• 대한미생물학회지
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• 제19권1호
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• pp.79-83
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• 1984

To determine the existence of anti-HLA antibodies finally in 220 human placental extracts to be proved negative antiserum by previous anti-HLA A,B,C antibody screening procedure, the present study was performed by fractionation of immunoglobulins using saturated ammonium sulfate and by simple batch method on DEAE cellulose. Thereafter using known 150 T-lymphocyte panels, complement-dependent microlymphocytotoxicity test was performed to observe the existence of anti-HLA antibodies and the degree of the antibody response of the concentrates. The following results were obtained: 1. Of total 141 placental sera concentrated 45 cases(31.9%) were showed significant anti-HLA A,B,C antibody response after concentration(Excellent, 19(13.5%), Good, 3(2.1%), Weak, 23(16.3%)). 2. Anti-HLA specificities of placental sera obtained after concentration were A2, A24, B13, B27, B44, B51, CN1, C7. 3. A new type C new-1 anti-HLA antibody that is only expressed in Korean people, was obtained. 4. 79 placental sera purrified by simple batch method using DEAE cellulose were showed negative anti-HLA antibody responses.

• 한국군사과학기술학회지
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• 제9권3호
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• pp.25-32
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• 2006

Weapon systems composed of several subsystems execute various engagement missions in distributed combat environments in cooperation with a large number of subordinate/adjacent weapon systems as well as higher echelons through tactical data links. Such distributed weapon systems require distributed real-time simulation test beds to integrate and test their operational software, analyze their performance and effects of cooperated engagement, and validate their requirement specifications. These demands present significant challenges in terms of real-time constraints, time synchronization, complexity and development cost of an engagement simulation test bed, thus necessitate the use of high-performance distributed real-time simulation architectures, and modeling and simulation techniques. In this paper, in order to meet these demands, we presented a distributed real-time simulation system based on High Level Architecture(HLA) and Discrete Event System Specification(DEVS). We validated its performance by using it as a test bed for developing the Engagement Control System(ECS) of a surface-to-air missile system. The proposed technique can be employed to design a prototype or model of engagement-level distributed real-time simulation systems.

• Structural Engineering and Mechanics
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• 제82권2호
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• pp.245-258
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• 2022

Many Structural Health Monitoring (SHM) methods have been proposed for structural damage diagnosis and prognosis. However, SHM for pinched hysteretic structures can be problematic due to the high level of nonlinearity. The model-free hysteresis loop analysis (HLA) has displayed notable robustness and accuracy in identifying damage for full-scaled and scaled test buildings. In this paper, the performance of HLA is compared with seven other SHM methods in identifying lateral elastic stiffness for a six-story numerical building with highly nonlinear pinching behavior. Two successive earthquakes are employed to compare the accuracy and consistency of methods within and between events. Robustness is assessed across sampling rates 50-1000 Hz in noise-free condition and then assessed with 10% root mean square (RMS) noise added to responses at 250 Hz sampling rate. Results confirm HLA is the most robust method to sampling rate and noise. HLA preserves high accuracy even when the sampling rate drops to 50 Hz, where the performance of other methods deteriorates considerably. In noisy conditions, the maximum absolute estimation error is less than 4% for HLA. The overall results show HLA has high robustness and accuracy for an extremely nonlinear, but realistic case compared to a range of leading and recent model-based and model-free methods.

• Asian Pacific Journal of Cancer Prevention
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• 제16권14호
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• pp.6155-6158
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• 2015

Background: The human leukocyte antigen-G (HLA-G) gene is highly expressed in cancer pathologies and is one strategy used by tumor cells to escape immune surveillance. A 14-bp insertion/deletion (InDel) polymorphism of the HLA-G gene has been suggested to be associated with HLA-G mRNA stability and the expression of HLA-G. The aim of present study was to assess any genetic association between this polymorphism and breast cancer among Iranian-Azeri women. Materials and Methods: In this study 227 women affected with breast cancer, in addition to 255 age-sex and ethnically matched healthy individuals as the control group, participated. Genotyping was performed using polymerase chain reaction and electrophoresis assays. The data were compiled according to the genotype and allele frequencies, compared using the Chi-square test. Statistical significance was set at P<0.05. Results: In this case-control study, no significant difference was found between the case and control groups at allelic and genotype levels, although there is a slightly higher allele frequency of HLA-G 14bp deletion in breast cancer affected group. However,when the stage I subgroup was compared with stage II plus stage III subgroup of affected breast cancer, a significant difference was seen with the 14 bp deletion allele frequency. The stage II-III subgroup patients had higher frequency of deletion allele (57.4% vs 45.8%) than stage I cases (${\chi}^2=4.16$, p-value=0.041). Conclusions: Our data support a possible action of HLA-G 14bp InDel polymorphism as a potential genetic risk factor for progression of breast cancer. This finding highlights the necessity of future studies of this gene to establish the exact role of HLA-G in progression steps of breast cancer.

• 대한임상검사과학회지
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• 제38권3호
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• pp.189-195
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• 2006

Behcet's disease (BD) is a chronic, multisystemic disorder which is more frequently seen in the Mediterranean basin, Middle East, and Far East. The causes and pathogenesis of BD are unknown although many possibilities are being investigated. The diagnosis of BD is based on clinical manifestations because there are no pathognomonic laboratory tests. So, the purpose of this study was to examine the prevalence of HLA-B51 in patients with BD. We used the whole blood of 33 patients diagnosed with BD at Chosun University Hospital from August 2003 to January 2006. For the HLA-B51 test, we extracted the DNA from the whole blood of 33 BD patients, and we investigated it through the nested PCR method. Data were analyzed using the SPSS/PC 10.0. The frequencies of gender of the 33 cases diagnosed as BD were male 13 (39.4%) and female 20 (60.6%). The frequencies of age group of the 33 cases diagnosed as BD were 20 yrs 8 (24.2%), 30 yrs 12 (36.4%), 40 yrs 8 (24.2%), 50 yrs 1 (3.0%), and 60 yrs and 70 yrs 2 (6.1%), respectively. The frequencies of HLA-B51 of the 33 cases diagnosed as BD were HLA-B51-negative 18 (54.5%) and HLA-B51-positive 15 (45.5%). In conclusion, BD occurred more often in women than men (1: 1.53), and the mean age of the BD patients was 39.8 years old. HLA-B51 was positive in 45.5% of patients with BD, and was statistically significant in age (p<0.05).

• Genomics & Informatics
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• 제13권4호
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• pp.126-131
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• 2015

Fulminant type 1 diabetes (T1DM) is a distinct subtype of T1DM that is characterized by rapid onset hyperglycemia, ketoacidosis, absolute insulin deficiency, and near normal levels of glycated hemoglobin at initial presentation. Although it has been reported that class II human leukocyte antigen (HLA) genotype is associated with fulminant T1DM, the genetic predisposition is not fully understood. In this study we investigated the HLA genotype and haplotype in 11 Korean cases of fulminant T1DM using imputation of whole exome sequencing data and compared its frequencies with 413 participants of the Korean Reference Panel. The $HLA-DRB1^*04:05-HLA-DQB1^*04:01$ haplotype was significantly associated with increased risk of fulminant T1DM in Fisher's exact test (odds ratio [OR], 4.11; 95% confidence interval [CI], 1.56 to 10.86; p = 0.009). A histidine residue at $HLA-DR{\beta}1$ position 13 was marginally associated with increased risk of fulminant T1DM (OR, 2.45; 95% CI, 1.01 to 5.94; p = 0.054). Although we had limited statistical power, we provide evidence that HLA haplotype and amino acid change can be a genetic risk factor of fulminant T1DM in Koreans. Further large-scale research is required to confirm these findings.

• BMB Reports
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• 제38권3호
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• pp.350-353
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• 2005

Alanine at residue 73 (Ala-73) and aspartate at residue 9 (Asp-9) are characteristic to both Cw6 and Cw7 alleles of HLA-C gene and have been suggested as possible markers for psoriasis vulgaris (PsV). However, the results from various ethnic groups/populations are contradictory and inconclusive. In this study, an attempt has been made to examine the association between HLA-C (Ala-73 and Asp-9) and susceptibility to PsV among Saudi patients. Genomic DNA was extracted from 25 Saudi PsV patients and 75 control subjects. Polymerase chain reaction (PCR) was performed to amplify HLA-C sequences using earlier reported primers, C133P and C243PR. Sequence-specific primers were used to specifically detect nucleotide coding for Ala-73 and Asp-9 in all the subjects. The results showed significantly higher frequency of Asp-9 (84.0% versus 61.3%) in PsV patients as compared to controls (p < 0.05, 2-tailed Fisher's exact test). The frequencies of Ala-73 among PsV patients (92%) and controls (88%) did not differ significantly.

• IMMUNE NETWORK
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• 제2권4호
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• pp.227-232
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• 2002

Background: Systemic lupus erythematosus (SLE) is a complex autoimmune disease characterized by diverse clinical manifestations and autoantibody production, which is known to be strongly influenced by genetic factors. Previous studies have revealed the associations of SLE with HLA class II alleles and antiphospholipid antibody system (anticardiolipin antibody (aCL) and anti-${\beta}_2$ glycoprotein I antibody (anti-${\beta}_2$ GPI)). Therefore, we studied the associations of HLA class II alleles with SLE and antiphospholipid antibody system. Methods: The genotyping for HLA-DRB1 and DQB1 alleles were performed in 61 SLE patients and 100 controls by the polymerase chain reaction (PCR)-sequence specific oligonucleotide probe method. ELISA tests for aCL and anti-${\beta}_2$ GPI were performed in 39 of the 61 SLE patients. The results were evaluated statistically by Chi-square test. Results: The frequencies of the HLA-$DRB1^*15$ and $DQB1^*06$ in SLE patients were significantly higher than those in controls. HLA-$DRB1^*12$ was significantly lower in SLE patients than controls. Nine of 39 patients were positive for aCL (IgG) and three were positive for aCL (IgM). One of 39 patients were positive for anti-${\beta}_2$ GPI (IgG) and none of them positive for anti-${\beta}_2$ GPI (IgM). Association of aCL with HLA class II alleles was not observed in our study. Conclusion: According to our results, it was found that HLA-$DRB1^*15$ and $DQB1^*06$ were associated with genetic susceptiblility and $DRB1^*12$ was associated with resistance to SLE in Korean population. No Association of aCL with HLA class II alleles was observed and the positive rate for anti-${\beta}_2$ GPI was very low.

• 정보처리학회논문지D
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• 제12D권5호
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• pp.709-718
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• 2005

예측 불가능한 미래 전략 환경에 부합하고, 보다 경제적이며 과학적인 수단으로 무기체계개발을 수행하기 위해 모델링 및 시뮬레이션 기술의 적용이 확대되고 있다. 본 연구는 모델링 및 시뮬레이션 표준연동구조인 HLA(High Level Architecture)및 TCP/IP를 지원하고, 실시간 분산 시뮬레이션을 지원하는 소프트웨어 프레임워크인 ADSF(Air Defense Simulation Framework)를 개발한 경험을 기술하고 있다. 개발된 ADSF는 대공유도무기 M-SAM(Medium Range Surface to Air Missile) 체계시뮬레이터에 적용하였으며, 위성항법장치(GPS : Global Positioning System) 시계를 사용하여 요구조건에 만족하는 시험결과를 산출하였다. 그 결과 ADSF는 HLA를 지원하고 실무기 체계의 통신 프로토콜인 TCP/IP를 지원하는 통신미들웨어가 만들어지고, 실시간 분산 시뮬레이션의 핵심 기술인 정밀모사를 지원하는 시뮬레이션 엔진도 갖추게 되었다. 대공유도무기 체계시뮬레이터는 다수의 구성요소로 이루어진 실시간 분산 시뮬레이션 시스템이므로 실시간 분산 시뮬레이션을 지원해 주는 시뮬레이션 엔진 개발이 요구되었다.

• 한국시뮬레이션학회논문지
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• 제16권3호
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• pp.11-18
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• 2007

최근 무기체계는 성능이 다양하고 복잡해지고 있으며 에 따라 개발 위험 요소가 증대하고 있다. 이에 따라 무기체계 회득 비용의 감소 및 획득 기간의 감소 요구가 증대되고 있다. 이와 같은 획득 환경 아래에서 무기체계를 보다 효율적으로 개발하기 위하여 무기체계 소요제기에서부터 국방 M&S 기법을 적용할 필요성이 대두되면서 M&S 체계를 개발하여 적용하고 있다. 분산 환경하에서 모델링 및 시뮬레이션에 대한 중요성이 강조되면서 시뮬레이션 모델들의 상호운용성, 재사용성 및 확장성을 향상시키고 데이터 교환 및 시간 진행을 조절하는 등의 장점을 지닌 HLA, SEDRIS 등의 M&S 표준이 개발되었고 상호운용성을 개선시킬 수 있도록 체계공학적인 절차인 FEDEP(Federation Development & Execution Process)을 개발하고 이 절차의 사용을 권장하고 있다. 본 논문에서는 5단계의 표현을 기준으로 사용하여 해양무기체계 시뮬레이션 기반 설계 기술 구축 연구의 해양무기체계 Test Bed를 개발하기 위한 Prototype으로 수중 교전 시뮬레이터(Underwater Engagement Simulation:UNES)를 구성함에 있어 통합 아키텍처로 HLA를 채택하고 있다. 따라서, 미래의 확장성을 위하여 FEDEP에 따라 시뮬레이터를 개발하였으며 UNES 개발에 FEDEP를 적용하는 과정을 기술하고 그 과정 동안에 확인하여야 할 사항에 대하여 정리하였다.