• 대한바이러스학회지
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• 제28권4호
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• pp.359-368
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• 1998

Cytomegalovirus is the most common cause of life-threatening viral infection in HIV-infected patients. This study was done prospectively to investigate the incidence of CMV infection according to the decrease of CD4+ T cell count (CD4+) in Korean AIDS patients. Thirty-nine HIV-infected patients diagnosed before 1994 were followed for regular immunological monitoring. We have used urine shell vial method for the CMV detection from 1994 and have also checked clinical findings. Positive urine culture rate definitely depended on the CD4+ as follows; 45%, 22%, 17%, 11% and 0%, CD4+ <50, 50-100, 100-200, 200-500 and >500, respectively. Except culture positive 2 patients with CD4+ of $200{\sim}300/{\mu}l$, all eight culture positive patients with CD4+ less than $200/{\mu}l$ showed CMV related diseases on or before urine culture. But, we could not get a positive culture for a late AIDS patient with vision loss. With ganciclovir therapy, all culture results were at least negative just after or on late of first 14 days-ganciclovir infusion-course. These data suggest that the incidence of CMV disease in Korean AIDS patients is very high, and early diagnosis and treatment for CMV diseases is required for the prevention of life threatening results.

• 한국임상약학회지
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• 제22권2호
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• pp.95-102
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• 2012

HIV 치료를 위한 강력한 항바이러스 약물요법이 널리 사용됨에 따라 HIV에 감염된 상태에서 신장질환 발생 위험성을 지닌 채 오랜 기간 생존하는 환자들이 증가하고 있다. 본 연구는 국립중앙의료원 감염병 센터를 내원한 만18세 이상의 HIV 감염 환자를 대상으로 HIV 감염 환자에게 신기능 장애를 유발하는 위험인자를 평가하고자 환자군 대조군 연구를 후향적으로 실시하였다. 2006년 1월부터 2011년 3월까지 5년 3개월 동안 신기능이 저하된 모든 HIV 감염 환자를 환자군으로 하며, 정상 신기능을 가진 HIV 감염 환자들 중 대조군을 무작위로 선정하여 환자군과 대조군을 1:2의 비율로 하였다. 환자군과 대조군을 비교해 만성신질환을 유발하는 위험인자를 평가하기 위한 분석변수로 성별, 연령, CD4+ 세포수, 혈중 바이러스 수, HAART 56일 이상 여부, 당뇨병과 C형 간염을 선정하였다. 또한 추가적으로 개별 antiretroviral 약물들 사용과 신기능이 얼마나 관련되어 있는지 알아보기 위해 각각의 약물과 eGFR의 상관관계를 분석하였다. 환자군은 CD4+ 세포수가 < $200{\times}10^6$ cells/l 인 군이 7.7배(OR: 7.7; 95% CI, 1.8-32.9) 단백뇨가 있는 환자의 경우 7.8배(OR: 7.8; 95% CI, 1.6-37.8) 더 유의하게 만성신질환 발생위험이 높았다. 개별 antiretroviral 약물들과 eGFR의 상관관계를 분석한 결과, lamivudine 이 eGFR 과 약한 음적 상관관계를 보이는 것으로 나타났으며(r = -.211, p < .05), 다른 약물들의 경우 통계적으로 유의한 값을 보이지 않았다. 이번 환자군-대조군 연구는 HIV 감염 환자들이 만성 신질환으로 발전하는데 여러 인자들의 역할에 대해 평가하고자 하였다. 여러 변수들을 평가해 본 결과, 만성 신질환 환자들의 경우 CD4+ 세포수가 < $200{\times}10^6$ cells/l 이거나 단백뇨를 동반한 경우가 통계적으로 유의하게 많았다.

• 보건교육건강증진학회지
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• 제11권2호
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• pp.33-47
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• 1994

AIDS preventional efforts need to be guided by well trained health care personnel especially by school nurses for the adolescents. This study was designed to get basic data about AIDS educational program development for school nurses. The objectives of this study were to 1) identify AIDS knowledg of school nurses 2) identify AIDS attitudes of school nurses and 3) identify association between AIDS knowledge and sociodemographic characteristics. Data were collected from 173 subjects in Chonbuk province. Self-reporting questionnaire were administered during the period from 1st of June to 30th of June, 1994. AIDS related knowledge was measured by using 44 questions on cause(3 items), testing(3 items), mode of transmission(15 items), clinical manifestations(5 items), treatment(3 items), prevention(5 items), complication(4 items), infection control(3 items) and Using resources(3 items). AIDS related attitudes were measured by five point Likert scales using 13 questions on perceived threats from AIDS crisis (4 items), perceived severity to AIDS(2 items), perceived needs about psychosocial care for HIV infected patients(3 items) and perceived educational needs of AIDS(4 items). The collected data were analyzed by SPSS/PC/sup +/, using percentages, Mean and S.D. descriptive purpose and t-test or F for comparing the variables. The major findings were as follows: 1. Respondents ranged in knowledge of AIDS between 0 and 44 with the 33.79 mean score. Percentage of correctly answered respondents to each categories - mode of transmission : 87.0% - clinical manifestation : 85.0% - cause : 82.5% - prevention : 81.5% - treatment : 76.1% - infection control : 72.8% - testing of HIV infection 71.8% - using community resourses : 50.9% - complication : 45.8% 2. The respondents' attitudes of AIDS The majority(70%) showed higher perceived threat The majority(91.3%) showed higher perceived severity to AIDS crisis As a whole, perceived needs about psychosocial care for HIV infected patients negative The majority (over 96.5%) showed highly perceived educational needs of AIDS. 3. The relationship between AIDS knowledge and each of those general character. AIDS knowledge shows significant difference with age(F=3.50, p<.016), years of professional experience(F=4.14, p<.007) and received lecture about AIDS(F=4.54, p<.000). There was no significant difference between AIDS knowledge and job satisfaction.

• BMB Reports
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• 제31권5호
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• pp.432-443
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• 1998

The poliovirus Sabin 1 strain has features that make it a particularly attractive live recombinant mucosal vaccine vehicle. Sabin 1 cDNA was manipulated to have multiple cloning sites and a viral specific 3C-protease cutting site at the N-terminal end of the polyprotein. The gene for the N-terminal 169 amino acids of the HIV-1 p24 was cloned into the multiple cloning site of the manipulated Sabin cDNA. A recombinant progeny virus was produced from HeLa cells when it was transfected with the RNA synthesized from the p24-Sabin chimeric cDNA. The recombinant progeny virus expresses substantial amounts of the HIV-1 p24 protein, which was clearly detected in the infected cell lysates and culture supernatants in Western blot experiments with rabbit anti-p24 serum and AIDS patients' sera. Differing from the Mahoney strain, the recombinant Sabin 1 poliovirus maintained the foreign gene stably during the subsequent passages. Replication capacity was about 1 to 1.5 log lower than that of the wild-type Sabin 1. Other physicochemical stability characteristics of the recombinant virus were similar to that of the wild-type Sabin 1. These results suggest that the manipulated Sabin 1 poliovirus can be used as a live viral vaccine vector for the development of mucosal vaccines.

• 한국산학기술학회논문지
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• 제11권5호
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• pp.1905-1911
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• 2010

본 연구는 일개 대학 치위생과 학생들의 후천성 면역결핍증(에이즈)에 대한 지식 및 태도를 알아 보기위해 수행되었다. 265명의 치위생과 학생들을 대상으로 자기 기입식 설문조사를 시행하였다. 전체 문항을 바르게 인지하는 경우를 100점으로 하였을 때 전체 대상자의 에이즈에 대한 지식도는 $76.4({\pm}13.4)$점이었다. 과반수 이상에서 에이즈에 대한 두려움을 나타내었고, 대다수의 응답자가 에이즈 환자를 꺼렸다. 대부분이 만약 에이즈 환자를 치료해야 한다면 굉장히 두려움을 느낄 것이라고 응답하였다. 결론적으로 치위생과 학생들은 에이즈에 대한 지속적인 교육이 필요하다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제43권6호
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• pp.388-394
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• 2017

Objectives: The objective of this study was to investigate the presence of oral lesions in human immunodeficiency virus/acquired immunodeficiency syndrome (HIV/AIDS) patients in a descriptive cross-sectional study, and to establish their presence according to levels of CD4+ cells (including the CD4+/CD8+ cell ratio). Materials and Methods: A total of 75 patients infected with HIV were included. Oral lesions were observed and classified using World Health Organization classification guidelines. Potential correlations between the presence and severity of oral lesions and CD4+ cells, including the CD4+/CD8+ cell ratio, were studied. Results: The most frequent oral lesion detected was oral pseudomembranous candidiasis (80.0%), followed by periodontal disease (40.0%), herpetic lesions (16.0%), hairy leukoplakia (16.0%), gingivitis (20.0%), oral ulceration (12.0%), Kaposi's sarcoma (8.0%), and non-Hodgkin's lymphoma (4.0%). The CD4+ count was <$200cells/mm^3$ in 45 cases (60.0%), between $200-500cells/mm^3$ in 18 cases (24.0%), and >$500cells/mm^3$ in 12 cases (16.0%). The mean CD4+ count was $182.18cells/mm^3$. The mean ratio of CD4+/CD8+ cells was 0.26. All patients showed at least one oral manifestation. Conclusion: There was no correlation between the CD4+/CD8+ cell ratio and the presence of oral lesions. The severity of the lesions was more pronounced when the CD4+ cell count was less than $200cells/mm^3$.

• 대한치과의사협회지
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• 제34권5호통권324호
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• pp.351-361
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• 1996

• 생명과학회지
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• 제9권5호
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• pp.556-563
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• 1999

Presence of antibody to the capsid protein p24 is the main diagnostic criterion, since this reflects reliable antibody response to HIV infection. However, it takes about 6-8 weeks for antibody production after infection and people who are infected but antibodies are not produced yet are classified as seronegative. Therefore, there is a strong need for an improved diagnostic method for better health security. As a first step for developing such an improved diagnostic system, gag protein of human immunodificiency virus type 1 was expressed in E. coli DH5$\alpha$. The gag fragment of HIV-1 (including a portion of p17 and whole p24) was amplified by polymerase chain reaction (PCR) and BamH I/EcoR I sites were created during PCR. The amplified DNA fragment was cleaved with BamH I/EcoR I and was subcloned into the GEX-2T vector which had been digested with BamHI/EcoRI, resulting gene fusion with gst gene of pGEX-2T. The recombinant DNA was transferred into E. coli DH5$\alpha$. The transformed bacteria were grown at 37$^{\circ}C$ for 3h and protein expression was induced with 0.1mM IPTG at $25^{\circ}C$ for 3h. Recombinant gag protein or GST-gag fusion protein was purified with glutathione-sepharose 4B bead and migrated as a single band when analyzed by 10% polyacrylamide gel. These proteins were confirmed by immunoblotting with anti-GST goat sera or Korean AIDS patients sera. The results of this study establish the expression and single step pulification of HIV-1 gag protein which can specifically bind with Korean AIDS patients sera.

• 기본간호학회지
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• 제6권3호
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• pp.544-558
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• 1999

With the increasing incidence of AIDS, it is probably inevitable that nurses working in hospital and community settings will come in contact with patients with AIDS. Nurses, more than any other health care profession, are on the front line of AIDS patient care. The purposes of this descriptive study were to gather information about registered nurses' knowledge and attitude regarding AIDS. and to provide a basis for the development of continuining education programs for the nurse. Data was gathered in 1988 using self-administered questionnaires given to a convinience sample of two hundred seventy registered nurses from two university hospitals, school nurses and postgraduate students of nursing. Data was analyzed with SAS. The results were as follows. Above 90% of the nurse knew well about definition of AIDS and routes of transmission but nurses relatively lacked knowledge about transmission of HIV in breast milk(69.3%). Less than half knew that drug abuser(44.1%) and sexual partner with IV drug abuser(39.6%) are at high risk for contracting AIDS. Above 70% of the nurse showed reluctance to provide care for surgery. delivery of child birth and hemodialysis of patients with AIDS. The results showed that, given a choice. 41.7% of the nurse would refuse to care for AIDS patients and 48.3% claimed that they should have a right refuse to care for AIDS patients. Reluctance of nursing patient with AIDS appeared to be principally associated with general fear of becoming infected with HIV. 41.8% exhibited a sympathetic attitude toward individual AIDS patient. The study findings suggest that it is necessary to examine the correlations between knowledge and attitude and to develop continuing education programs that alleviate the fear of contagion of the nurse.

• 의료법학
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• 제12권2호
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• pp.65-117
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• 2011

This article analyzes the case (2008Da16776) which has the issue how patients have to prove causal relationship when patients claim against pharmaceutical companies alleging that patients were infected with virus due to contaminated blood products. The Supreme court held that: (1) if patients prove that they didn't have symptoms suggesting virus infection before administration of blood products, the virus infection had been confirmed after administration of blood products, and there were significant potential of contamination of the blood products with the virus, the defect in blood products or the negligence of pharmaceutical company in making blood products shall be presumed to cause the infection of the victim. (2) The pharmaceutical companies could reverse the presumption by proving the blood products were not contaminated, but the fact that the victims were treated with the blood products manufactured by other companies or had received blood transfusions is not enough to reverse the presumption. The case is the first decision whether the burden of proof about causal relationship could be reduced in pharmaceutical product liability lawsuit. Hereafter pharmaceutical product liability cases, it would be necessary to reduce the burden of proof about causal relationship in order to make substantive equality between patients and pharmaceutical companies.