• BMB Reports
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• 제30권1호
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• pp.26-32
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• 1997

Of all HLA class I molecules, HLA-C gene products are most poorly understood because they express at a low level on the cell surface compared to HLA-A and -B. In order to identify serologically detectable and undetectable HLA-C antigens, we have established a DNA-based tissue typing method for the HLA-C locus by PCR-SSP (polymerase chain reaction-sequence specific primers). Genomic DNA prepared from Iymphoblastoid 21 B-cell lines and 120 Korean individuals by proteinase K digestion and pheno/chloroform extractions have been typed by PCR-SSP (23 primer mixes were used). The PCR-SSP results of control cell lines were discrepant from serology in 1 case among 21 cases: Cw6 which was negative by serology but positive by PCR-SSP (cell line: MANIKA). Twenty four HLA-Cw "blank" antigens among fifty Korean individuals were completely determined by PCR-SSP DNA typing. HLA-Cw*0101 (15.3%), Cw*1401 (12.3%) and Cw*0701 (11.7%) alleles were frequently found in 120 Korean individual samples. In conclusion. the high level of discrimination for HLA-C alleles may prove useful and informative in the study of transplant survival, and identify the importance of allelic differences, not readily detectable by serology, on host and donor compatibility.

• Asian Pacific Journal of Cancer Prevention
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• 제14권7호
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• pp.4427-4433
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• 2013

Cervical cancer is the second most common cancer in women. HLA class I and II alleles polymorphisms have been shown to be associated with cervical cancer risk, but results have varied among different populations. In this study, the HLA-A, -B, and -DRB1 alleles among 100 southern Chinese women with cervical squamous cell carcinoma (SCC) were compared to 254 controls. Our results showed that $B^*51$:01:02 allele frequency was significantly higher in patients with SCC than in healthy controls ($P=3.17{\times}10^{-5}$, $P_c$=0.005, OR=26.7). Statistical analysis also revealed a significantly decreased frequency of $B^*51$:01:02 ($P=7.01{\times}10^{-4}$, $P_c$=0.03, OR=0.12) in patients with SCC when compared with healthy controls. These results indicate that HLA-$B^*51$:01:02 may confer susceptibility to SCC and HLA-$B^*51$:01:02 may contribute to resistance to the development of SCC in Chinese women. None of the HLA-A-B or HLA-A-B-DRB1 haplotypes were significantly different in cases and controls after multiple testing corrections, indicating the individual allele associations to be independent of the identified haplotypes. These results support the hypothesis that some HLA-B alleles could be involved with susceptibility for developing SCC.

• 정보처리학회논문지D
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• 제9D권6호
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• pp.1137-1144
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• 2002

패더레이션의 개선 가능성은 구성된 멤버 패더레이트들에게 유연성과 적응성을 요구하게 된다. 더욱이 비 연동화 모델을 HLA(High Level Architecture) 패더레이트로 개발하고 이를 가변적인 특성 패더레이션에 연동되도록 하기 위해서는 더 많은 시간과 노력이 요구된다. 본 연구에서는 이러한 문제를 해결하는 방법으로 ROM(RTI Object Model) 프레임워크를 설계하고 구현하는 방법을 제시하였다. ROM은 RTI(Run-Time Infrastructure) 프로그래밍과 패더레이트 시뮬레이션 프로그래밍을 완벽하게 분리시킴으로써 가변성 있는 FOM을 지원할 수 있는 HLA 패더레이트 개발을 비용과 생산성 측면에서 획기적인 효율을 제공하게 되었다. 즉 ROM은 RTI와 패더레이트 사이에 RTI 서비스를 관리하는 관리 계층과 실제로 객체 및 강호작용을 갱신 또는 반영하는 Foundation Class 계층을 두어 패더레이트 개발자들에게 보다 일반화된 HLA 서비스 사용환경을 제공해주고 동시에 반복적이고 하위수준의 RTI 프로그래밍을 자동화 할 수 있게 하였다.

• 한국시뮬레이션학회논문지
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• 제26권1호
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• pp.1-11
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• 2017

지난 수년 동안 한국군은 실제전장과 같은 엘브이시(LVC) 합성전장환경 구축을 위해 노력해 왔다. 그러한 노력에도 불구하고 성과가 극히 미미한 상태이다. 이는 주로 작전요구 및 운용개념의 결여와 아키텍처와 기반기술에 대한 정책과 체계적 접근이 미흡한데 기인한다. 더욱이 엘브이시(LVC) 기본개념과 원칙에 대한 충분한 이해 부족과 더불어 기관 간에 이해가 상충함에 기인한다. 본 연구에서 저자는 작전운용 관점보다 시스템 관점과 기술표준 관점에서 에치엘에이(HLA) 아키텍처에 기반하여 엘브이시(LVC)를 구축할 것을 제안하고 있다. 한국군이 엘브이시(LVC) 구축을 위한 접근방법으로 우선 정보중심의 통합방법을 적용하고, 아키텍처 수렴노력의 일환으로 에치엘에이/알티아이(HLA/RTI)를 기반으로 구축하며, 에치엘에이(HLA) 인증시험과 페더레이션 연동시험을 강화하고, 국제표준인 디십(DSEEP), 디마오(DMAO) 및 피트(FEAT)를 도입 적용하며, 마지막으로 에브이시(LVC) 적합성 인증시험체계를 구축하여 적용할 것을 제안하였다.

• 한국임상수의학회지
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• 제28권4호
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• pp.399-402
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• 2011

In recent years, the mesenchymal stem cells (MSC) derived from various tissues have been widely tested for developing cell therapies, tissue repair and transplantation. Although there has been much interest in the immunomodulatory properties of MSC and their immunologic reactions following autologous, allogeneic and xenogenic transplantation of MSC in vivo, up to date, the expression of immunogenic markers, such as class I and II human leukocyte antigens (HLA), after differentiation of human umbilical cord blood (hUCB)-derived MSC has been poorly investigated and require extensive in vitro and in vivo testing. In this experiment, the expression of the HLA-ABC and HLA-DR on hUCB-derived MSC have been tested by immunocytochemical staining. The undifferentiated MSC were moderately stained for HLA-ABC but very weakly for HLA-DR. In order to investigate the inhibitory effect of allogeneic lymphocytes on proliferation of MSC, the MSC were cultured in the presence or absence of peripheral allogeneic lymphocytes stimulated with concanavalin A. The allogeneic lymphocytes did not significantly inhibit MSC proliferation. We conclude that hUCB-MSC expressed moderately class I HLA antigen while almost negatively class II HLA antigen. The MSC have an immunomodulatory effect which can suppress the allogeneic response of lymphocytes. These in vitro data suggest that allogeneic MSC derived from cord blood can be useful candidate for allogeneic cell therapy and transplantation without a major risk of rejection.

• Journal of Korean Neurosurgical Society
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• 제46권6호
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• pp.558-563
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• 2009

Objective : Moyamoya disease (MMD) is an uncommon cerebrovascular disorder, characterized by progressive occlusion at the terminal portion of the internal carotid artery. Incidence of the disease is high in East Asia and familial MMD accounts for about 15% of the disease. Although the pathogenesis is unknown, association of HLA class I or II alleles with MMD has been reported with conflicting results. We investigated whether there is a difference in HLA class II association between familial and non-familial forms of the disease. Methods : A total of 70 Korean children with MMD, including 16 familial cases (10 probands), and 207 healthy controls were studied. Among familial cases, only 10 probands were used for the HLA frequency analysis. High resolution HLA-DRB1 and DQB1 genotyping was performed using polymerase chain reaction (PCR)-sequence specific oligonucleotide hybridization and PCR-single strand conformation polymorphism methods. Results : The phenotype frequencies of HLA-DRB1*1302 (70.0%) and DQB1*0609 (40.0%) were significantly increased in familial MMD compared to both controls [vs. 15.5%, corrected p ($p_c$) = 0.008, odds ratio (OR) = 12.76; vs. 4.3%, $p_c\;=\;0.02$, OR = 14.67] and non-familial MMD patients (vs. 14.8%, $p_c\;=\;0.02$, OR = 13.42; vs. 1.9%, $p_c\;=\;0.02$, OR = 35.33). The frequencies of DRB1 and DQB1 alleles in non-familial MMD patients were not significantly different from those in controls. Conclusion : Our findings suggest that the genetic polymorphism of HLA class II genes or other closely linked disease relevant gene(s) could be a genetic predisposing factor for familial MMD.

• IMMUNE NETWORK
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• 제23권6호
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• pp.44.1-44.16
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• 2023

Mesenchymal stem cells (MSCs) are effective in treating autoimmune diseases and managing various conditions, such as engraftment of allogeneic islets. Additionally, autologous and HLA-matched allogeneic MSCs can aid in the engraftment of human allogeneic kidneys with or without low doses of tacrolimus, respectively. However, HLA alloantigens are problematic because cell therapy uses more HLA-mismatched allogeneic cells than autologous for convenience and standardization. In particular, HLA-mismatched MSCs showed increased Ag-specific T/B cells and reduced viability faster than HLA-matched MSCs. In CRISPR/Cas9-based cell therapy, Cas9 induce T cell activation in the recipient's immune system. Interestingly, despite their immunogenicity being limited to the cells with foreign Ags, the accumulation of HLA alloantigen-sensitized T/B cells may lead to allograft rejection, suggesting that alloantigens may have a greater scope of adverse effects than foreign Ags. To avoid alloantigen recognition, the β2-microglobulin knockout (B2MKO) system, eliminating class-I MHC, was able to avoid rejection by alloreactive CD8 T cells compared to controls. Moreover, universal donor cells in which both B2M and Class II MHC transactivator (CIITA) were knocked out was more effective in avoiding immune rejection than single KO. However, B2MKO and CIITA KO system remain to be controlled and validated for adverse effects such as the development of tumorigenicity due to deficient Ag recognition by CD8 T and CD4 T cells, respectively. Overall, better HLA-matching or depletion of HLA alloantigens prior to cell therapy can reduce repetitive transplantation through the long-term survival of allogeneic cell therapy, which may be especially important for patients seeking allogeneic transplantation.

• 한국멀티미디어학회논문지
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• 제22권8호
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• pp.930-939
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• 2019

High-Level Architecture(HLA)/Run-Time Infrastructure(RTI) are standards for distributed simulation systems and offer a technology to interconnect them and form one single simulation system. In defense domain, M&S is the only way to prove effectiveness of weapon systems except for Live Fire Testing (LFT). This paper focuses on guided missile simulations in weapon systems for engagement analyses and proposes the integrated flying vehicle model that is based on HLA/RTI. There are a lot of missiles in real world; therefore, we should develop each missile models in M&S in order to apply battlefield scenarios. To deal with the difficulties, in this paper, firstly, I classify these missiles into three models: ballastic, cruise, and surface-to-air missile models, and then I design each missile model and integrates them into a single model. This paper also offers a case study with my integrated flying vehicle model. At the conclusion, this paper presents contributions of this paper.

• Animal cells and systems
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• 제1권1호
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• pp.129-134
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• 1997

HLA-A2 is one of the most diversified HLA-class I antigen with 17 subtypes so far identified at the molecular level. HLA-A*02 subtyping has significant implications on the tissue typing for organ and bone marrow transplantations. Recently, DNA-based typing methods have been successfully applied to the elucidation of HLA gene polymorphisms. In the present study, HLA-A*O2 genotyping was established by using nested polymerase chain reaction-sequence specific primers (PCR-SSP) and distribution of A*O2 alleles were determined in Korean individuals. Genomic DNA prepared from four B-lymphoblastoid cell lines and lymphocytes from serologically defined 48 HLA-A2 Korean individuals by phenol/chloroform extractions was typed. The results of the four B-lymphoblastoid cells were consistent with the previous data typed by PCR analysis. Five A*O2 alleles-A*0201, A*0203, A*0206, A*0207 and A*0210-were commonly observed in a total of 17 A*02 alleles. Of these, A*0207 (f=49.0%) was the most frequent allele in Korean population. A*0206 (f=28.3%) and A*0201 (f=17.0%) were also found frequently while A*0203 and A*0210 types were observed in less than 5%. In conclusion, the high level of discrimination for HLA-A*O2 alleles will prove useful and informative in the study of transplant survival, and may identify the importance of allelic differences not readily detectable by serology on host and donor compatibility.

• 한국발생생물학회:학술대회논문집
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• 한국발생생물학회 2005년도 제5회 발생공학 국제심포지엄 및 학술대회
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• pp.131-131
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• 2005