• Title/Summary/Keyword: Growth limiting factor

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Effect of Brevibacterium iodinum RS16 and Methylobacterium oryzae CBMB20 Inoculation on Seed Germination and Early Growth of Maize and Sorghum-sudangrass hybrid Seedling under Different Salinity Levels

  • Kim, Ki-Yoon;Hwang, Seong-Woong;Saravanan, Venkatakrishnan Sivaraj;Sa, Tong-Min
    • Korean Journal of Soil Science and Fertilizer
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    • v.45 no.1
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    • pp.51-58
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    • 2012
  • Salinity is one of the most relevant abiotic factor limiting crop yield and its net primary productivity. In addition, salinity induces an increased stress ethylene synthesis in plants which, in turn, exacerbate the responses to the stressor. Bacterial single or co-inoculation effect was tested using previously characterized plant growth promoting (PGP) bacteria Brevibacterium iodinum RS16 and Methylobacterium oryzae CBMB20 on maize and sorghum-sudan grass hybrid under different concentrations of NaCl. Non-inoculated maize and sorghum-sudangrass hybrid showed 33.4% and 20.0% reduction in seed germination under highest NaCl (150 mM) level tested. However, under the same NaCl concentration, co-inoculation with B. iodinum RS16 and M. oryzae CBMB20 PGP strains increased the seed germination in maize (16.7%) and sorghum-sudangrass hybrid (4.4%). In Gnotobiotic growth pouch experiments conducted for maize and sorghum-sudangrass hybrid, co-inoculation of PGP B. iodinum RS16 and M. oryzae CBMB20 mitigated the salinity stress and promoted root length by 22.9% and 29.7%, respectively. Thus the results of this study could help in development of potential bioinoculants that may be suitable for crop production under saline conditions.

Root metabolic cost analysis for root plasticity expression under mild drought stress

  • Kano-Nakata, Mana;Mitsuya, Shiro;Inukai, Yoshiaki;Yamauchi, Akira
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.328-328
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    • 2017
  • Drought is a major limiting factor that reduces rice production and occurs often especially under recent climate change. Plants have the ability to alter their developmental morphology in response to changing environment, which is known as phenotypic plasticity. In our previous studies, we found that one chromosome segment substitution line (CSSL50 derived from Nipponbare and Kasalath crosses) showed no differences in shoot and root growth as compared with the recurrent genotype, Nipponbare under non-stress condition but showed greater growth responses compared with Nipponbare under mild drought stress condition. We hypothesized that reducing root respiration as metabolic cost, which may be largely a consequence of aerenchyma formation would be one of the key mechanisms for root plasticity expression. This study aimed to evaluate the root respiration and aerenchyma formation under various soil moisture conditions among genotypes with different root plasticity. CSSL50 together with Nipponbare and Kasalath were grown under waterlogged conditions (Control) and mild drought stress conditions (20% of soil moisture content) in a plastic pot ($11cm{\times}14cm$, ${\varphi}{\times}H$) and PVC tube ($3cm{\times}30cm$, ${\varphi}{\times}H$). Root respiration rate was measured with infrared gas analyzer (IRGA, GMP343, Vaisala, Finland) with a closed static chamber system. There was no significant difference between genotypes in control for shoot and root growth as well as root respiration rate. In contrast, all the genotypes increased their root respiration rates in response to mild drought stress. However, CSSL50 showed lower root respiration rate than Nipponbare, which was associated by higher root aerenchyma formation that was estimated based on internal gas space (porosity) under mild drought stress conditions. Furthermore, there were significant negative correlations between root length and root respiration rate. These results imply that reducing the metabolic cost (= root respiration rate) is a key mechanism for root plasticity expression, which CSSL50 showed under mild drought.

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Control of ZnO Sputtering Growth by Changing Substrate Bias Voltage (ZnO 스퍼터링에서 기판전압의 변화에 의한 성장 조절)

  • Meng, Jun;Choi, Jaewon;Jeon, Wonjin;Jo, Jungyol
    • Journal of the Semiconductor & Display Technology
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    • v.16 no.2
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    • pp.94-97
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    • 2017
  • Amorphous Si has been used for data processing circuits in flat panel displays. However, low mobility of the amorphous Si is a limiting factor for the data transmission speed. Metal oxides such as ZnO have been studied to replace the amorphous Si. ZnO is a wide bandgap (3.3 eV) semiconductor with high mobility and good optical transparency. When ZnO is grown by sputtering with $O_2$ as an oxidizer, there can be many ion species arising from $O_2$ decomposition. $O^+$, $O_2{^+}$, and $O^-$ ions are expected to be the most abundant species, and it is not clear which one contributes to the ZnO growth. We applied alternating substrate voltage (0 V and -70 V) during sputtering growth. We studied changes in transistor characteristics induced by the voltage switching. We also compared ZnO grown by dc and rf sputtering. ZnO film was grown at $450^{\circ}C$ substrate temperature. ZnO thin-film transistor grown with these methods showed $7.5cm^2/Vsec$ mobility, $10^6$ on-off ratio, and -2 V threshold voltage.

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Using Trophic State Index (TSI) Values to Draw Inferences Regarding Phytoplankton Limiting Factors and Seston Composition from Routine Water Quality Monitoring Data (영양상태지수 (trophic state index)를 이용한 수체 내 식물플랑크톤 제한요인 및 seston조성의 유추)

  • Havens, Karl E
    • Korean Journal of Ecology and Environment
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    • v.33 no.3 s.91
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    • pp.187-196
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    • 2000
  • This paper describes a simple method that uses differences among Carlson's (1977) trophic state index (TSI) values based on total phosphorus (TP), chlorophyll a (CHL) and Secchi depth (SD) to draw inferences regarding the factors that are limiting to phytoplankton growth and the composition of lake seston. Examples are provided regarding seasonal and spatial patterns in a large subtropical lake (Lake Okeechobee, Florida, USA) and inter- and intra-lake variations from a multilake data set developed from published studies. Once an investigator has collected routine water quality data and established TSI values based on TP, CHL, and SD, a number of inferences can be made. Additional information can be provided where it also is possible to calculate a TSI based on total nitrogen (TN). Where TSI (CHL)<>TSI (SD), light attenuating particles are large (large filaments or colonies of algae), and the phytoplankton may be limited by zooplankton grazing. Other limiting conditions are inferred by different relationships between the TSI values. Results of this study indicate that the analysis is quite robust, and that it generally gives good agreement with conclusions based on more direct methods (e.g., nutrientaddition bioassays, zooplankton size data, zooplankton removal experiments). The TSI approach, when validated periodically with these more costly and time-intensive methods, provides an effective, low cost method for tracking long-term changes in pelagic structure and function with potential value in monitoring lake ecology and responses to management.

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Growth Efficiency and Thermal Stress in Panax ginseng Grown at Various Temperatures under Dark (온도별 암하생장시 인삼의 생장효율과 열장애)

  • Park, Hoon;Yoo, Ki-Jung;Cooi, Byung-Ju
    • Journal of Ginseng Research
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    • v.12 no.1
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    • pp.1-10
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    • 1988
  • Panax ginseng seedlings were grown at various temperature regimes from 15 to $30^{\circ}C$ for 19 days under dark and the linear relationship between various regrowth efficiencies and thermal stress indices, cumulative superoptimum temperatures corrected with factors. Gross growth efficiency(shoot weight l root weight loss) was 37.5 % at the optimum temperature $15^{\circ}C$/$15^{\circ}C$, and 12.3% at the highest temperature, $30^{\circ}C$/$30^{\circ}C$ while net growth efficiency (shoot weight + Sm)l(root loss-Rm), which corrected by maintenance respiration for shoot(Sm) and root(Rm) was 39.6% and 16.7 at optimum and highest temperature respectively. All growth efficiencies showed negative correlations (p = 0.001) with all thermal stress indices and negative(p = 0.001) with shoot growth(St). When growth temperature difference in a day was nil or above $15^{\circ}C$ growth efficiency decreased greatly. Thermal stress indices showed negative correlation with root dry matter loss(RDL) but positive with Rm. St showed positive correlation with RDL. Thermal stress appeared to inhibit substrate supply for shoot growth resulting in the extremely low growth efficiency comparing with other crops that seems to be main rate limiting factor of slow growth, Thus it is necessary that growth efficiency and thermal stress must be elucidated in terms of metabolic pathway.

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Characteristics of the Bioreactors of Hydrogen-producing Immobilized Cells (II) -Overall Effectiveness Factor in Continuous Reactors- (수소생산 고정화 생물 반응기의 특성(II) -연속 반응기에서의 총괄 효율인자 -)

  • 이명재;선용호;한정우;조영일
    • Microbiology and Biotechnology Letters
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    • v.16 no.6
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    • pp.510-516
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    • 1988
  • The effects of input substrate concentration and dilution rate on mass transfer resistance in the operation of immobilized cell reactors were investigated using Rhodospirillum rubrum KS-301 immobilized by Ca alginate as reactor element and glucose as growth-limiting substrate. The kinetic parameters were obtained to estimate effectiveness factors. In the packed-bed reactor, internal mass transfer resistance was predominating although external resistance could not be neglected. The overall effectiveness factor was decreased with increase of dilution rate. In the continuous stirred-tank reactor, external resistance was nearly neglected and the overall effectiveness factor was not affected by dilution rate. In this experiment the overall effectiveness factors in PBR and CSTR were estimated to be 0.70 and 0.71 at D$_{i}$ = 0.2/h, R = 0.15 cm, and S$_{i}$ : 1.0g/L, respectively.

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Salt-induced Differential Gene Expression in Italian Ryegrass (Lolium multiflorum Lam.) Revealed by Annealing Control Primer Based GeneFishing approach

  • Lee, Ki-Won;Lee, Sang-Hoon;Choi, Gi Jun;Ji, Hee Jung;Hwang, Tae Young;Kim, Won Ho;Rahman, Md. Atikur
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.37 no.3
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    • pp.231-236
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    • 2017
  • Salt stress is one of the most limiting factors that reduce plant growth, development and yield. However, identification of salt-inducible genes is an initial step for understanding the adaptive response of plants to salt stress. In this study, we used an annealing control primer (ACP) based GeneFishing technique to identify differentially expressed genes (DEGs) in Italian ryegrass seedlings under salt stress. Ten-day-old seedlings were exposed to 100 mM NaCl for 6 h. Using 60 ACPs, a total 8 up-regulated genes were identified and sequenced. We identified several promising genes encoding alpha-glactosidase b, light harvesting chlorophyll a/b binding protein, metallothionein-like protein 3B-like, translation factor SUI, translation initiation factor eIF1, glyceraldehyde-3-phosphate dehydrogenase 2 and elongation factor 1-alpha. These genes were mostly involved in plant development, signaling, ROS detoxification and salt acclimation. However, this study provides new molecular information of several genes to understand the salt stress response. These genes would be useful for the enhancement of salt stress tolerance in plants.

Effect of the Freshwater Discharge on Water Quality Variation and in Haechang Bay, Korea (해창만의 수질환경변동 및 담수방류의 영향평가)

  • Lee, Dae-In;Cho, Hyeon-Seo;Lee, Moon-Ok
    • Journal of Environmental Impact Assessment
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    • v.12 no.5
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    • pp.341-348
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    • 2003
  • Seasonal characteristics of water quality and effect of the freshwater discharge during open the tide embankment in Haechang Bay were evaluated. In the freshwater, where interior of the tide embankment, COD and Chl-a exceeded about 4mg/L and $10mg/m^3$, respectively, independent of season, while in the seawater they showed high values in April and July in contrast to the other period due to input of freshwater and increase of phytoplankton, respectively. The content of seawater inorganic nitrogen maintained a relatively high level at inner part of the bay, whereas high values of inorganic phosphorus content was distributed at all over the bay. The limiting factor for algal growth was nitrogen with respect to the N/P ratio. The compass of influence by the freshwater discharge in April was quite different with water pollutants. As a result of the salinity variation with time, the freshwater extended strongly to offshore from the surface layer without mixing with depth when open the tide embankment, and reached within about one hour at a station which is 3.5km from the tide embankment. To effective water quality management of Haechang Bay, discharge rate and pollutant loads should be controlled.

Water quality management of Jeiu Harbor using material cycle model(II) - Characteristics of water quality in Jeiu harbor and the estimation of pollutant loadings - (물질순환모델을 이용한 제주항의 수질관리(II) - 제주항의 수질 특성과 오염부하량 산정 -)

  • 조은일;강기봉
    • Journal of Environmental Science International
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    • v.12 no.3
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    • pp.299-306
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    • 2003
  • The purpose of this study is to investigate the characteristics of water quality in Jeju harbor and to estimate pollutant loadings discharged into Jeju Harbor. To know characteristics of water quality in Jeju harbor, and pollutant loadings of Sanzi river, we have investigated from August, 2000 to May, 2001. The results showed that the concentrations of COD, DIN and DIP were in the range of 1.00∼4.85 mg/L (mean 2.15 mg/L), 2.14∼74.0 $\mu\textrm{g}$-at/L(mean 12.20 $\mu\textrm{g}$-at/L) and 0.52∼4.00 $\mu\textrm{g}$-at/L(mean 1.18 $\mu\textrm{g}$-at/L), respectively. These values were under III class of seawater quality criteria. The ratio of nitrogen to phosphorus was lower than 16 except for Station 1 in Jeju harbor. Therefore, nitrogen was playing an important role in phytoplankton growth as limiting factor in Jeju harbor. The mean values of eutrophication index were exceeding 1, which was the eutrophication criteria. The results of estimating pollutant loadings at Sanzi river are 0.30 ton/day for COD, 300 kg/day for DIN and 18.0 kg/day for DIP, respectively.

Characteristics of Pollutant Loads and Water Quality in Kwangyang Bay, Korea

  • Lee Dae-In;Park Chung-Kil;Cho Hyeon-Seo
    • Fisheries and Aquatic Sciences
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    • v.6 no.3
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    • pp.149-154
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    • 2003
  • The characteristics of pollutant loads from the various sources and seawater quality in Kwangyang Bay were evaluated. Total flow rate was estimated to be $10,868,066.8 m^3/day$ with a flow rate of R2l (the Seomjin River) as the highest one. Total COD, TN and TP loads of the input rivers and the ditches were about 27,591.8, 25,029.6 and 586.4 kg/day, respectively. Wastewater discharging loads was the greatest contributors to pollutant loads in the inner part of Kwangyang Bay. COD values in the inner part of the bay was over 3.0 mg/L, which exceeded the seawater quality criteria III of Korea. The average values of DIN and DIP were 8.62 ${\mu}gN/L\;and\;1.26\;{\mu}gP/L$, respectively. The limiting factor for algal growth was DIN. In he total discharging loads of the watershed from unit loading estimations, BOD, TN and TP were 9,132.3, 2,727.2 and 304.2 kg/day, respectively. In addition, municipal sewage by the population as pollution sources and the city of Kwangyang as administrative district had the highest loads. For a appropriate water quality recovery of Kwangyang Bay, it is suggested that it is essential to estimate reduction rate of total pollutant loads by water quality modeling.