• Title/Summary/Keyword: Growth Inhibition

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Selection of Microorganisms for Probiotics and Their Characterization (생균제로서 가능성이 있는 미생물의 선별 및 특성)

  • 박홍석;이선희;엄태붕
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.27 no.3
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    • pp.433-440
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    • 1998
  • In order to select probiotics with a high survival rate in gut and the growth inhibition of virulent pathogens to human beings or animals, we have examined a variety of microorganisms to assess the acid, bile, and pancreatic tolerance and the growth inhibition of E. colt O8 and Salmonella choleraesuis ATCC 8391. Lactobacillus acidophilus KCTC 3155 was shown to inhibit the growth of E. coli and Salmonella dramatically within 24 h of incubation, although it was vulnerable to the exposure of bile acids. Bacillus polyfermenticus showed a good growth inhibition against E. coli, with a moderate acid and bile tolerance, while Clostridium butyricum KCTC 1786 inhibited the growth of E. coli and Salmonella slightly with a good bile tolerance. However, Saccharomyces cerevisiae KCTC 7928 and Aspergillus oryzae KCTC 6075 did not inhibit the growth of E. coli and Salmonella, suggesting that these microorganisms can be used as the sources of nutritional suppliment rather than as probiotics itself.

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Portulaca oleracea L. 추출물에 의한 Escherichia coli KCTC 2441의 생육억제

  • Lee, Eun-Sook;Seo, Bu-Il
    • Herbal Formula Science
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    • v.9 no.1
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    • pp.367-374
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    • 2001
  • This study was performed to investigate the effects of purslane extracts on growth inhibition of Escherichia coli KCTC 2441. The purslane extract is effective on retarding growth of Escherichia coli KCTC 2441 and effect of its extract was founded from 1% concentration. At 7% the growth of Escherichia coli KCTC 2441 was suddenly retarded. The stem of purslane in a sunny place on June is more effective than its leaf. Leaves with a red violet color has high effect on retarding growth of Escherichia coli KCTC 2441. These results suggest that purslane extract is effective in growth inhibition of Escherichia coli KCTC 2441.

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Growth Inhibition Effect of Environment-friendly Agricultural Materials in Botrytis cinerea In Vitro (친환경 유기농자재의 잿빛곰팡이병 병원균의 생장 억제 효과)

  • Kwak, Young-Ki;Kim, Il-Seop;Cho, Myeong-Cheoul;Lee, Seong-Chan;Kim, Su
    • Journal of Bio-Environment Control
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    • v.21 no.2
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    • pp.134-139
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    • 2012
  • Inhibition effects on spore germination and mycelia growth for gray mold (Botrytis cinerea) were investigated in vitro using environment-friendly agricultural materials as well as environment-friendly pesticides. The inhibition effect on mycelia growth of gray mold is the highest when the gray mold mycelia were treated with a pesticide (commercial name: Koreayeok, Jihabudea KM, Sootingtan, Sootingstar) that contains a mixture of Bacillus subtilis, resulting in 100% inhibition of the mycelia growth. Meanwhile, the range of less than 20% inhibition effects on the growth of gray mold mycelia was observed with other commercial agricultural materials. The significant inhibition effects on spore germination of gray mold fungus were shown in vitro with two water dispersible pesticides containing sulfur [BTB (97.7%) and SulfurStar (92.3%)], respectively. These in vitro results of inhibiting of the spore germination and mycelia growth together cannot found. It remains to be determined whether the selected environment-friendly agricultural materials in effective control of gray mold in vitro can be used to control gray mold in field.

Inhibition of Adventitious Root Growth in Boron-Deficient or Aluminum-Stressed Sunflower Cuttings

  • Hong, Jung-Hee;Go, Eun-Jung;Kim, Tae-Yun
    • Journal of Environmental Science International
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    • v.12 no.11
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    • pp.1189-1196
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    • 2003
  • The effect of boron and aluminum on the development of adventitious roots was studied in sunflower cuttings. Three-day-old seedlings were de-rooted and grown in nutrient solutions with or without boron and supplemented with different concentrations (from 50 to 700 ${\mu}$M) of aluminum. The number and length of the adventitious roots and proline content in adventitious roots in response to insufficient boron and aluminum stress were determined periodically. The micronutrient boron caused the development of numerous roots in the lower parts of the hypocotyl. A dose-response of boron-induced rooting yielded an optimum concentration of 0.1 mM boron. In the absence of boron, in the majority of the adventitious roots, a significant inhibition was observed with or without aluminum, indicating that the most apparent symptom of boron deficiency is the cessation of root growth. Increasing concentrations of aluminum caused progressive inhibition of growth and rooting of the hypocotyls, and a parallel increase in proline levels of adventitious roots. Supplemental boron ameliorated the inhibitory effect of aluminum, suggesting that aluminum could inhibit root growth by inducing boron deficiency. Ascorbate added to medium in the absence of boron improved root growth and induced a significant decrease in proline levels. These findings suggest that adventitious root growth inhibition resulting from either boron deficiency or aluminum toxicity may be a result of impaired ascorbate metabolism.

Studies on the effect of Sophora flavescens extract on the hair growth stimulation and acne inhibition (苦蔘抽出物이 毛髮成長 促進 및 面疱 抑制에 미치는 영향)

  • Roh, Hyun-Chan;Roh, Seok-Sun
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.15 no.1
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    • pp.96-126
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    • 2002
  • In the course of screening natural extracts for hair growth, we found that the extract of dried root of Sophora flavescens has the prominent hair growth promoting effect. After topical application of Sophora flavescens extract to the back of C57BL/6 mice, the earlier conversion of telogen-to-anagen phase was induced. In addition, the Sophora flavescens extract revealed to possess potent inhibitory effect on $5{\alpha}$-reductase Ⅰ and Ⅱ activity. The growth of dermal papilla cells and mouse vibrissae hair follicle cultured in vitro, however, was not affected by Sophora flavescens extract treatment. RT-PCR analysis showed that Sophora flavescens extract induced mRNA levels of growth factors such as insulin-like growth factor-Ⅰ and keratinocyte growth factor in dermal papilla cells, suggesting hair growth promoting effect of Sophora flavescens extract is mediated through inhibition of $5{\alpha}$-reductase type Ⅱ activity and the regulation of growth factors in dermal papilla cells. Furthermore, Sophora flavescens extract also showed anti-bacterial effect on Propionibacterium acnes. These results suggest that Sophora flavescens can be used as a potent treatment agent for helping hair growth stimulation and acne inhibition.

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Influence of Selected Turfgrass Herbicides, Simazine, Pendimethalin, Propyzamide and Asulam on the Growth of Zoysia matrella (Zoysia matrella의 생장에 미치는 잔디용 제초제 Simazine, Pendimethalin, Propyzamide, Asulam의 영향)

  • 김석정
    • Asian Journal of Turfgrass Science
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    • v.7 no.2_3
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    • pp.81-94
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    • 1993
  • This study was conducted to investigate the influence of herbicides, simazine, pendimethalin, propyzamide and asulam on the growth of Zoysia matrella when they are treated at different growth stages of the plant. The reaction of Zoysia matrella to herbicides differed depending on kinds of herbicides, their concentcations, and time of treatment. Simazine at the higher concentration of 600g /l0a exhibited inhibitory effect on leaf dry matter and growth of runner when treated on April 5, 1991. The highest inhibition of leaf and runner was observed in the plot applied on May 23, 1990. The sprouting and growth of Zoysia matrella was not affected by all the concentrations of the herbicides applied on October 23,1991. Pendimethalin did not influence the growth of Zoysia matrella at he treatment of April 5. However, at the treatment of May 23, the number and growth of runner was inhibited although the plant height and leaf dry matter was not affected. When pendimethalin was treated on October 23, the growth of runner was very slightly reduced in the next year. In case of propyzamide, there was no inhibitory effect on the growth of Zoysia matrella regardless of treatment of times, methods, and dosages. Greater inhibition in the growth of Zoysia matrella was observed at higher dosage(1,ll0g /l0a)-treated plot on April 5 and all the concentrations treated on May 23. But, no inhibition was observed on the October 23 treatment.

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Growth and Chlorophyll Biosynthesis of Vigna angularis under Lead Stress

  • Suh-Young Koo;Sun
    • Journal of Environmental Science International
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    • v.1 no.2
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    • pp.145-155
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    • 1992
  • The effect of various supplies of lead singly and in combination with aluminium on growth and chlorophyll biosynthesis was investigated in 7-day-old Vigna anguluris seedlings. Expose to 50 $\mu$N Pb or more drastically reduced root elongation rate. Significant depressions in root growth was observed within 1 day and no recovery of growth was seen over the duration of treatment period. Root elongation decreased depending on the Pb concentrations. Root growth inhibition was stronger than shoot growth inhibition. The initiation of lateral roots appeared to be more sensitive to Pb than the growth of main roots. Inhibition of root and shoot elongation by Pb was lessened by combined exposure of Pb and Al, suggesting that the presence of AA reverse the inhibitory effect of Pb alone. With the histochemical sodium rhodizonate method the rate of Pb uptake was dependent on the Pb concentration and exposure time of the roots to Pb salts. Pb was first deposited on the root surface and then translocated radially in the root cap cells. During a longer Pb administration (up to 72 h) Pb penetration was nonuniform, with accumulation within the cortex or endodermis. There was drastic reduction in chlorophyll content by Pb. The Pb inhibition of chlorophyll synthesis was concentration dependent. 5-Aminolevulinic acid dehydratase (ALAD) activity exhibited distinct inhibition from control. Reduction in chlorophyll content was accompanied by proportional changes in ALAD activity. Chlorophyll content and ALAD activity were less affected by combined exposure of Pb and Al, suggesting that Al has a protective effect against the inhibiting action of Pb on photosynthetic activity.

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Growth and Chlorophyiil Biosynthesis of Vigna angularis under Lead Stress

  • Koo Suh-Young;Jin Sun-Young;Hong Jung-Hee
    • Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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    • v.1 no.2
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    • pp.145-155
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    • 1997
  • The effect of various supplies of lead singly and in combination with aluminium on growth and chlorophyll biosynthesis was investigated in 7-day-old Vigna angularis seedlings. Expose to 50 uM Pb or more drastically reduced root elongation rate. Significant depressions in root growth was observed within 1 day and no recovery of growth was seen over the duration of treatment period. Root elongation decreased depending on the Pb concentrations. Root growth inhibition was stronger than shoot growth inhibition. The initiation of lateral roots appeared to be more sensitive to Pb than the growth of main roots. Inhibition of root and shoot elongation by Pb was lessened by combined exposure of Pb and Al, suggesting that the presence of Al reverse the inhibitory effect of Pb alone. With the histochemical sodium rhodizonate method the rate of Pb uptake was dependent on the Pb concentration and exposure time of the roots to Pb salts. Pb was first deposited on the root surface and then translocated radially in the root cap cells. During a longer Pb administration (up to 72 h) Pb penetration was nonuniform, with accumulation within the cortex or endodermis. There was drastic reduction in chlorophyll content by Pb. The Pb inhibition of chlorophyll synthesis was concentration dependent. $\delta-Aminolevulinic$ acid dehydratase (ALAD) activity exhibited distinct inhibition from control. Reduction in chlorophyll content was accompanied by proportional changes in ALAD activity. Chlorophyll content and ALAD activity were less affected by combined exposure of Pb and Al, suggesting that Al has a protective effect against the inhibiting action of Pb on photosynthetic activity.

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