• BMB Reports
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• 제40권2호
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• pp.189-195
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• 2007

Although the incidence and severity of atopic dermatitis (AD) is steadily increasing at an alarming rate, its pathogenic mechanisms remain poorly understood yet. Recently, we found that the expression of Grb7 protein was markedly decreased in AD patients using proteomic analysis. In the present study, human Grb7 gene was fused with PEP-1 peptide in a bacterial expression vector to produce a genetic in-frame PEP-1-Grb7 fusion protein. The expressed and purified PEP-1-Grb7 fusion proteins transduced efficiently into skin cells in a time- and dose-dependent manner when added exogenously in culture media. Once inside the cells, the transduced PEP-1-Grb7 protein was stable for 48 h. In addition, transduced PEP-1-Grb7 fusion protein markedly increased cell viability in macrophage RAW 264.7 cells treated with LPS by inhibition of the COX-2 expression level. These results suggest that the PEP-1-Grb7 fusion protein can be used in protein therapy for inflammatory skin disorders, including AD.

• 천문학회보
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• 제41권2호
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• pp.63.1-63.1
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• 2016

We observed Ultra-Long GRB 111209A using NASA's 3m InfraRed Telescope Facility (IRTF). The observation was started around 40 min later than T0 = 07:12:08 UT of Swift's BAT, lasted for 24 min. The spectrum was extracted using Spextool package. The NIR SEDs show power law distribution indicating afterglow emission from the GRB according to the fireball model with beta ~ 1.2. Also they do not show thermal emission component compared to the SED of "Christmas burst" GRB 101225A. Because there is no other NIR data with this observation epoch, this data can be compared only with TAROT-R band. It seems NIR data has the same flare which exists in R band as an optical flare.

• 천문학회보
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• 제37권2호
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• pp.82.1-82.1
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• 2012

GRB100205A is a Gamma Ray Burst (GRB) which is suspected to be at redshift z=11-13 due to its very red H-K color($(H-K)_{vega}=2.1{\pm}0.5$). We observed a field centered at GRB100205A with Wide Field Camera (WFCAM) at United Kingdom Infrared Telescope (UKIRT) in Hawaii, in order to search a quasar that could be located around the GRB. The images were obtained in J, H, and K filters covering a square area of $0.78deg^2$. Our J-, H-, and K-band data reach the depths of 22.5, 22.1, and 21.0 mag (Vega) at $5{\sigma}$, respectively. Also using z-band image observed by CFHT, we find 8 candidates that have colors consistent with a quasar at z=11-13(non-detection in z-, J-band and $(H-K)_{vega}$ > 1.6). However, the shallow depths of J-, H-band are not enough to verify their true nature. Instead, we identify many red objects to be old or dusty galaxies at $z{\geq}3$. The number density of such objects appears about twice or more than that of the field of Cosmological Evolution Survey (COSMOS) and Ultra Deep Survey (UDS) of UKIRT Infrared deep sky survey (UKIDSS). On scales between 0.18' and 15' the correlation function is well described by a power law with an exponent of ${\approx}-0.9$ and this implies that those objects are like galaxies. It is interesting that many red galaxies exist in the region where the GRB was detected.

• Mass Spectrometry Letters
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• 제12권1호
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• pp.16-20
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• 2021

We aimed to compare the content of ginsenosides and the pharmacokinetics after the oral administration of four different ginseng products at a dose of 1 g/kg in rats. The four different ginseng products were fresh ginseng extract, red ginseng extract, white ginseng extract, and saponin enriched white ginseng extract prepared from the radix of Panax ginseng C.A. Meyer. The ginsenoside concentrations in the ginseng product and the rat plasma samples were determined using a liquid chromatography-tandem mass spectrometry (LC-MS/MS). Eight or nine ginsenosides of the 15 tested ginsenosides were detected; however, the content and total ginsenosides varied depending on the preparation method. Moreover, the content of triglycosylated ginsenosides was higher than that of diglycosylated ginsenosides, and deglycosylated ginsenosides were not present in any preparation. After the single oral administrations of four different ginseng products in rats, only four ginsenosides, such as 20(S)-ginsenosides Rb1 (GRb1), GRb2, GRc, and GRd, were detected in the rat plasma samples among the 15 ginsenosides tested. The plasma concentrations of GRb1, GRb2, GRc, and GRd were different depends on the preparation method but pharmacokinetic features of the four ginseng products were similar. In conclusion, a good correlation between the area under the concentration curve and the content of GRb1, GRb2, and GRc, but not GRd, in the ginseng products was identified and it might be the result of their higher content and intestinal biotransformation of the ginseng product.

• 천문학회보
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• 제37권2호
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• pp.66-66
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• 2012

We describe the space project of Ultra-Fast Flash Observatory (UFFO), which will observe early optical photons from gamma-ray bursts (GRBs) with a sub-second optical response, for the first time. The UFFO will probe the early optical rise of GRBs, opening a completely new frontier in GRB and transient studies, using a fast-response rotatable mirror system which redirects opitical path to telescope instead of slewing of telescopes or spacecraft. In our small UFFO-Pathfinder experiment, scheduled to launch aboard the Lomonosov satellite in June 2012, we use a motorized mirror in our Slewing Mirror Telescope instrument to achieve less than one second optical response after X-ray trigger. We describe the science and the mission of the UFFO project, including a serious version called UFFO-100 which will be launched in 2014. With our program of ultra-fast optical response GRB observatories, we aim to gain a deeper understanding of GRB mechanisms, and potentially open up the z>10 universe to study via GRB as point source emission probes.

• 생약학회지
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• 제30권2호
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• pp.202-206
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• 1999

The MeOH extracts obtained from the leaves of Eucommia ulmoides were solvent-fractionated with EtOAc, n-BuOH, and $H_20$, successively. From the EtOAc extract showing Grab2-Shc inhibitory activity, a flavonol and a lignan compounds were isolated through repeated silica gel column chromatographies. By interpretation of several spectral data and adaptation of acetylation method, the chemical structures of the compounds were determined as 5,7,3',4'-tetrahydroxy-flavonol (quercetin), and 4,4',8',9-tetrahydroxy-3,3'-dimethoxy-7,9'-cyclolignan {(-)-olivil)}, respectively. The compounds exhibited $IC_{50}$ values in Grb2-Shc inhibitory activity to be 93 and $210\;{\mu}mole/l$, respectively.

• Genomics & Informatics
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• 제6권1호
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• pp.32-35
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• 2008

Little evidence supports the existence of imprinted genes in chicken. Imprinted genes are thought to be intimately connected with the acquisition of parental resources in mammals; thus, the predicted lack of this type of gene in chicken is not surprising, given that they leave their offspring to their own heritance after conception. In this study, we identified several imprinted genes and their orthologs in human, mouse, and zebrafish, including 30 previously identified human and mouse imprinted genes. Next, using the HomoloGene database, we identified six orthologous genes in human, mouse, and chicken; however, no orthologs were identified for SLC22A18, and mouse Ppp1r9a was not included in the HomoloGene database. Thus, from our analysis, four candidate chicken imprinted genes (IGF2, UBE3A, PHLDA2, and GRB10) were identified. To expand our analysis, zebrafish was included, but no probe ID for UBE3A exists in this species. Thus, ultimately, three candidate imprinted genes (IGF2, PHLDA2, and GRB10) in chicken were identified. GRB10 was not significant in chicken and zebrafish based on the Wilcoxon-Mann-Whitney test, whereas a weak correlation between PHLDA2 in chicken and human was identified from the Spearman's rank correlation coefficient. Significant associations between human, mouse, chicken, and zebrafish were found for IGF2 and GRB10 using the Friedman's test. Based on our results, IGF2, PHLDA2, and GRB10 are candidate imprinted genes in chicken. Importantly, the strongest candidate was PHLDA2.

• 고려인삼학회:학술대회논문집
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• 고려인삼학회 1988년도 학술대회지
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• pp.92-98
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• 1988

본 연구는 중앙대서전에 인삼의 주 효과중 건망증 방지작용이라고 수록되어 있는 것을 확인하기 위하여 행하여졌다. Step through 시험에서 $GRb_1$과 $GRg_1$은 기억력 획득을 용이하게 하였으며 전기충격 쇼크에 의해 야기되는 기억상실 효과를 억제하였다. 게대가 $Rg_1$은 에타놀 야기 기억재생 저해작용을 방지하였다. Stepdown test에 있어서 $Rb_1,\;Rb_2,\;Rg_1$은 전기충격쇼크에 의해 야기되는 기억보지 저해효과를 방지하였다. 또한 $Rg_1$은 에타놀에 의해 야기되는 기억재생 저해효과를 방지하였으며 단기적 기억력 획득을 용이케 하였다. Shuttle box와 lever press 시험에서도 $Rb_1$을 제외하고 나머지는 기억획득과 재생에 효과를 미치지 못했다. $Rb_1$은 shuttle box 시험에서 조건회피반응의 재생 (retrieval)을 억제하였다. 이와 같은 4가지 시험이 끝난후에 인삼의 구강투여가 진정, 진통, 해열, 진경효과와 자발 및 탐색활동에 미치는 영향을 500 mg/kg 투여 범위내에서 조사하였으나 아무런 반응도 관찰되지 않았다. 현재까지 연구결과는 $Rg_1$이 기억의 재생 및 학습반응의 획득과정에 대하여 효과가 있다는 것을 의미한다. 최근의 신경성장인자(NGF)가 성숙동물에 있어 뇌신경세포의 생존, 재생 및 조절에 미치는 작용에 관한 연구결과는 지능장해와 건망증에 대한 신경성장인자의 중요성을 시사했다. NGF에 의해 야기된 신경세포돌기 성장의 특이성에 관한 연구결과를 병아리 배배근 신경절에 있어서 NGF의 영향은 $Rh_1$에 의해 증가되었다. 다음으로 $Rb_1$은 병아리 배의 감각 및 교감신경단위에 있어서 NGF-관련 신경섬유 증가를 강화시켰다. NGF와 $Rb_1$을 함께 했을 때도 역시 쥐 대뇌피질의 신경세포 생존수를 증가시키는 경향을 보였다. NGF는 쥐의 배격부 부근의 신경세포를 배양했을 때 cholineacetyl transferase 활성을 증가시키지 않았다. 이러한 결과로 $Rb_1$은 뇌에 있어서 신경세포의 생존이나 재생에 중요한 역활을 한다는 것은 알 수 있었다.

• 천문학회보
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• 제43권1호
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• pp.39.2-39.2
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• 2018

GW170817/GRB170817A establishes a double neutron star merger as the progenitor of a short gamma-ray burst, starting 1.7 s post-coalescence. GRB170817A represents prompt or continuous emission from a newly formed hyper-massive neutron star or black hole. We report on a deep search for broadband extended gravitational-wave emission in spectrograms up to 700 Hz of LIGO O2 data covering this event produced by butterfly filtering comprising a bank of templates of 0.5 s. A detailed discussion is given of signal-to-noise ratios in image analysis of spectrograms and confidence levels of candidate features. This new pipeline is realized by heterogeneous computing with modern graphics processor units (GPUs). (Based on van Putten, M.H.PM., 2017, PTEP, 093F01.)

• 천문학논총
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• 제32권1호
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• pp.113-116
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• 2017

A significant number of the parameters of a gamma-ray burst (GRB) and its host galaxy are calculated from the afterglow. There are various methods obtaining extinction values for the necessary correction for galactic foreground. These are: galaxy counts, from HI 21 cm surveys, from spectroscopic measurements and colors of nearby Galactic stars, or using extinction maps calculated from infrared surveys towards the GRB. We demonstrate that AKARI Far-Infrared Surveyor sky surface brightness maps are useful uncovering the fine structure of the galactic foreground of GRBs. Galactic cirrus structures of a number of GRBs are calculated with a 2 arcminute resolution, and the results are compared to that of other methods.