• The Bulletin of The Korean Astronomical Society
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• v.36 no.2
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• pp.143.1-143.1
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• 2011

Deep Ca,b,y images obtained from the CTIO 4m Blaco telescope are used to investigate the multiple stellar populations of red giant branch (RGB) and sub-giant branch (SGB) in Galactic globular clusters NGC 6656 and NGC 6723. For NGC 6656, confirming the result of Lee et al. (2009), we find two discrete populations of the RGB stars of which mean color separation is about 0.2 mag in hk[=(Ca-b)-(b-y)] index. Furthermore, we also find the bimodel distribution of the SGB stars in (hk, y) color-magnitude diagram. A new finding is that the (hk, y) color-magnitude diagram of NGC 6723 shows two distinct RGB stars with different calcium abundances of which mean color separation is about 0.12 mag in hk index. This multiple stellar feature has not been observed in previous observation, suggesting that NGC 6723 may also be a possible relic of dwarf galaxies that merged into the Milky Way in the past. Thus our result adds further constraints to the merging scenario of the Galaxy formation. Unfortunately, the split of SGB stars in NGC 6723 is not obvious. We will present some statistical results to compare properties of two populations in two clusters.

• Journal of the Korean institute of surface engineering
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• v.34 no.2
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• pp.125-134
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• 2001

($Ti_{1-x}$ $Cr_{x}$ )N coatings were deposited by an ion-plating method in a reactor with two separate metal sources, Ti and Cr. Ti was evaporated using an electron beam, while Cr evaporation was carried out by resistant heating. The Ti and Cr concentrations in the coatings were controlled by the Ti and Cr evaporation ratio. The coating hardness increased with increasing the Cr content(x) and showed a maximum value of 6,000 HK at around x=0.8. The critical load of the coatings, measured by the scratch test, was around 30 N. The wear resistance properties of the ($Ti_{1-x}$$Cr_{ x}$)N coatings were evaluated using a CSEM pin-on-disk type tribometer. A Cr-steel ball as well as a SiC ball, which had hardness values of 590 HK and 2,600 HK respectively, were used as the pin. After the wear test, the surface morphology, roughness and the concentration of the coatings were investigated, with the main focus being on the effect of wear debris and the transferred layer on the wear behavior.

• Korean Journal of Crystallography
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• v.9 no.2
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• pp.149-152
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• 1998

The accurate expression of the anisotropic thermal parameters is either exp (-2$π^{2}$ < $h^{2}$ $\frac{$u_x^2$}{$a^{2}$}$ + $k^{2}$ $\frac{$u_y^2$}{$b^{2}$}$ + $l^{2}$ $\frac{$u_z^2$}{$c^{2}$}$ + 2hk $\frac{$u_{x}}{a}$ $\frac{$u_{y}}{b}$ 2hl $\frac{$u_{x}}{a}$ $\frac{$u_{z}}{c}$ 2kl $\frac{$u_{y}}{b}$ $\frac{$u_{z}}{c}$ > ) with the small displacements Ux, Uy, uz, in absolute measure or exp (-2$π^{2}$ < $h^{2}$ $u_x^2$ + $k^{2}$ $u_y^{2}$ + $l^{2}$ $u_z^{2}$ + 2hk$u_{x}$ $u_{y}$ + 2hl$u_{x}$$u_{z}$ + 2kl$u_{y}$ $u_{z}$ > ) with the small displacements Ux, Uy, Uz in fractional measure.

• The Bulletin of The Korean Astronomical Society
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• v.37 no.2
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• pp.145.2-145.2
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• 2012

NGC 6273은 우리은하 중앙팽대부 영역에 위치하고 수평계열이 확장되어 있는 구상성단으로 초신성에 의해 중원소 함량이 증가된 2세대 종족의 존재가 의심되는 성단 중 하나이다. 이를 보다 면밀히 조사하고자 2011년 6월, 칠레에 위치한 Las Campanas Observatory의 2.5m Du Pont 망원경과 2K CCD를 이용하여 Ca, b, 그리고 y 필터로 측광 관측을 수행하였다. 중원소 함량에 민감하게 반응하는 hk (hk=(Ca-b)-(b-y)) 색지수로 색등급도를 그려본 결과 적색거성계열이 관측오차의 8배 이상으로 매우 넓게 퍼져있는 것을 발견하였다. 이러한 현상이 중원소 함량 차이로 인한 것인지, 아니면 성간소광효과나 배경별에 의한 영향인지를 분석하였고, 그 결과 중원소 함량의 차이가 있는 다중종족의 존재 가능성을 배재할 수 없음을 확인하였다. 이를 확인하기 위한 저해상도 분광관측이 진행되고 있으며, 이의 예비분석 결과를 아울러 발표할 예정이다.

• Korean Journal of Ichthyology
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• v.18 no.2
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• pp.97-106
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• 2006

In the investigation of geographic variations on the Zacco koreanus distributed widely in southern Korean Peninsula, it was found that there were distinct differences in the nuptial coloration among geographic populations. These color variations were presence of red band on anterior margin of pectoral fin and color patterns of dorsal fin. On the basis of those color variations three color types were recognized in Z. koreanus, that is, HK, NS and NE types. HK types were distributed mainly in Han and Geum River, NS types in Nakdong and Seomjin River, and NE types in Nakdong River and small streams on the east coast. It was noted ecologically and taxonomically that these three types were well separated geographically and two types of them, i.e., NS and NE types cohabit in the Nakdong River and shown a tendency of microhabitat segregation between them.

• Journal of Ginseng Research
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• v.43 no.1
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• pp.135-142
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• 2019

Background: Panax ginseng Meyer, known as Korean Red Ginseng (KRG), is one of the important age-old traditional herbs used in boosting libido and improving male fertility. In this study, the effects of Rg3-enriched KRG extract (KGC04P) on heat stress-induced testicular damage in experimental rats was evaluated. Methods: Male rats (Sprague-Dawley) were divided into four groups (n = 10): normal control (NC), heat-stressed control (HC), heat-stressed plus KGC04P-100 mg/kg (HK100), and heat-stressed plus KGC04P-200 mg/kg (HK200) groups. Starting 1 week prior to heat stress, animals were administered orally with KGC04P (100 and 200 mg/kg) mixed with a regular pellet diet and continued for 25 weeks. Heat stress was induced to HC, HK100, and HK200 groups by intermittently exposing the animals to high temperatures ($32{\pm}1^{\circ}C$, 2 h/day). After 6 months, animals were euthanized under general anesthesia with carbon dioxide and evaluated for various parameters in serum and testicular tissue by using Western blotting, biochemical kits, and reverse transcription-polymerase chain reaction. Results: Significant (p < 0.05) alterations in several parameters, such as body/organ weight, sperm kinematics, and lipid metabolism marker levels, in the serum and testis of rats were observed. Further, the expression of testicular antioxidant enzymes, inflammatory cytokines, sex hormonal receptors, and spermatogenesis-related genes were also affected significantly (p < 0.05) in the heat-stressed group. However, KGC04P prevented the heat stress-induced changes in rats significantly (p < 0.05) at both concentrations. Conclusion: KGC04P attenuated heat stress-induced testicular damage by a multifunctional approach and can be developed as an excellent therapeutic agent for hyperthermia-mediated male infertility.

• IMMUNE NETWORK
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• v.13 no.5
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• pp.199-204
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• 2013

Syntenin is an adaptor molecule containing 2 PDZ domains which mediate molecular interactions with diverse integral or cytoplasmic proteins. Most of the results on the biological function of syntenin were obtained from studies with malignant cells, necessitating exploration into the role of syntenin in normal cells. To understand its role in normal cells, we investigated expression and function of syntenin in human lymphoid tissue and cells in situ and in vitro. Syntenin expression was denser in the germinal center than in the extrafollicular area. Inside the germinal center, syntenin expression was obvious in follicular dendritic cells (FDCs). Flow cytometric analysis with isolated cells confirmed a weak expression of syntenin in T and B cells and a strong expression in FDCs. In FDC-like cells, HK cells, most syntenin proteins were found in the cytoplasm compared to weak expression in the nucleus. To study the function of syntenin in FDC, we examined its role in the focal adhesion of HK cells by depleting syntenin by siRNA technology. Knockdown of syntenin markedly impaired focal adhesion kinase phosphorylation in HK cells. These results suggest that syntenin may play an important role in normal physiology as well as in cancer pathology.

• Journal of Life Science
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• v.21 no.9
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• pp.1214-1218
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• 2011

We investigated the fruits of Rubus crataegifolius Bge, a plant which has been traditionally used in Korea in phytotherapy, to describe antioxidant materials from plant sources. R. crataegifolius fruits were extracted with methanol and further fractionated into n-hexane, diethyl ether, and ethyl acetate. The antioxidant activity of each fraction and the residue was assessed using a 1,1-diphenyl-2-picrylhydrazyl (DPPH), $H_2O_2$ radical scavenging method, and their cytotoxicity on human primary kerationcyte (HK) was determined by an MTS assay. The R. crataegifolius fruit methanol extract showed strong antioxidant activity (75.04%, 50%) compared with vitamin C (79.9%, 54.1%) by the DPPH, and $H_2O_2$ method, respectively. The measured activity from the subsequent extracts of the methanol extract were 20.3% for n-hexane fraction (HF), 68.8% for diethyl ether fraction (DF), 67.1% for ethyl acetate fraction (EF), and 67.1% for the residue fraction (RE) by DPPH and 2.2% for HF, 1.6% for DF, 10% for EF, and 50% for the RE by $H_2O_2$ assay. An oxidative stress model of HK was established under a suitable concentration (1 mM). The cell viability of the RE treated group increased and the percentage of apoptotic cells decreased at concentrations of 0.005-0.02% RE compared with the $H_2O_2$ treated group. Fruit extracts of the medicinal plant R. crataegifolius showed potent antioxidant activity and the ability to relieve cell damage from $H_2O_2$ induced injury to HK.

• Korean Journal of Veterinary Research
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• v.43 no.3
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• pp.439-448
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• 2003

The VP2 gene of infectious bursal disease virus (IBDV) Chinju which was previously detected in Chinju, Korea was cloned and sequenced to establish the information for the development of genetically engineered vaccines and diagnostic reagents against IBDV. The nucleotide sequence of the entire Chinju VP2 gene consisted of 1,356 bases long encoding 452 amino acids in a single open reading frame (ORF). It consisted of 368 adenine (27.1%), 363 cytosine (26.8%), 339 guanine (25.0%) and 286 thymine (21.1%) residues. The predicted $M_r$ of the Chinju VP2 protein was 48 kDa, and the protein contained 13 phosphorylation sites by protein kinase C, casein kinase II or tyrosine kinase, whereas 3 asparagine-linked glycosylation sites were recognized. The nucleotide sequence of Chinju VP2 ORF had a very close phylogenetic relationship with 98-99% homology to that of the very virulent IBDVs (vvIBDVs) HK46, OKYM, D6948, UK661, UPM97/61 and BD3/99. Also, the Chinju VP2 protein revealed a very close phylogenetic relationship with 99-100% homology to that of these vvIBDVs. The Chinju VP2 protein had 100% amino acid identity in the variable region of residues 206-360 with that of the D6948, HK46, OKYM and UK661, as well as 100% identity in two hypervariable regions of residues 212-224 and 314-324 with those of the D6948, HK46, OKYM, UK661, UPM97/61 and BD3/99. The amino acid sequence of the chinju VP2 protein contained a serine-rich heptapeptide of SWSASGS as in these vvIBDVs.

• Toxicological Research
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• v.34 no.2
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• pp.133-141
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• 2018

Anti-cancer drugs such as cisplatin and doxorubicin are effectively used more than radiotherapy. Cisplatin is a chemotherapeutic drug, used for treatment of various forms of cancer. However, it has side effects such as ototoxicity and nephrotoxicity. Cisplatin-induced nephrotoxicity increases tubular damage and renal dysfunction. Consequently, we investigated the beneficial effect of cynaroside on cisplatin-induced kidney injury using HK-2 cell (human proximal tubule cell line) and an animal model. Results indicated that $10{\mu}M$ cynaroside diminished cisplatin-induced apoptosis, mitochondrial dysfunction and caspase-3 activation, cisplatin-induced upregulation of caspase-3/MST-1 pathway decreased by treatment of cynaroside in HK-2 cells. To confirm the effect of cynaroside on cisplatin-induced kidney injury in vivo, we used cisplatin exposure animal model (20 mg/kg, balb/c mice, i.p., once a day for 3 days). Renal dysfunction, tubular damage and neutrophilia induced by cisplatin injection were decreased by cynaroside (10 mg/kg, i.p., once a day for 3 days). Results indicated that cynaroside decreased cisplatin-induced kidney injury in vitro and in vivo, and it could be used for improving cisplatin-induced side effects. However, further experiments are required regarding toxicity by high dose cynaroside and caspase-3/MST-1-linked signal transduction in the animal model.