• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 2001년도 Proceedings of 2001 International Symposium
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• pp.77-80
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• 2001

A variety of different methods to generate diverse proteins, including random mutagenesis and recombination, are currently available, and most of them accumulate the mutations on the target gene of a protein, whose sequence space remains unchanged. On the other hand, a pool of diverse genes, which is generated by random insertions, deletions, and exchange of the homologous domains with different lengths in the target gene, would present the protein lineages resulting in new fitness landscapes. Here we report a method to generate a pool of protein variants with different sequence spaces by employing green fluorescent protein (GFP) as a model protein. This process, designated functional salvage screen (FSS), comprises the following procedures： a defective GFP template expressing no fluorescence is firstly constructed by genetically disrupting a predetermined region(s) of the protein, and a library of GFP variants is generated from the defective template by incorporating the randomly fragmented genomic DNA from E. coli into the defined region(s) of the target gene, followed by screening of the functionally salvaged, fluorescence-emitting GFPs. Two approaches, sequence-directed and PCR-coupled methods, were attempted to generate the library of GFP variants with new sequences derived from the genomic segments of E. coli. The functionally salvaged GFPs were selected and analyzed in terms of the sequence space and functional property. The results demonstrate that the functional salvage process not only can be a simple and effective method to create protein lineages with new sequence spaces, but also can be useful in elucidating the involvement of a specific region(s) or domain(s) in the structure and function of protein.

• 한국농공학회논문집
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• 제62권1호
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• pp.17-27
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• 2020

The objective of this study was to assess the flood probability based on temporal distribution of forecasted-rainfall in Cheongmicheon watershed. In this study, 6-hr rainfalls were disaggregated into hourly rainfall using the Multiplicative Random Cascade (MRC) model, which is a stochastic rainfall time disaggregation model and it was repeated 100 times to make 100 rainfalls for each storm event. The watershed runoff was estimated using the Clark unit hydrograph method with disaggregated rainfall and watershed characteristics. Using the peak discharges of the simulated hydrographs, the probability distribution was determined and parameters were estimated. Using the parameters, the probability density function is shown and the flood probability is calculated by comparing with the design flood of Cheongmicheon watershed. The flood probability results differed for various values of rainfall and rainfall duration. In addition, the flood probability calculated in this study was compared with the actual flood damage in Cheongmicheon watershed (R² = 0.7). Further, this study results could be used for flood forecasting.

• 한국재료학회지
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• 제22권7호
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• pp.329-334
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• 2012

Two types of nanoclusters, termed Cluster (1) and Cluster (2) here, both play an important role in the age-hardening behavior in Al-Mg-Si alloys. Small amounts of additions of Cu and Ag affect the formation of nanoclusters. Two exothermic peaks were clearly detected in differential scanning calorimetry(DSC) curves by means of peak separation by the Gaussian method in the base, Cu-added, Ag-added and Cu-Ag-added Al-Mg-Si alloys. The formation of nanoclusters in the initial stage of natural aging was suppressed in the Ag-added and Cu-Ag-added alloys, while the formation of nanoclusters was enhanced at an aging time longer than 259.2 ks(3 days) of natural aging with the addition Cu and Ag. The formation of nanoclusters while aging at $100^{\circ}C$ was accelerated in the Cu-added, Ag-added and Cu-Ag-added alloys due to the attractive interaction between the Cu and Ag atoms and the Mg atoms. The influence of additions of Cu and Ag on the clustering behavior during low-temperature aging was well characterized based on the interaction energies among solute atoms and on vacancies derived from the first-principle calculation of the full-potential Korrinaga-Kohn-Rostoker(FPKKR)-Green function method. The effects of low Cu and Ag additions on the formation of nanoclusters were also discussed based on the age-hardening phenomena.

• 대한조선학회논문집
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• 제38권3호
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• pp.41-46
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• 2001

최근에 선박이 대형화되는 추세에 힘입어 조선소는 광폭천흘수선, 초대형 원유운반선 및 초대형 컨테이너선 등을 건조하고 있다. 이와 같은 선박은 상대적으로 다른 선박에 비해 강성이 작기 때문에 파랑 중에서 유탄성 운동을 하게 되고, 입사하는 파고가 작은 경우에도 선체의 2절 모드의 진동에 의해 선체의 갑판이 피로 파괴되는 경우가 종종 발생하는 것으로 알려져 있다. 본 논문에서 전진하는 선박의 유체 압력을 계산하기 위해 적분방정식은 3차원 소오스 분포법을 사용하고, 그린함수는 전진하면서 동요하는 형태를 이용하였다. 방사문제는 선박을 여러 개의 단면으로 나누어 단면간의 간섭효과를 고려하여 heave 및 pitch 강제동요와 관련된 부가질량 및 조파 감쇠계수를 계산하였고, 파강제력은 각 단면에서 선행해에 의한 힘만 고려하였다. 선박의 각 단면의 수직운동은 선박에 대한 운동방정식을 이용하고 강성행렬은 오일러 보 이론에 의해 산정되었다. 계산은 Esso-Osaka 선박을 모델로 도입하여 입사하는 파도의 주파수가 변함에 따른 선박의 각 단면에 대한 운동, 굽힘 모우멘트를 계산하였다.

• Geomechanics and Engineering
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• 제19권1호
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• pp.11-20
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• 2019

The use of electrokinetic dissipation method to study the fluid flow law in micro-pores is of great significance to reservoir rock microfluidics. In this paper, the micro-capillary theory was combined with the coupling model of the seepage field and the current field under the excitation of the harmonic signal, and the coupling theory of the electrokinetic effect under the first-order approximation condition was derived. The dissipation equation of electrokinetic dissipation and viscous resistance dissipation and its solution were established by using Green's function method. The physical and mathematical models for the electrokinetic dissipation of reservoir rocks were constructed. The microscopic mechanism of the electrokinetic dissipation of reservoir rock were theoretically clarified. The influencing factors of the electrokinetic dissipation frequency of the reservoir rock were analyzed quantitatively. The results show that the electrokinetic effect transforms the fluid flow profile in the pores of the reservoir from parabolic to wavy; under low-frequency conditions, the apparent viscosity coefficient is greater that one and is basically unchanged. The apparent viscosity coefficient gradually approaches 1 as the frequency increases further. The viscous resistance dissipation is two orders of magnitude higher than the electrokinetic effect dissipation. When the concentration of the electrolyte exceeds 0.1mol/L, the electrokinetic dissipation can be neglected, while for the electrolyte solution (<$10^{-2}M$) in low concentration, the electrokinetic dissipation is very significant and cannot be ignored.

• 반도체디스플레이기술학회지
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• 제20권4호
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• pp.89-95
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• 2021

As the influence of fine dust on society spreads gradually, the public's interest in indoor air is increasingly rising. Air-purifying plants are drawing keen attention due to their natural purifying function enabled by plant physiology. However, as their fine dust reduction mechanism is limited to adsorption only, vegetation bio-filters that optimize purification effects through integration with air-conditioning systems is rising as an alternative. In accordance with the relevant standard test methods, this study looked into the fine dust reduction assessment method by air-conditioning airflow volume that can be used for the industrial spread of vegetation bio-filters. In the case of PM₁₀ at 300 ㎍/m³, it was in the order of EG-B(3,500CMH, 29 min.) < EG-A (2,500CMH, 37 min.) < CG(0CMH, 64 min.) for reaching the maintenance level (100 ㎍/m³) of publicly used facilities. For reaching the WHO Guideline(50 ㎍/m³) requirement, it was in the order of EG-B (51 min.) < EG-A (160 min.) < CG (170 min.). In the case of PM_2.5, it was in the order of EG-B (26 min.) < EG-A (33 min.) < CG (57 min.) for reaching the maintenance level (50 ㎍/m³) of publicly used facilities. It was in the order of EG-B (48 min) < EG-A (140 min) < CG (158 min) for reaching the WHO Guideline (25 ㎍/m³) requirement. The findings from the analysis showed that fine dust can be reduced most efficiently when the system is operated at 3,500CMH level. The limitation of this study is that due to the absence of a way of assessing the stress of plants in vegetation bio-filters, generating optimal air-conditioning air flow of the relevant system and economics analysis against the existing facility-type air purification system have been clarified, which should be explored further though follow-up studies.

• Advances in nano research
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• 제15권3호
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• pp.263-275
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• 2023

The electron transport properties of Y-type zigzag branched carbon nanotubes (CNTs) are of great significance for micro and nano carbon-based electronic devices and their interconnection. Based on the semi-empirical method combining tight-binding density functional theory and non-equilibrium Green's function, the electron transport properties between the branches of Y-type zigzag branched CNT are studied. The results show that the drain-source current of semiconducting Y-type zigzag branched CNT (8, 0)-(4, 0)-(4, 0) is cut-off and not affected by the gate voltage in a bias voltage range [-0.5 V, 0.5 V]. The current presents a nonlinear change in a bias voltage range [-1.5 V, -0.5 V] and [0.5 V, 1.5 V]. The tangent slope of the current-voltage curve can be changed by the gate voltage to realize the regulation of the current. The regulation effect under negative bias voltage is more significant. For the larger diameter semiconducting Y-type zigzag branched CNT (10, 0)-(5, 0)-(5, 0), only the value of drain-source current increases due to the larger diameter. For metallic Y-type zigzag branched CNT (12, 0)-(6, 0)-(6, 0), the drain-source current presents a linear change in a bias voltage range [-1.5 V, 1.5 V] and is symmetrical about (0, 0). The slope of current-voltage line can be changed by the gate voltage to realize the regulation of the current. For three kinds of Y-type zigzag branched CNT with different diameters and different conductivity, the current-voltage curve trend changes from decline to rise when the branch of drain-source is exchanged. The current regulation effect of semiconducting Y-type zigzag branched CNT under negative bias voltage is also more significant.

• 한국염색가공학회지
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• 제35권4호
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• pp.239-245
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• 2023

Cellulose is an abundant biodegradable material in nature with excellent properties, but due to its poor processability, it has been widely studied for processing through modification. Cellulose acetate propionate (CAP) is a cellulose derivative in which the hydroxyl group of cellulose is replaced by acetyl and propionyl groups. CAP has several advantages, such as excellent solubility, structural stability, light and weather resistance, and good transparency. Porous nanofibers with excellent specific surface area, which can be applied in various fields, can be easily formed by the phase separation method using highly volatile solvents. High speed centrifugal spinning is a nano/micro fiber preparation method with advantages such as fast spinning and easy alignment control. In this study, a CAP/polybutylene succinate (PBS) spinning solution with chloroform as solvent was prepared to prepare porous microfibers and the fiber morphology was examined as a function of the disk rotation speed in an high speed centrifugal spinning device.

• 한국조경학회지
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• 제38권2호
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• pp.109-118
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• 2010

본 연구는 랜드스케이프 어바니즘을 적용한 도시재생적 실천전략을 모색하기 위해 연구되었다. 특히, 랜드스케이프 어바니즘을 산업사회를 넘어 지식정보산업사회의 도래와 함께 나타나는 도시화의 새로운 특성과 양상, 또 산업화시대의 신도시 이상을 넘어 도시재생이라는 새로운 도시 이상과의 상관성 맥락에서 도시재생에 어떻게 적용할 것인가의 관점에서 수행되었다. 본 연구의 결과, 랜드스케이프 어바니즘에서의 랜드스케이프의 의미가 도시를 대상으로 시각적, 장식적 차원의 정태적 풍경이 아니라, 변화, 진화 등의 맥락에서 동태적 과정을 대상으로 하고 있고, 지식정보산업사회가 지식 정보의 흐름 등 가속적 변화와 과정적 특징을 보이며, 이러한 사회적 특징에 따라 도시화도 형태와 기능 중심에서 흐름, 네트워킹, 중심성과 결절성, 지표면 등의 개념으로 구성되는 가치지향의 과정적, 동태적 특성을 보인다는 점에서 이들 3자간에는 그 본질적 개념에 있어 상호 관통되고 소통되고 있는 것으로 나타났다. 특히, 산업화시대의 도시화에 대한 반성으로서 21세기 새로운 도시이상으로서 대두되고 있는 도시재생은 본질적으로 아스팔트와 콘크리트로 점유된 도시 경관판을 그린 인프라 구축과 디자인이라는 맥락에서 생태판에 문화를 도입한 문화판으로 구축해야 한다는 당위성에서 랜드스케이프 어바니즘과 일정의 괘를 같이 하고 있었다. 본 연구에서 제시된 랜드스케이프 어바니즘을 적용한 8개의 도시재생전략으로는 도시가 자연과 역동적으로 소통하고 진화할 수 있는 생태판의 구축, 생태와 도시민과의 소통을 위한 생태판의 문화판으로의 전환, 주택 및 상업지역에서의 그린 인프라 구축 등의 관점에서, 건폐율 규제, 높이 규제 완화 등의 필요성이 제시 되었다. 또한, 도시이전적지의 공원화, 하천 등 도시워터프론트의 공원화 등의 전략에서 공원의 도시적 관입 및 확산과 더불어 공원혼합용도에 대한 도입이 논의되었다. 이러한 새로운 경관판을 형성하기 위한 실행전략으로 사회의 변화에 역동적으로 부응할 수 있는 전략계획제의 도입이 논의되었고, 특히, 이미지니어링을 통한 가치계획을 추구함으로써 기능중심의 도시화에서 가치창출과 브랜드형성 차원의 도시화로 진화시켜 나아가야 할 것을 제시하였다. 이 모든 전략을 위해 기존의 경관, 토지이용 등 건축 및 도시개발 관련 각종 제도가 개선되어야 할 것으로 제안되었다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제7권5호
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• pp.303-310
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• 2002

Cell therapy applied to wound healing or tissue regeneration presents a revolutionary realm to which principles of gene engineering and delivery may be applied. One promising application is the transplantation of cells into the wounded tissue to help the tissue repair. However, when cells are transplanted from in vitro to in vivo, immune rejection occurs due to the immune response triggered by the activation of T-cell, and the transplanted cells are destroyed by the attack of activated T-cell and lose their function. Immune suppressant such as FK506 is commonly used to suppress immune rejection during transplantation. However, such kind of immune suppressants not only suppresses immune rejection in the periphery of transplanted cells but also suppresses whole immune response system against pathogenic infection. In order to solve this problem, we developed a method to protect the desired cells from immune rejection without impairing whole immune system during cell transplantation. Previously, we reported the success of constructing glomerular epithelial cells for removal of immune complex, in which complement receptor of type 1 (CR1) was over-expressed on the membrane of renal glomerular epithelial cells and could bind immune complex of DNA/anti-DNA-antibody to remove immune complex through phagocy-tosis [1]. Attempting to apply the CR1-expressing cells to cell therapy and evade immune rejection during cell transplantation, we constructed three plasmids containing genes encoding a soluble fusion protein of cytolytic T lymphocyte associated antigen-4 (CTLA4Ig) and an enhanced green fluorescent protein (EGFP). The plasmids were transfected to the above-mentioned glomerular epithelial cells to express both genes simultaneously. Using the clone cells for cell transplantation showed that mice with autoimmune disease prolonged their life significantly as compared with the control mice, and two injections of the cells at the beginning of two weeks resulted in remarkable survivability, whereas it requires half a year and 50 administrations of proteins purified from the same amount of cells to achieve the same effect.