parameters in female rats were $9.21{\;}\pm{\;}2.31{\;}hr,{\;}0.06{\;}{\pm}{\;}0.01{\;}dL/hr/kg,{\;}0.79{\pm}{\;}0.08{\;}dL/kg{\;}and{\;}13.0{\;}{\pm}{\;}2.36{\;}g{\cdot}hr/dL$, respectively. Similar kinetic profiles between males and females were also obtained from other parameters. Small amount of methemoglobin, an oxidative metabolite of SB1, was detected in the plasma of both sexes, where the $AUC^{0-48{\;}hr,m}$ and $t_{{\frac{1}{2}},m}$ were approximately $1.5{\;}g{\cdot}hr/dL$ and 20 hr, respectively. The present work provides a critical kinetic data for the effective clinical applications of PEG-hemoglobin SB1.

• 한국환경농학회지
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• 제26권3호
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• pp.197-203
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• 2007

본 연구의 목적은 비소로 오염된 수질을 영가철(ZVI)을 이용하여 복원하는 과정에서 pH, 온도, 초기 비소농도, 영가철량 등 다양한 환경요인별 영가철 기술의 효율성을 평가하는데 있다. 영가철에 의한 비소의 불용화 반응은 일차반응속도를 따랐으며 반응상수(k)는 ZVI의 처리농도가 증가할수록(1%: 0.158, 2%: 0.257, 3%: 0.342 $hr^{-1}$), 그리고 반응온도가 높을수록($15^{\circ}C$: 0.117, $25^{\circ}C$: 0.202, $35^{\circ}C$: 0.246 $hr^{-1}$)증가하였다. 반면 비소의 불용화 반응은 초기 As(V)의 농도가 낮을수록(1 mg $\Gamma^{-1}$: 0.284, 2 mg $\Gamma^{-1}$: 0.202, 3 mg $\Gamma^{-1}$: 0.153 $hr^{-1}$), 그리고 반응 pH가 낮을수록(pH 3: 0.393, pH 5: 0.213, pH 7: 0.097, pH 9: 0.067 $hr^{-1}$)증가하였다. 이상의 연구 결과를 통하여 비소로 오염된 물에 다양한 환경조건(pH, 처리량, 오염물질의 농도, 반응온도) 하에서 영가철을 적용하기 위한 최적 인자를 도출하였다.

• 한국식품영양과학회지
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• 제13권4호
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• pp.363-371
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• 1984

The content of total lipid, cholesterol and fatty acid in Korean styled various beef broths was examined before and during toiling to determine the influence of cooking time. The values obtained from the broths of brisket, ox-tail and small-intestine were determined by using G. C. The highest total lipid value among small-intestine, brisket and ok-tail was in the small-intestine. The content of total lipid in the boiled meat decreased during cooking with the exception of the brisket. Total lipid content found in the broth of brisket meat increased until 6hr and thell decreased at cooking time of 8hr and 10 hr. The highest total lipid contents in the broths of brisket, ox-tail and small-intestine were found when the cooking time were 6 hr, 10 hr and 4 hr, respectively. The main fatty acids found in the broths of brisket, ok-tail and small-intestine were $C_{14}$, $C_{16}$, $C_{18}$, $C_{18:1}$, and $C_{18:1}$. The content of $C_{18:1}$ and $C_{18:2}$ from the brisket meat increased until 4hr's cooking, especially $C_{18:2}$ increased significantly until 6 hr cooking. The highest ratios of unsaturated to saturated fatty acid (UNS/S) in the meat of brisket and ox-tail were found in the 2hr and 4hr cooking, respectively. The ratios of UNS/S in the broths of both of brisket and small-intestine, and ok-tail were highest in the 4 hr and 2 hr cooking, respectively. The content of total cholesterol (TC) was the highest in the small-intestine and the highest percentage of ester cholesterol was 5.4 in the brisket. The content of TC in small-intestine, brisket and ox-tail was decreased significantly after 2 hr cooking. Precentage of the ester in the brisket was the highest when the cooking time was 8 hr. Only trace amount of total cholesterol was found in the broth.

• 생명과학회지
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• 제17권2호통권82호
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• pp.174-178
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• 2007

malformin A1은 옥수수 뿌리에서 대조구와 비교하여 에틸렌 생성을 4시간에는 130%, 8시간에는 56%를 각각 촉진하였다. 에틸렌 생성량이 4시간을 최대로 하여 8시간까지 서서히 증가율이 감소하였다. 칼슘 이온 조절제인 A23187 (calcium ionophore)과 verapamil (calcium channel blocker)을 각각 단독으로 처리한 경우 모두 대조구와 비교하여 4시간에는 30%, 8시간에는 20% 정도로 에틸렌 생성을 촉진하였다. 칼슘 이온 조절제가 malformin A1에 의해 유도된 에틸렌 생성에 미치는 영향을 조사한 결과 4시간에는 malformin A1만 처리하였을 경우에 관찰되는 수준만큼 촉진되지 않았지만 칼슘 이온 조절제의 촉진 효과는 8시간까지 유지되었다. 이러한 결과는 malformin A1이 에틸렌 생성을 촉진하기 위하여 적절한 칼슘 농도가 필요할 가능성을 제시하고 있다. Malformin A1은 굴중성 굴곡 반응을 대조구와 비교하여 4시간과 8시간에 각각 58%와 42% 정도 억제하였다. A23187과 달리, verapamil은 굴중성 굴곡반응을 4시간과 8시간에 대조구와 비교하여 각각 54%와 23%를 억제하였다. 더 나아가 verapamil은 malformin A1에 의해 유도된 굴중성 굴곡 반응을 A23187보다 더 억제하였다. 이러한 결과는 칼슘이온 조절제가 malformin A1에 의해 유도된 옥수수 뿌리의 굴중성 반응에 영향을 주며, 칼슘 이온이 굴중성 반응에 중요한 역할을 한다는 증거를 제시한다.

• 한국수산과학회지
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• 제36권3호
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• pp.276-282
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• 2003

Using Megabalanus rosa a sessile filterfeeder, its scope for growth (SFG) by analysis of energy budget were examined and free amino acids composition of whole body tissue were analyzed under the exposure to different concentrations of Hg Cu and Cd. The $96\;hr-LC_{50}$ of the barnacle after 96 hr exposure to Hg, Cu, and Cd were 0.220, 0.269 and $1.380\;mgL^{-1}$, respectively. Hg and Cu showed stronger toxicity than Cd, while Hg and Cu had similar influence on the survival of the barnacle. SFG of the barnacles exposed to sublethal concentrations of mercury was 18.936 $Jgdrywt.^{-1}hr^{-1}$ in control group and as increase of mercury concentration the SFG remarkably reduced to 0.041 $Jgdrywt.^{-1}hr^{-1}\;at\;0.1\;mgL^{-1}$ concentration of Hg. In the case of Cu, the SFG was 29.841 $Jgdrywt.^{-1}hr^{-1}$ in control group and as increase of concentration, the SFG remarkably reduced to -8.304 $Jgdrywt.^{-1}hr^{-1}\;at\;0.1\;mgL^{-1}$ concentration. In Cd the SFG was 15.852 $Jgdrywt.^{-1}hr^{-1}$ in control group, and as increasing concentration, the SFG remarkably reduced to -19.490 $Jgdrywt.^{-1}hr^{-1}\;at\;0.1\;mgL^{-1}.$ Content of free amino acid (FAA) of whole body tissue of the barnacle was 45084 $mgkg^{-1}$ in control group, but it was reduced remarkably to 28,130, 37,500 and 37,106 $mgkg^{-1}$ at 0.1 $mgL^{-1}$ concentration of Hg and Cu, and 0.4 $mgL^{-1}$of Cd, respectively. Sum of threonine + serine was 1,334 $mgkg^{-1}$ if control but reduced remarkably to 1,223, 849 and 888 $mgkg^{-1}$ at 0.1 $mgL^{-1}$ of Hg and Cu, and 0.4 $mgL^{-1}$ of Cd, respectively.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1994년도 춘계학술대회 and 제3회 신약개발 연구발표회
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• pp.201-201
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• 1994

항암 및 면역조절작용을 가지고 있으며 그자체가 서방성 prodrug으로서 약효를 나타낼것으로 기대되는 ara-C와 etherphospholipid의 conjugate인 ara-CDP-DL-PCA.2Na의 실험동물(rat)에서의 체내동태를 HPLC 정량법으로 시행 하였다. Ara-CDP-DL-PCA.2Na의 정맥내 투여(20mg/300g)의 경우 혈장중 반감기는 분포상에 있어서는 평균 20min, 소실상 에서는 4.42hr이었다. 분포 용적은 0.1635Lㆍkg$^{-1}$이었고, 전신 clearance 는 25.63ml.$hr^{-1}$.kg$^{-1}$이었다. 한편 AUC는 2601$\mu\textrm{g}$.hr.ml$^{-1}$이었다. 복강내 주사(1g/kg)의 경우 소실상의 반감기는 3.28hr, AUC는 439.3$\mu\textrm{g}$.hr.ml$^{-1}$이었다. 최고 혈중농도 도달시간은 4.4hr이었으며 그때의 농도는 33.19$\mu\textrm{g}$.ml$^{-1}$이었다. 한편 흡수속도 정수는 0.2493$hr^{-1}$이었다. 정맥내 투여에 대한 복강내 투여의 생체 이용율은 1.1252%로 복강내 투여시 흡수율이 좋지 않음을 시사하고 있다. 이는 투여 시료인 micellar solution이 일종의 약물저장 형태로 작용하여 활성물질을 서서히 방출 하기 때문인 것으로 사료된다. 한편 예비적 대사연구결과 TLC에서 뇨나 담즙으로 배설되는 유의한 농도수준의 ara-CDP-DL-PCA.2Na가 없는 것으로 평가되었다. 이는 생체내에서 enzyme등에 의한 대사과정을 거치는 것으로 사료되는바 방사성 동위원소 표지화합물을 이용한 충분한 대사연구가 필요한 것으로 사료된다.