• Applied Microscopy
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• v.35 no.4
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• pp.91-97
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• 2005

The acinar cells and secretory granules of the parotid salivary gland were examined in the lesser white toothed shrew, Crocidura suaveolens and the big white-toothed shrew, C. lasiura. The parotid gland of both species were a serous gland having only one kind of serous acinar cells, and had conventional arrangement of acini and intercalated, granular and striated ducts. In case of C. suaveolens, serous acinar cells had well developed rER, prominent Golgi complex, several large mitochondria and abundant moderate dense secretory granules with various stages of the maturing or fusing process. Immature acinar secretory granules were only or mainly filled with fine strong dense specks and had an indistinct limiting membrane, and mature granules were filled with homogeneous pale large round center and had fine strong dense specks at the periphery of the homogeneous pale center and a distinct limiting membrane. In case of C. lasiula, serous acinar cells had well developed rER, prominent Golgi complex, several large mitochondria and abundant dense secretory granules with maturing or fusing process. Immature acinar secretory granules were only filled with pale rough specks and had an indistinct limiting membrane, and mature granules were only filled with rough dense specks and had a distinct limiting membrane. Eventually The acinar secretory granules of C. suaveolens were seen moderate at the light and ultrastuctural level, those of C. lasiura were strong dense at the light microscopic level and dense at the ultrastructural level.

• Journal of Sericultural and Entomological Science
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• v.16 no.2
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• pp.119-125
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• 1974

When the nomal silkworms reached active time of 3rd instar stage both non-moulting larva and normal silkworms from the same rearing tray were collected and fixed. The silkworms in 4th instar stage whose growth was as dwarfish as those in 1st and 2nd instar stages were also collected and fix with the normal silkworms. Non-moulting larva and normal silkworms were morphologically compared and the examined results from the tissue inspection are summarized as follows： 1. In spite of the fact that the normal silkworms reached the active eating time of 3rd instar stage non-moulting silkworms were dwarfish as if they had been reared for two days. Non-moulting silkworms which were observed at the time of 4th instar stage showed no much difference in their growth. 2. There was the tendency that the exuvial gland as was shown in Fig. 1 and 2 was flat cyslidium of ellipse and its size at thorax was small shile the gland at abdomen was big. 3. The exuvial gland at thorax has been reported to be bigger at thoracic base than at dorsal vessel but according to the present it was examined to be irregular. 4. The size of exuvial gland of silkworms in the active eating stage of 3rd instar was from 151.3${\mu}$ (major axis) to 94.5${\mu}$ (minor axis) at prothorax and from 568.6${\mu}$ (major axis) to 495.1${\mu}$ (minor axis) at 7th abdominal segment. The sire oe exuvial gland of non-moulting silkworm was 57.5${\mu}$ (major axis) to 51.3${\mu}$ (minor axis) at prothorax and from 91.5${\mu}$ (major axis) to 75.5${\mu}$ (minor axis) at 5th abdominal segment (see Fig. 1) 5. When the normal silkworms reached 4th instar active eating stage its exuvial gland was compared to that of dwarfish silkworm. The result was that the size of normal silkworm at prothorax was from 252.2${\mu}$ (major axis) to 131.6${\mu}$ (minor axis) and the size of exuvial gland at 7th abdominal segment was from 691.5${\mu}$ (major axis) to 493.4${\mu}$ (minor axis) while the sire of exuvial gland of non-moulting at prothorax was from 71.4${\mu}$ (major axis) to 61.5${\mu}$ (minor axis) and the size of the non-moulting silkworm's 8th abdominal segment was from 94.6${\mu}$ (major axis) to 71.5${\mu}$ (minor axis) (See Table 2) 6. There was a remarkable difference in the from of exuvial gland of non-moulting silkworm. The size of alveolar of the non-moulting silkworm was many times larger compared to that of normal silkworm 7. There was no great difference between secretory cells of normal and non-moulting silkworms but the granular type exuvial gland was small in sire compared to that of normal silkworm.

• The Korean Journal of Cytopathology
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• v.8 no.1
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• pp.52-56
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• 1997

Oxyphilic (Hurthle) cells have abundant eosinophilic granular cytoplasms and occur in both benign and malignant neoplasms of the thyroid gland. Most published studies described mainly oxyphilic tumors of follicular type, and reports on oxyphilic papillary thyroid carcinomas are rare. The oxyphilic variant of papillary carcinoma differs from the classic papillary carcinoma in its more aggressive biological behavior. We report a case of oxyphilic papillary carcinoma exhibiting exclusively oxyphilic cells in a follicular pattern without papillary structures. The pattern of papillary carcinoma was confirmed by fine needle aspiration material and lymph nodal metastasis, both of which revealed typicaled papillary structures with characteristic nuclear features, psammoma bodies, and oxyphilic cytoplasm.

• The Korean Journal of Zoology
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• v.39 no.2
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• pp.223-230
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• 1996

Fine structure of the neuromuscular junction in the venomous organ of the spider, Agelena li'mbutq, was studied using high magnification electron microscope. The motor nerve endings at neuromuscular contact area composed of neurons and neuroslial cells were located between musculature and extracellular sheath of the venom gBand. At the synaptic contact between a motor axon and a muscle fiber in the musculature, spherical synaptic vesicles were prominent in the nerve terminal. The sarcoplasm beneath the neuromuscular synapse has a granular appearance and lacks mvofilaments. And the main axon gives off a branch between the muscle fibers. The synaptic regions of this organ are located close to the myofilaments unlike to other chelicerate classes. Moreover the postsvnaptic complex of vesicles and membrane invasinations present in other synaptic legions are absent from these legions in this venomous organ.

• Applied Microscopy
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• v.22 no.2
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• pp.1-14
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• 1992

This experiment was performed to study the morphological responses of the parafollicular cells of rat following X-ray irradiation. Male rats were divided into normal and experimental groups. The head and neck region of the rat, under sodium thiopental anesthesia, was exposed to 3,000 rads or 6,000 rads of radiation in a single dose, respectively. The source was a Mitsubishi Linear Accelerator ML-4MV. The target to skin distance was 80 cm, and the dose rate was 200 rads/min. The rate of experimental groups were sacrificed on the 6th hour, 2nd and 6th day after X-ray irradiation. Pieces of the tissue taken from the thyroid gland were fixed in 2.5% glutaraldehyde-1.5% paraformaldehyde (0.1M Millonig's phosphate buffer, pH 7.3), and in 1% osmium tetroxide (0.1M Millonig's phosphate buffer, pH 7.3), and embedded in araldite mixture. The ultrathin sections stained with uranyl acetate and lead citrate were observed with JEM 100 CX-II electron microscope. The results were as follow; 1. Two types of the parafollicular cells, according to their electron densities, were found, i. e., light cells and dark cells. 2. Three types of the parafollicular cells, according to their sizes of secretory granules were found, i.e., small granule cells ($85nm{\pm}20.1;64{\sim}102nm$), medium granule cells ($120nm{\pm}26.5;77{\sim}179nm$), and large granule cells ($165nm{\pm}25.7;128{\sim}189nm$). 3. The differential ultrastructural changes of the cells according to their cell types, i.e., dark and light cell, or small, medium and large granule cells, were hardly observed in the time and dose range covered by this study. 4. The morphological changes of the parafollicular cells were not pronounced after exposure to 3,000 rads of X-ray. 5. Swollen cisternae of the granular endoplasmic reticulum and partial cytolysis were observed after exposure to 6,000 rads of X-ray. 6. Above results suggest that the parafollicular cells showed the alterations of mitochondrial and granular endoplasmic reticular swelling, and partial cytolysis, but only in doses of 6,000 rads.

• Applied Microscopy
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• v.31 no.4
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• pp.325-331
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• 2001

Integumentary structures of the stone flounder, Karefus bicoloratus were examined by means of the light and transmission electron microscopy. Stratified epidermal layer consists of supporting cells, unicellular glands and granular cells. The epidermal layer could be classified into superficial, intermediated and basal layer by morphology and structure of the supporting cells . The cytoplasm of supporting cells is divided into cortex and medullar part. In the cortex microfilaments are well developed. Mucous cells of unicellular gland were observed in the superficial and intermediated layer of the epidermis. The mucous materials were identified as glycoprotein of neutral and carboxylated mucosubstance by histochemical methods. Club cell has well developed smooth endoplasmic reticula and Golgi complex in the cytoplasm. Granular cells were observed in the intermediated and basal layer, and the cytoplasm is occupied with membrane-bounded granules of electron dense. Three types of pigment cells could be distinguished with electron density of cytoplasmic inclusions. Nerve myelins were observed near the pigment cells.

• Applied Microscopy
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• v.34 no.2
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• pp.83-93
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• 2004

Integumentary structures of the rockfish, Sebastiscus tertius were examined by means of the light and transmission electron microscopy. Stratified epidermal layer consists of supporting cells, unicellular glands, granular cells and mitochondria-rich cells. The epidermal layer could be classified into superficial, intermediate and basal layer by morphology and structure of the supporting cells. Mucous cells of unicellular gland were observed in the superficial and intermediate layer of the epidermis. The mucous materials were identified as acidic and carboxylated mucosubstance by histochemical methods. Club cell has well-developed central vacuole, rough endoplasmic reticula and Golgi complex in the cytoplasm. Granular cells were observed in the superficial layer and contained numerous granules of high electron density. Mitochondria-rich cells are characterized by well-developed microfilaments in cortex and numerous tubular mitochondria in medullar cytoplasm. Three types of pigment cells in the dermal layer could be distinguished with electron density of cytoplasmic inclusions.

• Korean Journal of Veterinary Research
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• v.37 no.4
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• pp.707-718
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• 1997

The present study was designed to investigate the morphological developments of the stomach in the prenatal and postnatal rats. The gastric fundus of 16- to 22-day-old fetuses, neonates, 7-day-old, 14-day-old, 21-day-old, and adult rats were observed by transmission electron microscopy. 1. In the 19-day-old fetuses, the gastric pits and the parietal cells were the primitive state, but the parietal cells contained numerous microvilli and mitochondria. 2. In the 20-day-old fetuses, the intracellular canaliculus appeared in the parietal cells, and glycogen granules were localized mainly in the basal part of the epithelial cells and parietal cells. 3. In the 20-day-old fetuses, the mucous granules and zonular occludens appeared in the gastric glandular cells adjacent to the parietal cells. 4. In the 21-day-old fetuses, the chief cells were identified in the lamina propria and these cells contained granular endoplasmic reticulum and zymogen granules. 5. In the 20- to 22-day-old fetuses, the definitive parietal cells were firstly appeared in pairs or groups within the lamina propria. These cells tended to be placed near the basement membrane and blood capillary, and frequently tended to be extruded into lumen of gland. 6. The endocrine cells were appeared at the basal areas of the gastric gland after the 14-day-old.

• The Korean Journal of Cytopathology
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• v.14 no.2
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• pp.86-90
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• 2003

Kimura's disease is a chronic inflammatory disorder of unknown cause and is most prevalent among Asians. The cytologic findings of Kimura's disease are significant numbers of eosinophils in a background of lymphoid cells, occasional fragments of collagenous tissue, proliferation oi vessels, and Warthin-Finkeldey polykaryocytes. Among these features, the most important cytologic feature of Kimura's disease is a significant numbers of eosinophils. We experienced a case of Kimura's disease in the parotid gland which we fatted to recognize on cytology due to the apparent paucity of eosinophils. On careful retrograde reviewing of the cytologic findings, a few scattered leukocytes, previously interpreted as polymorphous leukocytes, had bilobed nuclei and coarse green but granular cytoplasm on Papanicolaou preparation. These leukocytes showed obvious orange-red intracyloplasmic granules as in eosionophils on Giemsa stain. The paucity of eosinophils may be due to the thick fibrosis around lymphoid follicles or any technical error during aspiration. Whereas the Warthin-Finkeldey type giant cell is not a sensitive cytologic marker of Kimura's disease, it may be a helpful cytologic feature. To reach a correct cytologic diagnosis of Kimura's disease, It is important to keep in mind that searching for Warthin-Finkeldey type giant cells and evaluation of Giemsa stain for detection of eosinophils would be helpful.

• The Korean Journal of Cytopathology
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• v.18 no.2
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• pp.165-169
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• 2007

The authors present the fine needle aspiration cytology (FNAC) cytologic findings of a case of extranodal marginal zone B cell lymphoma (MZBCL), which featured abundant plasma cells and eosinophilic histiocytes arising in both parotid glands. A 49-year-old female presented with palpable masses in both parotid glands. She had been suffering from systemic lupus erythematosus and rheumatoid arthritis. The lesions were evaluated by FNAC and smears showed a small number of clusters of oncocytic cells with abundant eosinophilic granular cytoplasm and small nuclei, intermixed with small to medium-sized lymphoid cells containing round to lobulated nuclei, which suggested Warthin's tumor. Some of lymphoid cells had a plasmacytoid appearance, and some scattered large cells contained a large amount of eosinophilic cytoplasm. Bilateral superficial parotidectomy was performed and a histopathologic study indicated MZBCL with abundant plasma cells, intermixed with eosinophilic histiocytes. The presence of oncocytic cells and a mixture of lymphoid and plasma cells indicates Warthin's tumor, but the cytologic features of a relatively monotonous small to medium-sized lymphoid infiltrate suggest the possibility of MZBCL in the clinical setting of an FNAC study performed on a patient suffering from a connective tissue disease.