• The Plant Pathology Journal
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• 제19권5호
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• pp.235-238
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• 2003

The resistance to crown gall in grape rootstocks was evaluated by inoculating cuttings from 27 grape rootstock varieties with Agrobacterium vitis strain Cheonan 493. Thmors were formed in all varieties of grape rootstocks tested in this study. The symptoms were observed in the stems of all plants tested including '196-17' and '41B'. Based on the measurement of tumor weight on the stems of grape rootstocks, '779P' was found to be higbly susceptible to crown gall. While some varieties such as 'Gloire', '140R', '101-14M', '3309C', and '333EM' were found to be relatively resistance to crown gall. Varieties such as '99R', '1447P', 'Rupestris du lot', 'll0R', 'Freedom', and '41B' were also found to be susceptibl~ to crown gall. Other~ varieties including 'l103P', 'Teleki. 5C', '420A', 'Golia', and '5BB' were found to be moderately susceptible.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.70.2-70
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• 2003

To evaluate the resistance to crown gall in grape rootstocks, cuttings from twenty seven grape rootstocks were inoculated with Agrobacterium vitis Cheonan 493 and size of galls from grapevines was measured in a greenhouse. Tumors were formed in all varieties of grape rootstocks tested in this study and no grape rootstock variety was immune to crown gall. Tumors were found on the stems of all plants tested in '196-17'and '41B' Based on measuring size and weight of galls formedon the stem of grape rootstocks, '779P' was extremely susceptible to crown gall. Some varieties such as 'Gloire', '140R', '101-l4M', '3309C', and '333EM' found to be resistant, while '99R', '1447P', 'Rupestris du lot', '110R', 'Freedom', and '41B'were susceptible and '1103P', '5C', '420A', 'Golia', and '5BB' were moderately susceptible to crown gall.

• Journal of Plant Biotechnology
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• 제35권2호
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• pp.127-132
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• 2008

In bioreactor cultures of plants, inoculum density is an important factor affecting growth and proliferation of the plantlets. To maximize shoot growth and proliferation of grape rootstock '5BB' in bioreactors, inoculum density varied at 15, 30, 45 and 60 single nodes in a 3-liter scale balloon type bioreactor, respectively and cultured for 40 days. Results suggested that the growth and the photosynthesis of the plantlet were greatly affected by inoculum density in the bioreactor. The inoculum density of 45 nodes resulted in the greatest growth (910.4 mg/shoot FW, 764.4 mg/root FW) followed by 30 nodes. $CO_2$ assimilation rate, stomatal conductance, transpiration rate of the plantlet were also highest at the inoculum density of 45 nodes. Significant reduces in shoot and root growth (426.5 mg/shoot FW, 248.4 mg/root FW) were observed at the inoculum density of 60 nodes. When the inoculum density decreased by 15 nodes, plantlets were malformed due to hyperhydricity, resulting in the highest transpiration rate and the lowest $CO_2$ assimilation rate. The plantlets stressed by the inoculum density at 15 nodes and 60 nodes showed larger number and irregular shape of stomata compared to the plantlets inoculated with 45 nodes.

• 식물병연구
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• 제13권2호
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• pp.98-103
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• 2007

포도나무의 추위피해는 여러 형태로 나타난다. 월동피해 중에는 기계적 피해, 동해 또는 수세의 약화 원인이 된다. 본 연구는 '거봉'의 뿌리혹병의 피해를 억제하기 위하여 저항성 대목의 선발과 월동법 개선을 통한 방제법을 개발하기 위하여 실시하였다. SO4, 5BB, 3306대목에 $10^4cfu/ml$의 뿌리혹병균(YK2823, YK3312, LMG259, HKA234)을 접종했을 때는 혹이 발병하지 않았으나 $10^6cfu/ml$의 높은 농도에서는 모든 대목에서 혹이 형성된다. 그러나 혹의 크기는 거봉에서보다 매우 작았다. 인공접종에 의하여 3품종의 뿌리혹병 저항성 대목을 선발하였다. 볏짚과 보온덮개로 줄기와 가지에 피복하는 월동법은 뿌리 혹병을 예방하는데 가장 효과적이었다. 이러한 처리방법은 포도나무가 월동하는 동안 수체의 온도 차이가 $9.6^{\circ}C$로 온도변화가 최소화되었고 동해발생이 없어 정상적으로 생육하였다.