• Title/Summary/Keyword: H-bridge

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Isolation and Characterization of Pseudomonas sp. KM10, a Cadmium- and Mercury-resistant, and Phenol-degrading Bacterium

  • Yoon, Kyung-Pyo
    • Journal of Microbiology and Biotechnology
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    • v.8 no.4
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    • pp.388-398
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    • 1998
  • A bacterium which is resistant to both mercury and cadmium, and also capable of utilizing phenol as a carbon and energy source, was isolated from the Kumho River sediments near Kangchang Bridge, Taegu, Korea. The isolate was labeled Pseudomonas sp. KM10 and characterized. The bacteria grew in 4 mM $CdCl_2$and in $70{\mu}M$ $HgCl_2$. The bacteria efficiently removed over 90% of 1 g/l phenol within 30 h. In the presence of 1.250 g/l phenol, the growth of the microorganism was slightly retarded and the microorganism could not tolerate 1.5 g/l phenol. Curing of plasmid from the bacteria was carried out to generate a plasmidless strain. Subsequent experiments localized the genes for phenol degradation in plasmid and the genes for mercury resistance and cadmium resistance on the chromosome. Dot hybridization and Southern hybridization under low stringent conditions were performed to identify the DNA homology. These results showed significant homologies between the some sequence of the chromosome of Pseudomonas sp. KM10 and merR of Shigella flexneri R 100, and between the some sequence of the chromosome of Pseudomonas sp. KM10 and cadA of Staphylococcus aureus pI258. The mechanism of cadmium resistance was efflux, similar to that of S. aureus pI258 cadA, and the mechanism of mercury resistance was volatilization, similar to that of S. flexneri R100 mer.

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A study on single phase UPS inverter control with PDFF method (PDFF 제어기법을 이용한 단상 UPS 인버터 전압, 전류제어에 관한 연구)

  • Oh B. W.;Lee S. Y.;Lee Y. K.;Jeon Y. S.;Choe G. H.
    • Proceedings of the KIPE Conference
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    • 2004.07b
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    • pp.799-802
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    • 2004
  • There are many methods in controlling inverter's voltage and currents. most of all, PI control method is a general method. PI control has some merits. But, PI control has zero effect. So, steady-state response errors always exist by the zero effect. For removing the steady-state error, This paper presents the modeling, design and analysis of the double loop feedback control scheme. and computing the value of parameters and applying In the single-phase full bridge inverter for comparison and analysis between the PI control and PDFF control. The system model is employed to examine the dynamics of power circuit and select appropriate feedback variables for stable operation of the closed-loop UPS inverter system. It analyzes and proves the output characteristic of inverter system with the PDFF control.

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Voltage Source Equipment for the Grid Fault Testing and Analysis of Total Harmonic Distortion According to PWM Methods

  • Gwon, Jin-Su;Kim, Chun-Sung;Kang, Dae-Wook;Park, Jung-Woo;Kim, Sungshin
    • Journal of Power Electronics
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    • v.14 no.6
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    • pp.1081-1092
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    • 2014
  • Renewable energy is being spotlighted as the electric power generating source for the next generation. Due to an increase in renewable energy systems in the grid system, their impact on the grid has become non-negligible. Thus, many countries in the world, including Europe, present their own grid codes for grid power conversion devices. In order to experiment with these grid codes, grid fault test equipment is required. This paper proposes both equipment and a control method, which are constructed with a 7-level cascaded H-bridge converter, that are capable of generating various grid faults. In addition, the Pulse Width Modulation (PWM) method for multilevel converters is compared and analyzed. The proposed structure, the control method, and the PWM method are verified through simulation and experimental results.

Synthesis and Charaterization of Photoluminescent and Electrochemical Property for Diruthenium Complex with Diimine Bridge (디이민 가교제를 갖는 이핵 루테늄 착체의 광발광 및 전기화학적 특성)

  • Kim, Dong-Hwan;Kim, Hwa-Seon;Kwak, Ji-Hoon;Lee, Jee-Hoon;An, Ho-Guen;Woo, Myung-Woo;Ruimao, Hua;Jung, Min-Chul
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2006.11a
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    • pp.296-297
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    • 2006
  • 유기EL의 발광재료로 쓰일 수 있는 이핵 루테늄착체는 $Ru(bpy)_2Cl_2$와 새롭게 디자인한 가교체 (Bis(-2,2'-dipyridyl ketenylidene)-N,N-1,6-diphenylene diamine)의 반응으로부터 합성하였고, FT-IR, $^1H$-NMR, $^{13}C$-NMR, UV-vis, P.L, C.V를 이용하여 착체의 구조분석, 광학적 특성과 전기화학적인 특성을 측정하였다.

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Dielectric Properties depending on Frequency in ITO/$Alq_3$/Al (ITO/$Alq_3$/Al의 주파수 변화에 따른 유전 특성)

  • Oh, Y.C.;Lee, D.K.;Kim, J.S.;Shin, C.G.;Lee, S.I.;Kim, C.H.;Kim, T.W.
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2006.11a
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    • pp.292-293
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    • 2006
  • We have Investigated dielectric properties depending on bias voltage in organic lightemitting diodes using 8-hydroxyquinoline aluminum($Alq_3$) as an electron transport and emissive material. We analyzed the dielectric properties of organic light emitting diodes using impedance characteristics measurement by the auto-balancing bridge technique and equivalent cirrcuit of ITO/$Alq_3$/Al. Impedance characteristics was measured complex impedance Z and phase ${\theta}$ in the frequency range of 40 [Hz] to $10^8$ [Hz]. We obtained complex electrical conductivity, dielectric constant, and loss tangent ($tan{\delta}$) of the device at room temperature. From these analyses, we are able to interpret a conduction mechanism and dielectric properties contributed by an interfacial and orientational polarization.

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Development of a position sensitive CsI(Tl) crystal array

  • Shi, Guo-Zhu;Chen, Ruo-Fu;Chen, Kun;Shen, Ai-Hua;Zhang, Xiu-Ling;Chen, Jin-Da;Du, Cheng-Ming;Hu, Zheng-Guo;Fan, Guang-Wei
    • Nuclear Engineering and Technology
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    • v.52 no.4
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    • pp.835-840
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    • 2020
  • A position-sensitive CsI(Tl) crystal array coupled with the multi-anode position sensitive photomultiplier tube (PS-PMT), Hamamatsu H8500C, has been developed at the Institute of Modern Physics. An effective, fast, and economical readout circuit based on discretized positioning circuit (DPC) bridge was designed for the 64-channel multi-anode flat panel PSPMT. The horizontal and vertical position resolutions are 0.58 mm and 0.63 mm respectively for the 1.0 × 1.0 × 5.0 ㎣ CsI(Tl) array, and the horizontal and vertical position resolutions are 0.86 mm and 0.80 mm respectively for the 2.0 × 2.0 × 10.0 ㎣ CsI(Tl) array. These results show that the CsI(Tl) crystal array with low cost could be applied in the fields of medical imaging and high-resolution gamma camera.

Effects of a Static Synchronous Series Compensator (SSSC) Based on a Soft Switching 48-Pulse PWM Inverter on the Power Demand from the Grid

  • Ustun, Taha Selim;Mekhilef, Saad
    • Journal of Power Electronics
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    • v.10 no.1
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    • pp.85-90
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    • 2010
  • In this paper the effects of a Static Synchronous Series Compensator, which is constructed with a 48-pulse inverter, on the power demand from the grid are studied. Extensive simulation studies were carried out in the MATLAB simulation environment to observe the compensation achieved by the SSSC and its effects on the line voltage, line current, phase angle and real/reactive power. The designed device is simulated in a power system which is comprised of a three phase power source, a transmission line, line inductance and load. The system parameters such as line voltage, line current, reactive power Q and real power P transmissions are observed both when the SSSC is connected to and disconnected from the power system. The motivation for modeling a SSSC from a multi-pulse inverter is to enhance the voltage waveform of the device and this is observed in the total harmonic distortion (THD) analysis performed at the end of the paper. According to the results, the power flow and phase angle can be controlled successfully by the new device through voltage injection. Finally a THD analysis is performed to see the harmonics content. The effect on the quality of the line voltage and current is acceptable according to international standards.

Motion characteristics along the shape of the activating body of a floating wave energy convertor

  • Kim, Sung-Soo;Lee, Su-Bong;Lee, Soon-Sup;Kang, Dong-Hoon;Lee, Jong-Hyun
    • Journal of Advanced Marine Engineering and Technology
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    • v.40 no.8
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    • pp.704-709
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    • 2016
  • Wave energy generation systems can be divided into oscillating water chamber type, over topping device type and wave activating body type. The wave activating body type converts wave energy to kinetic energy, and the power generation amount increases as the motion of an activating body increases. In this paper, the wave energy convertor consists of a main body, which has an H-shape, and the activating body. These are connected by a bar-type bridge. By the incident wave, when the activating body moves with vertical motion this motion is consequently converted into rotational motion. The twisting moment and angular velocity at a shaft of convertor are calculated according to various conditions of the incident wave and the shape of the activating body. This can be used as a basic idea for determining the design of wave activating body type convertor.

Rationalization of allosteric pathway in Thermus sp. GH5 methylglyoxal synthase

  • Zareian, Shekufeh;Khajeh, Khosro;Pazhang, Mohammad;Ranjbar, Bijan
    • BMB Reports
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    • v.45 no.12
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    • pp.748-753
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    • 2012
  • A sequence of 10 amino acids at the C-terminus region of methylglyoxal synthase from Escherichia coli (EMGS) provides an arginine, which plays a crucial role in forming a salt bridge with a proximal aspartate residue in the neighboring subunit, consequently transferring the allosteric signal between subunits. In order to verify the role of arginine, the gene encoding MGS from a thermophile species, Thermus sp. GH5 (TMGS) lacking this arginine was cloned with an additional 30 bp sequence at the 3'-end and then expressed in form of a fusion TMGS with a 10 residual segment at the C-terminus ($TMGS^+$). The resulting recombinant enzyme showed a significant increase in cooperativity towards phosphate, reflected by a change in the Hill coefficient (nH) from 1.5 to 1.99. Experiments including site directed mutagenesis for Asp-10 in TMGS and $TMGS^+$, two dimentional structural survey, fluorescence and irreversible thermoinactivation were carried out to confirm this pathway.

Effective α-Helix Stabilization via Hexenyl Propionate Cross-Link

  • Yoo, Jiyeon;Kim, Young-Woo
    • Bulletin of the Korean Chemical Society
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    • v.35 no.12
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    • pp.3627-3631
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    • 2014
  • In this study we examined two ester-containing cross-links, hex-2-enyl acetate and hex-2-enyl propionate, as new cross-linking systems for helix stabilization of short peptides. We demonstrated that these hexenyl ester cross-links can be readily installed via a ruthenium-mediated ring-closing metathesis reaction of L-aspartic acid 4-allyl ester or L-glutamic acid 5-allyl ester at position i and (S)-2-(4'-pentenyl)alanine at position i+4 using second generation Hoveyda-Grubbs catalyst at $60^{\circ}C$. Between these two cross-links, we found that the hex-2-enyl propionate significantly stabilizes the ${\alpha}$-helical conformations of short model peptides. The helix-stabilizing effects of the hex-2-enyl propionate tether appear to be as powerful as Verdine's i,i+4 all-hydrocarbon stapling system, which is one of the most widely used and the most potent helix-stabilizing cross-linking systems. Furthermore, the hex-2-enyl propionate bridge is reasonably robust against non-enzymatic hydrolytic cleavage at a physiological pH. While extended studies for probing its chemical scopes and biological applications are needed, we believe that this new helix-stabilizing system could serve as a useful chemical tool for understanding protein folding and designing conformationally-constrained peptide drugs.