• The Journal of Korean Society of Virology
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• v.26 no.1
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• pp.121-129
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• 1996

Multiplication of Herpes simplex virus type-1 was observed by electronmicroscopy, a gene library of the genome was constructed and thymidine kinase gene was cloned. Vero cells infected with the virus were lysed 48 h p.j. and multinucleated giant cells were observed approximately at 72 h p.i. The nucleocapsids were observed in nuclei and cytoplasm, and the assembled nucleocapsids were budded out through the vacuole and cytoplasmic membranes, and then virions were released from the cells. HSV-1 genome DNA was digested with BamHI and BglII enzymes and then the gene library of the genome fragments were constructed. The BamHI cleaved the genome DNA into twenty-seven fragments in the range of 1.1 - 14 kb, and BglII cleaved the genome DNA into sixteen fragments in the range of $4.5{\sim}20.1\;kb$. The pHLA-12 and pHLB-4 recombinant plasmids were contained TK gene by Southern blot analysis. The molecular sizes of the fragments which contained the TK gene were 3.74 in pHLA-12 and 6.41kb in pHLB-4 recombinant plasmid, respectively.

• Journal of the Korea Society for Simulation
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• v.17 no.1
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• pp.33-42
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• 2008

In general, simulation is to predict or evaluate some systems that are hard to be executed in real world, and so usually the target systems to be modeled are large and complex. Trying to observe the dynamics of the systems results in similar level of animation complexity, the model and animation has the same complexity as the system. Trying to display all the graphic objects representing the dynamics of the models being simulated, however, causes the distraction of focus, which results in solving the above listed problems difficult. The redundant graphic objects also increase the computer computation overhead. To solve the problem, a research about a hierarchical animation environment has been proposed a few years ago. In the research, the users can have better focus on the dynamics of system components by selectively choosing the hierarchical level and components within a level of the hierarchically structured model. However, the research has not a modeling methodology for modelers to describe systematically animation part corresponding to dynamics of simulation in a model. This research has defined the modeling methodology of DESHA and defined DESHA-C++, improving the previous research output, as an execution environment of DESHA models. In addition, to use hierarchical animation environment in various problems, this research proposed and developed the distributed simulation modeling environment that connects DESHA environment and HLA.

• Korean Journal of Microbiology
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• v.47 no.4
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• pp.342-347
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• 2011

T cells play a key role in viral infection. However, in patients with chronic hepatitis C virus (HCV) infection, HCV-specific T cells are dysfunctional and impaired in the liver, which is the primary site for HCV replication. There are multiple potential mechanisms for HCV-specific T cell dysfunction including induction of immune inhibitory pathways (program death-1; PD-1, cytotoxic t lymphocyte associated antigen-4; CTLA-4) and immune tolerance induced specific for the liver. However, the interaction between hepatocytes and HCV-specific CD8 T cells has not clearly established. In this study, we confirmed huh (human hepatoma) 7.5 cells expressing HLA (human leukocyte antigen) A2 presented antigen to activate HCV-specific CD8 T cells in HLA A2-restricted manner and expression of PD-L (program death ligand) 1 on huh7.5 cells reduced HCV-specific CD8 T cell activation, suggesting an immune modulatory activity. Loss of HCV-specific tetramer responses following antigenic stimulation correlated with increased caspase-3 activity. In addition, PD-L1 on huh7.5 cells rescued HCV-specific CD8 T cells from apoptosis. Our results suggest that the interaction between PD-L1 and PD-1 can recover the function of HCV-specific CD8 T cells in the liver, which could be applied in therapy of HCV chronic infection.

• Journal of the Korea Society for Simulation
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• v.19 no.4
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• pp.291-299
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• 2010

As recent advances in science, technology and performance requirements of the weapons system are getting highly diversified and complex, the performance requirements also get stringent and strict. Moreover, the weapons system should be intimately connected with other systems such as watchdog system, command and control system, C4I system, etc. However, a tremendous amount of time, cost and risk being spent to acquire new weapons system, and not being diminished compared to the rapid pace of its development speed. Defense Modeling and Simulation(M&S) comes into the spotlight as an alternative to overcoming these difficulties as well as constraints. In this paper, we propose the development process of virtual test framework based on the synthetic environment as a tool to analyze the effectiveness of the weapons system of underwater engagement model. To prove the proposed concept, we develop the test-bed of virtual test using Delta3D simulation engine, which is open source S/W. We also design the High Level Architecture and Real-time Infrastructure(HLA/RTI) based Federation for the interoperation with heterogeneous simulators. The significance of the study entails (1)the rapid and easy development of simulation tools that are customized for the Korean Theater of War; (2)the federation of environmental entities and the moving equations of the combat entities to manifest a realistic simulation.

• 대한두경부종양학회:학술대회논문집
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• 1995.11a
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• pp.212.1-212.1
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• 1995

• 대한두경부종양학회:학술대회논문집
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• 1995.11a
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• pp.199.1-199.1
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• 1995

• Proceedings of the Korean Information Science Society Conference
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• 2000.04a
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• pp.247-249
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• 2000

네트워크 인프라와 개인 PC 의 성능 향상으로, 실세계와 흡사한 가상세계가 점차 활성화되는 추세이다. 네트워크에 기반한 가상 환경이 갖는 가장 큰 장점은, 물리적으로 멀리 떨어져 있는 여러 사용자들을 하나의 가상공간에 있는 듯한 착각(illusion)을 주고, 실시간으로 상호작용이 가능하다는 것이다. 본 논문에서는 이러한 networked Virtual Environment(이하 net- VE)의 구성에 고려되어 지는 네트워크 구조와 DIS, HLA, vrtp등의 프로토콜을 살펴보고, 기존의 군사용 그리고 대학에서 구성된 net-VE 환경들에 대한 일반적인 특성들을 정리해본다. 그리고 현재 국내에서 서비스 되고 있는 net-VE 환경을 살펴본다.

• Proceedings of the Korea Society for Simulation Conference
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• 1998.03a
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• pp.38-42
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• 1998

본 논문에서는 DEVS 형식론과 High Level Architecture에 기반을 둔 이 기종 시뮬레이션 환경의 구축에 대해 기술한다. DEVS 형식론은 여러 가지 방법으로 기술된 모델들을 동일한 형식론으로 간주하기 위해 사용되었다. 즉, 이산사건 모델링을 위한 세가지 세계관(world view)으로 기술된 시뮬레이션 모델들을 DEVS 형식론으로의 변환을 통해 전체적으로는 DEVS 형식론만을 사용한 것과 동일한 형태로 표현되도록 하였다. High Level Architecture는 시뮬레이션 수행시의 상호 연동성을 보장하기 위해 사용되었다. 이때, DEVS 형식론과 High Level Architecture에서의 시뮬레이션 시간 진행 방법이 다르기 때문에 이의 해결을 Synchronizer, EOS 방법을 제안하였다.

• Defense and Technology
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• no.3 s.277
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• pp.10-17
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• 2002

현재 우리 국방예산은 점점 감소되고 있으며 군구조 일부 통합되어 축소 지향적으로 가고 있는 것으로 볼 때 국방자원의 감소는 시대적인 추세라고 볼 수 있다. 따라서 HLA를 기반으로한 무기체계획득, 교육/훈련체계의 획기적인 개선의 필요성은 크게 증가되고 있다. 더구나, 최근 인공지능기술을 포함한 각종 정보 통신기술의 급격한 발전에 따라 이러한 시뮬레이션/시뮬레이터 개발비용이 감소되고 있는 것은 이런 분야에 대한 방위산업의 활성화에 크게 도움을 줄 수 있을 것으로 판단된다.

• Clinical and Experimental Pediatrics
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• v.46 no.11
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• pp.1124-1127
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• 2003

We evaluated the clinical and laboratory characteristics of five children with Kawasaki disease who had showed arthritis after responding to intravenous immunoglobulin(IVIG) treatment. Age distribution was between 13 months and six years of age(mean $3.2{\pm}1.6$ years). There were two males and three females. Arthritis occurred when acute symptoms were subsiding, with the average onset on day $5.8{\pm}1.8$ after final IVIG treatment. Arthritis was pauciarticular in three, and polyarticular in two. Regarding laboratory findings, one child was positive in rhematoid factor and changed to negative after two months. Three patients were examined for HLA B27 and all showed negative results. High dose aspirin(two cases), anti-inflammatory drug(ibprofen, three cases), and corticosteroids(methyprednisolon pulse therapy, one case) were used for this type of arthritis. Symptoms and signs of arthritis in all patients were improved by these therapies. There was no relapse or complications within six months. Arthritis after responding to IVIG therapy was rarely observed in children with Kawasaki disease. This type of arthritis responded well to anti-inflammatory drugs including corticosteroids, and showed no relapses.