• Title/Summary/Keyword: H.283

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Effects of CLA-vegetable Oils and CLA-lard Additives on Quality Characteristics of Emulsion-type Sausage (유화형 Sausage의 품질특성에 식물성유와 동물성유 CLA 첨가가 미치는 영향)

  • Lee, J. I.;Lee, J. H.;Kwack, S. C.;Ha, Y. J.;Jung, J. D.;Lee, J. W.;Lee, J. R.;Joo, S. T.;Park, G. B.
    • Journal of Animal Science and Technology
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    • v.45 no.2
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    • pp.283-296
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    • 2003
  • Emulsion-type sausages were manufactured to investigate the effects of CLA-vegetable oils and CLA-lard on quality characteristics of emulsion-type sausage. Each treatments replaced pork back fat with CLA-corn oil (CLA-CO), CLA-safflower seed oil (CLA-SSO) and CLA-lard (CLA-LD) were stored during 1, 7, 14, 21 and 28 days at 4$^{\circ}C$. The changes in physico-chemical properties, texture, thiobarbituric acid reactive substances (TBARS), fatty acid composition and CLA content of each treatments were measured during 1, 7, 14, 21 and 28 days at 4$^{\circ}C$. During storage, pH of control showed higher pH value than that of CLA-vegetable and CLA-lard treatments. The pH values of all treatments was significantly low at storage 14 days (p<0.05). The color L* and a*-value of all the treatments was not significantly changed with the storage periods. Color b*-value of CLA-treatments was higher than that of control during the storage period. Fatty acids composition was changed by addition of CLA-vegetable oils and CLA-lard. All kinds of fatty acids composition were decreased with CLA replacements than that of the control. Whereas linolenic acid content extremely increased by replacement of CLA-vegetable. CLA content of CLA-treatments was extremely increased than that of the control. The cohesiveness, springness, gumminess and brittleness of CLA-LD treatment was significantly higher (P<0.05) than that of the other treatments. The TBARS value of all treatments were significantly increased (P<0.05) as the storage period was passed. TBARS of sausage products containing CLA-CO was the lowest. Summing up the a forementioned results, emulsion-type sausage manufacturing with CLA-vegetable oil and CLA-lard was not affected in physico-chemical properties and texture characteristics. Also, it may be assumed that the high quality sausage can be manufactured with the extent of storage period and CLA accumulation.

Protective Effect of Jinmu-tang on $H_2O_2$-induced Cell Death in C6 Glial Cells (진무탕(眞武湯)이 $H_2O_2$로 유도된 C6 Glial 세포사에 미치는 영향)

  • Choi, Jung-Hoon;Shin, Yong-Jeen;Ha, Ye-Jin;Cho, Mun-Young;You, Ju-Yeon;Lee, Soong-In;Shin, Sun-Ho
    • The Journal of Internal Korean Medicine
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    • v.33 no.3
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    • pp.272-283
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    • 2012
  • Objectives : The purpose of this study was to investigate the mechanism of protective effect of Jinmu-tang (JMT, Zhenwu-tang) extract on $H_2O_2$-induced cell death in C6 glial cells. Methods : Cultured C6 glial cells of white mice were pretreated with JMT extract and exposed to $H_2O_2$ for inducing cell death. We measure the cell viability by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay and investigate the cell morphology using a light microscope after crystal violet (CV) staining. Reactive oxygen species (ROS) formation was analyzed using a flow cytometer and a fluorescent microscope after staining with 2'7'-dichlorofluorescein diacetate (DCF-DA). DNA fragmentation was analyzed using a flow cytometer after propidium iodide (PI) staining and nuclei morphology was investigated using a fluorescent microscope after 2-[4-amidinophenyl]-6-indo-lecarbamidine dihydrochloride (DAPI) staining. We analyzed expression of Bax, processing of procaspase-3 and poly (ADP-ribose) polymerase (PARP), and activation of nuclear factor-${\kappa}B$ (NF-${\kappa}B$) by western blot method. Tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) secretion was analyzed using Quantikine kit. Results : We determined the elevated cell viability by JMT extract on $H_2O_2$-induced C6 glial cell death. ROS formation, DNA fragmentation, $I{\kappa}B{\alpha}$ phosphorylation, NF-${\kappa}B$ activation, and secretion of TNF-${\alpha}$ induced by $H_2O_2$ are inhibited by JMT extract pre-treatment. JMT extract inhibits Bax expression, processing of caspase-3 and PARP that are critical biochemical markers of apoptotic cell death. Conclusions : These results suggest that JMT extract has a protective effect on $H_2O_2$-induced C6 glial cell death in various pathways.

Crystal Structure of Bithional Sulfoxide, $C_{12}H_6Cl_4O_3S$ (비치오놀 설폭사이드, C12H6Cl4O3S의 결정구조)

  • Sin, Hyeon So;Song, Hyeon
    • Journal of the Korean Chemical Society
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    • v.38 no.4
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    • pp.283-287
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    • 1994
  • The crystal stucture of bithional surfoxide, $C_{12}H_6Cl_4O_3S$, has been determined from 2295 independent reflections collected on an automated CAD-4 diffractometer with a graphite-monochromated $Mo-K\alpha$ radiation. The crystal belongs to the monoclinic, space group P2$_1$/n, with a unit cell dimensions a = 12.448(4), b = 9.740(1), c = $11.815(2)\AA$, $\beta$ = $100.06^{\circ}$, $\mu$ = 9.02 cm$^{-1}$, Dm = 1.76 g/cm$^3$, Dc = 1.75 g/cm$^3$, F(000) = 744, and Z = 4. The structure was solved by the direct method and refined by the least-squares method. The final R values was 0.037 for 2295 independent reflections. Overall conformation of the molecule is folded with respect to central surfur atom. Comparing with the molecular conformation of bithional, one of phenyl rings was swinged with about $180^{\circ}.$ This conformational change in the molecule results in the existance of intramolecular-hydrogen bond of S-O(3)---H-O(1) type and its steric hindrance between this moiety and the other phenyl ring. The two best planes of the phenyl rings have a maximum deviation of 0.009 $\AA$ for C(1) atom. The dihedral angle between two phenyl rings is $99.22^{\circ}.$ In the crystal structure, the molecules are packed with intermolecular-hydrogen bond of O(3)---H-O(2).

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Production of Bio-ethanol from Red Algae by Acid Hydrolysis and Enzyme Treatment (산 및 효소 가수분해를 이용한 홍조류로부터 바이오 에탄올 생산)

  • Choi, Soo-Jeong;Lee, Sung-Mok;Lee, Jae-Hwa
    • Applied Chemistry for Engineering
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    • v.23 no.3
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    • pp.279-283
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    • 2012
  • Bio-ethanol production research using various material has been problemed for solving problems of environment pollution caused by fossil fuels. Red-algae consists of agar, carrageenan, and porphyran. If it is treated by acid, it is able to change useful bio-mass for bio-ethanol. In this study, we found an optimal condition for bio-ethanol production from acid hydrolysate in red-algae. To produce bio-ethanol, Saccharomyces cerevisiae KCCM1129 inoculated to acid hydrolysate of Gelidium amansii. The optimal condition for Gelidium amansii hydrolysis was found to be 30 min reaction at $H_2SO_4$ concentration of 1.5% and $121^{\circ}C$. At this condition, its produced to 7.04 g/L galactose and 1.94 g/L glucose. And acetic acid concentration of 2.0% in agar produced 0.75 g/L galactose. In contrast, Pachymeniopis elliptica was treated with $H_2SO_4$concentration of 1.5%, it produced 6.38 g/L galactose. And Pachymeniopis elliptica treated with acetic acid concentration of 2% produced 0.368 g/L galactose. The optimal condition of ethanol production was found to be 96 h reaction at $H_2SO_4$concentration of 1.0% and $30^{\circ}C$, which produced 3.77 g/L ethanol.

Analysis of Paint used in Streetcar No. 381 of Registered Culture Property (등록문화재 전차381호의 도료 분석)

  • Kim, Soo Chul;Park, Min Soo;Seo, Jeong Hun
    • Journal of Conservation Science
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    • v.28 no.3
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    • pp.277-283
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    • 2012
  • This study conducted analysis of paint samples from a streetcar No. 381 (Registration Cultural Property No. 467) to identify the characteristics of the modern paint. The samples were analyzed by Microscopic observation, infrared spectroscopy and SEM-EDS. The upper part is consist of 26 layers and the under part is 29 layers. And the layers were painted various thickness from $10{\mu}m$ to $100{\mu}m$, and confirmed that several color had been used. The putty was used for surface treatment before painting. According to the results of infrared spectroscopy, paint specimens were identified as alkyd resin. Some bands such as C-H and C=O stretcing, aromatic, C-H bending, C-O stretching were found in spectra. Inorganic analysis showed that gold color on the surface was used copper-based paint. And type of the putty was lacquer-putty that was consist of kaolin, talc and zinc white.

pH Solubility Properties and Improved Dissolution of Pranlukast as an Poorly Water-soluble Model Drug Prepared by Spray-drying with Plasdone S-630 (플라스돈 S-630과 함께 분무건조된 모델 난용성 약물로서 프란루카스트의 pH 용해도 특성 및 용출률 개선)

  • Cho, Won-Hyung;Lee, Young-Hyun;Song, Byung-Joo;Yoo, Seok-Cheol;Lim, Dong-Kyun;Khang, Gil-Son
    • Polymer(Korea)
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    • v.35 no.4
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    • pp.277-283
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    • 2011
  • Solid dispersion is mainly used for improved dissolution of poorly water-soluble drugs. Solid dispersion of pranlukast was prepared by spray-drying with plasdone S-630. When pH of water was high, pranlukast was highly soluble in the solubility experiment of solid dispersions with varying pH. The particle size of pranlukast particles in solid dispersions was measured to be in nanometers scale based on particle size analysis. Zeta-potential analysis confirmed the negative charge of solid dispersion. SEM was used to observe the surface of solid dispersion, which confirmed spherical morphology, DSC and XRD confirmed the amorphous nature of solid dispersions. The in vitro test was carried out to find improved dissolution rate of pranlukast solid dispersion in simulated juice gastric and a controlled experiment was carried out to compare pranlukast solid dispersions with a conventional drug (Onon$^{(R)}$), These results showed the dissolution properties of pranlukast solid dispersions prepared by spray drying proper for the oral pharmaceutical formulation.

Production of Calves Following Transfer of Sexed Hanwoo Embryos and Hanwoo Embryos Cultured In Vitro (한우 체외 수정란 및 성 감별 수정란 이식에 의한 송아지 생산)

  • Min, Chan-Sik;Song, Sang-Hyun;Song, Gwi-Dong;Chung, Woo-Jae;Rho, Chi-Won;Kang, Yang-Soo;Park, Choong-Saeng;Kong, Il-Keun
    • Journal of Embryo Transfer
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    • v.23 no.1
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    • pp.43-49
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    • 2008
  • This study was carried out to examine the efficiency of biopsy methods, and the pregnancy rate, calving and abortion rates, gestation length and birth weight of Hanwoo calves following transfer of fresh, frozen and sexed Hanwoo embryos produced in vitro. The survivability of biopsied embryos was 80.0 and 90.0% using aspiration and punching methods at 24 h after culturing, respectively. The ratios of male and female embryos were 42.1 and 52.6%, respectively, and the percentage of sex unidentified was 5.3%. Pregnancy rates was not significantly different between hCG and control group (46.4 vs. 38.5%), fresh and frozen embryos (41.3 vs. 35.0%), and sexed and IVP embryos (27.5 vs. 41.2%) (p>0.05). Calving and abortion rates of IVP and sexed embryos were not significantly different in calving (85.0 vs. 87.0%) and in abortion (15.2 vs. 13.3%) (p<0.05). Gestation length of IVP and sexed calves were 281.3 and 288.2 days in female and 283.0 and 282.3 days in male, and the birth weight of IVP and sexed calves were 23.6 and 25.0 kg in female and 24.6 and 23.8 kg in male, respectively. There were no difference in gestation length and birth weight between IVP embryos and sexed embryos (p>0.05). Administration of hCG to recipients did not improve the pregnancy rate following transfer of Hanwoo embryos produced in vitro and sexed embryos. Although the production of calves derived from sexed Hanweoo embryos cultured in vitro can be obtained, the efficiency of sexed calves production need to be improved in biopsy methods and pregnancy rate. Further study should be focused on the improvement of pregnancy rates for commercial application of embryo transfer.

Characteristic evaluation of anaerobic co-digestion using desulfurization sludge and primary sludge (탈황슬러지 및 생슬러지를 이용한 혐기성 병합소화 특성평가)

  • Seulki Koo;Woojin Chung;Soonwoong Chang;Myoungsoo Park
    • Journal of the Korea Organic Resources Recycling Association
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    • v.31 no.4
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    • pp.51-58
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    • 2023
  • In this study, anaerobic co-digestion was carried out using desulfurization sludge and sewage sludge (primary sludge) to evaluate the effects of sulfur compounds in anaerobic digestion. The experiment was carried out in the form of a batch test using 500 mL duran bottle, and the mixing ratio of the feedstock was selected based on the ratio of COD/SO4. As a result of the experiment, it was confirmed that the amount of biogas generated and the yield decreased at the mixing ratio of COD/SO4 20 or less. In particular, below COD/SO4 10, it was lower than seed (283.5 mL) which was set without feedstock to correct biogas generated by itself from seed sludge. Methane yield tended to decrease from a ratio of COD/SO4 20 or less to 0.135 m3/kg VS compared to 0.396 m3/kg VS of COD/SO4 50. In addition, compared to 0.0097 m3/kg VS of hydrogen sulfide yield from COD/SO4 50, the ratio of COD/SO4 20 increased sharply to 0.0223 m3/kg VS, and in particular, the highest result was 0.0855 m3/kg VS in COD/SO4 10. Based on these results, it is judged that the effect of sulfide in anaerobic digestion can have an adverse effect if the COD/SO4 ratio decreases to less than 20.

Development of 3 Dimensional Information system for culture and art (문화예술 3차원 정보시스템 개발)

  • Lee, C.W.;Kim, W.S.;Lee, M.S.;Kim, C.H.;Hong, S.W.;Lee, C.J.
    • Journal of the Korea Computer Graphics Society
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    • v.1 no.2
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    • pp.279-283
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    • 1995
  • 21세기 정보화 시대에 효과적으로 대비하기 위해 세계 여러나라에서 추진하고 있는 정보화 사회를 구축하는데 필수적인 요소로 lnfrastructure인 초고속 정보 통신망을 구축하고 있다. 이에 따라 국내에서도 정부주도로 초고속망을 이용한 대국민 서비스 사업을 추진하고 있고, 이 사업의 일환으로 문화체육부에서도 국립중앙박물관을 중심으로 대국민 서비스용 문화정보망 구축을 추진하고 있다. 이러한 문화정보망 사업의 최종 목표는 종래의 개념을 넘어선 인간에게 가장 현실감을 줄 수 있는 "가상박물관"과 같이 문화재를 글, 그림 이외에 3차원 업체형상을 가진 문화재를 3차원적으로 표현하여 보이지 않는 부분을 볼 수 있도록 문화재를 이동, 회전 및 확대, 축소 표현이 가능한 3차원 형상정보 데이타베이스를 구축하여야 한다. 본 과제에서는 이러한 3차원 형상정보 데이타베이스 구축을 위해, 컴퓨터 그래픽스 기술과 인공지능 기술이 복합적으로 구현된 3차원 입체형상 자동 입력 시스템 및 모델링 시스템과 지원 도구 개발을 목표로 진행되고 있다. 이 시스템이 완성되면 문화재 형상의 영구보존, 문화재의 특성(크기,색,무늬,질감) 보존, 문화재 형상 복원 및 문화재의 시대적 상대평가를 용이하게 하는 효과를 얻을 뿐아니라, 컴퓨터 그래픽스 기술 및 정보처리 기술을 문화재 연구와 전시에 적용하여 문화정보망 사업의 구체적 효과를 가시화 할 수 있다.

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A Study on the Rural Youth Programs of Foreign Countries (외국의 농촌청소년 개발사업의 현황과 시사점)

  • Kim, Jeong-Joo;Oh, Hae-Sub
    • Journal of Agricultural Extension & Community Development
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    • v.8 no.2
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    • pp.273-283
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    • 2001
  • The purposes of this study were to review the rural youth programs of selected foreign countries and to draw some implications to the rural youth programs in Korea. The youth development programs reviewed were 4-H Youth Development Program of U.S.A, Rural Youth Information Service of Australia, Rural Youth Job Strategy of Canada, The Urban-Rural Youth Program of U.S.A, and Expert Consultation on Extension Rural Youth Programmes and Sustainable Development of FAO. After reviewing the rural youth programs of selected foreign countries, the authors suggested the following implications for further development of rural youth programs in Korea; 1. The target group of rural youth programs should include urban youth as well as rural youth, and the programs should be focused on enlarging their awareness of the rural community and agriculture. 2. Rural youth programs should be extended beyond agricultural sectors, such as leadership, career development, leisure activity, and cultural life. 3. We should develop some programs to support academic achievement, career development, employment, cultural needs including some strategies to prevent problematic behaviors of rural youth. 4. Rural youth should be supported more opportunities to join the community life to experience and learn various life skills through active participation such as interpersonal skill, leadership skill, and problem-solving skill.

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