• Fisheries and Aquatic Sciences
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• v.21 no.3
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• pp.8.1-8.6
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• 2018

Desmarestia japonica (Phaeophyceae, Desmarestiales) was recently established from the Japanese ligulate Desmarestia and is morphologically similar to D. ligulata. This species has been reported only from Japan. However, the taxonomic reports based on additional regional distributions are needed to clarify this taxonomic entity and its species boundaries. Because Desmarestia species have restricted distributions in Korea, we reexamined herbarium specimens of D. ligulata deposited at the National Institute of Biological Resources (South Korea). To improve the amplification efficiency of the polymerase chain reaction and avoid contamination by the DNA of other organisms, we developed taxon-specific molecular markers suitable for DNA barcoding of Desmarestia species. Nuclear ribosomal small subunit RNA (18S rDNA) and mitochondrial cytochrome c oxidase 1 (cox1) regions were selected as target DNA. As a result, both were successfully isolated from herbarium specimens of D. japonica acquired over 10 years. These molecular markers provide useful genetic information for herbarium specimens for which conventional molecular analysis is challenging.

• Korean Journal of Plant Taxonomy
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• v.52 no.3
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• pp.144-155
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• 2022

Daphne kiusiana is an evergreen shrub with dense head-like umbels of white flowers distributed in southern Korea, Japan, China, and Taiwan. Plants in China and Taiwan are recognized as var. atrocaulis by having a dark purple stem, elliptic leaves, and persistent bracts. Recently, plants on Jejudo Island were segregated as a separate species, D. jejudoensis, given their elliptic leaves with an acuminate apex, a long hypanthium and sepals, and a glabrous hypanthium. Morphological variations of three closely related taxa, the D. kiusiana complex, were investigated across the distributional range to clarify the taxonomic delimitation of members of the complex. Twelve characters of the leaf and flower were measured from digitized herbarium specimens using the image analysis program ImageJ and were included in a morphometric analysis, the results of which indicate that the level of variation in the characters is very high. The results of a principal component analysis weakly separated D. jejudoensis from D. kiusiana according to their floral characteristics, such as a longer, glabrous hypanthium, and larger sepals. However, some individuals of D. kiusiana, particularly those from Bigeumdo Island, were included in D. jejudoensis. Recognition of D. kiusiana var. atrocaulis based on the leaf shape was not supported in the analysis, and D. jejudoensis may be recognized as a variety of D. kiusiana. Our morphometric analysis shows that digitized images of herbarium specimens could be useful and an additional method by which to investigate more diverse specimens.

• Korean Journal of Plant Taxonomy
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• v.46 no.1
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• pp.33-54
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• 2016

Many of the historic plant specimens collected from the Korean Peninsula in the early 20th century deposited in herbaria in Korea were destroyed during the Korean War. However, duplicates of the specimens deposited in the Herbarium of the University of Tokyo (TI) remained unharmed. We examined historic plant specimens, identified undetermined specimens, and made a list to provide fundamental information on the early taxonomic history of Korea. One set of duplicate specimens was donated by TI and deposited into the Chonnam National University herbarium (CNU) and into the herbarium of the National Institute of Biological Resources in Korea (KB).

• Journal of Species Research
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• v.2 no.2
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• pp.191-202
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• 2013

The article provides information on type specimens of 150 taxa of vascular plants from Korea, kept in the Herbarium of the Komarov Botanical Institute of the Russian Academy of Sciences. For all specimens, type category is indicated. Lectotypes for the names of Clematis brachyura Maxim., C. spectabilis Palib., Corydalis wilfordii Regel, Poa viridula Palib., Polygonum marretii H. L$\acute{e}$v., P. sagittatum L. var. hallaisanense H. L$\acute{e}$v., P. taquetii H. L$\acute{e}$v. and P. thunbergii Sieb. et Zucc. var. coreana H. L$\acute{e}$v. were designated. Type specimens examined in this article belong to the taxa described by Russian botanists V.L. Komarov, K.J. Maximovicz and I.V. Palibin, French botanist A.A.H. L$\acute{e}$veill$\acute{e}$ (some with co-author E. Vaniot) and others.

• Korean Journal of Plant Taxonomy
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• v.49 no.3
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• pp.203-208
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• 2019

Sparganium coreanum, a barely recognized species in Korea, was rediscovered during a field survey by the authors, who conducted a re-examination of specimens deposited in the Herbarium of the Korea National Arboretum (KH). This species was described initially by H. $L{\acute{e}}veill{\acute{e}}$ from a specimen collected by F. Taquet from Jeju-do (Taquet 2150). Subsequently, however, it was overlooked and unrecognized among South Korean flora. Several populations of S. coreanum were found in the southern part of the Korean Peninsula and on Jeju-do, although it has long been recognized as S. erectum owing to certain vegetative morphological characteristics shared between the two species, such as robust stems, a similar plant height, and globose rhizomes. However, it is distinct from S. erectum by the number of female heads on the lowest inflorescence branch and the size and shape of the fruit. In this study, we provide a detailed description, illustrations, and photographs with a revised taxonomic key for identification of Sparganium species in Korea.

• Journal of Korean Society of Forest Science
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• v.99 no.4
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• pp.447-469
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• 2010

The recent publications of two major works from Ministry of Environment and Korea National Arboretum (Type specimens collected from Korea at the herbarium of the University of Tokyo, five volumes) and National Institute of Biological Resources (Korean type specimens deposited in foreign herbaria and museums, two volumes excluding other animal groups) on Korean vascular plants have resulted in discrepancies needing either correction or clarification. The former published the results of a portion of T. Nakai's collections deposited at TI, while the latter produced the results about collections of G. Koidzumi's, J. Ohwi's, and S. Kitamura's at Kyoto University and of E. H. Wilson's and H. Leveille's at Harvard University. Consequently some omissions, discrepancies, and conflicts resulted, such as the inclusion of conflicting designations of lectotype, isotype, syntype or holotype, erroneous citations of publications, and misinformation. Suh et al.'s publications about type specimens of Korean plants deposited at TI showed 47 conflicting typifications and 20 typographical and other errors. Type information offered by Yim et al. were discussed, and 75 conflicting typification and 30 typographical and other errors from these two volumes were also brought to notice. It is clear that Suh et al. and Yim et al. merely provided type information without consulting original description of each plant species. It is apparent that all these conflicting designation of types, erroneous citations of publications, and other types of misinformation were as a result of the disregard of original citation and the ignorance of basic type concept.

• Journal of Species Research
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• v.7 no.1
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• pp.73-79
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• 2018

The review provides the information about 46 type specimens on 35 taxa of vascular plants from Korea (Fig. 1), kept in the Herbarium of the Komarov Botanical Institute of the Russian Academy of Sciences in addition to 150 taxa published before. Lectotype of Ligustrum patulum Palib is designated here.

• Korean Journal of Plant Taxonomy
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• v.33 no.4
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• pp.455-472
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• 2003

The period from 1946 until his retirement from Department of Forest Resources, Seoul National University was a time of research and professional activity on many fronts for Korean Plant Taxonomy. From 1952 to 1984, he travelled south Korea and collected more than 70,000 specimens. Working out of this project, Dr. Lee developed the SNUA herbarium (Herbarium of The Arboretum of Seoul National University) as the best University herbarium in Korea for the modem floristic work. Dr. Lee's first interest rests on his contributions to our understanding of the twig identification in early 1950. Asian deciduous oak taxa have attracted particular focus to him late 1950 and early 1960. Accordingly Dr. Lee has been one of the specialists on systematic study of Asian oak which is a basis of identification in eastern Asia now. Dr. Lee's contributions concerning rare and endangered plants in Korea provide a tremendous information after a Japanese taxonomist, T. Nakai did in early 1920 to late 1930 in Korea. During his fourteen year career from 1970 to 1984, Dr. Lee spent time to investigate flora of many local and National Park sites. Much of his effort was directed to the voucher specimens for which he worked for the Illustrated Flora of Korea and also served as a field collector. These collections at SNUA enabled us to extend our educational outreach from the local to the national flora research. Although Dr. Lee has been and continues to be the soul of SNUA, collection records about his purpose and aim for each trip are not well documented except date and field studied sites. This lesson enables us to tackle the same issue of balancing quantity and quality with well documented specimens now.

• Korean Journal of Plant Resources
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• v.28 no.4
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• pp.487-510
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• 2015

This study was carried out to inventory the vascular plant flora in Deokjeokdo and its adjacent regions (Mungapdo, Soyado), Ongjin-gun, South Korea, from April to October 2014. Based on the voucher specimens, vascular plants in this area consisted of 108 families, 362 genera, 578 species, 5 subspecies, 66 varieties and 4 forms, totally 653 taxa. In the flora of this area, 5 taxa of Korean endemic plants were found distributed in the sites, including Hepatica insularis Nakai and Asarum glabrata (C.S.Yook & J.G.Kim) B.U.Oh. Korean rare and endangered plants found in this area were 1 taxa of Critical Endangered Species (CR) and 5 taxa of Vulnerable Species (VU). The floristic regional indicator plants found in this area were 67 taxa comprising 3 taxa of grade V, 2 taxa of grade IV, 12 taxa of grade III, 3 taxa of grade II and 47 taxa of grade I. Naturalized plants consisted of 67 taxa, such as Chenopodium album L. and Erigeron annuus (L.) Pers. were recognized widely distributed in the area.

• Journal of Microbiology
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• v.43 no.3
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• pp.228-236
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• 2005

Fifty-six species in 36 genera of macrolichens are reported from the Zhongdian area, northwest Yunnan, China during the lichenological expedition for highland macrolichen survey in June, 2004. More than $60\%$ of these species have not been reported in South Korea. All of the 182 collected specimens are deposited in the Korean Lichen Research Institute (KoLRI) at Sunchon National University in Korea, and some of them are duplicated in the lichen herbarium, Cry to gamic Herbarium, Kunming Institute of Botany, Academia Sinica (KUN-L) in China. This is the first report on the macrolichen flora in the visited areas.