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Seed Dormancy Type and Germination Characteristics in Tiarella polyphylla D. Don Native to Korea (한반도 자생식물 헐떡이풀 종자의 휴면유형과 발아특성)

  • Choi, Han;Lee, Seung Youn;Rhie, Yong Ha;Lee, Jeong Ho;Kim, Sang Yong;Lee, Ki Cheol
    • Korean Journal of Plant Resources
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    • v.31 no.4
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    • pp.363-371
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    • 2018
  • Tiarella polyphylla D. Don is a native plant distributed only in Ulleung Island in Korea and has been traditionally used for medicinal purposes, although it is also used ornamentally. This study was conducted to determine the requirements for dormancy break and germination and to classify the type of seed dormancy. The experiments were performed with cold stratification (0 or 12 weeks at $5^{\circ}C$), warm stratification (0, 4, 8, or 12 weeks at $23^{\circ}C$, followed by 8 weeks at $5^{\circ}C$, and then incubation at $23^{\circ}C$), and $GA_3$ treatments (0, 10, 100, or 1000 mg/L). The treated seeds were incubated on aseptic media at room chamber ($23^{\circ}C$, a 16h photoperiod of fluorescent lamps with $40{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$). The seeds were dispersed in nature as underdeveloped embryos with no physical barrier to absorb water to prevent water absorption. However, the seeds did not germinate for 30 days after sowing without any pre-treatments. Thus, the seeds had morphological dormancy (MD) and physiological dormancy (PD). The final germination percentage following cold stratification (0 or 12 weeks) was 66.7% and 45.6%, respectively. The cold stratification delayed seed germination by about 3 weeks. In the warm stratification experiment (0, 4, 8, or 12 weeks), the final germination percentage was 21.1%, 27.8%, 41.1%, and 57.8%, respectively, 20 weeks after sowing. The embryos of the T. polyphylla seed grew in relatively warm temperatures ($23^{\circ}C$). $GA_3$ application overcame seed dormancy and promoted germination. Following $GA_3$ treatment (0, 10, 100, or 1000 mg/L), the final germination percentage was 33.3%, 45.0%, 42.5%, and 72.5%, respectively. These results suggest that the T. polyphylla seeds had non-deep simple morphophysiological dormancy (MPD) and $GA_3$ treatment could be used as a substitute for warm stratification for breaking seed dormancy. To our knowledge, this is the first report of seed dormancy characteristics of the genus Tiarella native to Korea.

Study on the Influence of Ca and Mg Saturation Ratios of Soil on the Uptake of Ca, Mg, and K by Rice Plant (답토양(畓土壤)의 Ca, Mg 포화비(飽和比)가 수도(水稻)의 Ca, Mg, K의 흡수(吸收)에 미치는 영향(影響))

  • Jeong, Y.G.;Hong, J.W.;Ha, H.S.
    • Korean Journal of Soil Science and Fertilizer
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    • v.20 no.2
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    • pp.115-121
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    • 1987
  • A pot experiment was conducted to investigate the influence of Ca and Mg saturation ratios of soil on the uptake of Ca, Mg and K by rice plant. A silty loam soil was treated with $CaCl_2$ and $MgCl_2$ to obtain different degrees of Ca and Mg saturation. The studied ranges of Ca and Mg saturation ratios were 81:19, 70:30, 52:48, 55:45, and 31:69 in terms of the ratio of exchangeable Ca and Mg. Two levels of K application (90kg/ha, and 180kg/10a as $K_2O$) were also included in this study. The significant observation were summarized as follows. 1. When the Ca saturation of soil was dominant over Mg, the soil solution contained more Ca than Mg and vice versa. These led to the higher uptake of Ca by rice plant in Ca dominant soils and higher uptake of Mg in Mg dominant soils. 2. When the Ca and Mg saturation ratio was about equal, more Mg was released to soil solution leading to higher concentration of Mg in rice plant compare to that of Ca. 3. A trend was observed that the concentration of K in soil solution was lower in Mg dominant soils than in Ca dominant soils. This also resulted in the depressed uptake of K by rice plant under Mg dominant system when compare to Ca dominant system. 4. The increase application of K led to the increase in relative concentration of K to (Ca+Mg+K), and to the depression of divalent uptake by rice plant. However, it was observed that the degree of depression in uptake divalent by K application was more sensitive in case of Mg than that of Ca. 5. When viewed from grain yield of rice, it is pointed out that the optimum range of Ca to Mg ratio in soil may fall in the vicinity of 7:3. 6. Although K uptake by rice plant was influenced by the term of $AK^+/{\sqrt{A(Ca^{{+}{+}}+Mg^{{+}{+}})$ in soil solution, $AK^+$ itself was affected by the ratios of Ca:Mg in soil, as it were $AK^+$ value was decreased in Mg dominant soil than in Ca.

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Effects of Rhizobium Inoculation on the changes of Ureide-N and Amide-N Concentration in Stem and Root exudate of Soybean Plant (대두근류균(大豆根瘤菌) 접종(接種)이 뿌리와 줄기 즙액중(汁液中) Amide-N 및 Ureide-N 농도(濃度)에 미치는 영향(影響))

  • Ko, Jae-Young;Suh, Jang-Sun;Lee, Sang-Kyu
    • Korean Journal of Soil Science and Fertilizer
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    • v.22 no.4
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    • pp.329-336
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    • 1989
  • A series of green house experiment was conducted to find but the effect of fertilizer application and inoculation of rhizobium on the changes of amide-N, ureide-N and $NH_4-N$ concentration in stem and root exudates of soybean plant growth. The results obtained were summarized as follows ; 1. Five strains of indigenous Rhizobium japonicum-nitrogen fixing activity($C_2H_2$-reducing activity) was more than 6.4 to 20.1 nmole/hr/tube-were identified from 37 soil samples in 22 areas of farmers field throughout country. 2. These identified 5 strains of rhizobium were obtained high nitrate reductase but low ammonium and nitrite oxidase activities. Among 5 strains of rhizobium the Rhizobium japonicum RjK-134 was applied for this green house experiment. 3. Dry matter yield was increased by the combination of inoculation of Rhizobium japonicum RjK-134 with no fertilizer and without nitrogen fertilizer application. However, dry matter yield was decreased with application of N and NPK with inoculation of rhizobium. 4. The concentrations of amide-N and ureide-N were increased in xylem sap than that of root exudate and higher concentration was obtained ar 30 days after planting than flowering stage (45 days after planting). 5. The combination of NPK application with inoculation of Rhrizobium japonicum RjK-134 enhanced the increase of amide-N and ureide-N concentration in xylem sap and root exudate. 6. High ammonium-N concentration in xylem sap and root exudate were obtained in combination with without-fertilizer under no inoculation of rhizobium and N and NPK application with inoculation of rhizobium.

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Assessment of Fertilizer Efficiency of Pharmaceutical Byproduct and Cosmetic Industry Wastewater Sludge as Raw Materials of Compost (제약업종 부산물 및 화장품 제조업 폐수처리오니의 비효검정)

  • Lim, Dong-Kyu;Kwon, Soon-Ik;Lee, Seung-Hwan;So, Kyu-Ho;Sung, Ki-Suk;Koh, Mun-Hwan
    • Korean Journal of Soil Science and Fertilizer
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    • v.38 no.2
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    • pp.108-117
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    • 2005
  • Pharmaceutical byproduct sludge and cosmetic industry wastewater sludge can be used as a raw material of compost. Effects of three types of pharmaceutical byproduct sludge and one type of cosmetic industry wastewater sludge on soil properties and red pepper growth were investigated in a field based concrete pot ($2{\times}2m$). These sludges and pig manure ($5Mg\;ha^{-1}$, dry basis) were incorporated into the upper of loam soil 30 days prior to transplanting red pepper. Changes in soil properties and contents of heavy metals and toxic organic compounds in soil and plant were measured. And also plant growth measurement and bioassay of soil phytotoxicity were included. Contents of heavy metals were increased in the soils treated with the sludges. Plant growth in the sludge treatments were mostly inferior to that of NPK treatment, especially in early stage. Content of N in plant was lower in all sludge treatments at early and middle growth stages, and it was especially caused by characteristics and concentration of nitrogen and organic matter of sludges. Total yield of red pepper was highest in the NPK treatment and followed by pharmaceutical sludge 3, pig manure, pharmaceutical sludge 1, and pharmaceutical sludge 2, and the yield of cosmetic sludge treatment was considerably lower than others. HEM and PAHs contents in soil of cosmetic sludge treatment were $4.80mg\;kg^{-1}$ and $2,263.2{\mu}g\;kg^{-1}$, respectively. Root elongation of lettuce exposed to the water extract of soil treated with cosmetic sludge was about 20% of that found in the test with soil extract of non fertilization treatment. At present, raw materials of compost were authorized according to the contents of organic matter, heavy metals and product processing. Toxic organic compounds analysis and bioassay would be helpful for authorization and assessment of suitability of raw materials of compost.

Studies on the Various Utilization of Microbial Formulation for the Production of Vegetable Crops (원예작물(園藝作物) 생산성(生産性)에 미치는 미생물(微生物) 제제(製劑)의 복합적(複合的) 이용연구(利用硏究))

  • Kim, Kwang-Sik;Kim, Young-Woong;Choi, Young-Soo
    • Korean Journal of Soil Science and Fertilizer
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    • v.28 no.2
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    • pp.191-205
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    • 1995
  • The carrier materials used for the development of bacterial inoculants to be effective in field were made with various carrier materials of two major forms, alginate bead and powder inoculants. Inoculants were prepared after mixing those carrier materials with Pseudomonas fluorescens SSL3 and Bacillus subtilis B5, and the treatment effects of each inoculants was investigated on cucumber, tomato, pepper and potato. Survival density of SSL3 and B5 in various carrier materials for duration of storage and the bead inoculants were better than the powder. In the powders, survival rate increased in carrier materials treated 5% skimilk. The growth condition of microorganisms in carrier materials is good at powder. When they were preserved in the long period, contamination is problem. Scanning(200 to 600nm) of the P. fluorescens SSL3 supernatant in centrifuged MKB broth incubated for 48h had two main peaks, pyochelin(300nm) and pyoverdin(400nm). The potato yield in field experiments of spring, treated with bead formulas showed increase of 22~29% in whole potato breeds as compared with control, because the bead formulas degraded, and released the antibiotic microorganisms in slow and constant rate. In the pot experiment, there were significant difference in soil, wheatbran, and bead formed wheatbran.

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Compaction Characteristics of Multi-cropping Paddy Soils in South-eastern Part of Korea (우리나라 동남부 다모작 논토양의 경반화 특성)

  • Yun, Eul-Soo;Jung, Ki-Yeul;Park, Ki-Do;Sonn, Yeon-Kyu;Park, Chang-Yeong;Hwang, Jae-Bog;Nam, Min-Hee
    • Korean Journal of Soil Science and Fertilizer
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    • v.44 no.5
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    • pp.688-695
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    • 2011
  • This study was carried out for some survey about soil compaction in the multi-cropping system of paddy field. Investigated sites were 90 farmer's fields in south-eastern part of Korea. The tillage practices season was different according to cropping system of paddy; in spring for mono rice cultivation and in autumn for the multi-cropping field. The average tillage depth in investigated sites was about 25 cm, however, it is different between the farmer's tillage practices and soil characteristics. It is high correlation to tillage deep and minimum resistance of penetration. The reaching soil deep to maximum resistance of penetration was about 27 cm, and average penetration resistance of the deep is 1.8~2.0 MPa for moderately fine-textured soils and more than 3.0 MPa for moderately coarse-textured soils. The difference of penetration resistance between cultivating and compacted layer was in order to sandy loam > clayey loam > clayey, and the difference was lesser in poorly drained soils than somewhat poorly ones. In the rice mono cropping field, the maximum resistance in no-tillage for 15 years was 1.18~1.25 Mpa at 20~25 cm in soil deep, however, the resistance of field with every year tillage practices was 2.03~2.21 Mpa. In the extremely compacted sandy loam textured soils, the penetration resistance at 30 cm in soil depth was drastically reduced by the subsoil from 5.2 Mpa to 3.2 Mpa, and the watermelon root in plastic film house was deep elongated.

Effect of Swine Liquid Manure on Soil Chemical Properties and Growth of Rice (Oryza sativa L.) (양돈분뇨 발효액비 시용이 토양 화학성과 벼 (Oryza sativa L.) 생육에 미치는 영향)

  • Lee, Kyu-Hoi;Yoo, Jae-Hong;Park, Eun-Ju;Jung, Yeong-In;Tipayno, S.C.;Shagol, C.C.;Sa, Tong-Min
    • Korean Journal of Soil Science and Fertilizer
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    • v.43 no.6
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    • pp.945-953
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    • 2010
  • This study was conducted to evaluate the effect of swine liquid manure (SLM) on rice grown in Yeonggwang-gun in 2008. The treatments consisted of SLM and chemical fertilizer (CF) based on the recommended amount of nitrogen (11 kg N $10a^{-1}$). The Total N content of the SLM used was 2,881 mg $L^{-1}$. Plant height at the early stage of growth and tiller number were not significantly different between plots applied with swine liquid manure and those with chemical fertilizer in all areas. Plant height at the later stage of growth, lodging and yield were not significantly different between plots applied with swine liquid manure and those with chemical fertilizer in three areas (Baeksu, Gunnam, Beopseong). Plant height at the later stage of growth, as well as lodging were higher in SLM plots than in chemically fertilized plots in Yeonggwang and Yeomsan. However, grain yield was lower in SLM plots than in chemically fertilized plots in these areas. Soil organic matter content and exchangeable cations increased in the swine liquid manure applied plots. Moreover, heavy metal content did not increase in the plots treated with swine liquid manure. Further research to determine the suitable rate of swine liquid manure is needed to reduce lodging damage and to increase the yield and quality of rice.

Analyzing the Characteristics of Atmospheric Stability from Radiosonde Observations in the Southern Coastal Region of the Korean Peninsula during the Summer of 2019 (라디오존데 고층관측자료를 활용한 한반도 남해안 지역의 2019년도 여름철 대기 안정도 특성 분석)

  • Shin, Seungsook;Hwang, Sung-Eun;Lee, Young-Tae;Kim, Byung-Taek;Kim, Ki-Hoon
    • Journal of the Korean earth science society
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    • v.42 no.5
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    • pp.496-503
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    • 2021
  • By analyzing the characteristics of atmospheric stability in the southern coastal region of the Korean Peninsula in the summer of 2019, a quantitative threshold of atmospheric instability indices was derived for predicting rainfall events in the Korean Peninsula. For this analysis, we used data from all of the 243 radiosonde intensive observations recorded at the Boseong Standard Weather Observatory (BSWO) in the summer of 2019. To analyze the atmospheric stability of rain events and mesoscale atmospheric phenomena, convective available potential energy (CAPE) and storm relative helicity (SRH) were calculated and compared. In particular, SRH analysis was divided into four levels based on the depth of the atmosphere (0-1, 0-3, 0-6, and 0-10 km). The rain events were categorized into three cases: that of no rain, that of 12 h before the rain, and that of rain. The results showed that SRH was more suitable than CAPE for the prediction of the rainfall events in Boseong during the summer of 2019, and that the rainfall events occurred when the 0-6 km SRH was 150 m2 s-2 or more, which is the same standard as that for a possible weak tornado. In addition, the results of the atmospheric stability analysis during the Changma, which is the rainy period in the Korean Peninsula during the summer and typhoon seasons, showed that the 0-6 km SRH was larger than the mean value of the 0-10 km SRH, whereas SRH generally increased as the depth of the atmosphere increased. Therefore, it can be said that the 0-6 km SRH was more effective in determining the rainfall events caused by typhoons in Boseong in the summer of 2019.

Chemical components and hepato-protective effect of Lentinula edodes fermented by lactic acid bacteria (표고 유산균 발효물의 성분 및 간기능 보호 효과)

  • Im, Seung-Bin;Kim, Kyung-Je;Jin, Seong-Woo;Koh, Young-Woo;Ha, Neul-I;Jeong, Hee-Gyeong;Lee, Jae-Keun;Yun, Kyeong-Won;Seo, Kyoung-Sun
    • Journal of Mushroom
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    • v.19 no.3
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    • pp.191-199
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    • 2021
  • This study was conducted to improve the useful components and biological activity of Lentinula edodes fermented by lactic acid bacteria (LAB). Three LAB strains (Lactobacillus brevis KCCM 11904, L. plantarum KCCM 354469, and L. fermentum KCCM 12116) were inoculated and used for L. edodes hot water extract (10%, 20%, 30%) fermentation. LAB fermentation of L. edodes hot water extracts decreased pH and thus were more acidic than non-fermented L. edodes hot water extract. β-glucan and ergothioneine contents were increased by L. edodes in a concentration-dependent manner. The ergothioneine and β-glucan contents were highest in fermented with 30% L. edodes hot water extract fermented by L. plantarum and L. brevis (40.48 mg/100 g and 13.94%, respectively). The hepato-protective effect of fermented L. edodes hot water extracts by the three LAB were tested using Sprague-Dawley rat primary hepatocytes. In primary hepatocytes obtained following liver injury induced by acetaminophen, fermented L. edodes hot water extracts by the three LAB showed protective effects, as evident by reduction of the aspartate aminotransferase, alanine aminotransferase, and lactate dehydrogenase liver markers. The collective results indicate that the fermented L. edodes hot water extracts obtained using LAB are potentially valuable in preventing or treating liver disease.

Effect of Reversible Air-circulation Fans on Air Uniformity in a Cultivation Facility for Oyster Mushroom (느타리재배사 정역 제어 대류팬이 공기 균일도에 미치는 영향)

  • Yum, Sung Hyun;Kim, Si Hwan
    • Journal of Bio-Environment Control
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    • v.30 no.4
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    • pp.383-392
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    • 2021
  • It has been known that oyster mushrooms cultivated in facilities with thermal insulation have been strongly affected by inner environments. Forced air-circulation fans exert much direct influence on disturbing air inside the facility so the matter is of particular interest. This study is carried out to investigate the measured levels of air uniformity in a cultivation facility for oyster mushroom in the various cases that reversibly controlled air-circulation fans which drove the flow in the upward and reverse direction by turn and unidirectional fans by which the wind blew upwards only were operated from July 1 to 10. The actual survey for the selection of ongoing operation cases presented that farmers, even though there were some discrepancies, have made use of fans in a way that it paused for 5-30min after running for 5-15min by turn. The level of air uniformity in the case of adopting reversible fans revealed a slight difference of 1.4-1.8℃ (Temp.) and 7.8-8.7% (R.H.) under the condition of not using a cooler during the investigation period. By contrast, unidirectional fans showed a noticeable difference of 3.2-3.7℃ and 14.0-15.4%, which meant that air uniformity driven by reversible fans much more increased compared to that for unidirectional fans. Among the twenty operational applications considered for reversible fans, the circumstance that the wind blew upwards for 10-15min and ceased for 5-10min and blew again in the reverse direction for 10-15min in succession gave minor improvements at the level of air uniformity, but at present there was somewhat difficult to make decision on which cases were optimally best. It seems necessary that the effects of reversible fans on air uniformity as well as qualities of oyster mushrooms have to be appraised in the cultivation period and the flow visualization needs to be done to ascertain the performance of air mixture.