• The Bulletin of The Korean Astronomical Society
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• v.44 no.2
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• pp.51.1-51.1
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• 2019

At the commissioning phase of KVN from 2009 to 2013, single-dish survey and monitoring observations were performed toward about 1000 evolved stars and about 60 relatively strong SiO and H2O maser sources respectively. Based on these single-dish results and VLBI feasibility test observations at K/Q/W/D bands in 2014, KVN Key Science Project (KSP) has started from 2015 and will be completed in 2019 as KSP phase I. Here we present the overview of observational studies on evolved stars using KVN. In KSP phase I, we have focused on nine KSP sources which show a successful astrometrically registered maps of SiO and H2O masers using the source frequency phase referencing method. We aim at investigating the spatial structure and dynamical effect from 43/42/86/129 GHz SiO to 22 GHz H2O maser regions associated with a stellar pulsation and development of asymmetry in circumstellar envelopes. Using the combined network KaVA (KVN+Japanese VLBI network VERA), KaVA Large Program titled on "Expanded Study on Stellar Masers: ESTEMA Phase I" was performed from 2015 to 2016. Based on ESTEMA Phase I, EAVN Large Program titled on "EAVN Synthesis of Stellar Maser Animations: ESTEMA Phase II" was also performed from 2018. The ESTEMA II project aims to publish composite animations of circumstellar H2O and SiO masers, which taken from up to 6 long-period variable stars with a variety of the pulsation periods (333-1000 days). The animations will exhibit the three-dimensional kinematics of the maser gas clumps with complexity caused by stellar pulsation-driven shock waves and anisotropy of clump ejections from the stellar surface. Adding three EAVN telescopes (Tianma 65m, Nanshan 26m and NRO 45m telescopes) with KaVA always secures the high quality of the maser image frames through the monitoring program.

• Journal of Embryo Transfer
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• v.9 no.3
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• pp.243-247
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• 1994

최근 의약적으로 유용한 단백질을 대량 생산키 위한 실현 가능한 방법이 유전자변환 가축의 이용과 관련되어 발전되어 왔다. 이러한 유전자 변환동물은 이종의 단백질을 유즙속으로 분비시키는 생체반응기로서 이용되고 있다. 이러한 전략적 목적을 위해 현재 유전자 변환동물의 생산을 위한 이용에 있어 여러 가지 방법들이 보고되고 있다. 그러나 ES 세포의 사용이 이러한 방법들 사이에서 가장 실질적인 것으로 추정되고 있다. 본 실험에서는 유전자 구축을 위해 사람 황체 호르몬(human luteinizing hormone; hLH)의 전사를 유도하기 위해 각각 2.2 및 0.5 kb의 토끼 $\beta$-casein pronoter 단편을 이용하여 생쥐의 유선에 hLH를 발현시키도록 조절하고 발현이 thynidine kinase(TK) pronoter에 의해 좌우되는 neo 유전자를 selectable marker로서 plasnid속에 삽입하였다. 그 결과 생긴 구축 유전자는 각각 pCas 2.2와 pCas 0.5로 명명하였다. 구축된 유전자로 2$\times$107의 TT-2 ES세포를 170V, 550$\mu$F로 100$\mu$g의 선상 plasmid에 의해 electroporation 시켰다. 감염된 colony들은 250$\mu$g/$m\ell$ G418을 함유하는 ESM 배양액에서 선별 7일 이후에 회수하여 성공적으로 감염된 ES세포는 PCR 및 Southern blot에 의해 확인되었고 그들 중 나머지는 trypsin 처리 후 각각 미세조작과 공배양 기술을 사용하여 ICR 생쥐의 8세포기 수정란 속에 도입하였다. 결국 24시간 동안 37$^{\circ}C$, 5% $CO_2$에서 배양된 배반포를 chimera의 생산을 위해 위임신 유기된 G418 선발처리 이후 400 및 275개의 ES 세포 colony가 생존하였으며, 3개의 ES 세포으 colony 의 genome 속에 임의적으로 plamid가 삽입된 것을 Southern blot에 의해 확인되었다. 총 13 chimera 생쥐가 3 colony로부터 생산되었으나 germ-line chimera는 현재 조사중이다. chimera 생산빈도는 공배양 기술보다 주입방법에서 현저히 높았다.

• The Bulletin of The Korean Astronomical Society
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• v.39 no.2
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• pp.114-114
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• 2014

We present our activities linking to planning of possible forms of large program to study on circumstellar H2O and SiO maser sources with KaVA. A great advantage of KaVA for the stellar maser observations is the combination of the unique capability of the multi-frequency phase referencing technique of KVN and the dual-beam astrometry of VERA with the KaVA's relative dense antenna configuration. We have demonstrated this advantage through the test observations conducted by the KaVA Evolved Stars Sub-working Group since 2012 March. Snapshot KaVA imaging is confirmed to be possible in integration time of 0.5 hour at the 22 GHz band and 1.0 hour at the 43 GHz band in typical cases. This implies that large snapshot imaging surveys towards many H2O and SiO stellar masers are possible within a reasonable machine time (e.g., scans on ~100 maser sources within 200 hours). This possibility enables us to select the maser sources, which are suitable for future long-term (10 years) intensive (biweekly-monthly) monitoring observations, from 1000 potential target candidates selected from dual-frequency band (K/Q-bands) KVN single-dish observations. The output of the survey programs will be used for statistical analysis of the structures of individual stellar maser clumps and the spatio-kinematical structures of circumstellar envelopes with accelerating outflows. The combination of astrometry in milliarcsecond(mas) level and the multi-phase referencing technique yields not only trigonometric parallax distances to the masers but also precise position reference for registration of different maser lines. The accuracy of the map registration affects interpretation of the excitation mechanism of the SiO maser lines and the origin of the variety of the maser actions, which are expected to reflect periodic behaviors of the circumstellar envelope with stellar pulsation. Currently we are checking the technical feasibility of KaVA operations for this combination. After this feasibility test, the long-term monitoring campaign program will run as one of KaVA's legacy projects.

• Applied Biological Chemistry
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• v.36 no.2
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• pp.111-114
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• 1993

The volatile components of cooked glutinous rice were investigated. Among more than 100 volatile components of fresh cooked rice, the predominant one was hexanal followed by pentanal, butanal, octanal, heptanal, noananal, acetone and toluene. These eight components comprised about 85% of the total volatiles, and the hexanal comprised about 86% total amount of the eight volatile components. the amount of the eight volatile components and hexanal in cooked glutinous rice were six and nine times more than that of cooked nonglutinous rice, respectively. The amount of volatile components in cooked glutinous rice stored for 72 hours at $70^{\circ}C$ was same with that of in nonglutinous rice immediately after cooking.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.28 no.2
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• pp.111-117
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• 2006

An area of current research is investigating the app1ication of human mesenchymal stem cells or hMSCs as a cell-based regenerative therapy. In order to achieve effective bone regeneration, appropriate matrices functioning as cell-carriers must be identified and optimized in terms of function, efficacy and biocompatibility. Two methods of approaching optimization of matrices are to facilitate adhesion of the donor hMSCs and furthermore to facilitate recruitment of host progenitor cells to osteoblastic differentiation. Pleiotrophin is an extracellular matrix protein that was first identified in developing rat brains and believed to be associated with developing neuronal pathways. A recent publication by Imai and colleagues demonstrated that transgenic mice with upregulated pleiotrophin expression developed a greater volume of cortical as well as cancellous bone. The proposed mechanism of action of pleiotrophin is demonstrated here. Through either environmental stresses and/or intracellular regulation, there is an increase in pleiotrophin production. The pleiotrophin is released extracellularly into areas requiring bone deposition. A receptor-mediated process recruits host osteoprogenitor cells into these areas. Therefore, the aim of our study was to investigate the osteoconductive properties of pleiotrophin. We wanted to determine if pleiotrophin coating facilitates cellular adhesion and furthermore if this has any effect on hMSCs derived bone formation in an animal model. The results showed a dose dependent response of cellular adhesion in fibronectin samples, and cellular adhesion was facilitated with increasing pleiotrophin concentrations. Histologic findings taken after 5 weeks implantation in SCID mouse showed no presence of bone formation with only a dense fibrous connective tissue. Possible explanations for the results of the osteogenesis assay include inappropriate cell loading.

• Economic and Environmental Geology
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• v.26 no.2
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• pp.129-134
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• 1993

At the Janggun mine, boulangerite usually occurs as needles or irregularly-shaped grains, up to $500{{\mu}m}$ in longer dimensions, closely associated with galena, minerals of a tetrahedrite-freibergite series and bournonite in the peripheries of South A and B orebodies and the zone of manganoan carbonates surrounding them. In some places, especially at the top of South B orebody, it occures as "feather ore" consisting of its fine needles or "hairs" in small drusy cavities together with fine-grained euhedral galena, pyrite, manganoan carbonates, quartz, etc. In reflected light, it is bluish grey in colour exhibiting moderate bireflectance and is strongly anisotropic without any internal reflections. Reflectance in air is $R_{max}=43.2$, $R_{min}=35.7$ percent at wavelength of 580 nm, and VHN: $146-173\;kg/mm^2$ at a 50 g-load. The chemical composition on the average from 23 complete spot analyses by electron microprobe is, Pb 56.1, Sb 25.1, S 18.5, Total 99.6 (all in weight percent); the corresponding chemical formula calculated on the basis of S=11 is; $Pb_{5.16}Sb_{3.93}S_{11.0}$ which fulfils approximately the ideal formula $Pb_5Sb_4S_{11}$. The strongest reflections on the X-ray diffraction pattern are; $3.73\;{\AA}\;(10)$, $3.22\;{\AA}\;(5)$, $3.03\;{\AA}\;(4)$ and $2.82\;{\AA}\;(5)$ and the pattern is in harmonic with space group $C^5_{2h}-P2_1/a$. From the textural evidence of the microscopic observations, the mineral is considered to have been formed at the latest stage of hydrothermal lead-zinc-silver mineralization.

• Korean Journal of Ecology and Environment
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• v.36 no.3 s.104
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• pp.235-241
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• 2003

The effect of ultraviolet (UV) radiation on the characteristics of algae-derived dissolved organic matter (DOM) was examined by comparing the biodegradability and DOM fraction distribution of algal DOM before and after UV exposure. Algal DOM from two axenic cultures of Microcystis aeruginosa and Oscillatoria agardhii were irradiated for 24 h at a UV intensity of 42 W/$m^2$. A complete degradation of algal DOM during the UV exposure did not occur, remaining at constant concentrations of dissolved organic carbon(DOC). After UV exposure, however, microbial degradations were reduced by 17% in M. aeruginosa and 53% in O. agardhii, respectively, and decomposition rates also were two times lower in UV exposed algal DOM. In addition, the chemical compositions of algal DOM altered substantially after UV radiation exposure. The proportions of hydrophilic bases (HiB; protein-like DOM) decreased considerably in both algal DOM sources after UV exposure (16.8% and 20.0% of DOM, respectively), whereas those of hydrophilic acids (HiA; carboxylic acids-like DOM) increased as much as the decrease of the HiB fraction. Capillary ion electrophoresis (CE) analysis showed that several carboxylic acids increased significantly after UV exposure, further confirming an increase in HiA fractions. The results of this study clearly indicate that algal DOM can be changed in its chemical composition as well as biodegradability without complete degradation by UV radiation.