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Alterations of Blood Cholinesterase Activity and Urinary Para-nitrophenol Excretion After Exposure to Organophosphorus Insecticides (유기인제 농약 폭로로 인한 혈중 Cholinesterase 활성치와 요중 P-nitrophenol의 배설량의 변동)

  • Lee, Byung-Kook;Chung, Kyou-Chull
    • Journal of Preventive Medicine and Public Health
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    • v.7 no.1
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    • pp.115-121
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    • 1974
  • In order to evaluate the health hazard due to exposure to organophosphorus insecticides, we measured the blood cholinesterase activity ana urinary para-nitrophenol among 56 exposed subjects. They are orchard workers, rice plant workers and smithion factory workers. The clinical symptoms were also checked by physicians. We also measured the blood cholinesterase activity and urinary para-nitrophenol excretion of 20 urban people and 15 rural people who had never been exposed to organophosphorus insecticides in order to compare them according to age, sex and geographical differences. And these results were also compared with those of exposed groups. The results obtained were as follows. 1. The normal plasma cholinesterase activity and cell cholinestrase activity were $0.861{\pm}0.148\;{\Delta}pH/hr$ and $0.822{\pm}0.154\;{\Delta}pH/hr$. And normal para-nitrophenol in urine was $1.21{\pm}0.52mg/liter$. 2. No significant difference was existed in blood cholinesterase activities and urinary para-nitrophenol excre tion according to sex, age and geographical difference. 3. The plasma cholinesterase activity and cell cholinesterase activity of orchard workers, rice plant workers and smithion factory workers were $0.682{\pm}0.189\;{\Delta}pH/hr,\;0.775{\pm}0.160\;{\Delta}pH/hr,\;0.754{\pm}0.123\;{\Delta}pH/hr,\;and\;0.691{\pm}0.082\;{\Delta}pH/hr,\;0.756{\pm}0.117\;{\Delta}pH/hr,\;0.739{\pm}0.117\;{\Delta}pH/hr$. And significant decreses in blood cholinesterase activities were existed among orchard workers and smithion factory workers compared with control group. 4, The urinary para-nitrophenol excretions of orchard workers, rice plant workers and smithion factory workers were $1.33{\pm}0.66mg/liter,\;1.19{\pm}0.88mg/liter\;and\;1.37{\pm}0.67mg/liter$ and there were no significant difference between exposed groups and control group. 5. The clinical symptoms complained during and after organophosphorus insecticides exposure were frequently ranked by headache (67.7%) and vertigo (64.5%) and muscular ataxia and weakness (51.6%).

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Influence of Midazolam and Glycopyrrolate on Intra-operative Body Temperature in Abdominal Surgical Patients

  • Kim, Eun-Ju;Yoon, Hae-Sang
    • Journal of Korean Biological Nursing Science
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    • v.14 no.1
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    • pp.25-32
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    • 2012
  • Purpose: influence of benzodiazepine (midazolam)or cholinergic inhibitor (atropine or glycopyrrlate) on intra-operative body temperature remains unclear and controversial. This study compares intra-operative body temperature in 50 abdominal surgical patients under general anesthesia between the administration of midazolam and glycopyrrolate in combination, or glycopyrrolate alone. Methods: Patients who underwent abdominal surgery were recruited from September 2008 through October 2009 at Gachon University Gil hospital in incheon. Core body temperature was measured in the right ear using a tympanic membrane thermometer at induction of general anesthesia and at 1 hr, 2 hr, and 3 hr after induction. Results: There were no differences in core body temperature at any measurement point between either patient group (F=1.08, $p$=.377). Core body temperature decreased throughout the 3 hr after induction in both groups (F=9.22, $p$ <.001). Specially, core temperatures at induction of general anesthesia (p<.001), 1 hr (p<.001), 2 hr ($p$ <.001), and 3 hr ($p$ <.001) after induction were lower than before administration of midazolam and glycopyrrolate, or glycopyrrolate alone. Conclusion: We conclude that a cholinergic inhibitor (glycopyrrolate, 0.1 mg) therefore seems not to affect intra-operative body temperature of patients given a benzodiazepine (midazolam, 0.04 mg $kg^{-1}$), and not to increase body temperature in patients not given a benzodiazepine during the 3 hr after the induction of general anesthesia. Intra-operative warming therefore is needed to prevent hypothermia in surgical patients who receive pre-operative administration of midazolam and/or glycopyrrolate.

Effect of Anti-juvenile Hormone Analogue (AJH) on the Larval Ecdysis of the Silkworm, Bombyx mori L. (항유약호르몬 활성물질이 누에탈피에 미치는 영향)

  • 홍성진;이화준
    • Journal of Sericultural and Entomological Science
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    • v.39 no.2
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    • pp.161-168
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    • 1997
  • To clarify the effect of anti-juvenile hormone analogue (AJH) on the larval ecdysis by feeding at early stage of the 4th instar, the total amount of protein and activity of chitinolytic enzymes in the integument of Bombyx mori were analyzed, PAGE pattern of the protein was observed and the morphological changes of integument during molting period were also observed and the morphological changes of integument during molting period were also observed by means of TEM. The total amount of protein was greatly increased in premolting, then reached maximum level just before ecdysis, and rapidly decreased after the larval ecdysis in the control, while in the AJH treatment, increased 12 hr later than the control and its maximum was only 82.6% of the control. Two specific proteins, which were presumed as the protein originated from endocuticle, also appeared 12 hr later than the control and were maintained to 132 hr after AJH treatment from the aspects of the Native- and SDS-PAGE patterns, although those of the control disappeared instantly after ecdysis. Chitinase and $\beta$-N-acetylglucosaminidase activities were also suppressed and delayed by AJH treatment. Furthermore, it was observed that the apolysis took place 12 hr later than the control but new epicuticle was not formed at least until 132 hr after AJH treatment. From these results, it is suggested that the larval molting process of silkworm develops 12 hr later than the control but new epicuticle was not formed at least until 132 hr after AJH treatment. From these results, it is suggested that the larval molting process of silkworm develops 12 hr later than the control by AJH treatment but no further processing takes place just after apolysis.

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Biofiltration of soil Vapor Extraction Off-gas from Gasoline Contaminated Soil Using a Compost (퇴비를 이용한 가솔린 오염토양증기추출 배가스의 바이오필터 처리)

  • 남궁완;박준석
    • Journal of Korea Soil Environment Society
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    • v.4 no.3
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    • pp.25-33
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    • 1999
  • The recent emergence of biofiltration as a cost effective waste-gas control technology has stimulated in European countries and the USA. Biofiltration of soil vapor extraction off-gas from gasoline contaminated site was simulated in lab-scale in this study. A filling material used was a compost. This study was conducted to evaluate biofiltration characteristics of the compost material for gasoline off-gas. TPH elimination capacity at the gas loading rate of about 50g/$\textrm{m}^3$((filling material)/hr was circa 40g/$\textrm{m}^3$((filling material)/hr. Removal rate of xylene was the highest among BTEX. while it was the lowest in case of bezene. The maximum elimination capacity of the compost was about 1.5g/$\textrm{m}^3$((filling material)/hr for benzene. More than 95% of trimethylbenzene and naphtalene were removed below the loading rate of 0.7g/㎥(filling material)/hr. About 80% of total TPH and BTEX were removed by biodegradation.

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Monitoring on Alcohol Fermentation Characteristics of Strawberry (딸기의 알콜 발효 특성 모니터링)

  • Lee, Jin-Man;Kim, Suk-Kyung;Lee, Gee-Dong
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.32 no.5
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    • pp.679-683
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    • 2003
  • Strawberries were fermented for their effective value added product. In alcohol fermentation of strawberries, alcohol content was maximum value (9.22% ) under the condition of 16.32$^{\circ}$Brix of initial sugar concentration, 53.03 hr of fermentation time and 28.8$^{\circ}C$ of fermentation temperature. Acetic acid content revealed minimum value (0.49%) under the condition of 13.18$^{\circ}$Brix of initial sugar concentration, 50.99 hr of fermentation time and 24.96$^{\circ}C$ of fermentation temperature. Residual sugar content revealed minimum value (3.97$^{\circ}$Brix) under the condition of 15.00$^{\circ}$Brix, 52.00 hr and 26.0$0^{\circ}C$. The optimum conditions for each alcohol fermentation were 14$^{\circ}$Brix, 50 hr and 28$^{\circ}C$.

Development of the Sub-soil Storage System for Utilization Urban Instream Flow of Rainfall Runoff (우수유출수의 도시하천 유지유량 활용을 위한 지하저류시스템 개발)

  • Choi, Gye-Woon;Choi, Jong-Young;Kim, Suk-Dong
    • Journal of Korea Water Resources Association
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    • v.37 no.2
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    • pp.163-172
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    • 2004
  • In this paper, the sub-soil storage system for utilizing urban instream flow of rainfall runoff was developed and examined through experiments. The artificial rainfall facility and sub-soil storage were installed in the experimental area. The effect of the water qualify improvement and the storage effect were analyzed through the several experiments. Through the experiments of rainfall intensity variation, which are the rainfall intensity of 20mm/hr, 30mm/hr, 40mm/hr, 50mm/hr was indicated SS concentration can be reduction until 68%. Also, the ration of the storage volume is varied from 42.8%∼79.9% based on the rainfall intensity. The reduction rate of the BOD, CO $D_{Mn}$, SS, T-N, T-P was 30%, 42%, 68%, 39%, 26%. As the result, water quality of runoff and efficient of runoff reduction by the system are much improved. The rainfall runoff with the installation of sub-soil storage could be used for instream flow.

Enhanced Expression of Angiotensinogen mRNA in Rat Central and Peripheral Tissues Following Hemorrhage

  • Do, Eun-Ju;Yang, Eun-Kyoung;Kim, Kyung-Soon;Kim, Suk-Hee;Park, Yoon-Yub;Ahn, Dong-Kuk;Park, Jae-Sik;Lee, Won-Jung
    • The Korean Journal of Physiology
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    • v.29 no.2
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    • pp.259-267
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    • 1995
  • The renin-angiotensin system plays an important role in the regulation of blood pressure and in body fluid homeostasis. There is increasing evidence for generation of endogenous angiotensin II in many organs and for its role in paracrine functions. Studies were designed to investigate whether hemorrhage produces rapid changes in the gene expression of angiotensinogen in peripheral and brain tissues. Wistar rats received saline drinking water for 7 days, were bled at a rate of $3\;ml\;kg^{-1}\;min^{-1}$ for 7 min, and then decapitated 0, 2, 4, 8, or 24 hr after hemorrhage. Hemorrhage produced a produced hypotension with tachycardia at $2{\pm}8\;hr$, but blood pressure and heart rate had not fully recovered to the basal level at 24 hr. Plasma renin concentration was significantly increased at 2, 4, and 8 hr (maximum sixfold increase at 4 hr) and had returned to the basal level at 24 hr. Renal renin content was significantly increased only at 4 hr after hemorrhage. Angiotensinogen mRNA in both the kidney and liver were stimulated at 2 to 8 hrs, but recovered to the basal level at 24 hr. On the other hand, angiotensinogen mRNA levels il the hypothalamus and brainstem were continuously increased from 2 to 24 hrs. The present study demonstrates the presence of angiotensinogen mRNA in both hepatic and extrahepatic tissues, and more importantly, their up-regulation after hemorrhage. These results suggest that the angiotensinogen-generating systems in the liver, kideny and brain are, at least in part, under independent control and play a local physiological role.

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Development of Physiological and Biochemical Bioindicators of Barnacle, Megabalanus rosa for Marine Pollution Assessment (해양오염 평가를 위한 빨강따개비(Megabalanus rosa)의 생리생화학적 오염지표의 개발)

  • LEE Jang-Won;CHIN Pyung
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.36 no.3
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    • pp.276-282
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    • 2003
  • Using Megabalanus rosa a sessile filterfeeder, its scope for growth (SFG) by analysis of energy budget were examined and free amino acids composition of whole body tissue were analyzed under the exposure to different concentrations of Hg Cu and Cd. The $96\;hr-LC_{50}$ of the barnacle after 96 hr exposure to Hg, Cu, and Cd were 0.220, 0.269 and $1.380\;mgL^{-1}$, respectively. Hg and Cu showed stronger toxicity than Cd, while Hg and Cu had similar influence on the survival of the barnacle. SFG of the barnacles exposed to sublethal concentrations of mercury was 18.936 $Jgdrywt.^{-1}hr^{-1}$ in control group and as increase of mercury concentration the SFG remarkably reduced to 0.041 $Jgdrywt.^{-1}hr^{-1}\;at\;0.1\;mgL^{-1}$ concentration of Hg. In the case of Cu, the SFG was 29.841 $Jgdrywt.^{-1}hr^{-1}$ in control group and as increase of concentration, the SFG remarkably reduced to -8.304 $Jgdrywt.^{-1}hr^{-1}\;at\;0.1\;mgL^{-1}$ concentration. In Cd the SFG was 15.852 $Jgdrywt.^{-1}hr^{-1}$ in control group, and as increasing concentration, the SFG remarkably reduced to -19.490 $Jgdrywt.^{-1}hr^{-1}\;at\;0.1\;mgL^{-1}.$ Content of free amino acid (FAA) of whole body tissue of the barnacle was 45084 $mgkg^{-1}$ in control group, but it was reduced remarkably to 28,130, 37,500 and 37,106 $mgkg^{-1}$ at 0.1 $mgL^{-1}$ concentration of Hg and Cu, and 0.4 $mgL^{-1}$of Cd, respectively. Sum of threonine + serine was 1,334 $mgkg^{-1}$ if control but reduced remarkably to 1,223, 849 and 888 $mgkg^{-1}$ at 0.1 $mgL^{-1}$ of Hg and Cu, and 0.4 $mgL^{-1}$ of Cd, respectively.

AN EXPERIMENTAL STUDY OF BOND STRENGTH OF GLASS IONOMER LUTING CEMENT UNDER THE FUNCTIONAL LOADS ON THE CROWN (기능 하중 하에서 접착용 Glass Ionomer 시멘트의 결합강도에 대한 비교연구)

  • Jun Joong-Hyun;Kang Woo-Jin;Lee Keun-Woo
    • The Journal of Korean Academy of Prosthodontics
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    • v.30 no.2
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    • pp.225-232
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    • 1992
  • The purpose of this study was to compare the bond strength of the glass ionomer luting cements after the functional loads were applied to the crowns respectively 10 min, 30 min, 1 hr, 24 hrs later after mixing of cements. The conventional (GC Fuji I; GC Int. Co., Japan), water hardening type (Ketac Cem; ESPE, W. Germany) glass ionomer luting cement and C-dent ZPC (Confi-Dental Products Co., U.S.A.) as a control were used. The results of this study were obtained as follows: 1. In the bond strength of GC Fuji I, 24 hr-group was highest and there were no statistically significant differences among 10 min-, 30 min- and 1 hr- groups. 2. l3 the bond strength of Ketac Cem and C-dent ZPC, there was no statistically significant differences among 10 min-, 30 min-, 1 hr- and 24 hr-groups respectively. 3. Comparing the bond strength among cements in 10 min-, 30 min-groups respectively, GC Fuji I was lowest and there was no statistically significant difference between Ketac Cem and C-dent ZPC. 4. The bond strengths between GC Fuji I and Ketac Cem were not significantly different in 1 hr-, 24 hr-group. 5. The bond strengths among 3 types of cements were not significantly different in 24 hr-groups.

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Influence of Pre-operative Fasting Time on Blood Glucose in Older Patients (수술 전 금식이 노인환자의 혈당에 미치는 영향)

  • Hong, Mi-Suk;Yoon, Hae-Sang
    • Journal of Korean Academy of Nursing
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    • v.41 no.2
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    • pp.157-164
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    • 2011
  • Purpose: This study was performed to identify changes in blood glucose at preoperative fasting time in surgical patients over 60 yr. Methods: Data collection was performed from July, 2008 through July, 2009. Participants consisted of 80 nondiabetic surgical patients. Blood glucose was checked from 3 to 5 times. The 5 times were 2-hr fasting on the pre-operative day (T1, n=80), 8 hr (T2, n=80), 10 hr (T3, n=17), 12 hr (T4, n=34) and 14 hr fasting on the day of the operation (T5, n=29). Results: Of the patients, 27.5% had a blood glucose level of less than 79 mg/dL at T2; 17.6% at T3; 32.4% at T4; and 17.2% at T5. Mean blood glucose levels were 93.8 mg/dL at T1; 88.4 mg/dL at T2; 91.7 mg/dL at T3; 87.4 mg/dL at T4: and 94.1 mg/dL at T5. Blood glucose was the lowest at T2 (p<.001). Conclusion: As 17.6-32.4% of the patients showed the blood glucose level of less than 79 mg/dL at 8-14 hr pre-operative fasting, the authors recommend that surgical patients p>60 yr-of-age be observed for hypoglycemia during pre-operative fasting of more than 10 hr and that surgical patients >60 yr-of-age with risks for hypoglycemia be scheduled for operation within 10 hr preoperative fasting.