• KOREAN JOURNAL OF CROP SCIENCE
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• v.64 no.1
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• pp.55-62
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• 2019

Rapeseed meal, which is a byproduct of rapeseed oil extraction, improves crop productivity by supplying nutrients to the soil. The present study aimed to manufacture fermented rapeseed meal compost using two effective microbial agents and evaluate their efficiency as fertilizer. To types of fermented rapeseed meal, manufactured using either a bio-carrier or microbial agent, showed no differences in pH, electrical conductivity (EC), and total nitrogen content. However, the contents of $NH_4-N$ and $NO_3-N$ as inorganic nitrogen were increased by 5.6 times and 1.5 times, respectively, after 5 d of fermentation. Rapeseed meal fermented for 5 d was applied to tomato a basal fertilizer and after eight weeks, the plant height increased in all fermented rapeseed treatments compared to that in the chemical fertilizer treatment, and also the quantum yield of photosystem II (PS II) showed the same trend. The total nitrogen content of tomato leaves treated with a microbial fermented rapeseed meal was twice as high as that of that treated with a chemical fertilizer. It was confirmed that the increase in the tomato height was an effect of the rapeseed meal containing inorganic nitrogen, which can easily be absorbed by plants. From these results, it is considered that fermented rapeseed meal manufactured with an effective microbial agent for 5 d showed the highest inorganic nutrient content and greatest growth enhancement in tomato.

• Journal of The Korean Society of Grassland and Forage Science
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• v.39 no.1
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• pp.45-52
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• 2019

This experiment was carried out to study the change of productivity and feed value in different types of hilly pasture. The pasture utilized in the experiment was placed on the experimental farm of Pyeongchang Campus of Seoul National University. Forage production type(FP; Orchardgrass 18 + Tall Fescue 12 + Timothy 5 + White clover 5 kg/ha) and Public farm type(PF: Orchardgrass 12 + Tall Fescue 18 + Timothy 5 + White clover 5 kg/ha) pasture were established in September 3, 2014 and utilized (cutting or grazing) four times every year. Growth characteristics, yield and forage quality were investigated for two years. Plant height of grasses was the highest in the $1^{st}$ cutting and legumes was in the $2^{nd}$ cutting. Dry matter (DM) content was highest at every the $1^{st}$ cut grasses significantly lower at the $2^{nd}$ harvest (p<0.05). However, there was no significant difference in the $2^{nd}$, $3^{rd}$ and $4^{th}$ harvest in 2016. In the botanical composition change, the portion of legume was gradually increased after pasture establishment and the ratio of weed and bare land was higher at $2^{nd}$ and $3^{rd}$ cutting, but it was decreased at $4^{th}$ harvest. There was no significant difference in 2016 of fresh yield between two farm types (p>0.05). The yield of dry matter showed similar trend of fresh yield and forage production type was higher than that of public farm type (p<0.05). The forage intake by livestock was 1,452 kg/ha in 2015 and 1,743 kg/ha in 2016. Pasture utilization ratio of public farm type pasture was highest in the $3^{rd}$ grazing time. Forage quality of pasture in relation to management type had not significant difference, but there was difference in harvest times. Crude protein (CP) was the lowest in the $1^{st}$ harvest and total digestible nutrient (TDN) was highest in the $1^{st}$ harvest and lowest in the $4^{th}$ harvest. Based on the above results, it is found that the establishment of pasture suitable for farm's situation is important for set up of Korean model of hilly pasture. Although the forage production type is superior on forage productivity, it is recommended that the results will be provided as basic data for management of public farm type in the future.

• Journal of the Korea Organic Resources Recycling Association
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• v.27 no.1
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• pp.49-56
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• 2019

This experiment was conducted to select an optimum application rate of blended biochar pellet as slow release fertilizer during cabbage cultivation. The blended biochar pellet made with a combination(4:6) of biochar and pig manure compost with unloading N, P, K solutions for adjusting about 9% of total nitrogen(TN). The treatments were consisted of the control as recommended application rates for cabbage cultivation in National Institute of Agricultural Sciences, N 40%, N 40% and 0.07M MgO and N 60 % of the blended biochar pellet, respectively, based on nitrogen application of recommended rates to cabbage cultivation. Changes of $NH_4-N$, $NO_3-N$, $P_2O_5$ and $K_2O$ concentrations in the soil and growth characteristic and yield components were investigated and observed during the cabbage cultivation. The experimental result shown that contents of $NH_4-N$, $NO_3-N$ and $K_2O$ of soil in the N 40% were significantly difference(p<0.01) with the control. $P_2O_5$ concentrations of soil in the N 40% were highest among the treatments. The fresh weight per cabbage in the N 40% was not significantly different(p>0.05) from the control, but in the N 40% and 0.07M MgO and N 60% was lower than that of the control. It was considered that an optimum blended biochar application rate for cabbage cultivation was 40% of recommended nitrogen application.

• Journal of Plant Biotechnology
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• v.45 no.4
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• pp.382-391
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• 2018

The objective of this study was to determine the most effective medium condition of shoot proliferation and root formation for the efficient in vitro micropropagation of M.26 (Malus pumila Mill). Simple sequence repeat (SSR) markers were used to analyze the genetic diversity of micro-propagated and greenhouse grown M.26. Shoot proliferation was carried out in MS (Murashige and Skoog) containing benzyladenin (BA, $0.5{\sim}5.0mg{\cdot}L^{-1}$) and thidiazuron (TDZ, $0.01{\sim}0.1mg{\cdot}L^{-1}$). The highest number of shoots (10.67 shoots per explant) was induced by adding BA at a concentration $1.0mg{\cdot}L^{-1}$. TDZ treatments caused higher hyperhydricity rate in cultured explants than in BA treatments. There was no significant effect of both BA and auxin on shoot proliferation, and the optimum proliferation medium for M.26 was MS medium containing $1.0mg{\cdot}L^{-1}$ BA. To find a suitable medium composition for shoot rooting, we tested different concentrations indole-3-butyric acid (IBA) and ${\alpha}$-naphthaleneacetic acid ($0.5{\sim}5.0mg{\cdot}L^{-1}$), MS medium (1/4-1), sucrose ($0{\sim}30g{\cdot}L^{-1}$). The shoots showed good rooting on half-strength MS medium containing $1.0mg{\cdot}L^{-1}$ IBA and $15-20g{\cdot}L^{-1}$ sucrose. The rooting rate (100%), number of roots (10.45 ~ 13.60 roots per explant), root length (7.41 ~ 8.33 cm), and shoot length (4.93 ~ 5.38 cm) were good on this medium. Fifteen SSR primers were detected in a total of 30 alleles in 20 micro-propagated plantlets, all SSR profiles from micro-propagated plantlets were monomorphic and similar to greenhouse grown control plantlet M.26 plant. The results indicated that M.26 micro-propagated plantlets were genetically stable.

• Korean Journal of Plant Resources
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• v.32 no.1
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• pp.53-62
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• 2019

The purpose of this study was to establish the in vitro propagation system by shoot tip culture of six Hosta species native to Korea (Hosta capitata (Koidz.) Nakai, H. clausa Nakai, H. jonesii M.G.Chung, H. minor (Baker) Nakai, H. venusta F.Maek., and H. yingeri S.B.Jones) for mass proliferation and a new cultivar development. The shoot tips of each Hosta species were cultured on MS medium containing eight combinations of 0.5, 1.0, 2.0, 4.0 mg/L BA with 0.1 mg/L NAA, 0.1, 0.5, 1.0, 2.0 mg/L TDZ with 0.1 mg/L NAA, and without any PGRs (control). They were investigated on callus, somatic embryo, crown bud, differentiation and growth of shoot and root, total fresh weight after 8 weeks of culture. In all six Hosta species, callus and somatic embryo induction rate and multiple shooting rate of the PGRs treatment group were higher than that of the control group. The highest number of differentiated shoots were obtained on medium supplemented with 2.0 ㎎/L TDZ in H. capitata (5.4), 1.0 mg/L TDZ in H. clausa and H. jonesii (3.3 and 5.8, respectively), 0.5 mg/L BA in H. minor (11.1), 1.0 mg/L BA and 0.1 mg/L TDZ in H. venusta (8.1), and 0.5 mg/L TDZ in H. yingeri (9.8). In somatic embryo formation, the PGRs treatment group of H. jonesii and H. yingeri were more effective than the control group, and the effects were relatively less in H. capitata, H. clausa Nakai, H. minor, H. venusta. Crown bud formation of four Hosta species (H.capitata, H. clausa, H. jonesiig, and H. yingeri) were also higher in the PGRs treatment group than in the control group. Crown bud formation of four Hosta species (H.capitata, H. clausa, H. jonesiig, and H. yingeri) were also higher in the PGRs treatment group than in the control group. H. clausa showed no significant effect on callus and shoot differentiation regardless of the type and concentration of cytokinin, but slightly increased in formation of crown bud in TDZ.

• Korean Journal of Environmental Agriculture
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• v.37 no.4
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• pp.235-242
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• 2018

BACKGROUND: About 81% of nitrous oxide ($N_2O$) emissions from agricultural land to the atmosphere is due to nitrogen (N) fertilizer application. Mitigation of $N_2O$ emissions can be more effective in controlling biochemical processes such as nitrification and denitrification in the soil rather than decreasing fertilizer application. The use of urease inhibitors is an effective way to improve N fertilizer efficiency and reduce $N_2O$ emissions. Several compounds act as urease inhibitors, but N-(n-butyl) thiophosphoric triamide (NBPT) has been used worldwide. METHODS AND RESULTS: Hot pepper and chinese cabbage were cultivated in five treatments: standard fertilizer of nitrogen-phosphorus-potassium(N-P-K, $N-P_2O_5-K_2O$: 22.5-11.2-14.9 kg/ha for hot pepper and $N-P_2O_5-K_2O$: 32.0-7.8-19.8 kg/ha for chinese cabbage), no fertilizer, and NBPT-treated fertilizer of 0.5, 1.0, and 2.0 times of nitrogen basal application rate of the standard fertilizer, respectively in Gyeonggi-do Hwaseong-si for 2 years(2015-2016). According to application of NBPT-treated fertilizer in hot pepper and chinese cabbage, $N_2O$ emission decreased by 19-20% compared to that of the standard fertilizer plot. CONCLUSION: NBPT-treated fertilizer proved that $N_2O$ emissions decreased statistically significant in the same growth conditions as the standard fertilization in the hot pepper and chinese cabbage cultivated fields. It means that NBPT-treated fertilizer can be applied for N fertilizer efficiency and $N_2O$ emissions reduction.

• Journal of The Korean Society of Grassland and Forage Science
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• v.38 no.4
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• pp.253-259
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• 2018

A new barley (Hordeum vulgare L.) cultivar 'Mihan' having ruminant-palatable semi-smooth awn and good silage quality was developed at National Institute of Crop Science(NICS), RDA in 2016. This cultivar was derived from a cross of the 'SB00T2064' and 'Suwon385' in 2001. And its promising line showed high yielding through the preliminary yield trials(PYT) and advanced yield trials (AYT) in Iksan from 2012 to 2013. It was designated as the 'Iksan487'. 'Iksan487' was conducted to regional yield trials (RYT) in one upland field and five paddy fields around Korea for three years from 2014 to 2016. And it was released as the name of 'Mihan'. It has growth habit of IV, erect plant type, green leaf and semi-smooth awn. Its heading date was April 23 in the paddy field and maturing date was May 24. Plant height of 'Mihan' was 96cm. Mihan's spikes per $m^2$ was 665. It has better winter hardiness and resistance to BaYMV (Barley Yellow Mosaic Virus) than that of barley cultivar 'Youngyang'. The average dry matter of 'Mihan' was about $10.9ton\;ha^{-1}$ in paddy field. And average feed quality of 'Mihan' was 10.3% of crude protein content, 26.1% of ADF (Acid Detergent Fiber), 46.9 % of NDF (Neutral Detergent Fiber) and 68.2% of TDN (Total Digestible Nutrients). 'Mihan' had grade I of silage quality. This cultivar would be suitable for the area above the daily minimum average temperature of $-8^{\circ}C$ in January in Korean peninsula.

• Journal of Life Science
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• v.28 no.11
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• pp.1339-1346
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• 2018

In Korea, edible mushrooms are produced largely on commercial artificial media, so the annual production of spent mushroom substrate (SMS), as a by-product of the mushroom industry, is estimated at over 200 million tons. This SMS is assumed to contain abundant fungal mycelia and pre-fruiting bodies, as well as various nutritive and bioactive compounds that are presently discarded. This study examined the physico-chemical, nutritional, and enzymatic characteristics of uninoculated sterilized medium (USM) and SMS of shiitake mushrooms with the aim of developing a high-value added product from SMS. The contents of crude protein, crude lipid, and ash were higher after the third SMS harvest ($SMS-A-3^{rd}$) than in USM or $SMS-A-1^{st}$. The contents of Ca, Mg, and P in $SMS-A-3^{rd}$ were 2.95, 2.35, and 2.1-fold higher compared than in USM. No As or Cd was detected in USM or SMS. The pH, Brix, and acidity were 4.6, 20.0, and 1.4, respectively in $SMS-A-3^{rd}$, but 5.6, 6.0, and 0.0, respectively, in USM. These results suggest a highly active production of soluble components and organic acids in $SMS-A-3^{rd}$. The distinct color differences noted for USM, $SMS-A-1^{st}$, and $SMS-A-3^{rd}$ could be used as a mycelial growth indicator. Enzyme activity assays using the APIZYM system showed that SMS is a potent source of hydrolysis-related enzymes, especially esterase (C4) and ${\beta}$-glucuronidase. Our results suggested that the SMS of shiitake has a high potential for use in environmental, agricultural, and stock-breeding industries, for example, as active ingredients for sewage treatment, waste-polymer degradation, and feed additives.

• Journal of Food Hygiene and Safety
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• v.34 no.1
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• pp.94-99
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• 2019

The objective of this study is to establish the shelf life of non-pasteurized whole egg, egg yolk and egg white liquid. Each sample was stored for two weeks at $5^{\circ}C$, $10^{\circ}C$, $15^{\circ}C$, and $25^{\circ}C$, and then sensory, microbial, and physicochemical tests were performed periodically. The estimation of shelf life was based on the microbial standards of total viable counts and coliforms. The chemical properties highly correlated with the sensory evaluation were also used. Our results showed that the shelf life was the most influenced by microbial properties. Exceptionally, however, whole egg and white liquid stored at $5^{\circ}C$ and $10^{\circ}C$ with limited bacterial growth were affected by chemical property. The shelf life of the three non-pasteurized liquids was calculated to be less than one day at over $15^{\circ}C$. At $5^{\circ}C$ and $10^{\circ}C$, the shelf life was calculated to be 5 d and 1 d for egg yolk liquid, 5 d and 5 d for egg white, and 7 d and 5 d for whole egg, respectively. Therefore, it is advisable to establish reasonable shelf life in the more specific manner based on consideration of these findings.

• Journal of Environmental Impact Assessment
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• v.27 no.6
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• pp.548-561
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• 2018

The objectives of this study were to construct a three-dimensional (3D) hydrodynamic and water quality model (EFDC) for the river reach between the Daecheong dam and the Sejong weir, which are directly affected by Gap and Miho streams located in the middle of the Geum River, and to evaluate the trophic status and water quality improvement effect according to the flow control and pollutant load reduction scenarios. The EFDC model was calibrated with the field data including waterlevel, temperature and water quality collected from September, 2012 to April, 2013. The model showed a good agreement with the field data and adequately replicated the spatial and temporal variations of water surface elevation, temperature and water quality. Especially, it was confirmed that spatial distributions of nutrients and algae biomass have wide variation of transverse direction. Also, from the analysis of algal growth limiting factor, it was found that phosphorous loadings from Gap and Miho streams to Sejong weir induce eutrophication and algal bloom. The scenario of pollutant load reduction from Gap and Miho streams showed a significant effect on the improvement of water quality; 4.7~18.2% for Chl-a, 5.4~21.9% for TP at Cheongwon-1 site, and 4.2~ 17.3% for Chl-a and 4.7~19.4% for TP at Yeongi site. In addition, the eutrophication index value, identifying the tropic status of the river, was improved. Meanwhile, flow control of Daecheong Dam and Sejong weir showed little effect on the improvement of water quality; 1.5~2.4% for Chl-a, 2.5~ 3.8% for TP at Cheongwon-1 site, and 1.2~2.1% for Chl-a and 0.9~1.5% for TP at Yeongi site. Therefore, improvement of the water quality in Gap and Miho streams is essential and a prerequirement to meet the target water quality level of the study area.