• Asian-Australasian Journal of Animal Sciences
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• v.32 no.11
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• pp.1789-1800
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• 2019

Objective: Although alveolar macrophages play a key role in the respiratory immunity of livestock, studies on the mechanism of differentiation and survival of alveolar macrophages are lacking. Therefore, we undertook to investigate changes in the lipid metabolism and survival rate, using 3D4/31 macrophages and Dudleya brittonii which has been used as a traditional asthma treatment. Methods: 3D4/31 macrophages were used as the in vitro porcine alveolar macrophages model. The cells were activated by exposure to phorbol 12-myristate 13-acetate (PMA). Dudleya brittonii extraction was performed with distilled water. For evaluating the cell survival rate, we performed the water-soluble tetrazolium salt cell viability assay and growth curve analysis. To confirm cell death, cell cycle and intracellular reactive oxygen species (ROS) levels were measured using flow cytometric analysis by applying fluorescence dye dichlorofluorescein diacetate and propidium iodide. Furthermore, we also evaluated cellular lipid accumulation with oil red O staining, and fatty acid synthesis related genes expression levels using quantitative polymerase chain reaction (qPCR) with SYBR green dye. Glycolysis, fatty acid oxidation, and tricarboxylic acid (TCA) cycle related gene expression levels were measured using qPCR after exposure to Dudleya brittonii extract (DB) for 12 h. Results: The ROS production and cell death were induced by PMA treatment, and exposure to DB reduced the PMA induced downregulation of cell survival. The PMA and DB treatments upregulated the lipid accumulation, with corresponding increase in the acetyl-CoA carboxylase alpha, fatty acid synthase mRNA expressions. DB-PMA co-treatment reduced the glycolysis genes expression, but increased the expressions of fatty acid oxidation and TCA cycle genes. Conclusion: This study provides new insights and directions for further research relating to the immunity of porcine respiratory system, by employing a model based on alveolar macrophages and natural materials.

• Journal of Korean Society of Forest Science
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• v.89 no.5
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• pp.564-575
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• 2000

This study was carried out to reveal characteristics of long-term runoff by using HYCYMODEL in a small forested watershed. From May to September in 1998 and in 1999, the fitness of HYCYMODEL and runoff characteristics were estimated by HYCYMODEL using rainfall and discharge at the experimental watershed. The function of stage and discharge in the experimental watershed was determined as following equation $Q=11.148H^{2.5867}$($R^2=0.9956$). From May to September in 1998 and in 1999, the runoff rates were 57.7% in 1998 and 87.1% in 1999 at the experimental watershed. The discharge was assumed to be increased because of rainfall intensity difference and thinning. By applicability test, the HYCYMODEL showed good estimation of runoff by optimized fifteen parameters. Comparing runoff characteristics before and after thinning by calculating through HYCYMODEL, direct runoff and base runoff increased 4%, 7%, respectively as evapotranspiration decreased 11%. Parameters $D_{50}$ and $K_h$, which were related to the direct run, and a parameter $K_u$, which was related to the baseflow, were assumed to indicate that forest was changed by the effect of thinning and weathering process of bed rock.

• Proceedings of the KSR Conference
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• 2005.05a
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• pp.299-307
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• 2005

The 180 km/h Korean Tilting Train(TTX) which is now developing as a part of the Korean National R&D project, was elaborately designed. As the tilting trains run curve track with the $30\%$ higher speed than normal trains, the higher centrifugal and dynamic force are expected. Furthermore the complex tilting system increase the probability of failure. Therefore it is very important for tilting train to ensure safety against derailment under the various kind of failed condition in the middle of running as well as normal operating condition. The TTX train have the relatively high roll stiffness to improve the lateral ride comfort and to limit the roll displacement on the curve. But the higher roll stiffness increase the risk of derailment on the twisted track. This paper describes the study to review the safety against derailment caused by the wheel unloading on the severely twisted track. The worst combination of maximum cant change with maximum twist defect was established by numerical simulation. And also it was assumed that the air bag deflated and still the train run its speed limit. Those kind of assumption might be the worst case from the view point of wheel unloading derailment on the twisted track. The dynamic simulation was done by means of VAMPIRE S/W and non-linear transient analysis. We found that derailment quotients Q/P was only slightly influenced by track twist but the wheel unloading was greatly influenced. And we ascertained that the higher roll stiffness the higher wheel unloading. In case of air bag deflated situation, the wheel unloading reached up to $100\%$ which means the wheel lift or jumped. Therefore it was concluded that the design need to be improved to ensure the safety against derailment on the maximum twisted track in case of air bag deflated and tilting train's speed limit.

• 유체기계공업학회:학술대회논문집
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• 2003.12a
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• pp.161-166
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• 2003

It is the common features of the integral reactors that the main components of the RCS are installed within the reactor vessel, and so there are no any flow pipes connecting the coolant pumps or steam generators. Due to no any flow pipes, it is impossible to measure the differential pressure at the RCS of the integral reactors, and it also makes impossible measure the flow-rate of the reactor coolant. As a alternative method, the method by the measurement of coolant pump power has been introduced in this study. Up to now, we did not found out a precedent which the coolant pump power is used for the real-time flow-rate calculation at normal operation of the commercial nuclear power plants. The objective of the study is to embody the real-time flow-rate calculation method by the measurement of coolant pump power in an integral reactor. As a result of the study, we could theoretically reason that the capacity-head curve and capacity-shaft power curve around the rated capacity with the high specific-speeded axial flow pumps have each diagonally steep incline but show the similar shape. Also, we could confirm the above theoretical reasoning from the measured result of the pump motor inputs, So, it has been concluded that it is possible to calculate the real-time flow-rate by the measurement of pump motor inputs. In addition, the compensation for a above new method can be made by HBM being now used in the commercial nuclear power plants.

• Earthquakes and Structures
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• v.15 no.3
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• pp.319-334
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• 2018

In this paper, an analytical study was carried out to propose an optimum base-isolated system for the design of steel structures equipped with lead rubber bearings (LRB). For this, 5 and 10-storey steel moment resisting frames (MRFs) were designed as Special Moment Frame (SMF). These two-dimensional and three-bay frames equipped with a set of isolation systems within a predefined range that minimizes the response of the base-isolated frames subjected to a series of earthquakes. In the design of LRB, two main parameters, namely, isolation period (T) and the ratio of strength to weight (Q/W) supported by isolators were considered as 2.25, 2.5, 2.75 and 3 s, 0.05, 0.10 and 0.15, respectively. The Force-deformation behavior of the isolators was modelled by the bi-linear behavior which could reflect the nonlinear characteristics of the lead-plug bearings. The base-isolated frames were modelled using a finite element program and those performances were evaluated in the light of the nonlinear time history analyses by six natural accelerograms compatible with seismic hazard levels of 2% probability of exceedance in 50 years. The performance of the isolated frames was assessed in terms of roof displacement, relative displacement, interstorey drift, absolute acceleration, base shear and hysteretic curve.

• The KSFM Journal of Fluid Machinery
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• v.14 no.6
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• pp.18-25
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• 2011

A boundary-layer pump with a single disk has been experimented to obtain its characteristic curve by changing the impeller of a centrifugal pump to a single disk. The primary objective to use of these types of pumps is to avoid hurting water life during transportation unnecessarily. The change of impeller should degrade the performance of pump, so we used the method to increase the roughness on the disk with sandpaper and mesh. The enhancement of shear force from the rotation of disk to the internal flow brought an augmentation of momentum transport, and the characteristics were far improved from the original single-disk pump without decreasing the survival rate of water life in the case of Pseudobagrus fulvidraco (bullhead fish). However, in the case of Artemia cyst (zooplankton), the survival rate was very degraded due to the micro scale smaller than turbulent eddy size. The result of this study could be used for the design of transportation and bio-filtering of water lying on a specific bandwidth of its scale of size.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2013.10a
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• pp.775-780
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• 2013

In recent years, a notebook like an ultrabook gets thinner. Its thickness causes problems in cooling fan performance, system installation condition, and so on. In this study, we installed a small turbofan in notebook system with very narrow gap in order to generate similar condition to a real product. Experiments were performed to measure the fan's performance and the flow and noise characteristics, its results were compared with computational ones. Prediction of P-Q curve using CFD showed under about 5% error in high flow rate and its trend was agreed with experimental one over the flow field. Experimental data to measure the noise at a distance of 100 mm from a rotation axis direction of an impeller corresponded well with computational ones of broadband and BPF noise. The noise experiments to measure at a distance of 100 mm from a rotation axis direction of an impeller corresponded well with computational ones of broadband and BPF noise. Especially, tip part of impeller blade and part of exit and bottom near in an analysis by a commercial program(FlowNoise).

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.5
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• pp.725-731
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• 2002

This experiment was performed to understand the changes in plasma leptin in association with plasma IGF-1, body weight and ADG from early growing to late finishing stages of Holstein steers. Blood collection was performed by arterial vein puncture at selected monthly ages of 1 (54 kg), 2.6 (103 kg), 7.2 (205 kg), 13.5 (314 kg), 16.9 (414 kg), 22.2 (550 kg), 24.9 (626 kg) and 27.4 months (695 kg). The blood was analyzed for leptin using the multi-species leptin RIA with recombinant bovine leptin (rbleptin) as standard, plasma IGF-1 was also measured using RIA. Against the standard rbleptin, the multi-species Leptin RIA system's sensitivity, cross reactivity, slope and recovery of 41.0 ng/ml rbleptin in plasma were 4.9 ng/ml, 11.22%, -1.396 and 97.8%, respectively. Plasma leptin measured were more than 5.0 ng/ml, which enable multi-species RIA system to investigate plasma leptin in normal growing steers. Body weight resulted to a highly significant second-degree polynomial relationship with plasma leptin (q=0.54, p<0.0001) and plasma IGF-1 (q=0.44, p<0.0001) from 1 to 27.4 monthly ages. However, the second-degree polynomial curve of plasma leptin and IGF-1 differs showing a concave and convex curvilinear relationship, respectively. ADG was not significantly associated to plasma leptin (r=0.06, p>0.05) and plasma IGF=1 (r=0.06, p>0.05) from 1 to 27.4 monthly ages. Low coefficient, but significant associated increase of plasma leptin and IGF-1 (r=0.12, p<0.008) from 1 to 27.4 months was observed. The uncoordinated increases of plasma IGF-1 at growing and plasma leptin at fattening period, may indicate (1) indirect involvement of endogenous IGF-1 on leptin secretion, and (2) IGF-1 level may signify lean and bone accretion while plasma leptin may mirror body fatness across the monthly ages of Holstein steers.

• Journal of the Korean Society of Food Science and Nutrition
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• v.46 no.2
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• pp.210-219
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• 2017

This study aimed to establish an optimal extraction process and high-performance liquid chromatography (HPLC)-photodiode array (PDA) analytical method for determination of marker compounds, dihydrokaempferol (DHK) and 3-O-methylquercetin (3-MeQ), as a part of materials standardization for the development of health functional foods from stems of Opuntia ficus-indica var. saboten (OFS). The quantitative determination method of marker compounds was optimized by HPLC analysis, and the correlation coefficient for the calibration curve showed very good linearity. The HPLC-PDA method was applied successfully to quantification of marker compounds in OFS after validation of the method in terms of linearity, accuracy, and precision. Ethanolic extracts from stems of O. ficus-indica var. saboten (OFSEs) were evaluated by reflux extraction at 70 and $80^{\circ}C$ with 50, 70, and 80% ethanol for 3, 4, 5, and 6 h. Among OFSEs, OFS70E at $80^{\circ}C$ showed the highest contents of DHK and 3-MeQ of $26.42{\pm}0.65$ and $3.88{\pm}0.29mg/OFS100g$, respectively. Furthermore, OFSEs were determined for their antioxidant activities by measuring 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and lipid peroxidation (LPO) inhibitory activities in rat liver homogenate. OFS70E at $70^{\circ}C$ showed the most potent antioxidant activities with $IC_{50}$ values of $1.19{\pm}0.11$ and $0.89{\pm}0.09mg/mL$ in the DPPH radical scavenging and LPO inhibitory assays, respectively. To identify active components of OFS, various chromatographic separation of OFS70E led to isolation of 11 flavonoids: dihydrokaempferol, dihydroquercetin, 3-O-methylquercetin, quercetin, isorhamnetin 3-O-glucoside, isorhamnetin 3-O-galactoside, narcissin, kaempferol 7-O-glucoside, quercetin 3-O-galactoside, isorhamnetin, and kaempferol 3-O-rutinoside. The results suggest that standardization of DHK in OFSEs using HPLC-PDA analysis would be an acceptable method for the development of health functional foods.

• Analytical Science and Technology
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• v.17 no.1
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• pp.37-44
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• 2004

An alcohol oxidase from Hansenula polymorpha was strongly induced when cells were grown with 0.5% methanol supplementation as the carbon source. The induced Hansenula polymorpha alcohol oxidase was purified to electrophoretic homogeneity by using DEAE-Sephacel and Mono Q column chromatographys. The enzyme oxidized mainly primary aliphatic alcohols and exhibited high substrate specificity towards ethanol and methanol. The activity of the enzyme optimally proceeded at pH 8.5 and $50^{\circ}C$. The midpoint of the temperature-stability curve for the enzyme was approximately $52^{\circ}C$ and the enzyme was not completely inactivated even at $65^{\circ}C$ temperature. The enzyme showed resistance toward detergents and highly stable over 7 weeks of storage condition. This Hansenula polymorpha alcohol oxidase may be useful for the enzymatic determination of alcohol and for the industrial production of alcohols and aldehydes.