• Archives of Plastic Surgery
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• 제35권4호
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• pp.360-366
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• 2008

Purpose: Botulinum toxin type A(BoTA) can block the release of vasoconstriction cotransmitters as well as acetylcholine in nerve terminal. The authors observed that BoTA increases flap survival by preventing sympathetic collapse of peripheral vessels. Methods: 10 Sprague Dawley rats were divided into control(n=5), and BoTA group(n=5). $3{\times}10cm$ sized random pattern cutaneous flaps were elevated on the dorsal side in both groups. In BoTA group, BoTA was injected into the flap via intradermal to subdermal route, 7 days before the flap elevation. Flap survival rates (survival area/total area) were measured 7 days after the elevation. Cutaneous blood flow was measured in proximal, middle and distal compartments of the flap using laser Doppler flowmetry initially, preoperatively, at immediate postoperation, and 7 days after flap elevation, respectively. Histological examination was performed 7 days after the flap elevation. The number and shape of the vessels were evaluated under microscope. Results: Mean flap survival was $53.18{\pm}6.58%$ in control group and $93.79{\pm}6.06%$ in BoTA group, displaying statistically significant difference(p=0.0008, p<0.05). In the control group, blood flow to the middle and distal compartments of the flap decreased significantly immediately after flap elevation. In the BoTA group, blood flow to the middle compartment did not decrease(p=0.002) and slightly decreased in the distal compartment(p=0.001). Cutaneous blood flow was significantly higher in all compartments of the flap in BoTA group than in control group, 7 days after the flap elevation. In histopathologic examination, greater number of vessels were noted in the BoTA group than in the control group. Conclusion: Botulinum toxin A can increase the survival of the random pattern cutaneous flap in rats by preventing the sympathetic collapse of peripheral vessels.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.218-219
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• 2002

In the past, the development of pharmacokinetic/pharmacodynamic (PK/PD) models for quantitating the time course of drug responses was mainly based on two types of models, the empirical effect compartment model that simply accounts for the delay between effect and plasma concentration (hysteresis) and the mechanism-based so-called indirect response model. The first approach traces back to a paper by Segre (1) and its application was popularized by Holford and Sheiner (2); indirect response models were introduced by Jusko's group (3). (omitted)

• Animal cells and systems
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• 제7권2호
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• pp.169-171
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• 2003

Sodium, potassium, calcium, zinc and magnesium levels in the serum of 31 patients diagnosed as acute myocardial infarction were analyzed on admission (within 24 Hours) and after 48 hours. The results were compared with those of 26 age matched controls. No significant difference was observed in the mean sodium, potassium, calcium and zinc levels between the cases and controls. Compared to the controls, however, the variation in the level of magnesium is highly significant at the time of admission as well as after 48 hours. When the risk factors like diabetes mellitus, hypertension, smoking and alcohol were considered, it is found that there is no significant difference between the risk groups as well as between the patients. The alteration in magnesium level in acute myocardial infarction is independent of these risk factors. Within the first 24 hours, the significant decrease in serum magnesium (35-51％ fall when compared with the control group), correlates with its entry into the cell following ischemia. From this hypomagnesemic state, it rises to 9-22 times after 48 hours. This hyper-magnesemia after 45 hours is probably due to the shift of magnesuim from the intracellular fluid compartment to the extracellular fluid compartment that follows cellular recovery. Therefore, including magnesium in the immediate management of acute myocardial infarction will be beneficial in the early recovery.

• Korean Journal of Radiology
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• 제22권1호
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• pp.139-154
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• 2021

Magnetic resonance imaging (MRI) has become a crucial tool for evaluating mediastinal masses considering that several lesions that appear indeterminate on computed tomography and radiography can be differentiated on MRI. Using a three-compartment model to localize the mass and employing a basic knowledge of MRI, radiologists can easily diagnose mediastinal masses. Here, we review the use of MRI in evaluating mediastinal masses and present the images of various mediastinal masses categorized using the International Thymic Malignancy Interest Group's three-compartment classification system. These masses include thymic hyperplasia, thymic cyst, pericardial cyst, thymoma, mediastinal hemangioma, lymphoma, mature teratoma, bronchogenic cyst, esophageal duplication cyst, mediastinal thyroid carcinoma originating from ectopic thyroid tissue, mediastinal liposarcoma, mediastinal pancreatic pseudocyst, neurogenic tumor, meningocele, and plasmacytoma.

• Journal of Pharmaceutical Investigation
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• 제21권4호
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• pp.247-251
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• 1991

Effects of aluminum magnesium hydroxide (A) and cimetidine (C) on the pharmacokinetics of minocycline (M) were investigated in female rats. Blood samples were collected at various time intervals until 36 hrs following oral dosing of drugs. Plasma minocycline concentrations were determined by HPLC. Control group (M), $T_1$ group (M+A), $T_2$ group (A+M after 2 hrs), $T_3$ group (M+A after 2 hrs), $T_4$ group (M+C) and $T_5$ group (C+M after 2 hrs) were divided to examine interaction of the drugs with minocycline. Plasma minocyline level-time curves were well described by two-compartment open model with first-order absorption in rats. Antacid treatment was associated with reduced of 71.0, 45.9, 35.7% in minocycline absorption rate $constant(K_{\alpha})$, maximum plasma $concentration(C_{max})$, and relative $bioavailability(F_{rel})$, respectively. Cimetidine treatment group exhibited no significant changes in plasma level-time curve when compared with control group and did not affect minocycline absorption as by any of these three parameters.

• 대한수의학회지
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• 제35권3호
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• pp.471-480
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• 1995

The purpose of this experiment was to develop a simple and reliable HPLC method for the detection of ciprofloxacin in chicken serum and to provide a basic data on pharmacokinetic parameters after oral and intramuscular administration. The results obtained were as follows: 1. 0.2% meta-phosphoric acid: acetonitrile(7:3, v/v) solution had a high and regular recovery rates and was selected as an extraction solution. 2. The recovery rates of ciprofloxacin were 83-97% with the selected solution in chicken serum and the detection limit was 50ng/ml in serum. 3. Ka(abosorption rate constant) were 3.652 1/h in fasted group and 0.880 1/h in non-fasted group, and Ke (elimination rate constant) were 0.061 1/h and 0.133 1/h, respectively. 4. The highest concentration in serum after intramuscular injection was 840ng/ml within 15-30min and 160-324ng/ml in 1.1-3.2 hours after oral administration. 5. The time course of blood concentration fits well into a 2 compartment model. 6. On oral administration of ciprofloxacin with feed, ciprofloxacin was absorbed more slowly and the amount of absorbed was smaller than that of in fasted chickens. 7. Blood concentration of ciprofloxacin increased in a dose-dependent manner after intramusclular and oral administraiton.

• 대한방사선기술학회지:방사선기술과학
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• 제27권4호
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• pp.17-21
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• 2004

최근 의학 영상 기술의 발전과 함께 동적 골반 MRI(Pelvic magnetic resonance imaging)가 소개되었고 이러한 기술을 이용하여 동적 MR 배변조영술이 소개되어 환자의 진단에 도움을 주기 위해 사용되고 있다. 처음에 도입 당시에는 탈장(Enterocele)과 방광탈출증(Cystocele) 등을 진단하기 위하여 사용되었지만, 이후 장기의 탈출증(Prolapse), 또는 다른 골반 장기의 이상에 점점 그 유용성이 증명되어 현재는 기능성변비 등 다른 질환에서도 많은 보고가 있다. 본 논고에서는 골반 MRI 및 동적 MR 배변조영술에 대해서 소개하고 앞으로의 임상적 응용에 대한 전망을 살펴보도록 한다. 최근까지도 Pelvic MRI의 결과들은 앙와위(Supine position)에서 골반의 움직임을 관찰하는데 Yang 등은 26명의 골반 기관의 탈출증(Pelvic organ prolapse)을 가진 환자들을 관찰하고 Pubococcygeal line을 해부학적인 지표로 이용하여 좋은 결과를 얻었다고 보고했다. 이들은 Fast gradient recalled acquisition(fast GRASS)을 이용하여, Cystocoele, Prolapse, Enterocoele 그리고 Rectocoele 등을 증상이 없는 대조군과 비교하여 보고하였다. Kruyt 등은 Posterior compartment를 주로 관찰하여 MRI가 Fluoroscopy에 비하여 더 도움이 된다고 보고하였다. Healy 등은 Fast GRASS sequence를 이용하여 변비를 가진 환자와 변실금을 가진 환자, 그리고 증상이 없는 대조군을 대상으로 하여 동적 MRI 검사를 실시하였다. 이들은 변비나 변실금 증상과 관련되어 환자들의 Posterior compartment에 여러 곳에서 Prolapse을 관찰할 수 있었다. 이후 MRI 기술은 Lienemann 등에 의해서 더욱 발전했는데 그는 Fast T2 weighted turbo spin echo 기술을 이용하여 영상을 좀더 세밀하게 얻을 수 있게 되었다. 지금까지의 앙와위에서의 검사로 진단에 한계가 있었던 Intussusception 등의 질환을 Open MR 등의 방법으로 극복할 수 있다면 장래에는 방사선학적 배변조영술을 대체할 수 있는 검사법으로 발전할 수 있을 것으로 생각된다.

• Tuberculosis and Respiratory Diseases
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• 제52권3호
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• pp.241-250
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• 2002

연구배경 : 진폐증은 주로 분진흡입에 의해 폐실질에 섬유화를 일으키는 질환으로 만성염증에 의해 폐포내에 대식세포를 축적시키는 특정이 있으며, 크게 단순진폐증(SP)과 진행성 종괴성 폐섬유증(PMF)으로 구분된다. 본 연구의 목적은 진폐증 환자에서 질환의 심한 정도를 ILO 분류에 따라 SP, PMF로 구분한 후, 감염이나 기타 기저질환이 없는 상태에서 혈중 임파구 분획 및 싸이토카인의 농도를 비교한 것이다. 방 법 : 태백 중앙 병원과 영동 병원의 진폐증 환자 중 정밀 검사를 시행하기 위해 병원을 방문한 단순진폐증 환자와 입원 중이거나 정밀검사를 시행한 진행성 종괴성 폐섬유증 환자, 대조군을 대상으로 하였고, 대조군(C;10), 단순진폐증 (S;19), 진행성 종괴성 폐섬유증 (P;30명)에서 혈중 임파구 분획을, C(10), S(14), P(14명)에서 싸이토카인 (IFN-${\gamma}$, TNF-${\alpha}$, IL-6, IL-4))의 농도를 비교하고, 특히 흡연력이나 연령, 폐기능에 의해 변할 수 있는 싸이토카인의 변화를 동시에 고려하여 진폐증의 심한 정도와의 연관성을 관찰하였다. 결 과 : C, S, P 3 군간의 B-임파구, T, $T_H$, Ts, 및 NK-세포를 측정하였을 때 대조군과 단순진폐증환자 사이에서 T, $T_H$는 통계학적으로 의미있게 증가하였으며(P<.01) $T_H$는 두 군간에 의미있게 감소하였으나(P<.01), S, P 두 군 사이에는 특별한 변화가 없었다. 세 군 사이의 싸이토카인 분비 양상은 통계학적인 의의를 발견할 수 없었다. 결 론 : 혈청 임파구 분획은 C군과 비교하여 S군에서 T, $T_H$는 통계학적으로 의미 있게 증가하였으며 Ts는 감소하는 경향을 나타내어 진폐증의 진행 초기에 이들이 연관성이 있다고 추측되어진다.

• Asian-Australasian Journal of Animal Sciences
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• 제21권11호
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• pp.1642-1650
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• 2008

The objective of this study was to assess the effect of clinoptilolite (a natural zeolite) on growth and performance of broilers as well as on the quality of their litter. A total of 5,200 one-day old broiler chickens (Cobb 500) were used in two consecutive rounds lasting 42 days each. The broilers were given free and continuous access to a nutritionally non-limiting diet (in meal form) that was either a basal diet (B) or a 'zeolite diet' (Z; the basal diet supplemented with clinoptilolite at a level of 2%). A $2{\times}2$ factorial design consisted of two feeding treatments (B and Z) and two bedding types, sawdust (S) and sawdust with zeolite (Sz, which was the result of adding $2kg\;zeolite/m^2$), was used. In each round the broilers were randomly assigned to one of four (n = 650), treatment groups: two fed on the basal diet (B) and had bedding of either sawdust (group BS) or sawdust and zeolite (group BSz) and two fed on the Z diet and had as bedding either sawdust (ZS) or sawdust and zeolite (ZSz). Average growth rates were significantly (p<0.05) different between broilers of different groups; broilers that were fed on the 'zeolite diet' (Z) and were placed either in a compartment with sawdust bedding or sawdust bedding and zeolite (ZS and ZSz) as well those that were fed on the basal diet in a compartment with sawdust bedding and zeolite (BSz) grew at a faster rate (p<0.05) compared with those of the control group (BS). The incorporation of NZ in broilers diets and in their bedding material decreased the organic content in litter samples throughout the experimental period. The lowest organic content was recorded in group ZSz where NZ was added in both feed and litter. Mean ammonia concentration (ppm) was significantly higher in group ZS in comparison to groups BSz and ZSz (27.00 vs. 20.55 and 21.71 respectively). The results of this study showed that the incorporation of the clinoptilolite both in feed and into the litter had a positive effect on broiler growth and also on the quality of their litter.

• 약학회지
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• 제46권5호
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• pp.337-342
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• 2002

Propofol(2,6-diisopropyl phenol) is a phenol derivative that is chemically distinct from other intravenous sedative hypnotics. It has been extensively used as a short term anesthetic agent, because of the rapid onset and short duration of action. Propofol microemulsion system was prepared with different concentrations of ethyl oleate, $Solutol^{(R)}$ HS 15 and $Kollidon^{(R)}$ 17 PF. Propofol microemulsions were studied by transmittance, viscosity, particle size, in vitro release and pharmacokinetics. The range of transmittance of A group with 4% ethyl oleate and that of B group with 5% ethyl oleate were 92.6~95.1 and 91.3~94.2%, respectively. Transmittance 1~2% decreased as concentration of $Kollidon^{(R)}$ 17 PF was increased and increased 0.8~3.3% when 10 times diluted with normal saline. The viscosity of A and B group were in the range of 3.9~4.1 mPaㆍsec and 4.4~5.3 mPaㆍsec, respectively. The particle sizes of A and B group increased as amount of $Kollidon^{(R)}$ 17 PF. Also, release of propofol was slowly increased as the amount of $Kollidon^{(R)}$ 17 PF was increased. Propofol plasma concentration by i.v injection showed 2-compartment model. Pharmacokinetics of A-5 was similar to that of commercial emuision(POFOL).