• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 2004년도 Annual Meeting BioExibition International Symposium
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• pp.278-281
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• 2004

The $\alpha$1,6-mannosyltransferase encoded by Saccharomyces cerevisiae OCH1 plays a key role for the outer chain initiation of the N-linked oligosaccharides. A search for Hansenula polymorpha genes homologous to S. cerevisiae OCHI (ScOCH1) has revealed seven open reading frames (ORF100, ORF142, ORF168, ORF288, ORF379, ORF576, ORF580). All of the seven ORFs are predicted to be a type II integral membrane protein containing a transmembrane domain near the amino-terminal region and has a DXD motif, which has been found in the active site of many glycosyltransferases. Among this seven-membered OCH1 gene family of H. polymorpha, we have carried out a functional analysis of H. polymorpha ORF168 (HpOCH2) showing the highest identity to ScOCH1. Inactivation of this protein by disruption of corresponding gene resulted in several phenotypes suggestive of cell wall defects, including hypersensitivity to hygromycin B and sodium deoxycholate. The structural analysis of N-glycans synthesized in HpOCH2-disrupted strain (Hpoch2Δ) and the in vitro $\alpha$1,6-mannosyltransferase activity assay strongly indicate that HpOch2p is a key enzyme adding the first $\alpha$1,6-mannose residue on the core glycan Man$_{8}$GlcNAc$_2$. The Hpoch2Δ was further genetically engineered to synthesize a recombinant glycoprotein with the human compatible N-linked oligosaccharide, Man$_{5}$GlcNAc$_2$, by overexpression of the Aspergillus saitoi $\alpha$1,2-mannosidase with the 'HDEL” ER retention signal.gnal.

• 천문학회지
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• 제46권6호
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• pp.253-259
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• 2013

We present near-infrared light curves of HBC 722 after its the September 2010 outburst. We have been monitoring its near-infrared light curves since November 2010 with Korean Astronomy and Space Science Institute Infrared Camera System (KASINICS). HBC 722 exhibits large changes in optical and near-infrared brightness since its outburst. The J, H, and $K_s$ light curves over about 2.5 years show that in all observed bands HBC 722 progressively became fainter until around April 2011, down to J ~10.7, H ~9.9, $K_s$ ~9.3, but it is getting brighter again. Large scatter in the obtained light curve prevents us from finding whether there is any short timescale variation as reported in other optical observations. The near-infrared color of HBC 722 is becoming bluer since its outburst. The pre-outburst Spectral Energy Distribution (SED) of HBC 722 is consistent with that of a slightly reddened Class II YSO with the exception of the extraordinary IR-excess in the far-infrared region.

• 천문학회보
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• 제41권2호
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• pp.55.5-56
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• 2016

Turbulence plays an important role in molecular clouds. However, the properties of turbulence are poorly understood. In order to study the influence of turbulence in molecular clouds, we need to sample the turbulent properties in the full range of scales down to sonic scale. We mapped the $20^{\prime}{\times}60^{\prime}$ area covering the Orion Molecular Cloud (OMC) 1-4 region in HCN 1-0 and HCO+ 1-0 with Taeduk Radio Astronomy Observatory (TRAO) 14-m telescope as part of the TRAO key science program, "Mapping turbulent properties of star-forming molecular clouds down to the sonic scale (PI: Jeong-Eun Lee)". In addition, we combine our TRAO data with other molecular line maps ($^{13}CO$ 1-0, $C^{18}O$ 1-0, CS 1-0, $N_2H^+$ 1-0) obtained with the Nobeyama Radio Observatory (NRO) 45-m telescope. To analyze these data, we apply statistical methods, the principal component analysis (PCA) and spectral correlation function (SCF), which are known to be useful to study underlying turbulent properties and to quantitatively characterize cloud structure. We will present the preliminary results of observations and analyses.

• 천문학회보
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• 제42권2호
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• pp.43.3-43.3
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• 2017

NGC 6822 is a dwarf irregular galaxy in the Local Group and it is located in 500 kpc, further than the Large Magellanic Cloud and the Small Magellanic Cloud. Therefore, we can study star-forming processes by local condition in NGC 6822 instead of tidal force of the Galactic gravitational field. Hubble V is the brightest of several H II complexes in this galaxy. We observed Hubble V by using IGRINS attached on the 2.7 m telescope at the McDonald Observatory in Texas, US in May 2016. We performed a spectral mapping of $15^{{\prime}{\prime}}{\times} 7^{{\prime}{\prime}}$area on H and K bands, and detected emission lines of bright $Br{\gamma}\;{\lambda}2.1661{\mu}m$ and weak He I ${\lambda}2.0587{\mu}m$. Molecular hydrogen lines of 1-0S(1) ${\lambda}2.1218{\mu}m$, 2-1 S(1) ${\lambda}2.2477{\mu}m$, and 1-0 S(0) ${\lambda}2.2227{\mu}m$ was also detected. These emission lines show the structure of an ionized core and excited surface of clouds by far-ultraviolet photons, photodissociation region (PDR). We present three-dimensional maps of emission line distributions through multi slit scanning data and compare these results with the previous study. This presentation shows the physical structure of the star-forming regions and we discuss a PDR model and an evolution of Hubble V complex.

• 한국재료학회지
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• 제16권12호
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• pp.761-765
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• 2006

[ $II-III_2-(S,Se)_4$ ] structured of phosphor has been used at various field because those have high luminescent efficiency and broad emission band. Among these phosphors, the europium doped $BaGa_2S_4$ was prepared by solid-state method and had high potential application due to an emissive property of UV region. Also, the common sulfide phosphors were synthesized by using injurious $H_2S\;or\;CS_2$ gas. However, in this study $BaGa_2S_4:Eu^{2+}$ phosphor in addition to excess sulfur was prepared under at 5% $H_2/95%\;N_2$ reduction atmosphere. Thus, this process could be considered as large scale synthesis because of non-harmfulness and simplification. The photoluminescence efficiency of the prepared $BaGa_2S_4:Eu^{2+}$ phosphor increased 20% than that of commercial $SrGa_2S_4:Eu^{2+}$ phosphor. The prepared $BaGa_2S_4:Eu^{2+}$ could be applied to green phosphor for white LED of three wavelengths.

• 대한방사선치료학회지
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• 제15권1호
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• pp.53-60
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• 2003

I. 목적 방사선치료를 함에 있어서 체내의 선량분포를 알기 위해서는 정확한 신체 단면도 제작이 반드시 필요하다. 최근에는 종양과 주변부위와의 관계가 명확하게 나타나는 C-T 영상이 치료계획에 많이 이용되고 있다. 하지만 아직도 석고나 납줄등을 이용하여 수작업으로 신체 단면도를 그리는 경우가 대부분이다. 그러나 이러한 방법은 옮기는 과정에서의 오류등으로 정확성의 신뢰도에 문제가 있고 소요시간이 길다는 단점이 있어 이를 개선하기 위해 digital 영상을 이용한 신체 단면도 제작을 시도해 보았다. II 대상 및 방법 digital camera를 이용하여 center line의 axial laser가 지나가는 면의 caudal쪽에서 center방향의 사진과 동일지점에서 QA용 phantom의 사진을 촬영한 후 image를 scanning한다. 삼각함수의 기본적인 원리를 이용하여 오차 범위를 최소화하였으며 각 지점의 X, Y좌표를 구하여 실제적인 크기의 신체 단면도를 완성하여 보았으며 여러 가지의 모양의 sample용 phantom과 실제 chest의 환자에게 적용하여 보았다. III. 결과 및 결론 기존의 납줄을 이용하여 제작한 신체단면도와 digital영상을 이용하여 제작한 신체단면도를 비교, 검토한 결과 오차범위가 실험적 오차에 있을 정도로 큰 차이가 없어 시간에 구애받지 않고 제작할 수 있는 이 방법이 보다 더 효율적이였다. 하지만 H&N등 요철이 심한 경우에 사용할 경우에는 보다 더 세심한 주의가 필요하며 영구적으로 사용하기 위해서는 두 개 이상의 camera 영상을 이용하는 photogrammatory기법의 도입 등, system적으로 좀더 수정 보완되어야 할 것으로 사료된다.

• 한국환경생태학회지
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• 제25권3호
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• pp.267-283
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• 2011

내장산국립공원지역에서 확인된 환경부 지정 멸종위기종은 진노랑상사화, 노랑붓꽃, 대흥란 및 백운란 4분류군이었으며, 식물구계학적 특정식물종은 총 159분류군으로 I 등급에 해당하는 분류군은 옥녀꽃대, 가는갈퀴나물 및 말오줌때등으로 91분류군, II 등급에 해당하는 분류군은 참개별꽃, 퍼진고사리 및 토끼고사리 등으로 17분류군, III 등급에 해당하는 분류군은 고려엉겅퀴, 어리병풍 및 솜분취 등으로 32분류군, IV 등급에 꼬리말발도리, 갈사초 및 측백나무 등으로 9분류군, V 등급에 백양더부살이, 진노랑상사화 및 백양꽃 등으로 10분류군이 확인되었다. 한국고유생물종은 애기닥나무, 참개별꽃 및 가는장구채 등으로 총 40분류군이 확인되었다. 굴거리나무림(제 91호)와 비자나무림(제 153호)이 천연기념물로 지정되어 보호되고 있다. 귀화식물은 닭의덩굴, 털여뀌 및 소리쟁이 등으로 30분류군이 확인되었고, 생태계교란야생식물은 나타나지 않았다.

• IMMUNE NETWORK
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• 제1권1호
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• pp.61-69
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• 2001

Background: To determine the molecular structure of type II collagen-specific T-cell receptors associated with rheumatoid arthritis (RA). Methods: We generated CII-specific T-cell lines of 8 RA patients by prolonged in vitro culture with bovine CII (bCII) and the immunogenic peptide (256-270) of human CII. The proliferation response towards CII stimulation was measured from the uptake of 3H-thymidine. Changes in the secretion of Th 1 and Th2 cytokines in the culture supernatent were measured by ELISA. The TCR clonotypes of these T-cells were examined by RT-PCR/SSCP analyses of all 22 $V_{\beta}$ chains. Results: T-cells from patients' tissue exhibited strong proliferation index upon CII stimulation, which was maintained up to 6 months in the culture. The secretion of INF-$\gamma$from these T-cells increased along with the duration of culture time, while the amount of IL-4 production did not show significant changes. The SSCP band patterns of patients' T-cells appear as discrete bands unlike the smeary streak produced from normal samples. Some SSCP bands, each representing selected expansion of a TCR containing certain subtype of $V_{\beta}$ peptides, appeared to be identical in more than one patients. Among these, the expansion of SSCP band representing the $V_{\beta}$ 14 CDR3 region persisted after switching the antigen to the immunogenic human peptide (256-270). Conclusion: CII-reactive T-cells expressing distinct CDR3 motifs are selectively expanded in the peripheral blood and synovial fluid of RA patients, and their persistent proliferation upon CII stimulation, as well as the production Th 1-type cytokines, may play pivotal roles in RA pathogenesis.

• Korean Chemical Engineering Research
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• 제55권3호
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• pp.353-357
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• 2017

본 연구에서는 THF + 3-OH THF + $CH_4$ + $H_2O$ 시스템에서의 크러스레이트 하이드레이트의 형성 과정과 이에 따른 상 거동을 열역학 및 분광학적 방법을 통해 분석하였다. 정적 반응기에서의 온도 변화에 따라 THF와 3-OH THF를 포함하는 유기 분자들의 큰 동공 내 점유에 의해 크러스레이트 하이드레이트가 형성 및 해리되는 현상을 압력 변화를 관찰함으로써 확인하였다. 또한, 이들 유기 분자의 상대 조성에 따라 크러스레이트 하이드레이트가 안정적으로 존재할 수 있는 상평형 영역이 순수 메탄 하이드레이트 대비 보다 낮은 압력 및 높은 온도 조건으로 이동될 수 있음을 확인하였다. 엑스선 회절 분광 분석을 통해 이들 조성에서의 크러스레이트 하이드레이트는 구조-II를 형성 하는 것을 확인하였으며, 라만 분광 분석을 통해 구조-II의 큰 동공과 작은 동공에 각각 메탄이 점유되어 있음을 또한 확인하였다.

• Parasites, Hosts and Diseases
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• 제58권6호
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• pp.709-714
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• 2020

Knockdown resistance (kdr) mutations in the voltage-gated sodium channel (VGSC) of mosquitoes confer resistance to insecticides. Although insecticide resistance has been suspected to be widespread in the natural population of Aedes aegypti in Myanmar, only limited information is currently available. The overall prevalence and distribution of kdr mutations was analyzed in Ae. aegypti from Mandalay areas, Myanmar. Sequence analysis of the VGSC in Ae. aegypti from Myanmar revealed amino acid mutations at 13 and 11 positions in domains II and III of VGSC, respectively. High frequencies of S989P (68.6%), V1016G (73.5%), and F1534C (40.1%) were found in domains II and III. T1520I was also found, but the frequency was low (8.1%). The frequency of S989P/V1016G was high (55.0%), and the frequencies of V1016G/F1534C and S989P/V1016G/F1534C were also high at 30.1% and 23.5%, respectively. Novel mutations in domain II (L963Q, M976I, V977A, M994T, L995F, V996M/A, D998N, V999A, N1013D, and F1020S) and domain III (K1514R, Y1523H, V1529A, F1534L, F1537S, V1546A, F1551S, G1581D, and K1584R) were also identified. These results collectively suggest that high frequencies of kdr mutations were identified in Myanmar Ae. aegypti, indicating a high level of insecticide resistance.