• Journal of mucopolysaccharidosis and rare diseases
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• 제1권2호
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• pp.49-53
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• 2015

Prader-Willi syndrome (PWS) is a complex multisystem genetic disorder characterized by hypothalamic-pituitary dysfunction. Many features of PWS indicate a deficiency in growth hormone (GH) production, and these findings provide a rationale for GH therapy in PWS. It is possible that rhGH therapy could have beneficial effects in adults with PWS, similar to those in adults with GH deficiency (GHD) of non-syndromic cause. However, there is a paucity of data on the use of GH in adults with PWS. Here, the previous studies about efficacy and safety of rhGH therapy in PWS adults are summarized. Briefly, rhGH therapy in PWS adults may improve body composition, leading to increased lean body mass and decreased fat mass, as well as decreased subcutaneous and visceral adiposity without overall changes in body mass index. There may be at least transient deterioration in glucose homoeostasis in some PWS patients on rhGH therapy, which requires further study. In addition, clinical care guidelines for rhGH therapy in adults with PWS were suggested.

• 한국가축번식학회지
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• 제16권3호
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• pp.225-230
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• 1992

Many transgenic mice expressing human growth hormone gene were infertile. To investigate the infertility of these transfenic mice, it was looked into the estrus cycle and sexual behaviour and also tested through in vitro fertilization whether the germ cells of these mice normal or not. The infertile female transgenic mice were mated to the fertile males of ICR strain, but in almost all of them the vaginal plugs were not detected and their estrus cycles by vaginal smear were almost irregular which kept up estrus or diestrus stage. Many male transgenic mice did not have the ability of sexual behaviour. Therefore the viability of germ cells in infertile male transgenic mice was investigated by in vitro fertilization, but the sperm were normally fertilized with the eggs and the transgene of parent was passed on to the progeny. These results consequently suggest that the infertility of transgenic mice experssing human growth hormone gene may be due to the physiological activity of human growth hormone, not germ cells.

• Toxicological Research
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• 제9권1호
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• pp.125-132
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• 1993

Antigenic potential of recombinant human growth hormone (LBD-007), a newly developed drug for growth hormone deficiency, was investigated in mice and guinea pigs. 1. Mice showed production of antibodies against LBD-009 (1.5IU/kg) with aluminum hydroxide gel(alum) as an adjuvant, Judaged by the heterologous anaphylaxis (PCA) test using rats. On the other hand, antibodies against ovalbumin (OVA) inoculated with alum were definitely detected. 2. In the studies with guinea pigs, both the inoculation of LBD-009 (0.15IU/kg-1.5IU/kg) only and of LBD-009 with complete Freund's adjuvant (CFA) as an adjuvant did produce weak positive reactions in homologous passive cutaneous anaphylaxis (PCA). On the other hand, the inoculation of ovalbumin with complete Freund's adjuvant (CFA) produced positive reaction in PCA.

• Journal of mucopolysaccharidosis and rare diseases
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• 제2권2호
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• pp.41-42
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• 2016

Today's mobile phones and tablet PCs offer a considerably wider range of functionalities than before. Mobile applications (apps) are increasingly used for managing various daily health tasks. Currently, more than 165,000 health-related apps are offered on all the stores of different platforms. Pf Jin and the Association for Research on MPS and Rare Diseases (AMARD) have helped Prader-Willi syndrome (PWS) families through medical information and family support since 2015. AMARD developed the first mobile application for Korean patients with PWS, which was released to a limited number of patients under the age of 3 and only provided to Android users. The first version of the PWS application focused on growth hormone therapy and the assessment of growth and development by parents in infant and early-childhood PWS patients. The 2016 version of the PWS application has been improved in many different ways. We have expanded the subjects of the application to late childhood and adolescent groups, changed the user interface accordingly, and made the application available for iOS users. We will show the specialized growth curves of older children with PWS. Therefore, patients with PWS over the age of 3 and their parents can assess the patients' growth. Additionally, we have upgraded the growth hormone therapy menu by improving the input system for the growth hormone therapy injection schedule and the daily growth profile (height and weight). We expect that the new version of the PWS application will help many PWS families cope with growth hormone therapy and evaluate the effects of growth hormones in better ways. Additionally, we are making a constant effort to provide more useful information about patients with PWS in many aspects.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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• pp.127.2-128
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• 2002

• 대한한방소아과학회지
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• 제12권1호
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• pp.277-287
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• 1998

The present study is designed that the herb medicines which are returned to kidney, pancreas and are known by efficacy in growth are supplied to pigs, thereafter the effects to growth and secretion of growth hormone in serum is investigated. For this purpose the 24 days old pigs, which are crossbreeding, castrated are used. The conclusions are that after 4 weeks' supply of herb medicinal compounds, pigs' weight gain has increased in low nutritional group with statistical meaning(p〈0.05). And one day's weight gain has increased with statistical meaning(p〈0.05) in all groups. Regarding food take, the second nutritional group has more significant increase than control group. Utilization rate of nutrition doesn't have any statistical meaning in all groups, but it has the tendency that as herb medicinal compounds are added, utilization rate of nutrition is increased. Increase of vertebra lengths and level of growth hormone in serum according to supply of herb medicinal compound don't have any statistical meaning because of small sample size, but 10 - 20 % increase of vertebra lengths and 10 - 30 % increase of level of growth hormone are observed.

• 한국발생생물학회지:발생과생식
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• 제4권2호
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• pp.237-242
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• 2000

흰쥐 시상하부에서 합성ㆍ분비되어 뇌하수체 전엽에서의 growth hormone (GH) 분비를 촉진하는 growth hormone releasing hormone (GHRH)이 시상하부 이외 조직들 (extrahypothalamic tissues)인 태반, 생식소, 그리고 뇌하수체 전엽에서도 발현됨이 보고되었다. 본 연구는 흰쥐 뇌하수체 전엽에서 발현되는 GHRH의 기능을 조사하기 위해 i)세포 배양을 시행하면서 GHRH의 세포내 함량, 분비 그리고 세포분획법 (cell-fractionation)을 사용하여 분리한 뇌하수체 세포 유형별로 GHRH 함량을 방사면역측정법으로 조사하였고, ii)체외배양 중인 뇌하수체 전엽세포의 증식에 미치는 GHRH의 효과를 측정하기 위해 [$^3$H] thymidine incorporation assay를, 그리고 iii) GHRH의 세포분열 촉진 효과와 세포내 c-fos 유전자 발현과의 상관관계를 조사하기 위해 northern blot analysis를 시행하였다. GHRH 방사면역측정법을 시행한 결과 상당량의 GHRH-like 분자들이 흰쥐 뇌하수체 전엽내에 존재하고, 체외 세포배양시 분비됨을 관찰하였다. 세포분획을 사용한 실험에서 GHRH 함량은 gonadotrope, somatotrope, lactotrope 그리고 thyrotrope 순으로 나타났다. 이 러한 결과는 흰쥐 뇌하수체 전엽에서 생성된 GHRH가 국부적인 조절인자, 특히 상이한 유형의 세포들 간의 상호조절 (cross-talk)을 통해 뇌하수체 전엽에서의 세포분열과 분화, 그리고 기능조절에 관여할 가능성을 보여주었다. GHRH는 체외 배양중인 뇌하수체 전엽세포의 [$^3$H] thymidine incorporation을 농도의존적으로 증가시켰으며, 이러한 GHRH의 세포분열 촉진 효과는 예상대로 세포내 oncogene 활성 의 증가를 통해 일어나는 것임을 c-fos northrn blot으로 확인하였다. 결론적으로, 본 연구는 흰쥐 뇌하수체 전엽에서 합성되는 GHRH가 paracrine 또는 autocrine 기작으로 GH의 분비 촉진 이외에도 세포분열의 조절함을 시사하는 것이다.

• Asian-Australasian Journal of Animal Sciences
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• 제20권7호
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• pp.1030-1038
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• 2007

Results on localization of growth hormone receptor (GHR), interaction of growth hormone (GH) with receptor in buffalo adipose tissue and identification of activated signaling molecules in the action of GH are presented. Bovine GH (bGH) was labeled with fluorescein or biotin. Fluorescein-labelled bGH was used for localization of GHRs in buffalo adipocytes. The receptors were present on the cell surface. The affinity of binding of GH to its receptor was determined by designing an experiment in which buffalo adipose tissue explants, biotinylated GH and streptavidin-peroxidase conjugate were employed. The affinity constant was calculated to be $2{\times}10^8M^{-1}$. The receptor density on adipose tissue was found to be 1 femto mole per mg of tissue. Signalling molecules generated in the action of GH were tentatively identified by employing Western blot and enhanced chemiluminescence techniques using anti-phosphotyrosine antibody. Based on molecular weights of proteins reactive to anti-phosphotyrosine antibody, three signaling molecules viz. insulin receptor substrate, Janus activated kinase (Jak) and mitogen activated protein were tentatively identified. These signaling molecules appeared in a time (incubation time of explants with growth hormone) dependent way. The activation of Jak2 was confirmed by employing anti-Jak2 antibody in a Western blot. The activation of Jak2 occurred during 5 min incubation of buffalo adipose tissue explants with GH and incubation for an additional period, viz. 30 min. or 60 min., resulted in a drastic reduction in activation. The results suggest that Jak2 activation is an early event in the action of GH in buffalo adipose tissue.

• 한국발생생물학회지:발생과생식
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• 제6권1호
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• pp.31-35
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• 2002

생쥐에서 페르몬으로 알려진 소수성의 분자와 결합하는 major urinary proteins(MUPs)는 부분적으로 성장 호르몬(GH)의 조절을 받는다. 본 연구에서는 MT-GRF로 형질전환되어 성장호르몬이 증가된 생쥐에서 MUP의 발현을 조사하였다. 이 MT-GRF로 형질전환된 생쥐의 소변에서 MUP이 대조군보다 심하게 저하되어 나타났고, 암컷보다 수컷에서 더 저하되어 나타났다. 또한 MT-GRF를 근육에 주사한 생쥐에서도 MUP는 저하되어 나타났다. 부가하여, MT-GRF로 형질전환시킨 생쥐의 소변에서 전기영동상에서 albumin과 동일하게 이동하는 고분자의 단백질이 다량으로 관찰되었는데 신장의 사구체 여과가 손상되었음을 암시하고 있다. 이 혈장 단백질의 손실도 암컷보다 수컷에서 심하게 나타났다. 결과적으로 GRF의 과다생성은 성별에 차별화된 영향을 미치면서 GH로 형질전환된 동물모델에서 관찰되는 MUP의 발현과 사구체 기능의 변화를 동일하게 유발하고 있었다.

• 대한통합의학회지
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• 제6권1호
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• pp.63-74
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• 2018

Purpose: The purpose of this study was to evaluate the effect of a trunk stabilization exercise program on the participants' scoliosis angle, pulmonary function, and growth hormones. Method: In the study, 30 participants were divided into a normal scoliosis exercise group (n=15) and an obese scoliosis exercise group (n=15). The participants performed a trunk stabilization exercise program three times a week for 12 weeks, and the exercise sessions lasted 50 minutes. The participants' pulmonary function [FVC, FEV1, FEV1/FVC, and PEF] was measured using a CardioTouch 3000S, and their scoliosis angles were measured using the Cobb's angle. The levels of growth hormone (GH) and insulin-like growth factor-1 (IGF-1) were analyzed on an immunoradiometric assay (IRMA) and radioimmunoassay (RIA), respectively. Results: After the intervention, the scoliosis angle, hormone levels, and pulmonary function increased significantly in both groups (p<.05). The result of the intergroup difference test indicated statistically significant differences in the three items (scoliosis angle, hormone levels, and pulmonary function) between the two groups (p<.05). Conclusion: Therefore, this program may be recommended as a therapeutic intervention for patients with scoliosis.