• Korean Journal of Plant Resources
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• v.19 no.6
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• pp.680-686
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• 2006

This experiment was conducted on wild vegetables; Gondalbi (Cirsium setidens), Deoduck (Codonopsis lanceolata Trautv.), and Jandae (Adenophora triphylla var. Joponica Hara) seed to study whether priming with deep sea water results in enhancement of seed germination and identify the optimum concentration of the priming solution, and duration of priming. Seeds were primed with 5 various concentrations (5%, 10%, 15%, 20% and 30%) of deep sea water (DSW) in 12 hours, 24 hours, and 48 hours at $24^{\circ}C$. Since Jandae had seed dormancy, it was kept for four weeks in refrigerator at $2^{\circ}C$ after priming treatment. In Deoduck, 5 percentage DSW priming significantly improved the early germination percentage, radicle length, and plumule emergence percentage. Among the priming period of treatments, 24 hours priming showed better performance in this treatment whereas, in Jandae, 12 hours priming with 10 percentages DSW significantly improved the germination percentage and germination rate. This treatment had increased the final germination percentage by 54%, 15% and 40% compared with control, plain water and $KNO_3$ priming respectively. But in Gondalbi, priming did not improve the germination of seed. However, among the priming treatments, 12 hours priming with 3% $KNO_3$ and 20% DSW gave better performance. In both the wild vegetables; Deoduck and Jandae, priming in deep sea water had improved the germination percentage and germination rate as compare to plain water, $KNO_3$, and without priming treatment. Hence the best seed priming treatment on Deoduck and Jandae are 24 hours with 5% DSW and 12 hours with 10% DSW respectively.

• Korean Journal of Medicinal Crop Science
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• v.25 no.6
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• pp.411-417
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• 2017

Background: Adenophora triphylla var. japonica (Regel) H. Hara shows vegetative growth with radical leaves during the first year and shows reproductive growth with cauline leaves and bolting during the second year. In addition, the shape of the plant varies within the same species. For this reason, there are limitations to classifying the species by visual examination. However, there is not sufficient genetic information or molecular tools to analyze the genetic diversity of the plant. Methods and Results: Approximately 34.59 Gbp of raw data containing 342,487,502 reads was obtained from next generation sequencing (NGS) and these reads were assembled into 357,211 scaffolds. A total of 84,106 simple sequence repeat (SSR) regions were identified and 14,133 primer sets were designed. From the designed primer sets, 95 were randomly selected and were applied to the genomic DNA which was extracted from five plants and pooled. Thirty-nine primer sets showing more than two bands were finally selected as SSR markers, and were used for the genetic relationship analysis. Conclusions: The 39 novel SSR markers developed in this study could be used for the genetic diversity analysis, variety identification, new variety development and molecular breeding of A. triphylla.

• The Korea Journal of Herbology
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• v.36 no.5
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• pp.125-133
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• 2021

Objectives : Thymus quinquecistatus var. japonica (H.Hara) is a member of the genus Thymus of perennial aromatic herb, and it's designated as a natural monument of South Korea. It has traditionally been known to have protective or therapeutic effects on various human disease including cerebrovascular and neurological disease. Recently it was suggested that essential oil extracted from thyme has anti-fungal and anti-bacterial effect. The aim of this study is to investigate anti-inflammatory effect of Thymus quinquecistatus var. japonica in Raw 264.7 macrophage cell line. Methods : The cytotoxic effects of water and 70% ethanol extracts from Thymus quinquecistatus var. japonica, was tested using MTT assay. Inhibitory effects of the extracts to nitric oxide production and mRNA expression of inflammatory cytokines were examined by RT-PCR. Also, MitoSOX-red assay and JC-1 assay were performed to determine if the extracts can inhibit mitochondrial ROS accumulation and maintain mitochondrial membrane potential. Results : In LPS-induced inflammatory response, the extracts efficiently reduced nitric oxide NO production through inhibiting mRNA expression of iNOS enzyme. In addition, expression of the proinflammatory cytokines, IL-1𝛽 and IL-6, was also down-regulated by the extract treatments. Excessive accumulation of mitochondrial ROS induced by LPS was inhibited in the extract treated cells, which finally protected mitochondrial membrane potential. Conclusions : These results showed that water and 70% ethanol extracts from Thymus quinquecistatus var. japonica have anti-inflammatory effect through down regulation of IL-1𝛽, IL-6, and iNOS, and also have antioxidative effect against mitochondrial ROS accumulation that promote inflammatory response.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.6
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• pp.814-820
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• 1997

This study was conducted to establish standard fertilization rates for the cultivation of Korean valerian(Valeriana fauriei var. dasycarpa Hara) which is useful for medicinal and aromatic resources. Nitrogen, phosphate and potassium were treated with four application levels, 0, 6, 9, 12kg per 10a, and the growth characteristics, root yield and essential oil compositions of valerian were measured. The growth and root yield of valerian were the highest in the rate of 9kg per 10a for nitrogen, phosphate and potassium, respectively. Nitrogen had the most powerful effective followed by potassium and phosphate. The contents and compositions of essential oil also varied with the amount of fertilization. The contents of the essential oil was the highest in the application level of 9kg per 10a for nitrogen, phosphate and potassium, respectively. The essential oil compositions were affected by the fertilization rate.

• Journal of Photoscience
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• v.9 no.2
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• pp.379-381
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• 2002

The major electron transfer cofactors in photosystem II have been studied by pulsed EPR, pulsed electron electron double resonance (PELDOR) and laser excited spin polarized electron spin echo envelope modulation (ESEEM) methods, in non-oriented and oriented photosystem II membranes. Distances between radical pairs were determined trom the observed dipole interaction constants to be 27.3 A for P680-QA, 30 A, etc. with the error within 1 A. Angles between the distance vector and membrane normal was determined by orientation dependence of oriented membranes with the accuracy of 5˚ The results were compared with the recent structural data by X-ray analysis.

• Proceedings of the Korea Association of Crystal Growth Conference
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• 1997.06a
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• pp.131-134
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• 1997

Scintillation crystals for industrial field are used in fundamental physics i.e. nuclear and high energy physics experiments besides the medical imaging, process control and gauging, container inspection, mineral process etc. For the reason of limited marketability, there are not so many studies with emphasis paced on the crystal growth. The scintillation crystal is an important theme in the studies in the fundamental physics and researchers for crystal growth are expected participate it. The present work is partly supported by a Grant-in-Aid from the Japanese Ministry of Education, Science and Culture.

• Structural Engineering and Mechanics
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• v.11 no.5
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• pp.519-534
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• 2001

The effect of the initial imperfections on the nonlinear behaviors and ultimate strength of the thin-walled members subjected to the axial loads, obtained by the finite element stability analysis, are examined. As the initial imperfections, the bucking mode shapes of the members are adopted. The buckling mode shapes of the thin-walled members are obtained by the transfer matrix method. In the finite element stability analysis, isoparametric degenerated shell element is used, and the geometrical and material nonlinearity are considered based on the Green Lagrange strain definition and the Prandtl-Reuss stress-strain relation following the von Mises yield criterion. The U-, box- and I-section members subjected to the axial loads are adopted for numerical examples, and the effects of the initial imperfections on the nonlinear behaviors and ultimate strength of the members are examined.

• Structural Engineering and Mechanics
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• v.16 no.3
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• pp.361-369
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• 2003

In this paper, the structural behavior of R/C cylindrical panel is analyzed by experimental results. To avoid the geometric imperfection, R/C shell specimens are made by use of a stiff steel mold. From experimental results, the load carrying behavior of R/C cylindrical panel is presented under an external lateral pressure. Even if R/C shell does not posses geometric imperfections, the inaccuracy of the reinforcement position strongly affects to the ultimate strength and the failure patterns of such shells. To explain these effects, FEM nonlinear analyses are done under the same conditions as those of experiments. The behavior of R/C cylindrical shells are well simulated under the consideration of both the geometric imperfection and several inaccuracies.

• Korean Journal of Plant Taxonomy
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• v.31 no.4
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• pp.383-387
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• 2001

One unrecorded species of Suaeda is found from the western and southern coast of Korea. The morphological characteristics and habitat of the plant are described. The species is distinguished from the other Korean species in the genus by its thin and broad leaves and the stellate shape of fruits.

• BMB Reports
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• v.48 no.6
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• pp.319-323
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• 2015

Mesenchymal stem cells (MSCs) are multipotent stem cells capable of differentiating into adipocytes, osteoblasts, or chondrocytes. A mutually inhibitory relationship exists between osteogenic and adipogenic lineage commitment and differentiation. Such cell fate decision is regulated by several signaling pathways, including Wnt and bone morphogenetic protein (BMP). Accumulating evidence indicates that microRNAs (miRNAs) act as switches for MSCs to differentiate into either osteogenic or adipogenic lineage. Different miRNAs have been reported to regulate a master transcription factor for osteogenesis, such as Runx2, as well as molecules in the Wnt or BMP signaling pathway, and control the balance between osteoblast and adipocyte differentiation. Here, we discuss recent advancement of the cell fate decision of MSCs by miRNAs and their targets. [BMB Reports 2015; 48(6): 319-323]