• KOREAN JOURNAL OF CROP SCIENCE
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• v.54 no.4
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• pp.384-389
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• 2009

Germination characteristics of Chinese milk vetch(CMV) seeds produced from Hanam Province, China and Milyang, Korea were investigated to give basic information on the stability of seedling establishment in the CMV cultivation. The germination percentage of the imported CMV seed from China varied according to importation year and seed collection site ranging from 79~95%. The germination of black colored seed coat was lower than the light green colored ones and germination by seed weight was not significantly different. Although the seed germination was lower under dark than in the presence of light, it was not significantly different. The germination of the imported CMV seed slightly declined to only less than 6% after one-year of storage under natural environment conditions but it significantly decreased after two years. However, when the seed was stored at the $5^{\circ}C$, the seed germination was the same as after two years of storage. On the other hand, fresh CMV seed produced in Milyang, Korea had only 8% germination due to seed coat dormancy but the germination increased to 73~85% after breaking seed dormancy after a year of storage. The high germination percentage of 72~82% was still maintained even after 27 months of seed storage unlike the CMV seed produced from China. These results indicate that CMV seeds do not require light for germination and the seed from China should be used within one-year after importation while the seeds produced from Korea can be used even after two years from harvest for stable CMV seedling establishment in the CMV-rice cropping system.

• The Korean Journal of Pesticide Science
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• v.19 no.4
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• pp.370-393
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• 2015

This study was conducted to compare STQ method, multi-residue method in Korean food code and QuEChERS method for validated selected and accuracy, reproducibility and efficiency. A total of 45 selected and targeted pesticides were the analyzed by GC and 5 of them were crops (apple, potato, green pepper, rice, soy bean). $R^2$ values were calculated in the standard calibration curve was over 0.990. Recovery tests were performed by three replications in two levels and the relative standard deviation of the repeated experiments was less than 30%. The average percentage of recoveries in the multi-residue method in Korean food code was 89.13%, QuEChERS method was 92.45% and STQ method was 85.28%. In addition, matrix effects in multi-residue method in Korean food code was 24.61%, QuEChERS method was 23.98% and STQ method showed 11.24%. The STQ method is easy and showed high clean-up effect in extracting the sample solution than the QuEChERS method and clean-up with C18, PLS, PSA cartridge columns. A large volume of the sample was injected in order to compensable for the problem, that occurred due to high detection limit in the analyser. When the STQ method was applied using a large volume injector, the standard calibration curve showed a higher linearity $R^2=0.990$, and method detection limit was 0.01 mg/kg. It showed an average recovery of 91.84% and the relative standard deviations of three replications repeated in two level process was less than 30% and had an average matrix effect of 17.90%.

• Research in Plant Disease
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• v.16 no.1
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• pp.27-34
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• 2010

This study was performed to develop a formulation using an antagonistic bacterium Bacillus amyloliquefaciens A-2 to control tomato leaf mold caused by Fulvia fulva. B. amyloliquefaciens A-2 was grown in a medium with rice oil and mixed with various carrier and additives. One of the formulations, A2-MP, showed the best disease control value among the tested formulations. The disease control value of A2-MP at 100-fold and 500-fold diluted treatment was not significantly different from that of chemical fungicide triflumizole in a growth chamber. Although disease control effect was decreased by serial diluted treatment of the prepared A2-MP, 1,000-fold diluted treatment of A2-MP still showed high disease control value of 72.0%. For the green house experiments, the disease control values of A2-MP was indicated as 79.4% which is similar to that of chemical fungicide, triflumizole showing 79.6%. When the disease control activity of the formulation A2-MP was compared in tomato production conditions, disease control values of 100-fold diluted A2-MP and 3,000 fold diluted triflumizole exhibited 60%, 81.6%, respectively. The disease control efficiency by A-2MP was 73% of the disease control value of chemical fungicide. The formulation A-2MP maintained the stable bacterial viability and disease control activity when stored at $4^{\circ}C$. This result suggested that A-2MP develped from B. amyloliquefaciens A-2 could be used to control tomato leaf mold.

• Journal of the Korean Society of Food Science and Nutrition
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• v.43 no.3
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• pp.341-348
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• 2014

We investigated the anti-obesity and anti-hyperlipidemic effects of extracts of two roasted coffee beans (Vietnam robusta and Ethiopia mocha sidamo G2) and fermented coffee beans with Monascus rubber mycelium (MR) by solid-state culture. C57B/L6 mice were divided into seven groups: normal diet (ND) group, high fat diet (HFD) group, and HFD groups with hot water extracts from Vietnam robusta coffee beans (HFD-VR), MR-fermented Vietnam robusta coffee beans (HFD-VR-MR), MR-fermented Vietnam robusta coffee beans with 10% brown rice (HFD-VR-MR-BR10), Ethiopia mocha sidamo G2 coffee beans (HFD-ES), and MR-fermented Ethiopia mocha sidamo G2 coffee beans (HFD-ES-MR). After 6 weeks, body weight gain and food efficiency ratio were higher in the HFD group, but significantly reduced in the coffee extracts-fed groups. The HFD-ES-MR group showed greater body weight reduction than the HFD-ES group. The serum triglyceride, total cholesterol, and LDL-cholesterol levels as well as the atherogenic index and cardiac risk factor all tended to decrease in groups fed Vietnam robusta coffee extracts compared to the HFD group. These results suggest that Vietnam robusta and Ethiopia mocha sidamo G2 may be used to make functional coffee beverages with anti-obesity and anti-hyperlipidemic activities.

• The Korean Journal of Pesticide Science
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• v.15 no.3
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• pp.254-268
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• 2011

Fenoxycarb, pyriproxyfen and methoprene are juvenile hormone mimic insecticide. These insecticides have been widely used for mosquito, fly, scale insects, and Lepidoptera. The purpose of this study was to develop a simultaneous determination procedure of fenoxycarb, pyriproxyfen and methoprene residues in crops using HPLC-UVD/MS. These insecticide residues were extracted with acetone from representative samples of four raw products which comprised brown rice, apple, green pepper, and Chinese cabbage. The extract was diluted with saline water, and then n-hexane/dichloromethane partition was followed to recover these insecticides from the aqueous phase. Florisil column chromatography was additionally employed for final clean up of the extract. The analytes were quantitated by HPLC-UVD/MS, using a $C_{18}$ column. The crops were fortified with each insecticide at 3 levels per crop. Mean recovery ratios were ranged from 80.0 to 104.3% in four representative agricultural commodities. The coefficients of variation were less than 4.8%. Quantitative limit of fenoxycarb, pyriproxyfen, and methoprene was 0.04 mg/kg in crop samples. A HPLC-UVD/MS with selected-ion monitoring was also provided to confirm the suspected residues. The proposed simultaneous analysis method was reproducible and sensitive enough to determine the residues of fenoxycarb, pyriproxyfen and methoprene in the agricultural commodities.

• Korean Journal of Weed Science
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• v.13 no.2
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• pp.138-150
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• 1993

Experiments were carried out to understand how much do the environmental factors affect growth and tuber formation of arrowhead, Sagittaria trifolia L. The more the light transmittance decreased, the more the numbers of leaves and floral axes decreased. The dry matter weight of tops and the number and the fresh weight of formated tuber were significantly different between the light transmittance of more than 50% and that of less than 30% at the 5% level of DMRT. Plant height, number and width of leaves, and number of floral axis were affected by the Light spectra. And the degree of their effects on growth of arrowhead was different form the light spectrum. The natural light and the clear cellophane film were the most effective to increase the number and the fresh weight of formated tuber and the green spectrum was the least effective to do those. Plant height grown at 0-5cm water depth was shorter than that at 10-20cm water depth. The deeper the water depth was, the lower the leaves number was. The fresh weight and the number of arrowhead tuber were most produced at 0cm water depth and theose were least at 20cm water depth. The shoot growth and the tuber formation of arrowhead was much increased with increase of the application rate of fertilizer. The difference of the transplanted tuber size was not affected at the shoot growth, but tuber formation of arrowhead was increased with increase of the transplanted tuber size. From viewing the effect of temperature after rice heading, the shoot growth and the tuber formation at $35^{\circ}C$ were also higher than those at $25^{\circ}C$.

• Korean journal of food and cookery science
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• v.7 no.1
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• pp.67-80
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• 1991

This study researched the dietary habit of boy's and girl's high school students, and into low it was connected with the state of health between the group of having the right dietary habit and the group of having the wrong habit. Results were as follows: 1. The age about the object person of research was the most numerous in 79% at 16~17 years old, the average weight was $55.67\pm9.08$kg, the average height was $165.47\pm7.56$cm. 2. In the parent's school career of the object person of research, persons who graduated high school were many. The mother's educational level was lower than the father's school career. The parent's school career in the district south of a river was higher than the parent's school career in the district north of a river. And the parent's school career of cultural students was high. 3. The frequency of food intake in the district north of a river and in the district south of a river was a meaningful difference in the vegetables blended in green and yellow things (p<.01), fruit (p<.05), rice, flour, potatoes (p<.05), and so the district north of a river took less than the district south of a river. 4. The most regular diet in a day was lunch. The appetite of the students was generally good. 5. The general environment and state of health were a meaningful difference about melancholia (p<.05) in the district of north and south of river, and so melancholia in students of the north of a river was high. 6. The state of health according to regular diet was a meaningful difference, so regular student was better than irregular student in state of health, and was the same in study. 7. Eating habit correlated much to each state of health, Especially in physical health, the muscular frame correlated to fruit (p<.001), seaweeds (p<.05), fried food, jun, panbroiled food (p<.05), salty taste (p<.05), sour taste(p<.001).

• Korean journal of food and cookery science
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• v.26 no.6
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• pp.745-752
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• 2010

The purpose of this study was to investigate the perceptions and preferences held by the general public concerning jeungpyun. Self-administered questionnaires were collected from 484 residents in Daegu and Gyeongbuk. The questionnaires contained questions on the recognition of jeungpyun, purchasing, frequency, purchasing factor, and preferences for and purchasing of jeungpyun containing different kinds of makgeoilli. According to the survey, females were higher than males (31.4%) by 69.7%, and that for ages 20~30' was the highest. Most of them were students (39.7%), and 81.7% of them had an income of under 4,000,000 won per month. The results of the eating frequency rate showed that over half of the people had jeungpyun once a month, and the recognition of jeungpyun for females (3.63) was higher than that for males (3.09). The respondents who joyed eating jeungpyun mentioned 'good taste and texture' as the top reason for eating jeungpyun, and 'different food and bad flavor' was mentioned as the reason for disliking it. As a result, popularization of jeungpyun was based on two factors: 1) fundamental factor and 2) external factor, and both significantly influence the preference for and purchasing of jeungpyun,-. Therefore, future studies should be conducted to improve preference for jeungpyun as a functional food. The results of preference and purchasing show that jeungpyun prepared with rice, rubus coreanum miquel, black bean, grape and green mume makgeoilli.

• Korean Journal of Soil Science and Fertilizer
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• v.20 no.3
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• pp.261-268
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• 1987

A green house experiment was conducted to find out the differences in the amount of biologically fixed nitrogen and kjeldahl nitrogen on the different soil texture, kinds and amounts of fertilizer nitrogen under light (photosynthetic $N_2$-fixation) and dark (heterotrophic $N_2$-fixation) condition in submerged paddy soil. The reults obtained were summarized as follows; 1. The amount of biologically fixed nitrogen per mg carbon from different organic matter was obtained as 0.13 mg in glucose, 0.09 mg in rice straw, and 0.07 mg in refused mushroom compost and barley straw under 60 days of incubation. 2. Nitrogen fixing activities were decreased with increase of fertilizer nitrogen and those tendency was pronounced more in sandy soil with application of urea than that of ammonium sulfate. 3. The application of ammonium sulfate in sandy soil under light condition was increased the photosynthetic $N_2$-fixation and the applied urea was remarkably reduced the heterotrophic $N_2$-fixation in sandy soil. The proportion of biologically fixed total nitrogen after experiment in sandy soil was obtained as 25% for dark(heterotrophic $N_2$-fixation) and 75% for light (photosynthetic $N_2$-fixation) condition. On the other hand, very similar biological $N_2$-fixing tendency was obtained between kinds of nitrogen fertilizer and two light condition in clayey soil. 4. The kjeldahl nitrogen was remarkably decreased after experiment under dark condition with application of urea than that of light condition with ammonium sulfate, and no remarkable decreasing tendency was obtained in clayey soil between two kinds of fertilizer nitrogen. 5. The high significant positive correlationship was obtained between calculated biological nitrogen fixation by acetylene reducing activity and kjeldahl nitrogen after experiment under light (y=0.8488X-5.9632, $r=0.9928^{**}$, n=21) and dark (y=0.8795X-7.1056, $r=0.9782^{**}$, n=21) condition. In this experiment condition, conversion factors of 6:1 was obtained from biological nitrogen fixation to soil nitrogen.

• Journal of Food Hygiene and Safety
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• v.32 no.4
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• pp.298-305
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• 2017

Benzovindiflupyr is a new pyrazole carboxamide fungicide that inhibits succinate dehydrogenase of mitochondrial respiratory chain. This study was carried out to develop an analytical method for the determination of benzovindiflupyr residues in agricultural commodities using LC-MS/MS. The benzovindiflupyr residues in samples were extracted by using acetonitrile, partitioned with dichloromethane, and then purified with silica solid phase extraction (SPE) cartridge. Correlation coefficient ($r^2$) of benzovindiflupyr standard solution was 0.99 over the calibration ranges ($0.001{\sim}0.5{\mu}g/mL$). Recovery tests were conducted on 5 representative agricultural commodities (mandarin, green pepper, potato, soybean, and hulled rice) to validate the analytical method. The recoveries ranged from 79.3% to 110.0% and then relative standard deviation (RSD) was less than 9.1%. Also the limit of detection (LOD) and limit of quantification (LOQ) were 0.0005 and 0.005 mg/kg, respectively. The recoveries of interlaboratory validation ranged from 83.4% to 117.3% and the coefficient of variation (CV) was 9.0%. All results were followed with Codex guideline (CAC/GL 40) and Ministry of Food and Safety guideline (MFDS, 2016). The proposed new analytical method proved to be accurate, effective, and sensitive for benzovindiflupyr determination and would be used as an official analytical method.