In this study, changes of nutrients such as vitamins, minerals, and antioxidants were determined by using a multi-functional grinder. Contents of vitamin C and vitamin $B_1$ were measured by HPLC, and antioxidant activity was estimated toward free radical scavenging effect in using DPPH. Contents of minerals(Zn, Mn, Fe, Mg, K, and Ca) were computed by ICP-Mass. Results identified that the contents of vitamin C was $44.06{\pm}10.86mg/kg$ in mandarine, $132.1{\pm}22.80mg/kg$ in orange, and $12.79{\pm}2.01mg/kg$ in pineapple by using the hand blender for 3 minutes, and the loss rate of vitamin C contents were 12%, 7%, and 12% in comparison with the control group. In addition, the contents of vitamin C were $41.89{\pm}5.55mg/kg$ in mandarine, $131.51{\pm}12.67mg/kg$ in orange and $16.76{\pm}1.47mg/kg$ in pineapple when using of the grinder for 3 minutes, and the loss rate of vitamin C contents were 16%, 8%, and 17%. The results of vitamin $B_1$ showed a tendency to decrease in the same manner as the content of vitamin C even if there was no significant difference. Furthermore, the result of antioxidant activity revealed that free radical scavenging effect of sample was 60% decreased when using a hand blender and 10% decreased when using a grinder. Thus decrease rate of antioxidant activity when using the hand blender was higher than grinder. Lastly, current study could find any significant differences among the 16 food samples for cooking when employing the multi-functional grinder(p<0.05).
We investigated the content of sugar and sodium in 4 types of chicken (fried, seasoned, soy sauce-flavored, cheese powder-flavored). A total of 123 samples were collected from franchise stores and markets in Seoul. The sugar content of chicken samples was analyzed by HPLC-ELSD (High Performance Liquid Chromatography-Evaporative Light Scattering Detector). The average sugar content of seasoned chicken was highest at 8.7±2.3 g/100 g while that of fried chicken was lowest at 0.6±0.3 g/100 g. The average content of sugar sorted by place of sale showed a significant difference (P<0.05). The sodium content in chicken samples was analysed using the ICP-OES (Inductively Coupled Plasma-Optical Emission Spectrometry). The sodium content of seasoned chicken with cheese powder was the highest at 627.0±109.2 mg/100 g and that of fried chicken was the lowest at 448.0±65.3 mg/100 g. The sugar content of seasoned chicken sold in both franchise stores and markets was found to exceed the 50 g daily level as recommended by the WHO. In addition, the average sodium content in seasoned chicken (franchise stores and markets) and cheese-powdered chicken was more than twice as high as the daily 2,000 mg recommended by the WHO.
This study was done to analyze the contents of minerals and vitamins to compare the measured values of minerals, vitamins with labeled values of them in food labeling and to investigate the ratio of measured values to labeled values in 437 specimen with minerals and vitamins - fortified commercial beverages and liquid teas. Content of calcium and sodium in samples after microwave digestion was analyzed with an ICP-OES (Inductively Coupled Plasma Optical Emission Spectrometer) and vitamins were determined using by HPLC (High Performance Liquid Chromatography). The measured values of calcium were ranged 80.3~142.6% of the labeled values in 21 samples composed calcium - fortified commercial beverages and liquid teas. In case of sodium, measured values were investigated 33.9~48.5% of the labeled values in 21 sports beverages. The measured values of vitamin C, vitamin $B_2$ and niacin were ranged 99.7~2003.6, 81.1~336.7, 90.7~393.2% of the labeled values in vitamins - fortified commercial beverages and liquid teas, 57, 12, 11 samples. To support achievement of the accurate nutrition label, there must be program and initiatives for better understanding and guidances on food labelling and nutrition for food manufacture.
Gu, Ja Hea;Han, Seung-Kyu;Chang, Hyun;Lee, Byoung Il;Kim, Woo Kyung
Archives of Plastic Surgery
/
v.34
no.5
/
pp.543-550
/
2007
Purpose: Diabetic foot ulcer is a representative disease of chronic would with multiple defects of wound healing factors. Many nutrition factors have been known to be essential for wound healing, but objective data are lacking. The purpose of this study is to determine those factors essential for wound healing, and to find out which of those factors are lacking in diabetic foot ulcer patients through this pilot study. Methods: We studied 100 patients who visited our clinic from March 2005 to February 2006 for treatment of diabetic foot ulcers with a duration of more than 6 weeks. We checked serum levels of protein, albumin, vitamin A, C, E, iron, magnesium, zinc, copper and hemoglobin by drawing 23cc blood after 8 hours of fasting. Protein, albumin, iron, magnesium levels were measured by colorimetry; hemoglobin levels were measured by auto analysis. Vitamin levels were measured by high performance liquid chromatography (HPLC), copper and zinc levels were measured by Inductively coupled plasma (ICP). They were compared with normal values. The patients were divided by transcutaneous oxygen pressure levels, age and sex to study the effects of these parameters. Results: 76% and 61% of patients had within-normal range serum protein and albumin levels, respectively. Among vitamins, only the level of vitamin C was low in 55% of the patients. Levels of vitamin A, E were normal or high in 93% and 100% of patients. As for trace elements, levels of iron and zinc were low in 63% and 60% of patients, but levels of magnesium and copper were usually normal or high. Levels of vitamin C, iron and zinc were lower in the low-transcutaneous oxygen pressure group. There were no definite differences according to age and sex. Conclusion: Serum levels of Hb, vitamin C, iron, zinc were low in most diabetic foot ulcer patients. The deficit was very severe in the low-transcutaneous oxygen pressure group.
The aim of this study was to describe the effects of genetic factors on the chemical composition or metabolites of grapes harvested within the same region. Grapes were separated into pulp, skin, and seed, and physicochemical characteristics were compared among seven grape varieties. The sugar concentrations of the grape musts ranged from $15.17-20.93^{\circ}Bx$ with Seibel variety being highest at $20.93^{\circ}Bx$. pH ranges of grape musts were 3.46-4.02 and total acidity was highest with 1.05 in Steuben variety. Tartaric acid content was highest with 146.68, 500.10 mg/L in pulp and skin extract of Merlot variety. Malic acid content was highest in pulp extract of Seibel variety (1127.14 mg/L) and skin extract of Chardonnay variety (1720.06 mg/L). K content was highest with 379.13 and 828.01 mg/L in pulp and skin extract of Chardonnay variety. Ca content was highest in pulp extract of Kyoho variety (6.98 mg/L) and skin extract of Campbell Early variety (12.26 mg/L).
Seo, Jeong-Wook;Kim, Byoung-Gwon;Kim, Yu-mi;Lee, Se-Young;Kim, Na-Young;Lim, Hyoun-Ju;Gu, Dongchul;Hong, Young-Seoub
Journal of Environmental Health Sciences
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v.45
no.6
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pp.675-687
/
2019
Objective: Methyl bromide (MB) fumigant has been shown to be fatal to human when was exposed. However, it were still used the significant amount in quarantine disinfection sites. The purposes of this study were to assess the MB exposure status and characteristics by fumigation-related workers and to provide supporting data for management plan. Methods: For this study, the three groups related to fumigation work were composed. A total of 107 workers were directly exposed, 20 field inspectors were indirect, and 20 general quarantines were not exposed. The urinary bromide ion concentrations in each group were analyzed by using HPLC/ICP-MS, and the working characteristics were identified using the structured questionnaire. Results: The urinary bromide ion concentration in the exposed group of fumigation workers was higher than the indirect and non-exposed groups. In the work characteristics of workers, there was a significant tendency to increase urinary bromide ion concentrations with higher fumigation work years (≤4 years: 2.84 (1.13-7.11) mg/g cr, >4-15 years: 5.36 (4.37-6.57) mg/g cr, >15-37 years: 6.69 (5.27-8.49) mg/g cr, p=0.034). In the comparison of the average number of working days per month, the more working days, the higher the urinary bromide ion concentration was statistically significant (≤12.5 days: 2.59 (1.19-5.65) mg/g cr, >12.5-19.25 days: 5.46 (4.62-6.44) mg/g cr, >19.25-27.25 days: 7.93 (5.93-10.59) mg/g cr, p=0.002). Conclusion: This study was the first nationwide survey including biological monitoring in workers exposed to methyl bromide. The results of this study were expected to be used as a reference for workers' health rights in relation to fumigation, prevention of addiction accidents, and safe management plan.
Lee, Young Ju;Kim, Ae Kyung;Kim, Ouk Hee;Lee, Chun Young;Lee, Hyun Kyung;Jung, Sun Ok;Lee, Sae Ram;Kim, Hee Sun;Kim, Il Young;Yu, In Sil;Jung, Kweon
Korean Journal of Medicinal Crop Science
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v.24
no.2
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pp.121-128
/
2016
Background: Turmeric (Curcuma longa L.) that is used as a food material has antioxidant, anticancer and anti-inflammatory properties. Recently the demand for functional foods and drugs has increased. The present study was carried out to determined of contents of residual sulfur dioxide, heavy metals, ash, acid insoluble ash and curcuminoids in turmeric from the Seoul Yak-ryeong market. Methods and Results: A total of 31 samples were obtained. Residual sulfur dioxide was not detected in any samples. Heavy metals (arsenic, cadmium, lead and mercury) were analyzed by ICP-MS (Inductively Coupled Plasma Mass Spectrometer) and a mercury analyzer and were detected in the ranges of 0.00 - 0.28, 0.00 - 0.07, 0.00 - 0.29 and 0.002 - 0.027mg/g respectively. No significant difference were observed between the average heavy metal contents of domestic and imported tumeric. However, average content of ash in domestic samples (7.8%) were significantly higher than that in imported samples (6.1%), whereas that of curcuminoids was significantly higher in imported samples (47.6mg/g) than in domestic samples (11.2mg/g). The average content of acid insoluble ash was not significantly different between two sample types (0.9% in each). Conclusions: There are no specific standards for turmeric used as food materials. Therefore, this study can be provided as basic data for the establishment of quality standards for turmeric.
Kim, Da-Young;Kim, Ji-Young;Kim, Kye-Hoon;Kim, Kwon-Rae;Kim, Hyuck-Soo;Kim, Jeong-Gyu;Kim, Won-Il
Journal of Applied Biological Chemistry
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v.61
no.4
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pp.391-395
/
2018
There is an increasing concern over arsenic (As) contamination of paddy soil and rice with regard to food safety. This study was conducted to investigate total and inorganic As concentration in one hundred husked and polished rice samples collected at the non-contaminated paddy soil in Korea. Arsenic species in rice samples were extracted using 1% nitric acid ($HNO_3$) with a microwave oven and were measured using high performance liquid chromatography coupled with inductively coupled plasma-mass spectrometry. Mean concentrations of total As in husked rice and polished rice were 0.18 and $0.11mg\;kg^{-1}$, respectively. Also, average inorganic As concentrations in husked rice and polished rice were 0.11 and $0.07mg\;kg^{-1}$, respectively. These levels are lower than the standard guideline value 0.35 and $0.2mg\;kg^{-1}$ for inorganic As in husked and polished rice recommended by Codex Committee on Contaminants in Foods and Ministry of Food and Drug Safety, respectively. The mean of the inorganic As ratio for the total amount of As was 0.65 and 0.67 for husked rice and polished rice, respectively, and the range was from 0.08 to 1.0. For health risk assessment, the average value of cancer risk probability was $9.24{\times}10^{-5}$ and ranged from $2.30{\times}10^{-5}$ to $1.90{\times}10^{-5}$. Therefore, human exposure to As through dietary intake of surveyed rice samples might considered to be a low health risk.
Background: In South Korea, areas around abandoned metal mines are designated as regions with high arsenic (As) contamination. However, studies assessing urinary As exposure, As metabolism, and relevant genetic polymorphisms in residents of these metal mine areas are lacking. Objectives: To identify factors associated with As exposure and evaluate the effects of MTHFR, As3MT, and GSTO1 genetic polymorphisms on As metabolism in residents of abandoned metal mine areas by measuring urinary As species. Methods: Urinary As species (arsenite [As3+], arsenate [As5+], monomethyl arsonic acid, and dimethylarsinic acid) were isolated using high-performance liquid chromatography in combination with inductively coupled plasma mass spectrometry (HPLC-ICP-MS). Four genetic polymorphisms (MTHFR A222V, MTHFR E429A, GSTO1 A140D, As3MT M287T) were analyzed in 144 residents of four areas around abandoned metal mines. Results: The study sample was comprised of 34.7% men and 65.3% women, with a mean age of 70.7±10.9 years. The urinary inorganic As concentration was higher among those consuming more than half locally produced rice (0.31 ㎍/L) than those consuming less than half such rice (0.18 ㎍/L). The urinary dimethylarsinic acid concentration was higher in the group that had consumed seafood in the past day (31.68 ㎍/L) than in those who had not (22.37 ㎍/L). Furthermore, individuals heterozygous in the MTHFR A222V and GSTO1 A140D polymorphism had higher urinary arsenic species concentrations than did individuals with a wild type or homozygous for the variant allele. Conclusions: Consumption of locally produced rice was associated with inorganic As exposure, whereas seafood consumption was associated with organic As exposure among residents of abandoned metal mine areas. There was no clear association between MTHFR A222V and GSTO1 A140D polymorphisms and As metabolism.
An, Jae-Min;Chang, Soon-Young;Hwang, Hyang-Ran;Park, Dae-Han;Lee, Bom-Nae;Kim, Saet-Byeol;Lee, Gwang-Hee
Korean Journal of Environmental Agriculture
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v.39
no.1
/
pp.10-19
/
2020
BACKGROUND: This study was to carry out risk assessment of contamination of cadmium (Cd), lead (Pb), and inorganic arsenic (I-As) in agricultural products of 25 crops from the abandoned mine areas. The 36 typical investigation sites located in Gyeongbuk provincial area were selected by considering the heavy metal levels, that had been known that the amount of the heavy metals exceeded the contamination level based on the previous survey. METHODS AND RESULTS: Cadmium, lead, and total arsenic (T-As) concentrations were determined using microwave device and ICP-MS. Inorganic arsenic was determined by HPLC-ICP-MS. The limits of quantification for heavy metals were 0.59 ㎍/kg for Cd, 0.42 ㎍/kg for Pb, 0.55 ㎍/kg for T-As, and sum of As (III) (1.74 ㎍/kg) and As (V) (2.25 ㎍/kg) for I-As, respectively. The contents of Cd, Pb, and I-As (only rice) were N.D.-0.958 mg/kg, N.D.-0.227 mg/kg, and 0.082 mg/kg, respectively, in the agricultural products. For risk assessment, dietary exposures of heavy metals through usual intake were 5.20×10-4-7.15×100 ㎍/day for Cd, 7.00×10-5-7.75×10-1 ㎍/day for Pb, and 1.17×101 ㎍/day for I-As, taking 0.01-14.37%, 0.01-2.05%, and 15.16% as risk indices, respectively. CONCLUSION: It requires to consider the critical levels of heavy metals in agricultural products due to unexpectedly high levels in a few places, while concentrations of heavy metals in the samples were relatively low in most areas.
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