• Title/Summary/Keyword: H adsorption

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Preparation and Atomic Force Microscopy (AFM) Characterization of DNA Scaffolds as a Template for Protein Immobilization

  • Kim, Hyeran;Lee, Hyun Uk;Lee, Jouhahn
    • Proceedings of the Korean Vacuum Society Conference
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    • 2014.02a
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    • pp.411.2-411.2
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    • 2014
  • The design of DNA nanostructures is of fundamental importance, the intrinsic value of DNA as a building-block material lies in its ability to organize other bio-molecules with nanometer-scale spacing. Here, we report the fabrication of DNA scaffolds with nano-pores (<10 nm size) that formed easily without the use of additives (i.e., avidin, biotin, polyamine, or inorganic materials) into large-scale structures by assembling DNA molecules at near room temperature ($30^{\circ}C$) and low pH (~5.5). Protein immobilization results also confirmed that a fibronectin (FN) proteins/large scale DNA scaffolds/aminopropylytriethoxysilane (APS)/SiO2/Si substrate with high sensitivity formed in a well-defined manner. The DNA scaffolds can be applied for use with DNA-based biochips, biophysics, and cell biology.

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Characterizations of Membrane for Water Treatment: Surface Charge Analysis by Electrophoresis and Acidity Measurements

  • Yongki Shim;Lee, Sangyoup;Moon, Seung-Hyeon;Jaeweon Cho
    • Korean Membrane Journal
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    • v.2 no.1
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    • pp.56-59
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    • 2000
  • The surface charge properties of a polymeric NF and a ceramic UF membranes were characterized in terms of zeta potential and acidity. Both the negative zeta potential and acidity values increased as pH increases due to ionizable acidic functional groups. Increased ionic strength reduced the acidity of the negatively-charged membrane surface as anticipated. Through these results, it can be envisioned are used to reject solutes with ionizable functional groups. Fouling of the negatively-charged membrane with natural organic matter (NOM) having a negative charge density was also investigated with respect to the surface charge. The surface charge of the NF membrane increased negatively when greater NOM adsorption onto the membrane surface occured.

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중성자 산란을 이용한 생체물질의 구조 연구 : 단백질의 생체유사막의 흡착

  • Sin, Gwan-U;Rafailovich, M.H.;Sokolov, J.;Pernodet, N.;Satija, S.K.
    • 한국생물공학회:학술대회논문집
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    • 2002.04a
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    • pp.30-33
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    • 2002
  • We have shown that it is possible to form a fibrilar network of fibronectin on a polyelectrolyte polymer film whose dimensions are similar to those reported on the extra cellular matrix. The fibronectin network was observed to form only when the charge density of the polymer was in excess of the natural charge density of the cell wall. Furthermore, the self-organized fibronectin layer was much thicker than the polymer film, indicating that long ranged interaction may playa key role in the assembly process. It is therefore important to understand the structure of the polymer layer/protein interface. Here we report on a neutron reflectivity study where we explore the structure of the polyelectrolyte layer, in this case sulfonated polystyrene (PSSx,), with varying degree of sulfonation (x<30%), as a function of sulfur content and counter ion concentration. These results are then correlated with systemic study of the adsorption and the multilayer formation of fibronectin as a function of incubation time for various sulfonation levels of $PSSx.^1$

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Two-dimensional(2D) self-assembly of fine gold nanospheres using laser patterning (레이저 패턴을 이용한 금 나노입자의 2 차원적 자기조립)

  • Huh K.S.;Cho S.H.;Kim J.G.;Chang W.S.
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2006.05a
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    • pp.475-476
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    • 2006
  • Self-assembled monolayers (SAMs) formed by the adsorption of alkanethiols, HS(CH2)nX, where X is an organic functional group, onto gold surfaces have attracted widespread interest as templates for the fabrication of molecular and biomolecular microstructures. Previously photopatterning has been thought of as being restricted to the micron scale, because of the well-known diffraction limit. So, we have explored a novel approach to nanofabrication by utilizing a femtosecond laser.

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Desorption Characteristics of $H^{14}CO_3$ ion from Spent Ion Exchanged Resin by Solution Stripping Technology

  • Park Geun-IL;Kim In-Tae;Kim Kwang-Wook;Lee Jung-Won;Won Jang-Sik;Yang Ho-Yeon
    • Proceedings of the Korean Radioactive Waste Society Conference
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    • 2005.11b
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    • pp.214-221
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    • 2005
  • Spent ion-exchanged resin generated from various purification systems in CANDU reactor is causing concern due to a limited storage capacity and safe disposal. As a suggestion for a proper treatment technology for the spent ion-exchanged resin containing a high activity of C­14 radionuclide which would be classified as Class A and C wastes, a fundamental study for the development of C-14 removal technology from a spent resin was performed. The adsorption characteristics of the inactive $HCO_3^-$ ion and other ions in a stripping solution on IRN-150 mixed resin was evaluated and the removal technology of the $HCO_3^-$ ion adsorbed on IRN-150 by an alkaline stripping method was proposed.

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Principle Component Analysis on Electrokinetic Measurements for Amphoteric Fibers/Acid Dye System (앰포테릭섬유/산성염료계의 계면동전압 측정치에 대한 PCA)

  • Park, Byeong-Gi
    • Journal of Korean Society for Quality Management
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    • v.13 no.1
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    • pp.26-30
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    • 1985
  • In the light of the properties of colloids, in the surface of disperse phase and dispersion, there exist specific characters such as adsorption or electric double layer, which seems to play important roles in determining the physiochemical properties in the dyeing system. Nylon, wool and silk, the typical amphoteric fibers were dyed with Acid dye and various combinations were prepared by combining pH, temperature and dye concentration, in order to generate flowing electric potential which were measured by microviolt meter and specific conductivity meter. The results were transformed to Zeta potential by Helmholtz-Smoluchowski formular and to surface electric charge density by Suzawa formular, surface dye amount, and effective surface area of fibers, and these data were statistically analysed by principle component analysis.

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Monoamine Oxidase Inhibitors from Basidiomycete 8082

  • Lee, In-Kyoung;Yun, Bong-Sik;Kim, Yung-Ho;Lee, Myung-Koo;Yoo, Ick-Dong
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 1998.11a
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    • pp.132-132
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    • 1998
  • It has been known that MAO (monoamine oxidase) catalyses the oxidation of endogenous neurotransmitter amines. From its key role in the regulation of some physiological amines, it has been the target of inhibitors used as antidepressive agents. In our continuing search for MAO inhibitors from Basidiomycete. sp., strain 8082 was selected. Two metabolites (8082-1 and 8082-2) were isolated by adsorption chromatography and HPLC from the culture broth of strain 8082. The structure of 8082-1 and 8082-2 were elucidated by $^1$H-, $\^$13/C-NMR and HMBC spectral data, and these products were identified as 5-methylmellein and nectriapyrone, respectively, which have significant inhibitory effect against mouse brain MAO.

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Formation and Treatment Methods of N-Nitrosodimethylamine (NDMA) in Water and Wastewater (상하수에서 N-Nitrosodimethylamine (NDMA) 발생 및 처리법 비교 분석)

  • Kim, Jongo;Lee, Woo-Bum;Park, Soo-Ho
    • Journal of Korean Society on Water Environment
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    • v.30 no.1
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    • pp.95-101
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    • 2014
  • Overall studies for the N-nitrosodimethylamine (NDMA) formation and treatment methods were conducted. In this study, occurrence in several countries and emerging treatment technologies of NDMA were generally reviewed. The NDMA formation was dependent on pH, contact time, and molar ratio (monochloramine/dimethylamine). The formation was rapidly increased when the molar ratio was greater than 1. It was likely that monochloramine could be related to stimulate NDMA formation. NDMA concentrations in water supply and wastewater plants after disinfection were approximately 10 ng/L and 100 ng/L, respectively. UV oxidation and adsorption processes are regarded as effective technologies for the NDMA removal. It is suggested that the UV oxidation with proper lamps is applied in water supply system.

Isolation and Characterization of 4-hydroxycinnamic Acid with Antimicrobial Activity from Aralia eiata (두릅에서 항미생물활성을 갖는 4-hydroxycinnamic acid의 분리 및 동정)

  • Ma, Seung-Jin;Kuk, Ju-Hee;Ko, Byoung-Seob;Park, Keun-Hyung
    • Applied Biological Chemistry
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    • v.39 no.4
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    • pp.265-267
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    • 1996
  • The methanol extracts of AraIia elata showed antimicrobial activity against bacteria. The antimicrobial active principle was successively purified with solvent fractionation, silica gel adsorption column chromatography, Sephadex LH-20 column chromatography, silica gel partition column chromatography and HPLC. The active substance was isolated by HPLC on $C_{18}$ column with an acetic acid-MeOH system. The active substance was identified as trans-4-hydroxycinnamic acid by MS, $^1H-NMR\;and\;^{13}C-NMR$.

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The Study of Curcuma Longs L. Dyeing (울금의 염색성에 관한 연구)

  • 주영주;소황옥
    • Journal of the Korean Society of Clothing and Textiles
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    • v.20 no.3
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    • pp.429-437
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    • 1996
  • This paper surveys extraction condition-temperature, pH, and changes of adsorbance in accordance with extraction condition of curcuma Longa L. solution. Using extracted curcuma Longa L. Solution, dyeing behaviors with natural fiber, effect of mordants to color fastness and color change were investigated. It was found that the amount of absorption (K/S value) was increased by mordants (Fe, Cr) and mordants treatment affected color change of dyed fabric. Among the mordants, effect of citric acid to color change of dyed fabric was the smallest, and color difference of post- mordant treatment is smaller than that of pee-mordant treatment in making use of citric acid as mordant. And K/S value of post mordant was higher than that of pre mordant. It was found that the pre - and post- mordants treatment little affected color fastness, but affected the amound of adsorption and color change of dyed fabric.

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