• Asian Pacific Journal of Cancer Prevention
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• 제14권6호
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• pp.3825-3830
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• 2013

More studies are needed to clarify treatments and prognosis of early esophageal squamous cell carcinoma (ESCC). This retrospective study was designed to review the outcome of surgical treatment for early ESCC, evaluate the results of a left thoracotomy for selected patients with early ESCC, and identify factors affecting lymph node metastases and survival. The clinicopathological data of 228 patients with early ESCC who underwent transthoracic esophagectomy with lymphadenectomy without preoperative adjuvant treatment were reviewed. The ${\chi}^2$ test or Fisher's exact test were used to detect factors related to lymph node metastasis. Univariate and multivariate analyses were performed to identify prognostic factors. There were 152 males and 76 females with a median age of 55 years. Two hundred and eight patients underwent a left thoracotomy, and the remaining 20 patients with lymph nodes in the upper mediastinum more than 5 mm in short-axis diameter by computed tomography scan underwent a right thoracotomy. No lymph node metastasis was found in the 18 patients with carcinoma in situ, while lymph node metastases were detected in 1.6% (1/62) of patients with mucosal tumours and 18.2% (27/148) of patients with submucosal tumours. Only 7 patients showed upper mediastinal lymph node metastases in the follow-up. The 5- and 10-year overall survival rates were 81.4% and 70.1%, respectively. Only histologic grade (P<0.001) and pT category (P=0.001) significantly correlated with the presence of lymph node metastases. In multivariate analysis, only histologic grade (P=0.026) and pT category (P=0.008) were independent prognostic factors. A left thoracotomy is acceptable for selected patients with early ESCC. Histologic grade and pT category affected the presence of lymph node metastases and were independent prognostic factors for early ESCC.

• Asian-Australasian Journal of Animal Sciences
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• 제33권6호
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• pp.965-972
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• 2020

Objective: Astragalus membranaceus root is a well-known traditional Chinese herbal medicine with many biological active constituents. This study was conducted to examine the effects of Astragalus membranaceus root powder (AMP) on growth performance, serum antioxidant and immune response in finishing lambs. Methods: A total of thirty-six Guangling fat-tailed ram lambs (body weight = 19±2 kg, mean±standard deviation) were randomly assigned to one of six treatments for a 40 d feeding period, with the first 10 d for adaptation. Treatments consisted of the lambs' basal diets with addition of 0, 5, 10, 15, 20, and 30 g/kg of diet of AMP. Results: Response to supplementation level of AMP was quadratic (p≤0.032) for final weight and ADG with the greatest at 10 g/kg of diet, but dry matter intake was not affected (p≥0.227) by treatments. The increase of AMP supplementation resulted in a quadratic response in contents of triglyceride and creatinine (p<0.05), with the lowest values for 10 and 20 g/kg of diet, respectively. A linear and quadratic decrease was observed in activity of alkaline phosphatase in serum of lambs. As the AMP supplementation increased, the activities of total superoxide dismutase and total antioxidant capacity increased linearly (p≤0.018) and hydroxyl radical (OH^-) decreased linearly (p = 0.002). For catalase (CAT) and malondialdehyde (MDA), quadratic (p≤0.001) effects were observed among treatments, with the greatest CAT and lowest MDA values at 10 g/kg AMP. Additionally, supplementing AMP up to a level of 10 or 15 g/kg of diet quadratically increased immunoglobulin and interleukin contents in the serum. Conclusion: The results indicated that AMP can be used as natural feed additive in the ration of lambs to improve ADG, antioxidant status, and immune functions, and the optimal dose was 10 g/kg of diet under the condition of this experiment.

• Journal of Microbiology and Biotechnology
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• 제27권12호
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• pp.2180-2189
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• 2017

Psychrophilic phytases suitable for aquaculture are rare. In this study, a phytase of the histidine acid phosphatase (HAP) family was identified in Morchella importuna, a psychrophilic mushroom. The phytase showed 38% identity with Aspergillus niger PhyB, which was the closest hit. The M. importuna phytase was overexpressed in Pichia pastoris, purified, and characterized. The phytase had an optimum temperature at $25^{\circ}C$, which is the lowest among all the known phytases to our best knowledge. The optimum pH (6.5) is higher than most of the known HAP phytases, which is fit for the weak acidic condition in fish gut. At the optimum pH and temperature, MiPhyA showed the maximum activity level ($2,384.6{\pm}90.4{\mu}mol{\cdot}min^{-1}{\cdot}mg^{-1}$, suggesting that the enzyme possesses a higher activity level over many known phytases at low temperatures. The phytate-degrading efficacy was tested on three common feed materials (soybean meal/rapeseed meal/corn meal) and was compared with the well-known phytases of Escherichia coli and A. niger. When using the same amount of activity units, MiPhyA could yield at least $3{\times}$ more inorganic phosphate than the two reference phytases. When using the same weight of protein, MiPhyA could yield at least $5{\times}$ more inorganic phosphate than the other two. Since it could degrade phytate in feed materials efficiently under low temperature and weak acidic conditions, which are common for aquacultural application, MiPhyA might be a promising candidate as a feed additive enzyme.

• Journal of Ginseng Research
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• 제39권3호
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• pp.199-205
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• 2015

Background: Ginsenoside Rb1 (G-Rb1), the major active constituent of ginseng, improves insulin sensitivity and exerts antidiabetic effects. We tested whether the insulin-sensitizing and antidiabetic effects of G-Rb1 results from a reduction in ectopic fat accumulation, mediated by inhibition of lipolysis in adipocytes. Methods: Obese and diabetic db/db mice were treated with daily doses of 20 mg/kg G-Rb1 for 14 days. Hepatic fat accumulation was evaluated by measuring liver weight and triglyceride content. Levels of blood glucose and serum insulin were used to evaluate insulin sensitivity in db/db mice. Lipolysis in adipocytes was evaluated by measuring plasma-free fatty acids and glycerol release from 3T3-L1 adipocytes treated with G-Rb1. The expression of relevant genes was analyzed by western blotting, quantitative real-time polymerase chain reaction, and enzyme-linked immunosorbent assay kit. Results: G-Rb1 increased insulin sensitivity and alleviated hepatic fat accumulation in obese diabetic db/db mice, and these effects were accompanied by reduced liver weight and hepatic triglyceride content. Furthermore, G-Rb1 lowered the levels of free fatty acids in obese mice, which may contribute to a decline in hepatic lipid accumulation. Corresponding to these results, G-Rb1 significantly suppressed lipolysis in 3T3-L1 adipocytes and upregulated the perilipin expression in both 3T3-L1 adipocytes and mouse epididymal fat pads. Moreover, G-Rb1 increased the level of adiponectin and reduced that of tumor necrosis factor-${\alpha}$ in obese mice, and these effects were confirmed in 3T3-L1 adipocytes. Conclusion: G-Rb1 may improve insulin sensitivity in obese and diabetic db/db mice by reducing hepatic fat accumulation and suppressing adipocyte lipolysis; these effects may be mediated via the upregulation of perilipin expression in adipocytes.

• Applied Microscopy
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• 제45권4호
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• pp.242-248
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• 2015

In this paper, three carbon sources, i.e., solid graphite, gaseous CH4 and liquid ethanol, and one solid silicon source were employed to synthesize SiC/C nanocomposite powders by arc-discharge plasma. The processing conditions such as the component ratios of raw materials, atmospheric gases, etc. were adjusted for controllable synthesis of the nanopowders. It is indicated that both of solid graphite and silicon can be co-evaporated and reacted to form nanophases of cubic ${\beta}$-SiC with ~50 nm in mean size and a little free graphite; the carbon atoms decomposed from gaseous $CH_4$ favor to combine with the evaporated silicon atoms to form the dominant SiC nanophase; liquid carbon source of ethanol can also be used to harvest the main ${\beta}$-SiC and minor 6H-SiC phases in the assembly of nanoparticles. The as-prepared SiC/C nanocomposite powders were further purified by a heat-treatment in air and their photocatalytic performances were then greatly improved.

• 디지털융복합연구
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• 제13권4호
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• pp.303-310
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• 2015

최근 게임 기술은 모의 수술 시뮬레이션이나 사이버 모델하우스 구축 등의 여러 응용 분야에까지 적용되어지고 있다. 이러한 응용 분야에서 꼭 필요하고 중요한 기술 중 하나는 3D 모델을 실시간으로 절단하는 것이다. 실시간 모의 절단 기술은 자동차나 건축물의 실시간 해체 시뮬레이션 구축에 꼭 필요하며, 다양한 융복합 산업 분야에서 응용이 가능하다. 기존의 BSP-Tree를 이용한 절단 기법에서는 무한 평면을 기준으로 3D 모델을 두 부분으로 절단시키기 때문에 일반적인 절단 작업에 유용하게 사용하기 어렵다. 본 논문에서는 이러한 문제점을 해결하기 위해 유한한 영역 내에서 3D 모델을 절단하는 기법을 제안하였다. 구체적으로 절단 경로면을 유한하게 정의할 수 있도록 하였으며, 절단 범위 이내에서만 3D 모델을 분열시키도록 하여, 다양한 산업 분야에서 유용하게 사용할 수 있도록 하였다. 본 연구에서 제안한 부분 절단 기법의 유용성을 보이기 위해 원자로 3D 모델의 해체 작업 과정을 모의 시뮬레이션 하는 과정에 적용해 보았다.

• BMB Reports
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• 제42권10호
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• pp.636-641
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• 2009

The penetrating of monoclonal antibodies (mAbs) into solid tumor may be hampered by their large size. The antibody mimetics, composed of two complementarity-determining regions (CDRs) through a cognate framework region (FR), have been demonstrated to have the capacity to penetrate tumors superior to its parental intact IgG. In this study, we used CDR and FR sequences from the humanized anti-HER2 monoclonal antibody trastuzumab to design four antibody mimetics. Then these antibody mimetics were fused to human IgG Fc to generate mimetics-Fc small antibodies. One of the four mimetics-Fc antibodies binds well to HER2-overexpressing SK-BR3 cells and effectively inhibits the binding of trastuzumab. This mimetics-Fc, denoted as HMTI-Fc, was shown to be effective in mediating antibody-dependent cellular cytotoxicity and exhibit an antiproliferative effect in SK-BR3 cells. To our knowledge, the HMTI-Fc antibody shown here is the smallest fully functional antibody and may have a potential for treatment of cancer.

• 한국식품과학회지
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• 제37권3호
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• pp.412-417
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• 2005

본 연구는 저지방 햄버거 패티 제조 시 첨가되는 우지방을 올리브유, 옥수수유, 대두유 및 해바라기유를 각각 50% 대체 수준으로 첨가한 후 이화학적 특성 및 관능적 특성을 조사하여 새로운 품질의 햄버거 개발에 응용하고자 실시하였다. 대조구와 식물성유 처리구간에 다소 차이는 있었지만 화학적 조성의 유의적인 차이는 나타나지 않았다. 식물성유를 첨가한 처리구들은 대조구에 비하여 약 2.0-3.8%정도의 칼로리 함량이 감소하였으며, 가열감량, 크기 및 두께 감소율도 낮게 나타났다. 한편, 대조구는 식물성유 처리구들보다 경도, 응집성, 껌성에서 비교적 높은 값을 보였다. 관능검사 결과, 전체적인 기호도는 올리브유 처리구가 가장 높게 나타났으며, 나머지 식물성유 처리구들은 대조구보다 낮게 나타났다.

• 한국식품과학회지
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• 제20권6호
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• pp.780-786
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• 1988

본 실험은 Staphylococcus aureus로부터 생성되는 enterotoxin A의 분리를 위하여 각종 분리방법을 조사하였고 그 특성들을 상호 비교하였던 바 다음과 같은 결과를 얻었다. Amberlite 수지는 배지로부터 처음 toxin을 분리 하는 데에는 간편한 수지이나 정제도에 있어서는 약 70%수준으로 다른 방법들에 비해 낮은 편이었다. CM-cellu-lose수지는 0.2M phosphate buffer로 용출했을 때 75% 정도의 정제도를 가진 toxin을 분리할 수 있었으나 stepwise법으로 용출했을 때에는 80% 이상으로 정제도를 높일 수 있었고 Amberlite 수지와 함께 사용했을 때에는 90%정도의 toxin을 얻을 수 있었다. Amberlite나 CM-cellulose수지로 정제한 후 gel filtration을 실시하면 정제도를 더 높일 수 있었다. FPLC는 분리도와 정제도에서 가장 우수하였으며 FPLC의 결과에 따라 Amberlite 수지는 toxin 분획을 중심으로 앞의 분획을 그리고 CM-cellulose 수지는 뒤의 분획을 주로 제거 하는 특징을 가진다. 따라서 위의 column중 하나만 사용한 후 FPLC를 사용하면 95%이상의 toxin을 분리하는 것이 가능하였다.

• 한국잡초학회지
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• 제30권1호
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• pp.25-33
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• 2010

여러 환경요인이 자귀풀의 발아 및 출아에 미치는 영향을 생장상과 온실에서 조사하였다. 성숙된 자귀풀 종자의 발아율은 42%로 낮았으나 저장기간이 길어짐에 따라 발아율이 증가하였다. $40^{\circ}C$ 온도조건에서 7개월간 종자를 보관하였을 때는 90% 이상 발아율이 증가되었으나 $25^{\circ}C$와 $4^{\circ}C$에서는 각각 58.9%와 55.1%로 낮게 발아되었다. 휴면 각성된 자귀풀 종자는 $30^{\circ}C$ 항온에서는 91.1% 발아되었고, $30/25^{\circ}C$ 변온에서는 92.4% 발아되었다. $90^{\circ}C$ 고온에서 40분간 전처리한 경우에는 발아율이 94.5%로 가장 높았다. 삼투포텐셜 조절에 의하여 수분스트레스를 유기하였을 때 -0.3 MPa까지는 자귀풀의 발아와 초기 생장은 완만하게 감소되었으나 그 이하 수준으로 낮아질수록 크게 감소되어 -0.6 MPa에서는 전혀 발아되지 않았다. 자귀풀은 포장용수량이 높거나 담수상태 조건에서, 그리고 수심이 깊을수록 초기 생장량이 높은 것으로 미루어 습윤한 밭 조건이나 담수상태의 논에서 잘 생육하는 것으로 사료된다.