• 생명과학회지
• /
• 제17권2호통권82호
• /
• pp.293-297
• /
• 2007

형광단백질 (green fluorescent protein, GFP)은 생물공정을 살피데 지표 단백질로 유용하게 사용이 된다. 본 연구에서는 쌀세포에서 외래 단백질의 발현양상을 관찰하기 위해서, 표지 단백질로 GFP를 형질전환 후 이것에서 유도된 현탁세포에서 GFP의 발현 양상을 관찰하였다. 형질전환시 GFP의 발현을 위한 프로모터로 RAmysE를 사용하였으며 이것은 배양액 중에서 당이 고갈되었을 때 강력히 작동된다. 그래서 본 연구에서는 배양액 중에 다양한 슈크로오스 농도로 쌀세포를 배양하여 세포의 성장양태 및 GFP의 발현양에 미치는 영향을 관찰한 결과 세포의 성장은 12%의 당농도에서 7.06g/L로 최적이였으며 GFP는 당을 가장 적게 사용한 3%에서 최적임을 알 수가 있었다. 이것은 세포의 성장과 GFP의 생산에 사용된 당이 반대로 영향을 미침을 알 수가 있었으며 향후 최적의 대량배양을 위해서는 세포의 성장과 산물의 생산시기를 분리한 이단계 배양법이 필요함을 암시한다.

• 한국토양비료학회지
• /
• 제46권3호
• /
• pp.187-192
• /
• 2013

This study was carried out to investigate the incorporation effect of green manure crops (GMC) such as the hairy vetch on improvement of soil environment in reclaimed land during silage corn cultivation over the past two years. Plots consisted of conventional fertilization (CF) and incorporation of GMC were divided by addition rate of nitrogen fertilizer (100 kg $ha^{-1}$) with 30 - 100% of non nitrogen fertilization (NNF). Soil physico-chemical properties and growth and yield potential of silage corn were examined. The tested soils showed strong alkali and saline properties with low contents of organic matter and available phosphate while contents of exchangeable sodium and magnesium were high. Soil salinity increased during cultivation of summer crop. However, corn was not affected by salt content. The fresh weight of GMC at incorporation time was 18,345 kg $ha^{-1}$. Content of total nitrogen was 3.09% and the C/N ratio was 12.8 at incorporation time. Fresh and dry matter yield of silage corn were higher in the order of N30% reduction, CF, N50% reduction, N70% reduction, N100% reduction and NNF. Fresh and dry matter yield potential of silage corn for N30% reduction were comparable to those of CF. Bulk density of the soil decreased with incorporation of GMC, while porosity was increased. The soil pH decreased while content of exchangeable calcium, available phosphate, and organic matter increased. Also contents of exchangeable sodium and potassium decreased with incorporation of GMC. The data indicate that incorporation of hairy vetch can improve soil physical and chemical properties and reduce nitrogen fertilizer application especially for alkali saline reclaimed soil such as Saemangeum reclaimed land.

• Journal of Biosystems Engineering
• /
• 제35권2호
• /
• pp.138-145
• /
• 2010

An on-line algorithm that discriminates brown rice kernels on their echelon feeder using color image processing is presented for quality inspection. A rapid color image segmentation algorithm based on Bayesian clustering method was developed by means of the look-up table which was made from the significant clusters selected by experts. A robust estimation method was presented to improve the stability of color clusters. Discriminant analysis of color distributions was employed to distinguish nine types of brown rice kernels. Discrimination accuracies of the on-line discrimination algorithm were ranged from 72% to 85% for the sound, cracked, green-transparent and green-opaque, greater than 93% for colored, red, and unhulled, about 92% for white-opaque and 67% for chalky, respectively.

• 한국식품과학회지
• /
• 제3권3호
• /
• pp.193-210
• /
• 1971

Reviewed biosynthetic pathways in green leaves of thiamine, riboflavin and folate as disclosed by the authors, and the regulatory systems operating on their biosynthesis and biodegradation as revealed since a potent inhibitory activity of ATP was found for the phosphatase reactions in 1965. Physiological roles of ascorbic acid in plant were evidenced by its higher content in green and flower leaves; the content in persimmon leaves for example was found ten or more times of that in citrus fruits. A close relationship existing between basic and development researches was illustrated by examples chosen from the author's researches. Examples cited were as follows; basic researches on vitamins were extended to the creation of thiamine enriched rice, to the growth promotion of rice plant by foliar application of thiamine, and to the use of ascorbic acid to prevent the appearance of beer cloudness; histological and biochemical studies on protein bodies in rice endosperm turned out amino acid enriched rice; techniques acquired in the studies on catalase were successfully employed for the utilization of single cell protein (MIPRON) for human food; conception of hybernation was led up to under-water or under-ground storage of cereal grains.

• 한국환경농학회지
• /
• 제29권2호
• /
• pp.109-114
• /
• 2010

수확기 벼의 간장은 모든 처리구에서 비슷하게 생육하였으나 수장은 녹비환원구의 70% 시비구(17.5 cm)에서 관행재배구(16.4 cm)보다 촉진시켰다. 수확기 벼의 지상부 잎과 줄기의 생체중은 유채 잔유물 녹비환원구의 30% 시비구에서 관행재배구와 비슷한 수준이었다. 벼의 건물중에서도 생체중과 비슷하였으며, 녹비환원구의 70% 시비구에서는 이삭중을 19% 증가시켰다. 또한 유채 잔유물의 환원에 따른 벼의 주당수수와 1수영화수는 관행재배구에 비해 녹비환원구의 70% 시비구에서 비슷하거나 오히려 증가하였다. 한편 벼의 등숙율은 관행재배구의 91%와 비교하여 유채 잔유물 환원구에서 낮아지는 것(79-85%)으로 나타났으나, 정조수량은 유채 잔유물 환원구의 70% 시비구에서 496.4 g/pot으로 가장 높았다. 각 처리구별 벼의 수량으로부터 산출한 수량지수는 녹비환원구의 70% 시비구에서 125.4로 관행재배구보다 수량이 증가하였다. 잡초발생량은 관행재배구에서 37본으로 가장 많았으며, 유채 잔유물 환원구에서는 11.7-15.7본으로 관행재배구보다 현저히 감소시켰다. 잡초의 건물중은 관행재배구의 25.1 g/pot에 비해 유채 잔유물 환원구의 시비량이 많은 처리구부터 각각 11.8, 12.2 및 6.0 g/pot으로 현저히 감소시키는 것으로 나타났다.

• 한국토양비료학회지
• /
• 제38권6호
• /
• pp.340-344
• /
• 2005

수년간 고추를 연작한 비닐하우스에서는 연작 또는 염류집적에 따른 수량감소가 현저하게 나타난다. 고추의 수량감소를 줄이면서 설치한 비닐하우스의 사용연한을 연장시킬 수 있는 수단으로 기존 토양에 왕겨를 혼합한 소토양관비재배의 가능성을 검토하였다. 소토양관비재배 시험구에서는 관행재배 시험구에 비하여 토양 EC가 $4dS\;m^{-1}$ 이하로 낮아졌으며 두 재배방법 사이에 지상부 생육의 차이는 크지 않았으나 뿌리의 발달은 소토양관비재배 시험구에서 현저히 좋았다. 특히 소토양관비재배 시험구에서는 잔뿌리의 발달이 많았으며 관행재배 시험구에서는 뿌리 양이 적을 뿐만 아니라 굵은 뿌리가 많이 발생되었다. 풋고추 수량은 소토양관비재배 시험구에서 43% 정도 증가되었는데, 이는 소토양관비재배 시험구에서의 양호한 뿌리 발달이 양분흡수에 기여하였고 결과적으로 수량 증대로 이어진 것으로 판단된다. 본 논문은 초기 단계의 연구결과이며, 소토양관비재배방법이 연작 토양 시설물의 이용효율을 극대화할 수 있는 방법의 하나로 그 가능성을 제시하였을 뿐이다. 소토양관비재배방법의 실용화를 위해서는 보다 구체적인 연구가 더 진행되어야 할 것이다.

• 한국식품영양과학회지
• /
• 제46권6호
• /
• pp.765-770
• /
• 2017

본 연구는 소비자가 추구하는 기능성 식품의 다양한 개발을 위해 녹차를 쌀밥에 접목시킴으로써 녹차의 생리활성이 부여된 기능성 쌀밥의 개발 가능성 검토를 위해 실시하였다. 녹차분말을 첨가한 쌀밥의 항산화 활성과 생리활성 물질의 변화 및 물성 변화를 알아보기 위해 취반 시 사용되는 물에 녹차분말을 1, 2, 3%로 첨가하여 취반하였다. 이후 제조된 밥은 동결건조 후 메탄올 추출물을 이용하여 항산화 성분 및 항산화 활성을 분석하였다. 또한, 취반 후 물성을 측정하여 그 변화를 알아보았다. 취반 후 녹차분말의 함량이 증가할수록 폴리페놀과 플라보노이드의 함량이 농도 의존적으로 증가하는 경향을 보였다. 쌀밥의 항산화 활성은 첨가한 녹차분말의 농도에 의존적으로 ABTS 및 DPPH 라디칼 제거능력이 증가하는 경향을 보였으며 환원력 및 지질과산화억제 효과에서도 첨가된 녹차분말에 의존적으로 활성이 증가하였다. 반면 녹차분말을 첨가한 쌀밥의 물성을 측정한 결과 녹차를 첨가하였을 때 경도가 증가하는 경향을 보였으나 유의적인 차이를 보이지 않았다. 이 연구 결과를 통해 녹차분말을 첨가하여 취반할 경우 식감을 유지하면서 녹차의 생리활성 물질도 부여할 수 있어 기능성 쌀 가공식품으로서의 적극적인 활용이 가능할 것으로 생각된다.

• Applied Biological Chemistry
• /
• 제27권
• /
• pp.56-67
• /
• 1984

As in many other country, the use of organic matter in Korea has long history. Farmers understand the value of organic matter as the source of plant nutrient and soil improving agent in general. Since 50 years ago, the sources of organic matter in paddy soils were compost, rice and barly straw, green manure, animal waste, fish and beancake, etc.. Application of green manures such as vetch and chinese milk vetch showed no significant effect on the yield of brown rice in paddy soil. On the other hand, the effects of compost and rice straw showed more significant on the yield of brown rice in paddy soil. Application of rice straw in rice cultivation is commonly made at different times between harvest, early spring and several weeks before transplanting. Considering the suitable paddy soil for application of rice straw under well to moderately well drained soil, the yield was pronounced more than poorly drained soil. Based on laboratory and field experimants, application of rice straw promoted the decrease of oxidation-reduction potential in well to moderately well drained soil. This results to be enhanced the release of some mineral nutrients,. such as potassium, calcium, silicon, and increase of availability of soil phosphorus. In the field experiments, results obtained from nitrogen fraction on the immobilization-mineralization of the tracer nitrogen applied in paddy soil,the amount and index of organic nitrogen incoporated in soil was more pronounced in rice straw application than control. Rice straw and its transformation products incoporated in the soil, provided the inflow of energy necessary to maintain heterotrophic microbes activities. Rice straw and its transformation products, especially soluble carbohydrate, enhanced the population of free-living heterotrophic $N_2$ - fixing microbes. Moreover, rice straw and its transformation products in paddy soil, enhanced the activities of soil enzymes such as dehydrogenase and urease.

• 한국유기농업학회지
• /
• 제20권2호
• /
• pp.143-159
• /
• 2012

Agricultural income is calculated with producer price, output and management cost. This study compared organic farming with conventional one for agricultural income, producer price and output by items. And then it proposed the method of item selection and crop system from a diversification point of view. The coefficient of variation to producer prices in organic farming was 4.7%, and conventional one was 30.3% because organic products have been produced in a system of contract farming with consumers' cooperative. This result means the price of organic products is stabler than that of conventional price. And agricultural income of organic farming has been generally known more than that of conventional one. However, agricultural gross income of conventional farming was more than that of organic one by 20.3% in 2010. It was caused by output reduction of a few items(fer example; onion, large green onion, potato and young pumpkin) due to freak weather conditions and constant producer price for several years in organic farming. In order to increase agricultural income, appropriate crop selection and system should be introduced to organic farming. A principal crop is the rice plant and 2 subordinate crops are dry crops at bare field and greenhouse respectively. Thus 5 crop systems that agricultural gross income are relatively increased larger among 15 crop systems estimated are rice+ginger+cucumber, rice+ginger+tomato, rice+large green onion+cucumber, rice+sweet potato+cucumber and rice+onion+ cucumber.

• 한국자원식물학회지
• /
• 제34권6호
• /
• pp.517-526
• /
• 2021

Brown rice is more nutritious than the commonly consumed white rice, however, the former is not widely accepted because of its coarse texture and difficulty in cooking. Germination is an easy technique to minimize the drawback of normal brown rice. The objective of this study was to investigate the effect of three different concentrations (1, 3, and 5% w/v) of turmeric extracts on the nutrient and antioxidant activities of germinated brown rice (GBR). The b (yellowness) values of the turmeric-treated GBR were significantly higher than that of the untreated control. Lower concentration (1%) slightly increased (3395.5 mg/kg) but higher concentrations (3 and 5%) reduced (1735.8 - 2393.7 mg/kg) the total mineral content in GBR, as compared to the control (3377.4 mg/kg). The amount of essential, non-essential, and total amino acids, including GABA, were increased with the concentration of turmeric extracts. The amount of essential amino acids was increased by 58.3, 71.5, and 88.3% with the application of 1, 3, and 5% extracts, respectively. The antioxidant potential of GBR was also enhanced with turmeric treatment. Overall results indicated that 1 or 3% turmeric treatment could be appropriate to enhance the nutritional and functional value of GBR.