• 한국환경생태학회지
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• 제30권1호
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• pp.92-97
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• 2016

본 연구는 미세조류 및 해조류 유주자의 피막화가 이들의 생장 및 생존에 미치는 영향을 파악할 목적으로 수행되었다. 갈조류의 세포벽을 구성하는 녹말과 알긴산을 다양한 비율로 혼합하여 친환경 피막소재로 이용하였으며, 염화칼슘수용액에 적하(form a drop)한 후 피막의 형성 여부를 확인하였고, 미세조류 Nannochloropsis salina와 해조류 Ulva australis의 유주자를 피막화하여 생존율 및 성장률을 측정하였다. 미세조류와 해조류 피막은 녹말과 알긴산의 배합비(무게비)가 5:5, 2:8, 0:10일 때에만 형성되었으며, 10:0과 8:2에서는 형성되지 않았다. 형성된 피막 중 녹말과 알긴산의 5:5 배합비에서 미세조류 개체수가 $8.74{\times}10^5cells\;mL^{-1}$로 최대 증가를 보였으나, 알긴산만을 이용한 피막에서는 $4.92{\times}10^5cells\;mL^{-1}$로 가장 낮았다. 따라서, 녹말과 알긴산의 혼합으로 구성된 피막에서 녹말의 함량이 증가할수록 미세조류 및 해조류 유주자의 생존 및 생장율은 증가되지만 알긴산이 50% 이하인 배합비에서는 물성이 약한 것으로 나타났다. 또한, 인공어초 표면에 응용한 결과, 99 개체 $cm^{-2}$가 발아하는 것으로 나타났다. 본 연구는 해양생태계 복원을 위한 해조류 유주자의 생존율 및 생장률을 향상 시킬 수 있는 다양한 기초자료가 될 것으로 사료되어진다.

• Asian-Australasian Journal of Animal Sciences
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• 제29권3호
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• pp.384-389
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• 2016

A total of 400, one day-old commercial broiler chicks were divided into five diet groups (negative control, positive control group with 55 ppm Zn-bacitracin, 15 ppm ${\beta}$-glucan, 30 ppm ${\beta}$-glucan, and 60 ppm ${\beta}$-glucan) and fed for six weeks. Ten broilers were allotted to each of 40 floor pens. Eight floor pens were randomly assigned to one of the 5 diets. Each diet was fed to the broilers for 6 weeks with free access to water and diet. The survival rate, growth rate, feed efficiency, and feed conversion rate of the broilers were calculated. At the end of the feeding trial, the birds were slaughtered, breast muscles deboned, and quality parameters of the breast meat during storage were determined. The high level of dietary ${\beta}$-glucan (60 ppm) showed better feed conversion ratio and survival rate than the negative control. The survival rate of 60 ppm ${\beta}$-glucan-treated group was the same as that of the antibiotic-treated group, which showed the highest survival rate among the treatments. There was no significant difference in carcass yield, water holding capacity, pH, color, and 2-thiobarbituric acid reactive substances values of chicken breast meat among the 5 treatment groups. Supplementation of 60 ppm ${\beta}$-glucan to broiler diet improved the survival rate and feed conversion rate of broilers to the same level as 55 ppm Zn-bacitracin group. The result indicated that use of ${\beta}$-glucan (60 ppm) can be a potential alternative to antibiotics to improve the survival and performance of broilers. However, dietary ${\beta}$-glucan showed no effects on the quality parameters of chicken breast meat.

• 한국양식학회지
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• 제11권2호
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• pp.183-191
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• 1998

조개류의 인공종묘생산시 생멱이생물의 대체먹이원을 개발하기 위해 냉동 EH는 건조한 먹이생물의 먹이효율을 조사하였다. T. suecica를 대상으로 실험구를 생 T. suecica, 건조 T. suecica, 냉동 T. suecica, 생 T. suecica(50%)+냉동 T. suecica(50%), 생 T. suecica(50%)건조 T. suecica(50%)를 구분하여 참굴과 개량조개 유생 그리고 바지락 치패에게 먹이로 공급하며 먹이효율을 비교하였다. 참굴 유생의 경우는 생 T. suecica를 공급한 실험구가 각고 $231.9^{\mu}$m로 성장이 가장 좋았고, 생존율은 72.6%로 가장 높게 나타났으며 다른 실험구와 유의적인 차이를 보였으며 건조 T. suecica를 단독으로 공급한 실험구에 각고 $168.7^{\mu}$m, 생존율 39.3%로 가장 저조한 것으로 나타났다. 개량조개 유생에서는 생 T. suecica를 공급한 실험구가 각장 $213.0^{\mu}$m, 생존율 42.9%로 가장 좋았으나 다른 실험구와 유의적인 차이가 없었다. 바지락 소형 치패의 경우 성장은 생 T. suecica, 생 T. suecica(50%)+냉동T. suecica50%)를 공급한 실험구와 다른 실험구와 유의적인 차이를 보였다. 그리고 대형 치패 경우 성장은 생 T. suecica, 생 T. suecica(50%)+건조 T. suecica(50%) 실험구와 유의적인 차이를 나타냈다. 그러나 바지락 치패의 생존율에서는 유의적인 차이를 보이지 않았다. 이와같이 냉동, 건조된 T. suecica의 먹이효율은 유생의 종류와 성장단계에 따라 다른 것으로 나타났으며 생 T. suecica를 부분적으로 대체할 수 있을 것으로 판단된다.

• BMB Reports
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• 제35권1호
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• pp.106-115
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• 2002

The serine/threonine kinase Akt has been intensely studied for its role in growth factor-mediated cell survival for the past 5 years. On the other hand, the ongoing research effort has recently uncovered novel regulatory mechanisms and downstream effectors of Akt that demonstrate the involvement of Akt in other cellular functions such as cell cycle progression, angiogenesis, and cancer cell invasion/metastasis. Furthermore, recent studies using whole model organisms suggest additional roles for Akt in important diseases such as aging and diabetes. The following review addresses these recent advances in the understanding of Akt function.

• Tuberculosis and Respiratory Diseases
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• 제79권4호
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• pp.248-256
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• 2016

Somatic mutations that lead to hyperactivation of epidermal growth factor receptor (EGFR) signaling are detected in approximately 50% of lung adenocarcinoma in people from the Far East population and tyrosine kinase inhibitors are now the standard first line treatment for advanced disease. They have led to a doubling of progression-free survival and an increase in overall survival by more than 2 years. However, emergence of resistant clones has become the primary cause for treatment failure, and has created a new challenge in the daily management of patients with EGFR mutations. Identification of mechanisms leading to inhibitor resistance has led to new therapeutic modalities, some of which have now been adapted for patients with unsuccessful tyrosine kinase inhibitor treatment. In this review, we describe mechanisms of tyrosine kinase inhibitor resistance and the available strategies to overcoming resistance.

• 한국수산과학회지
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• 제49권4호
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• pp.467-473
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• 2016

Several of our recent studies have focused on mass production of the marine Cladoceran Diaphanosoma celebensis, which can serve as a feed substitute for Artemia nauplii in the culture of larval marine fish. We compared the growth and survival rates of tiger puffer Takifugu rubripes fed D. celebensis with those fed Artemia. The group fed D. celebensis showed an increase in growth from 0.10 g to 1.78 g, while the group fed enriched Artemia had a growth from 0.10 g to 0.92 g. The survival rate of fish fed D. celebensis was 86.7% while that of fish fed enriched Artemia was 51.1%, indicating that the former group was superior to the latter both in growth and survival rate. Fatty acid analysis revealed that D. celebensis fed Nannochloropsis oculata and Schizochytrium sp. had an eicosapentaenoic acid (EPA) content of 16.42% and a docosahexaenoic acid (DHA) content of 3.93%. Meanwhile, juvenile tiger puffer fed D. celebensis had an EPA content of 9.12% and a DHA content of 10.69%. Juvenile tiger puffer fed D. celebensis had a similar n-3 highly unsaturated fatty acid content to that of tiger puffer fed enriched Artemia which had an EPA content of 5.82% and a DHA content of 15.90%, indicating that no additional enrichment was required.

• 한국패류학회지
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• 제28권1호
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• pp.1-6
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• 2012

2002년 6월부터 2003년 10월까지 충남 태안군 안면읍 내파수도 연안의 수하식 양식장에서 비단가리비 치패 (평균 각고 $32.97{\pm}6.21mm$, 전중량 $5.63{\pm}3.86g$) 를 채롱에 수용하여 밀도에 따른 성장과 생존을 연구하였다. 비단가리비는 주로 한국의 서해안과 중국의 북부 연안에 자연적으로 서식하는 종이다. 조사해역의 표층 수온은 $4.3-25.3^{\circ}C$, 염분은 29.2-32.1 psu, 용존산소는 5.32-7.51 mg/L이었고, pH는 7.84-8.12로 나타났다. 실험을 시작할 때 채롱 당 수용밀도는 20, 30, 40, 50 개체로 하였다. 실험을 시작하여 16개월이 경과한 후의 평균 각고는 64.35-76.23 mm였고, 전중량은 41.53-64.85 g, 생존율은 82-100%로 나타났다. 비단가리비의 성장률은 수용밀도와 역상관 관계를 가지고 있었다. 각고와 전 중량의 성장은 수온이 하강하면서 감소하였다. 비단가리비의 폐사는 대부분 3-4월과 9-10월에 나타났다. 밀도에 따른 생존율은 밀도가 증가함에 따라 감소하였으며 20마리 실험구에서 가장 높게 나타났다.

• 구강회복응용과학지
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• 제36권3호
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• pp.145-157
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• 2020

목적: 본 연구에서는 구강연조직재생에서 인간치은섬유아세포에 hydrophobically modified glycol chitosan (HGC) 기반 나노방출제어시스템을 적용 시 PI3K-AKT 신호전달의 세포생존 연관 유전자를 통해 trichloroacetic acid (TCA)와 epidermal growth factor (EGF)의 영향을 확인하고자 하였다. 연구 재료 및 방법: TCA와 EGF를 방출하는 나노방출제어시스템을 제작하였다. 실험군은 3가지 군으로 나누었다; 대조군(CON), TCA-담지형 나노방출제어시스템 투여군(EXP1), TCA-와 EGF- 담지형 나노방출제어시스템 투여군(EXP2). 인간치은섬유아세포 배양 후 PI3K-AKT 신호전달에 연관된 총 29개 유전자를 실시간 중합효소연쇄반응으로 분석했다. 일요인 분산분석 및 다중회귀분석이 사용되었다. 결과: 세포생존 관련 유전자들은 EXP1과 EXP2에서 상향조절되었다. 다중회귀분석에서는 ITGB1이 AKT1의 발현에 가장 영향력 있는 요소로 결정되었다. 결론: HGC기반 나노방출제어시스템을 통한 TCA와 EGF의 적용은 세포생존에 관한 신호전달을 상향 조절시킬 수 있다.

• 한국임상약학회지
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• 제18권2호
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• pp.75-83
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• 2008

Purpose: Currently lung cancer ranks second in cancer for incidence rate and is a disease that ranks first for a death rate by cancerous growth because it is already advanced at the time of diagnosis. The purpose of this paper was to analyze the factors that affect the effectiveness of and rash occurrence by Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitor (EGFR TKI) in patients with non-small cell lung cancer. Methods: A retrospective chart review of 100 patients, who took EGFR TKI (erlotinib, gefitinib) among patients who were diagnosed with non-small cell lung cancer in a Hospital in Korea between May 2005 and February 2008, was conducted. The drug effectiveness was evaluated by Response Evaluation Criteria In Solid Tumor. Results: EGFR mutation was the only factor associated with drug response (complete response and partial response). When stable disease was added to drug response as the evaluation parameter, ECOG and rash as well as EGFR mutation were found to be important factors. Survival, however, was not affected by EGFR mutation. The factors influenced on survival were older age (${\geq}65$), low ECOG ($1{\sim}2$), adenocarcinoma and rash. In the case of rash, group with EGFR mutation or low ECOG showed significantly higher chance of occurrence. There was no significant difference in rash occurrence between gefitinib and erlotinib groups. Conclusions: Based on the results, EGFR mutation positive and low ECOG ($1{\sim}2$) were significantly important factors for both effectiveness of EGFR TKI and rash occurrence. Also, rash itself was found to be an independently significant factor for the disease control and survival. Therefore, while administering EGFR TKI, patients who have the factors associated with rash occurrence should be closely monitored for effective and safe drug therapy.

• 한국수산과학회지
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• 제41권2호
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• pp.125-133
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• 2008

To investigate the effect of repeated food deprivation and refeeding on the hyperphagia, compensatory growth, feed efficiency, body composition, hepatosomatic index (HSI), and survival rate of the juvenile olive flounder Paralichthys olivaceus, an experiment was conducted for 90 days. Feeding treatments consisted of the following five regimes for 90 days from August to October, 2005: C: daily satiation feeding (control), S1: 1-day satiation feeding after 1-day food deprivation, S2: 1-day satiation feeding after 2-day food deprivation, S3: 1-day satiation feeding after 3-day food deprivation and S4: 1-day satiation feeding after 4-day food deprivation, respectively. Although the monthly feed intake (MFI) of the control was significantly higher than that of all of the starved groups, the daily feed intake (DFI) was more higher in S1, S2, and S3 than that in the control as a result of hyperphagia after starvation. While the feed efficiency in the summer (to day 30) decreased in all of the starved groups with prolongation of the starvation period, the feed efficiency in the autumn (to day 90) was increased with prolongation of the starvation period. The whole body proximate composition and HSI were also affected by starvation. The crude protein, lipid, and HSI decreased with prolongation of the starvation period, whereas the crude ash and moisture increased. The growth rate and condition factor also decreased in proportion to the starvation period. The survival rate was highest in the control and was the lowest in S4. In this study, although hyperphagia occurred in the deprived groups, we knew that the compensatory growth did not always occur.