• Title/Summary/Keyword: HCG

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Effects of human chorionic gonadotropin-producing peripheral blood mononuclear cells on the endometrial receptivity and implantation sites of the mouse uterus

  • Delsuz Rezaee;Mojgan Bandehpour;Bahram Kazemi;Sara Hosseini;Zeinab Dehghan;Saiyad Bastaminejad;Mohammad Salehi
    • Clinical and Experimental Reproductive Medicine
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    • v.49 no.4
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    • pp.248-258
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    • 2022
  • Objective: This research investigated the effects of human chorionic gonadotropin (HCG)-producing peripheral blood mononuclear cells (PBMCs) on the implantation rate and embryo attachment in mice. Methods: In this experimental study, a DNA fragment of the HCG gene was cloned into an expression vector, which was transfected into PBMCs. The concentration of the produced HCG was measured using enzyme-linked immunosorbent assay. Embryo attachment was investigated on the co-cultured endometrial cells and PBMCs in vitro. As an in vivo experiment, intrauterine administration of PBMCs was done in plaque-positive female mice. Studied mice were distributed into five groups: control, embryo implantation dysfunction (EID), EID with produced HCG, EID with PBMCs, and EID with HCG-producing PBMCs. Uterine horns were excised to characterize the number of implantation sites and pregnancy rate on day 7.5 post-coitum. During an implantation window, the mRNA expression of genes was evaluated using real-time polymerase chain reaction. Results: DNA fragments were cloned between the BamHI and EcoRI sites in the vector. About 465 pg/mL of HCG was produced in the transfected PBMCs. The attachment rate, pregnancy rate, and the number of implantation sites were substantially higher in the HCG-producing PBMCs group than in the other groups. Significantly elevated expression of the target genes was observed in the EID with HCG-producing PBMCs group. Conclusion: Alterations in gene expression following the intrauterine injection of HCG-producing PBMCs, could be considered a possible cause of increased embryo attachment rate, pregnancy rate, and the number of implantation sites.

Effects of Exogenous Human Chorionic Gonadotrophin(HCG) on the Corpus Luteum and Fetus in Pregnant Rats (융모성성선자극호르몬 투여가 임신랫드의 황체및 태아에 미치는 영향)

  • Kim, Young Hong
    • Current Research on Agriculture and Life Sciences
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    • v.8
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    • pp.119-127
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    • 1990
  • The present study was designed to determine the effect of HCG administration to pregnant rats on the dam and its fetus including the corpora lutea, the body weight of the fetus, the resorption of the fetus and the malformations of the fetus in skeletal development when a single dose of HCG 50 IU or 100IU on day 4.8.12 or 16 of pregnancy. The results were summarized as follows: 1. Administration of HCG had no effect on the corpora lutea. 2. Fetal resorption was observed in animals treated with HCG on days 4.8 or 12, high incidence being treated with HCG on day 4 of pregnancy. 3. Administration of HCG had no effect on the weight of the fetus except decreasing in it treated with HCG on day 4 of pregnancy. 4. Administration of HCG might be able to induce the skeletal variations but was not the skeletal malformations and abnormal ossification of the fetus. The extra 14 ribs was observed in animals treated with HCG on days 8 and 16 of pregnancy, respectively. 5. It is suggested that HCG administration to pregnant rats was able to induce the fetal resorption and might be able to induce skeletal variations in the fetus.

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Artificial Induction of Spawning by Human Chorionic Gonadotropin (HCG) or Carp Pituitary Extract (CPE) in Olive Flounder, Paralichthys olivaceus (태반성 성선 자극 호르몬(HCG) 및 잉어 뇌하수체 호르몬(CPE) 처리에 의한 넙치, Paralichthys olivaceus의 인공 산란 유도)

  • PARK In-Seok;KIM Hyung-Bae;CHOI Hee-Jeng;LEE Young-Don;KANG Hae-Won
    • Journal of Aquaculture
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    • v.7 no.2
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    • pp.89-96
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    • 1994
  • Spawning of matured olive flounder, Paralichthys olivaceus was accelerated by using single injection of human chorionic gonadotropin (HCG 1,000 IU/kg BW) or carp pituitary extract (CPE 10 mg/kg BW). The body weight of HCG or CPE treated group slightly improved with $5.6\pm0.4\%$ at 2 days after injection or $6.4\pm0.5\%$ at 3 days after injection, Number of eggs spawned increased in the fish treated with HCG or CPE. Surface floating rates of eggs spawned increased in the fish treated with HCG or CPE. Hatching rate also increased in experimental groups compared to the control. Especially superior quality of eggs were obtained 2 and 3 days after injection from HCG treated groups and 3 and 4 days after injection from CPE treated groups at $20^{\circ}$, respectively.

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Induction of Maturation and Ovulation with HCG Treatment in the Sevenband Grouper Epinephelus septemfasciatus (HCG 처리에 의한 능성어 Epinephelus septemfasciatus의 성숙과 배란유도)

  • Song, Young-Bo;Baek, Hae-Ja;Kim, Hyung-Bae;Soyano, Kiyoshi;Kim, Se-Jae;Lee, Young-Don
    • Journal of Aquaculture
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    • v.21 no.2
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    • pp.96-101
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    • 2008
  • To induce of maturation and ovulation, ovary with different development stage of oocytes of sevenband grouper Epinephelus septemfasciatus(n=51, TL $69.1{\pm}1.0$ cm, BW $5.8{\pm}0.3$ kg) rearing indoor-tank in mature and spawning season(June to July) were investigated by cannulation. Female with yolk globule stage oocyte($300{\sim}500{\mu}m$) was injected with human chorionic gonadotropin(HCG, 500 IU/kg BW). Oocytes developed at diameter $300{\sim}700{\mu}m$ in 24 hrs after the HCG injection, and the distribution ratio of over $800{\mu}m$ of oocytes diameter in the cannulated eggs were $91.3{\sim}98.8%(95.1{\pm}3.7%)$ in 48 hrs after the HCG injection. Ovulation was induced from 7 out of 8 female after the HCG injection. The total volume of stripped eggs was 2,480 mL, and the volume of buoyant eggs was 1,360 mL. The fertilization and hatching rates of buoyant eggs were $56.2{\sim}94.9%$ and $70.7{\sim}97.9%$, respectively. These results suggested that HCG 500 IU/kg BW effects on maturation and ovulation of female sevenband grouper with yolk globule stage of oocyte.

Reproductive Bioligy of the Sevenband Grouper, Epinephelus septemfasciatus I. The Effect of HCG on Ovulation Induction (능성어, Epinephelus septemfasciatus의 번식생물학적 연구 I. HCG 처리에 의한 배란유도)

  • Kim, Byong-Ho;Kim, Kyong-Min;Lee, Young-Don;Song, Choon Bok;Rho, Sum
    • Journal of Aquaculture
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    • v.10 no.1
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    • pp.55-61
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    • 1997
  • The experiment was conducted to understand the effect of HCG upon ovulation induction and egg maturation in the sevenband grouper, Epinephelus septemfasciatus, as a part of the research for its resources management and seed production. Three different size groups of sevenband groupers showing 300~400g, 1,500~2,000g and 2,500~4,000g, were collected from June to August, 1996. They were treated two or three times with HCG (500~1,000 IU/kg). Gonadal development of the sevenband groupers were examined using the paraffin section method and then stained with haematoxylin and eosin. When smaller groups of sevenband grouper having 300~400g, 1,500~2,00g and 2,500~3,200g in weight were treated two or three time with HCG 9500~1,000 IU/g), their gonads contained oogonia, oocytes with the size of 20~30${\mu}{\textrm}{m}$, and stroma tissues. However, in case of two HCG treatments with a dosage of 1,000 IU/kg on sevenband groupers of 3,400~4,000g, both healthy eggs of $800\mu$m in diameter and regressing eggs were ovulated. A sevenband grouper of 4,000g obvulated 4,252 eggs in total. Of these, healthy and regressing eggs turned out to be 20.8% and 79.2%, respectively. In addition, previtellogenic oocytes of around $50\mu$m, oocytes of 20~$30\mu$m, and ovulation trace were also observed in the gonad.

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A Comparative Study on Clinical Effectiveness of Human Chorionic Gonadotropin and Progesterone on Luteal Support in Controlled Ovarian Hyperstimulatian far IVF (체외수정 시술시 과배란 유도에서 Luteal Phase Support에 Human Chorionic Gonadotropin과 Progesterone의 효용성에 관한 비교 연구)

  • Nah, O-Soon;Lee, Sang-Hoon;Bae, Do-Whan
    • Clinical and Experimental Reproductive Medicine
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    • v.21 no.3
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    • pp.233-240
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    • 1994
  • This study was conducted to compare the endocrine milieu, and pregnancy rates in In Vitro Fertilization and Embryo Transfer(IVF-ET) program employing combined with gonadotropin releasing hormone agonist(GnRH-a) and pergonal(LH 75lU+FSH 75lU) when either human chorionic gonadotropin(HCG) or progesterone were used for luteal phase support. A total number of 40 IVF-ET treatment cycles were prospectively studied. Ovarian hyperstimulation method was modified ultrashort protocol using GnRH-a. All patients started Decapeptyl at menstrual cycle day # 2, and HMG was started at # 3 days. When leading follicle was ${\geqq}$18mm or at least two follicles were ${\geqq}$14mm in diameter, HCG 10000lU intramuscularly was injected. After 36 hours HCG administration, oocytes were retrieved as usual guided by transvaginal ultrasound. Embryo were transfered 36-48 hours later. The patient's cycles were prospectively randomized to receive HCG(20cycles) or Progesterone (20cycles) for luteal support. The progesterone group received 25mg 1M starting from the day of ET. The HCG group received 1500IU 1M. on days 0, +2, +5 after ET. Estadiol($E_2$) and Progesterone($P_4$) were measured on the day of oocyte aspiration, ET day, and every 6 days thereafter. Results were follows as; 1. Estradiol, progesterone and LH levels on the day of HCG trigger, retrieved oocytes and number of transfered embryo were not significantly different in both groups. 2. On the day of aspiration and embryo transfered day, $E_2$, $P_4$ level were significantly higher in progesterone group than HCG group(p<0.01). 3. $E_2$, $P_4$ level on 6 days after ET were significantly higher in progesterone group than HCG group(p<0.01). But, $P_4/E_2$ ratio was not different in both groups. 4. $E_2$, $P_4$ level 12 days after ET were decreased abruptly in both groups and higher hormonal level appeared in HCG group(P<0.01). 5. The total pregnancy rate in the HCG group was 40% (8/20) and in the progesterone group 15%(3/20). 6. Comparing the pregnant and nonpregnant cases progesterone group was not different the hormonal status. In HCG group, pregnant cases appeared in higher $P_4$, $P_4/E_2$ ratio than nonpregnanct cases(P<0.01).

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Effectof of Hormonal Treatment of Induced Maturation and Ovulation in the Spotted Halibut, Verapser variegatus (호르몬 처리에 의한 범가자미, Verasper variegatus 의 성숙과 배란유도)

  • Baek, Hea-Ja;Kim, Yoon;An, Cheul-Min;Cho, Kee-Chae;Myeong, Jeong-In;Lee, Nam-Ju
    • Journal of Aquaculture
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    • v.13 no.1
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    • pp.47-53
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    • 2000
  • Hormone induction in maturation and ovulation was carried out with spotted halibut (Verasper variegatus) which normally does not spawn spontaneously in captivity. Prior to spawning females were treated with human chorionic gonadotropin(HCG 265-678 IU/kg BM) 17 ${\alpha}$-hydroxy 20-${\beta}$-dihydroprogesterone (17 ${\alpha}$ 20${\beta}$ OHP 0.5-1.0${\mu}$g/kg BW) and luteinizing hormone-releasing hormone analogue pellet (LHRHa pellet 63-81 ${\mu}$g/kg BW) In all the trials HCG injection succeeded in inducing ovulation at 265-300 IU/kg BW. HCG at 620 or 678 IU/kg BW was ineffective in inducing a good rate of ovulation. Final maturation and ovulation were not stimulated by treatment of 17 ${\alpha}$ 20${\beta}$OHP and LHRHa pellet. These results suggest that HCG injection (around 300 IU/kg BW) is more effective compared with LHRHa pellet implantation.

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Effect of Gonadotropin Administration on the Timing of Ovulation, Fertilizable Life of Eggs and Cleavage of Embryos in Rabbit (성선자극 호르몬 투여가 토끼의 배란시간, 난자의 수정능력 보유시간과 난할 속도에 미치는 영향)

  • 김병기
    • Journal of Life Science
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    • v.8 no.5
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    • pp.576-581
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    • 1998
  • The present study was carried out to investigate the effect of gonadotropin administration on the timing of ovula-tion, fertlizable life of eggs and cleavage of embryos in rabbit. Mature angora rabbits were primed for superovulation with PMSG 100IU. Eighty hours later, the rabbit were induced to ovulate with HCG 100IU. Ovulation had started at 10hours after HCG injection and finished at about 16hours. Fertilizable life of eggs were lasted for 8hours after ovulation. The most frequent developmental stage observed from the embryos recovered at 24, 48, 72, 96, and 120 hours after HCG injection was 2-ceIL, 16-cell, morula, blastocyst and blastocyst, respectively.

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Effects of Bisphenol A and Nonylphenol on In Vitro Steroid Production in Matured Oocyte of Greenlings, Hexagrammos agrammus (Bisphenol A와 Nonylphenol이 노래미, Hexagrammos agrammus 성숙기 난모세포의 스테로이드 생성과정에 미치는 영향)

  • Hwang, In Joon;Kim, Hyung Bae;Baek, Hea Ja
    • Development and Reproduction
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    • v.12 no.3
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    • pp.275-281
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    • 2008
  • Endocrine disrupting chemiclas (EDCs) such as bisphenol A (BPA) and nonylphenol (NP) have estrogenic activity and can alter reproduction in fish. In the present study, the effects of BPA and NP on in vitro steroid production from oocytes of maturation stage (oocyte diameter$\fallingdotseq$1.88 mm) from the greenling (Hexagrammos agrammus) were evaluated. Oocytes were incubated with different concentrations of BPA and NP (0.1, 1, 10, 100 and 1,000 ng/$m{\ell}$) in the presence or absence of 50 IU human chorionic gonadotropin (HCG) for 48 hours. After incubation, levels of $17{\alpha},\;20{\beta}$-dihydroxy-4-pregnen-3-one ($17{\alpha}20{\beta}OHP$), estradiol-$17{\beta}(E_2)$ and testosterone (T) from incubated media were quantified by radioimmunoassay (RIA). In BPA treatment, 100 ng/$m{\ell}$ of BPA stimulated $E_2$ production regardless HCG supplement. Every concentration of BPA inhibited T production without HCG although 0.1 ng/$m{\ell}$ of BPA stimulated T production with HCG. In NP treatment, 10 ng/$m{\ell}$ of NP stimulated $17{\alpha}20{\beta}OHP$ and T production without HCG. 1 ng/$m{\ell}$ of NP inhibited $E_2$ production. Taken toghther, these results suggest that BPA might have weak estrogen-agonistic effect and NP has estrogenantagonistic effect at final oocyte maturation stage of H. agrammus.

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Coffee consumption behaviors, dietary habits, and dietary nutrient intakes according to coffee intake amount among university students (일부 대학생의 커피섭취량에 따른 커피섭취행동, 식습관 및 식사 영양소 섭취)

  • Kim, Sun-Hyo
    • Journal of Nutrition and Health
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    • v.50 no.3
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    • pp.270-283
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    • 2017
  • Purpose: This study was conducted to examine coffee consumption behaviors, dietary habits, and nutrient intakes by coffee intake amount among university students. Methods: Questionnaires were distributed to 300 university students randomly selected in Gongju. Dietary survey was administered during two weekdays by the food record method. Results: Subjects were divided into three groups: NCG (non-coffee group), LCG (low coffee group, 1~2 cups/d), and HCG (high coffee group, 3 cups/d) by coffee intake amount and subjects' distribution. Coffee intake frequency was significantly greater in the HCG compared to the LCG (p < 0.001). The HCG was more likely to intake dripped coffee with or without milk and/or sugar than the LCG (p < 0.05). More than 80% of coffee drinkers chose their favorite coffee or accompanying snacks regardless of energy content. More than 75% of coffee takers did not eat accompanying snacks instead of meals, and the HCG ate them more frequently than LCG (p < 0.05). Breakfast skipping rate was high while vegetable and fruit intakes were very low in most subjects. Subjects who drank carbonated drinks, sweet beverages, or alcohol were significantly greater in number in the LCG and HCG than in the NCG (p < 0.01). Energy intakes from coffee were $0.88{\pm}5.62kcal/d$ and $7.07{\pm}16.93kcal/d$ for the LCG and HCG. For total subjects, daily mean dietary energy intake was low at less than 72% of estimated energy requirement. Levels of vitamin C and calcium were lower than the estimated average requirements while that of vitamin D was low (24~34% of adequate intake). There was no difference in nutrient intakes by coffee intake amount, except protein, vitamin A, and niacin. Conclusion: Coffee intake amount did not affect dietary nutrient intakes. Dietary habits were poor,and most nutrient intakes were lower than recommend levels. High intakes of coffee seemed to be related with high consumption of sweet beverages and alcohol. Therefore, it is necessary to improve nutritional intakes and encourage proper water intake habits, including coffee intake, for improved nutritional status of subjects.