• Asian-Australasian Journal of Animal Sciences
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• v.22 no.5
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• pp.659-666
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• 2009

Metabolizable protein (MP) supply and amino acid balance in the intestine were manipulated through selection of highly digestible rumen-undegradable protein (RUP) sources and protected methionine (Met) supplementation. Four ruminallycannulated, multiparous Holstein cows averaging 193${\pm}$13 days in milk were used in a 4${\times}$4 Latin square design to assess N utilization and milk production responses to changes in RUP level, post-ruminal RUP digestibility and protected Met supplementation. Treatments were A) 14.0% crude protein (CP), 8.0% rumen degradable protein (RDP) and 6.0% RUP of low intestinal digestibility (HiRUP-LoDRUP); B) 14.1% CP, 8.1% RDP and 6.0% RUP of high intestinal digestibility (HiRUP-HiDRUP); C) 13.1% CP, 7.9% RDP and 5.2% RUP of high intestinal digestibility (LoRUP-HiDRUP), and D) 13.1% CP, 7.9% RDP and 5.2% RUP of high intestinal digestibility plus rumen escape sources of Met (LoRUP-HiDRUP+Met). Experimental diets were formulated to have similar concentrations of RDP, net energy of lactation ($NE_L$), neutral detergent fiber (NDF), acid detergent fiber (ADF), calcium, phosphorus and ether extract using the NRC model (2001). Results showed that dry matter intake (DMI), production of milk fat and protein were similar among treatments. Milk production was similar for diet HiRUP-LoDRUP, HiRUP-HiDRUP and LoRUP-HiDRUP+Met, and significantly higher than diet LoRUP-HiDRUP. Milk fat and protein percentage were higher for cows receiving HiDRUP treatments, with the greatest increases in the diet LoRUP-HiDRUP+Met. There was no significant change in ruminal pH, $NH_3g-N$ and volatile fatty acid (VFA) concentration among all treatments. Apparent digestibility of dry matter (DM), CP, NDF and ADF and estimated bacterial CP synthesis were similar for all treatments. Nitrogen intakes, blood and milk urea-N concentrations were significantly higher for cows receiving HiRUP diets. Urine volume and total urinary N excretion were significantly lowered by LoRUP diets. Lowering dietary RUP level while supplementing the highly digestible RUP source with rumen escape sources of Met resulted in similar milk production, maximal milk fat and protein concentration and maximum N efficiency, indicating that post-ruminal digestibility of RUP and amino acid balance in the small intestine can be more important than total RUP supplementation.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.15 no.1
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• pp.52-65
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• 2016

The electronic toll collection system(ETCS), is globally used in about 40 countries. In Korea, the Korea Expressway Corporation led the nationwide introduction of the ETC system in 2007 under the brand name of the Hi-pass. Since then, Hi-pass, with its rate of average daily use reaching about 70%, has become an essential facility in Korean expressways. To identify users' perception on Hi-pass (satisfaction, preference, etc.), this study conducted a Modified Importance-Performance Analysis (M-IPA). With this, this study attempted to identify the kind of efforts necessary to enhance current Hi-pass users' convenience and satisfaction. According to the result of M-IPA, the items including "non-stop payment" and "toll discount" are identified as key items that require further improvement. For improvement in the "non-stop payment" item, operations of Hi-pass toll booths appropriate for the demand for Hi-pass use and the selection of Hi-pass booths' locations in consideration of entry and exit lanes need to be improved. In addition, with regard to the "toll discount" item, although toll discount is currently provided to Hi-pass users, thus, PR thereof will have to be strengthened. It is expected that this study will be used as basic data to devise methods of enhancing Hi-pass usage through improvement of Hi-pass users' satisfaction.

• Transactions of the Korean hydrogen and new energy society
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• v.21 no.6
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• pp.479-486
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• 2010

In order to confirm the effect of $HI_x$ solution on Bunsen reaction in Sulfur-Iodine thermochemical hydrogen production process, the reaction was investigated using $HI_x$ solution as a reactant. The phase separation characteristics of reaction with $HI_x$ solution were compared with the reaction using $I_2$ and $H_2O$ as reactants. Firstly, saturation points of $I_2$ in $HI_x$ solution at various temperatures were investigated to determine reaction conditions. With increasing temperature, the amounts of unreacted $I_2$ and $H_2O$ in $HI_x$ solution were increased, while impurities (HI in $H_2SO_4$ phase and $H_2SO_4$ in $HI_x$ phase) in each phase were decreased. The volumes of $H_2SO_4$ phase obtained from Bunsen reaction with $HI_x$ solution was relatively less than those obtained from the reaction with $I_2$ and $H_2O$. The difficulty of phase separation in Bunsen reaction using $HI_x$ solution may be due to the insufficient amount of $H_2O$ existed in $HI_x$ phase after reaction. Therefore, we concluded that the supplement amount of $H_2O$ should be calculated on the basis of the moles of HI and $H_2SO_4$ and added to the reaction system for good phase separation.

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.749-754
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• 2000

PCR primers were designed based on consensus sequences of dTDP-D-glucose 4,6-dehydratase, one of the enzymes involved in the biosynthesis of deoxysugar. The PCR product (360 bp) was obtained from Thermus caldophilus GK24. Colony hybridization was carried out to the cosmid library constructed from T. caldophilus GK24 genomic DNA by the PCR product DNA fragment. We isolated a cosmid clone (pSMTC-1) that was subcloned to call pKCB series plasmid (BamHI fragments), partially sequenced and analyzed. pKCB80 (4.2 kb-BamHI DNA fragment) of them showed ORFs that was orfA, orfB, orfC and orfD. The orfABCD gene cluster is the deosysugar biosynthetic gene ; orfA (glucose-1-phosphate thymidylytransferase), orfB (dTDP-D-glucose 4,6-dehydratase), orfC (dTDP-4-keto-L-rhamnose reductase) and orfD (dTDP-4-keto-6-deoxy-D-glucose 3,5-epimerase). The gene cluster that was related in biosynthesis of dTDP-L-rhamnose was also identified by computer analysis, and we proposed that the biosynthetic pathway of deoxysugar analyzed from DNA sequencing of pKCB80 is from D-glucose-1-phosphate, dTDP-D-glucose, dTDP-4-keto-6-deoxy-D-glucose via dTDP-4-keto-L-rhamnose to dTDP-L-rhamnose.

• International Journal of Stem Cells
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• v.16 no.3
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• pp.281-292
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• 2023

Background and Objectives: Human induced pluripotent stem cell (hiPSC)-derived cardiomyocyte (CM) hold great promise as a cellular source of CM for cardiac function restoration in ischemic heart disease. However, the use of animal-derived xenogeneic substances during the biomanufacturing of hiPSC-CM can induce inadvertent immune responses or chronic inflammation, followed by tumorigenicity. In this study, we aimed to reveal the effects of xenogeneic substances on the functional properties and potential immunogenicity of hiPSC-CM during differentiation, demonstrating the quality and safety of hiPSC-based cell therapy. Methods and Results: We successfully generated hiPSC-CM in the presence and absence of xenogeneic substances (xeno-containing (XC) and xeno-free (XF) conditions, respectively), and compared their characteristics, including the contractile functions and glycan profiles. Compared to XC-hiPSC-CM, XF-hiPSC-CM showed early onset of myocyte contractile beating and maturation, with a high expression of cardiac lineage-specific genes (ACTC1, TNNT2, and RYR2) by using MEA and RT-qPCR. We quantified N-glycolylneuraminic acid (Neu5Gc), a xenogeneic sialic acid, in hiPSC-CM using an indirect enzyme-linked immunosorbent assay and liquid chromatography-multiple reaction monitoring-mass spectrometry. Neu5Gc was incorporated into the glycans of hiPSC-CM during xeno-containing differentiation, whereas it was barely detected in XF-hiPSC-CM. Conclusions: To the best of our knowledge, this is the first study to show that the electrophysiological function and glycan profiles of hiPSC-CM can be affected by the presence of xenogeneic substances during their differentiation and maturation. To ensure quality control and safety in hiPSC-based cell therapy, xenogeneic substances should be excluded from the biomanufacturing process.

• Proceedings of the Korean Geotechical Society Conference
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• 2010.09a
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• pp.664-675
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• 2010

Recently environment-friendly construction method is major trend in both domestic and world constrction fields. In this paper High Functional Performance Agent(Hi-FA) which has various improved engineering characteristics different with conventional Portland cement grouting, such as high viscosity, liquidity, void filling ability, early hardening, and separation resistance, was analyzed by field and laboratory test. Also soil improvement and existing deep foundation protection works were performed and analyzed using Hi-FA.

• Journal of The Korean Astronomical Society
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• v.29 no.spc1
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• pp.95-95
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• 1996

We present the recent results of an HI aperture synthesis mosaic of the Large Magellanic Cloud(LMC), made by combining data from 1344 separate pointing centers using the Australia Telescope Compact Array(ATCA). The resolution of the mosaiced images is 1'.0 (15 pc, using a distance to the LMC of 50 kpc). In contrast with its appearance at other wavelengths, the LMC is remarkably symmetric in HI on the largest scales, with the bulk of the HI residing in a disk of diameter 7.3 kpc. Outer spiral structure is clearly seen, though the features appear due to differential rotation, therefore transient in nature. A good correlation is seen between the supershells previously identified in Halpha (e.g. Meaburn 1980) and HI structures.

• Biomedical Science Letters
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• v.16 no.4
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• pp.331-339
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• 2010

This study aims to evaluate the effect of Photodynamic Therapy (PDT) against methicillin-resistant Staphylococcus aureus with high-level mupirocin resistance (Hi-Mup MRSA). To examine the antimicrobial effect of photogem-mediated PDT against Hi-Mup MRSA, CFU quantifications, bacteria cell viability tests, and disk diffusion antimicrobial susceptibility tests were evaluated. In addition, one of PDT mechanisms was investigated by accumulating photogem ($10\;{\mu}g/ml$) in Hi-Mup MRSA. Photogem-mediated PDT properly inhibited the colony formation of Hi-Mup MRSA. Viable bacteria decreased greatly after a PDT application with photogem $10\;{\mu}g/ml$ at energy density $15\;J/cm^2$. The diameter of the inhibition zone around susceptible disks increased after PDT. In addition, we confirmed the accumulation of photogem in bacteria through fluorescent images. These results demonstrated that excellent photosensitization of Hi-Mup MRSA can be achieved using photogem with 630 nm LED irradiation. Thus, PDT may make survival Hi-Mup MRSA inactive.

• The Bulletin of The Korean Astronomical Society
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• v.35 no.2
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• pp.68.2-68.2
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• 2010

Diffuse interstellar atomic hydrogen (HI) gases are easily shaped into shell-like features by energetic processes such as stellar winds and supernova explosions. The physical characteristics and the Galactic distribution of HI shells and shell-like structures, therefore, are closely related to those of the energy sources. Recently, Inner-Galaxy Arecibo L-band Feed Array (I-GALFA) low-latitude HI survey has been completed. I-GALFA HI survey covers the central part of the first quadrant ($31^{\circ}{\lesssim}1{\lesssim}77^{\circ}$ at b = $0^{\circ}$, ${\mid}b{\mid}\lesssim\;12^{\circ}$) with spatial and velocity resolutions of 3.35′and 0.184 km/s, respectively. The high-angular and high-velocity resolutions enable in-depth investigation of HI shells including the ones of smaller angular sizes. We have found 36 shell candidates with the naked eye. Their angular sizes are distributed from $\sim0.4^{\circ}$ to $\sim12^{\circ}$. About sixteen of them appear to be expanding. We examine associated features at other wavebands and discuss their origin.

• The Bulletin of The Korean Astronomical Society
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• v.37 no.2
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• pp.87.2-87.2
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• 2012

The HI absorption against bright continuum source provides a unique opportunity to measure spin temperature of the neutral hydrogen gas. We find three cases among the sample of the VIVA (VLA Imaging of Virgo galaxies in Atomic gas) study with self absorption of strong central continuum by galactic HI gas. Using the HI flux ratio of emission and absorption, we constrain the spin temperature of the neutral hydrogen gas on their disk. The HI absorption is marginally resolved in these galaxies, which also allows us to probe the kinematics of the absorbing gas. All three galaxies are severely stripped in HI due to the intra cluster medium (ICM). We discuss the influence of environmental processes on the temperature and kinematics of HI in these galaxies.