• Journal of Plant Biotechnology
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• 제6권4호
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• pp.229-233
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• 2004

Partial desiccation of embryogenic calli cultures or somatic embryos leads to different physiological changes and maturation of somatic embryos, leading to improved plant regeneration. Embryogenic calli was induced from immature inflorescence segments and young leaf rolls of sugarcane (Saccharum officinarum hybrids CoC-671) on Murashige and Skoog's basal medium enriched with different concentrations of 2,4-D ($1-4\;\cal{mg/l}$), L-glutamine ($100\cal{mg/l}$), malt extract ($100\cal{mg/l}$), casein hydrolysate ($1000\;\cal{mg/l}$) and coconut milk ($5\%$) and solidified with $0.2\%$ gel rite. The embryogenic calli were subjected to desiccation for 1-8 h. Desiccation of the calli for 6-7 h resulted in enhancement of plant regeneration frequency ($83-96\%$) as compared to control ($12\%$). Plantlets exhibited vigorous growth to maturity in the greenhouse. Partial desiccation of embryogenic calli offers as a simple method for improving plant regeneration frequency in sugarcane.

• 한국자원식물학회지
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• 제24권3호
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• pp.280-285
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• 2011

In this study, we established a novel somatic embryogenesis and plant regeneration system through cell suspension culture of white dandelion (Taraxacum coreanum NAKAI.). Embryogenic calli could be initiated from leaf and root explants of sterile seedlings on solid Murashige and Skoog (MS) medium supplemented with 1.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) after 3-week cultures. To proliferate embryogenic calli rapidly, cell suspension culture was performed with transferred to liquid MS medium with various combinations of plant growth regulators (PGRs) including 2,4-D, ${\alpha}$-naphthalene acetic acid (NAA), indole-3-acetic acid (IAA), $N^6$-benzylamino purine (BAP), thidiazuron (TDZ), and kinetin. During suspension cultures, embryogenic calli not only greatly proliferated, but shoot organogenesis also simultaneously occurred from the surface of somatic embryos. Among them, TDZ at lower concentration, 0.1 mg/L produced the highest efficiency of somatic embryo formation and shoot organogenesis. Rooting of embryogenic calli with adventitious shoots was done on solid MS medium containing 0.1 mg/L NAA and 0.3% activated carbon. Nearly 80% of embryogenic calli with shoot organogenesis could be rooted normal. Well-rooted plantlets were transferred into pots under a greenhouse condition, and plants derived from this system appeared phenotypically normal.

• 원예과학기술지
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• 제31권4호
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• pp.423-428
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• 2013

Although closed soilless culture is useful for saving water and fertilizers with minimizing environmental pollution, adequate management of nutrient solutions is still not stabilized in greenhouse cultivation. In order to investigate the problems occurred in closed soilless culture of Paprika (Capsicum annuum L., cv. Fiesta), we compared ion balance, fruit yield, and the water and fertilizer use efficiencies in the closed system with those in the open system. The plants were grown in rockwool culture with a nutrient solution of EC $2.5dS{\cdot}m^{-1}$. After 4 weeks of treatment, individual ratio of $NO{_3}^-$, $K^+$, $Ca^{2+}$, and $Mg^{2+}$ to total ion concentrations ($meq{\cdot}L^{-1}$) decreased from the initial value, especially the biggest decrement was observed in $K^+$, and on the other hand, $SO{_4}^{2-}$, $Cl^-$, and $Na^+$ were accumulated in the closed system. Yields after four-time harvests were 19% higher in the open system than in the closed system. Total volume of water used per unit area ($m^2$) in the open system was 20% higher, but the total water use per fruit was not significantly different between the two systems, while t total fertilizer use per fruit was 78% higher in the closed system. Amount of marketable fruits was not significantly different between the two systems. We concluded that the increase in $K^+$ supply and the replenishment of recycled nutrient solution every four weeks were required for preventing the imbalance or depletion of nutrients in the close soilless culture of paprika plants to get more balanced nutrient composition during whole cultivation period.

• 식물조직배양학회지
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• 제22권3호
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• pp.131-135
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• 1995

잡종 사기나무의 기내 대량증식을 목적으로 줄기절간 유래의 기내 및 기외시료를 이용하여 다경줄기 유도에 관여하는 식물생장 조절물질의 효과에 대하여 조사하였다. 기외시료의 측아를 제거한 다음 20에서 30$\mu$M 의 zeatin이 함유된 WPM 배지에 6주간 배양하였을때 각각 11개와 13개의 다경줄기가 유도되었다. 그러나 기외시료의 측아가 붙어있는 상태에서 기내배양하여 2주후에 측아를 제거하고 동일 배지에 배양하였을 경우에는 BA가 1.0에서 2.0 $\mu$M 함유된 배지에서도 13개와 15개의 다경줄기를 유도할 수 있었으며, 특히 이때 생산된 줄기의 상태는 아주 건전한 것으로 나타났다. 기내줄기를 사용하였을때는 20 $\mu$M의 zeatin을 처리하였을때 가장 많은 줄기를 생산할 수 있었으며, 생산된 조직배양묘는 일정기간 순화를 시킨 후 온실이나 포지에 식재가 가능하였다.

• 한국농림기상학회지
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• 제20권2호
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• pp.183-189
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• 2018

고측고 토마토 전용 하우스에서 비순환식 토마토 암면재배 시 배지 내 수분함량이 토마토 '데프니스' 품종의 생육 및 과실의 생산성에 미치는 영향을 보고자 수행하였다. 양액공급량은 일사량제어가 FDR센서 수분제어보다 3.8배 많았다. 일사량제어의 수분함량은 90-95%였고, FDR센서 수분제어의 수분함량은 60-65%였다. 초장의 길이 및 토마토의 과중은 일사량 제어가 FDR센서 수분제어보다 1.2-1.9배 길었고, 1.2-2.0배 무거웠다. 상품과는 일사량 제어가 FDR센서 수분제어보다 1.3배 많았다. 과실의 당도는 처리간 차이가 없었다. 열과 발생량은 일사량 제어가 FDR센서 수분제어보다 2.0배 높았다. 따라서 비순환식 암면 배지에서 토마토의 생육 및 생산성을 향상시키기 위해서는 일사량제어가 적당하다고 판단된다.

• 생명과학회지
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• 제23권8호
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• pp.1010-1018
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• 2013

본 연구에서는 온실가루이 방제를 위하여 감염된 이병체로부터 균주 분리 및 농촌진흥청 및 경상북도농업기술원에서 균주를 분양 받아 5 종의 균주를 확보하여, 최종적으로 활성이 가장 높은 분리균주 M130을 선발하였다. Beauveria bassiana M130을 이용하여 균주가 갖는 효소학적 활성을 분석하였다. Chitinase와 protease의 활성을 조사한 결과, 타 균주에 비하여 높은 효소학적 활성을 가지는 조건을 얻었으며, 선발된 균주의 포자생산의 최적화를 위한 방안으로 PDB (potato dextrose broth)배지와 미강추출물을 이용한 ERBM (extracted rice bran medium)을 비교하여, 더 높은 생육도와 포자 생성량을 보인 ERBM을 본 균주를 배양하는 기본배지로 선발하였다. ERBM을 이용한 액체배양시의 최적조건으로, 기본배지인 ERBM에 0.5% $(NH_4)_2SO_4$를 첨가하여 pH 5, $28^{\circ}C$에서 배양최적화를 하였다. 향후 곤충병원성곰팡이 Beauveria bassiana M130의 최적화 조건에 따라 배양을 한다면 포자의 생산량 및 방제효과가 높아질 것으로 사료된다.

• 한국자원식물학회지
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• 제6권2호
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• pp.171-179
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• 1993

This study was conducted to investigate the effect of growth regulators and medium composition on the growth of each stage in apical meristem culture for mass propagation of Zanthoxylum piperitum var. inerme Makino. The source material, shoot tip segments were taken from three-years old graft trees. Apical meristems were cultured in vitro on basal MS, GD, WS, half strength MS(1/2MS) and half strength GD(1/2GD) media supplemented with various concentrations for growth regulators(BA, IBA) and inorganic nutrients. The results summarized are as follows: 1. In culture establishment stage, ratio of culture establishment was 96.7% and the best resuit was obtained using MS medium supplemented with 1.0mg/l BA and 0.2mg/l IBA. 2. In shoot multitication stage, both shoot multiplication and growth were achieved in average 5.6cm. These results were obtained on in MS medium supplemented with 1.0mg/l BA and 0.2mg/l IBA. 3. In roothing stage, phloroglucinol(PG) acted as IBA synergist in root initiation. The most faverable combinations for root development was half-strength MS medium supplemented with 162mg/l PG and 0.2mg/l IBA, and ratio of rooting was 58.0%. 4. In Vitro formed plantlets were transplanted to paper pots in greenhouse with 85% of relative humidity. 96% of survival rate was obtained from artificial soil mix having same volume of sand, vermiculite, peat, and soil.

• 한국농공학회지
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• 제37권1호
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• pp.55-64
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• 1995

This paper presents analytic results of energy sequestered for the forcing cultural Cu- cumber and the others production system with the input-output tables method in the suthern parts of Korea. In this study an attempt is made to evaluate input of direct and indirect energy, output of yield energy and net energy in order to achieve increased energy productivity under P E greenhouse. Cultural practices were grouped soil and soilless with perlite for vegetable production. The results from this study are summarized as follows : 1. Total energy inputs in cucumber production were calculated to be 510 GJ/l0a(di- rect energy : 480 GJ/lOa, indirect energy : 30 GJ/lOa) from soil culture and 440 GJ/ 10a(direct energy : 420 GJ/lOa, indirect energy : 20 GJ/lOa) from soilless culture in perlite hydroponics. 2. Energy outputs from cucumber and biomass were 7 GJ/lOa and 120 GJ/lOa at a uniform rate respectively. 3. Heating fuel as diesel is a major energy inputs approaching 90% of the total energy requirements for cucumber production. 4. Net energy in cucumber production was calculated to be 503 GJ/lOa from soil cul- ture and 431 GJ/lOa from soilless culture. Net energy productivity was maintained costantly as 0.98. 5. Energy productivity in cucumber was calculated to be 0.029 kg/MJ from soil culture and 0.043kg/MJ from soilless culture, while energy efficiency was 0.012 and 0.015 respectively. It is expected that a soilless cultural production system seems to be reduc- tive in seguestered energy input by 13%.

• 자연과학논문집
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• 제11권1호
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• pp.105-111
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• 1999

본 연구는 현재 재배되고 있는 주요 품종들에 대한 생육특성을 조사하고 휴면정도에 따른 보온개화기를 파악함으로써 딸기의 작형분화 양상과 이에 알맞은 품종을 제시하고자 실시하였다. 품종별 화아분화기는 "초동" "슈꼬" "여봉"이 9월 20일 ~ 9월 22일, "풍향" "여홍" "보교조생"등이 9월 26일 ~ 9월 30일, "모리오까"는 10월 19일이었고 휴면정도가 짧은 "여봉" "여홍" "풍향"등은 촉성작형, 휴면정도가 보통인 "수홍"은 준촉성, 휴면정도가 긴 "보교조생"은 반촉성 및 조숙작형, 그리고 아주 긴 "모리오까"는 노지 또는 억제작물에 적합한 것으로 사료되었다 .

• Journal of Forest and Environmental Science
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• 제37권4호
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• pp.323-330
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• 2021

Zygotic embryo culture was performed to propagate evergreen oak, Quercus myrsinifolia, which has recalcitrant seeds and is difficult to propagate by cuttings. Zygotic embryos appeared in WPM medium after 14 days, and after 56 days, they developed into complete plants with cotyledons and roots. The medium suitable for zygotic embryo culture was 1/4 WPM medium, showing a shoot growth of 2.43 cm and root growth of 8.7 cm after 8 weeks of culture. As a result of investigating the effect of GA₃ on the growth of plants germinated from zygotic embryos through GA₃ treatment, the best growth was shown in 0.5 mg/l GA₃ treatment. The in vitro rooting and growth of IBA-treated zygotic embryo-derived plants were good in the 0.5 mg/l IBA treatment and rooting and shoot growth were not observed at higher concentrations. And the callus induction rate also increased as the concentration of IBA increased. Plants grown in vitro were transferred to a plastic pot containing artificial soil and acclimatized in a greenhouse for about 4 weeks, resulting in more than 90% survival. As a result of this study, the zygotic embryo culture method was confirmed to be effective for mass propagation of Q. myrsinifolia. The results of this study are expected to contribute significantly to the mass propagation of elite Q. myrsinifolia.