• BMB Reports
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• 제40권1호
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• pp.1-6
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• 2007

Apoptosis signal-regulating kinase 1 (ASK1) is a mitogenactivated protein kinase (MAPK) kinase kinase that activates JNK and p38 kinases. ASK1 is activated by various stresses, such as reactive oxygen species (ROS), endoplasmic reticulum (ER) stress, lipopolysaccharide (LPS) and calcium influx which are thought to be responsible for the pathogenesis or exacerbations of various human diseases. Recent studies revealed the involvement of ASK1 in ROS- or ER stressrelated diseases, suggesting that ASK1 may be a potential therapeutic target of various human diseases. In this review, we focus on the current findings for the relationship between pathogenesis and ASK1-MAPK pathways.

• Journal of Microbiology and Biotechnology
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• 제26권8호
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• pp.1439-1445
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• 2016

A yellow, rod-shaped, non-motile, gram-negative, and strictly aerobic bacterial strain, designated LPB0005^T, was isolated from a marine gastropod, Reichia luteostoma. Here the genome sequence was determined, which comprised 3,395,737 bp with 2,962 protein-coding genes. The DNA G+C content was 36.3 mol%. The 16S rRNA gene sequence analysis indicated that the isolate represents a novel genus and species in the family Flavobacteriaceae, with relatively low sequence similarities to other closely related genera. The isolate showed chemotaxonomic properties within the range reported for the family Flavobacteriaceae, but possesses many physiological and biochemical characteristics that distinguished it from species in the closely related genera Ulvibacter, Jejudonia, and Aureitalea. Based on phylogenetic, phenotypic, and genomic analyses, strain LPB0005^T represents a novel genus and species, for which the name Cochleicola gelatinilyticus gen. nov., sp. nov. is proposed. The type strain is LPB0005^T (= KACC 18693^T = JCM 31218^T).

• Journal of Microbiology and Biotechnology
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• 제21권11호
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• pp.1166-1173
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• 2011

Meju is a traditional Korean fermented soy product used as a key element for soy sauce and doenjang. Bacilli with antimicrobial activity were isolated from meju prepared by traditional methods at Sunchang county, Jeollabukdo, Korea. Six isolates were identified as Bacillus amyloliquefaciens by recA gene sequencing and RAPD-PCR. One isolate, B. amyloliquefaciens MJ5-41, showed the strongest fibrinolytic activity. A 27 kDa active fibrinolytic enzyme, AprE5-41, was purified from the culture supernatant of MJ5-41 grown on LB by chromatographic methods. The optimum pH and temperature for purified AprE5-41 were 7.0 and $45^{\circ}C$, respectively. AprE5-41 quickly degraded $A{\alpha}$ and $B{\beta}$ chains but not the ${\gamma}$-chain of fibrinogen. AprE5-41 exhibited the highest specificity for N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide, a known substrate for ${\alpha}$-chymotrypsin, cathepsin G, and subtilisin BPN'. The structural gene, aprE5-41, was cloned by PCR and successfully expressed in B. subtilis.

• Journal of Information Processing Systems
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• 제16권3호
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• pp.699-717
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• 2020

Propagation time on permissionless blockchain plays a significant role in terms of stability and performance in the decentralized systems. A large number of activities are disseminated to the whole nodes in the decentralized peer-to-peer network, thus causing propagation delay. The stability of the system is our concern in the first place. The propagation delay opens up opportunities for attackers to apply their protocol. Either by accelerating or decelerating the propagation time directly without proper calculation, it brings numerous negative impacts to the entire blockchain system. In this paper, we thoroughly review and elaborate on several parameters related to the propagation time in such a system. We describe our findings in terms of data communication, transaction propagation, and the possibility of an interference attack that caused an extra propagation time. Furthermore, we present the influence of block size, consensus, and blockchain scalability, including the relation of parameters. In the last session, we remark several points associated with the propagation time and use cases to avoid dilemmas in the light of the experiments and literary works.

• Molecules and Cells
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• 제43권1호
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• pp.76-85
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• 2020

MARCH5 is a RING finger E3 ligase involved in mitochondrial integrity, cellular protein homeostasis, and the regulation of mitochondrial fusion and fission. To determine the function of MARCH5 during development, we assessed transcript expression in zebrafish embryos. We found that march5 transcripts were of maternal origin and evenly distributed at the 1-cell stage, except for the mid-blastula transition, with expression predominantly in the developing central nervous system at later stages of embryogenesis. Overexpression of march5 impaired convergent extension movement during gastrulation, resulting in reduced patterning along the dorsoventral axis and alterations in the ventral cell types. Overexpression and knockdown of march5 disrupted the organization of the developing telencephalon and diencephalon. Lastly, we found that the transcription of march5 was tightly regulated by the transcriptional regulators CHOP, C/EBPα, Staf, Znf143a, and Znf76. These results demonstrate the essential role of March5 in the development of zebrafish embryos.

• 유통과학연구
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• 제20권9호
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• pp.61-71
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• 2022

Purpose: This study analyzes the effects of variables affecting e-satisfaction and e-loyalty in e-commerce. Research design, data, and methodology: A survey was conducted to 384 Indonesia online shopping mobile application users. The questionnaire construct was designed based on several independent variables, such as application attractiveness, application functionality, and customer service on e-satisfaction and e-loyalty. E-satisfaction was positioned as an intervening variable to determine the effect on e-loyalty. Results: Application attractiveness, application functionality, and customer service shown positive effect on e-satisfaction in online shopping mobile application. At the same time, e-satisfaction also affected e-loyalty significantly as referred in previous studies. However, application attractiveness, while showed positive effect on e-satisfaction, in contrary with previous study does not showed very significant effect. Conclusion: Application functionality significantly influences users satisfaction compared to application attractiveness. E-satisfaction mediates as a factor between visual design, information access, and transaction in online shopping mobile application, which stimulates user loyalty. The study findings' can be a reference for companies to create and maintain users' satisfaction and loyalty via different aspects of mobile application. Further research should be conducted on other field of study as the industry has different aspects that affects user loyalty.

• Advances in materials Research
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• 제12권2호
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• pp.119-131
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• 2023

The development of sustainable composites materials, from recycled polymeric materials and waste from the wood industry and stone processing, allows reducing the volume of these by-products, minimizing impacts on health and the environment. Nowadays, Polypropylene (PP) is the most recycled polymer in industry, while the furniture industry has increasingly used timber felled from sustainable forest plantations as a eucalypt. The powder tailing from the ornamental stone extraction and processing industry is commonly disposed of in the environment without previous treatment. Thus, the technological option for the development of composite materials presents itself as a sustainable alternative for processing and manufacturing industries, enabling the development of new materials with special technical features. The results showed that powder granite particles may be incorporated into the polypropylene matrix associated with short eucalyptus fibres forming green hybrid composites with potential application in structural engineering, such as transport and civil construction industries.

• Restorative Dentistry and Endodontics
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• 제46권3호
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• pp.37.1-37.11
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• 2021

Objectives: This systematic review evaluated the efficacy of the supplementary use of the XP-endo Finisher on bacteria content reduction in the root canal system. Materials and Methods: In-vitro studies evaluating the use of the XP-endo Finisher on bacteria content were searched in four databases in July 2020. Two authors independently screened the studies for eligibility. Data were extracted, and risk of bias was assessed. Data were meta-analyzed by using random-effects model to compare the effect of the supplementary use (experimental) or not (control) of the XP-endo Finisher on bacteria counting reduction, and results from different endodontic protocols were combined. Four studies met the inclusion criteria while 1 study was excluded from the meta-analysis due to its high risk of bias and outlier data. The 3 studies that made it to the meta-analysis had an unclear risk of bias for at least one criterion. Results: No heterogeneity was observed among the results of the studies included in the meta-analysis. The study excluded from the meta-analysis assessing the bacteria counting deep in the dentin demonstrated further bacteria reduction upon the use of the XP-endo Finisher. Conclusions: This systematic review found no evidence supporting the supplementary use of the XP-endo Finisher on further bacteria counting the reduction in the root canal.

• 한국정보과학회 언어공학연구회:학술대회논문집(한글 및 한국어 정보처리)
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• 한국정보과학회언어공학연구회 2020년도 제32회 한글 및 한국어 정보처리 학술대회
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• pp.76-81
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• 2020

법률 전문 지식이 없는 사람들이 법률 정보 검색을 성공적으로 하기 위해서는 일반 용어를 검색하더라도 전문 용어가 사용된 법령정보가 검색되어야 한다. 하지만 현 판례 검색 시스템은 사용자 선호도 검색이 불가능하며, 일반 용어를 사용하여 검색하면 사용자가 원하는 전문 자료를 도출하는 데 어려움이 있다. 이에 본 논문에서는 일반용어가 사용된 질의문과 전문용어가 사용된 판례를 자동으로 연결해 주고자 하였다. 질의문과 연관된 판례를 자동으로 연결해 주기 위해 전문용어가 사용된 전문가 답변을 바탕으로 문서분류에 높은 성능을 보이는 Doc2Vec을 이용한다. Doc2Vec 문서 임베딩 기법을 이용하여 전문용어가 사용된 전문가 답변과 유사한 답변을 제안하여 비슷한 주제의 답변들끼리 분류하였다. 또한 전문가 답변과 유사도가 높은 판례를 제안하여 질의문에 해당하는 판례를 자동으로 연결하였다.

• 한국정보과학회 언어공학연구회:학술대회논문집(한글 및 한국어 정보처리)
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• 한국정보과학회언어공학연구회 2020년도 제32회 한글 및 한국어 정보처리 학술대회
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• pp.548-553
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• 2020

우리가 쓰는 일상 언어 중에는 언어적 직관이 없는 사람은 의미 파악이 힘든 관용표현이 존재한다. 관용표현을 이해하기 위해서는 표현에 대한 형태적, 의미적 이해가 수반되어야 하기 때문이다. 기계도 마찬가지로 언어적 직관이 없기 때문에 관용표현에 대한 자연어 처리에는 어려움이 따른다. 특히 일반표현과 중의성 관계에 있는 관용표현의 특성이 고려되지 않은 채 문자적으로만 분석될 위험성이 높다. 본 연구에서는 '관용표현은 주변 문맥과의 관련성이 떨어진다'라는 가정을 중심으로 워드 임베딩을 활용한 관용표현과 일반표현에 대한 구분을 시도하였다. 실험은 4개 표현에 대해 이루어 졌으며 Skip-gram, Fasttext를 활용한 방법을 통해 관용표현은 주변 단어들과의 유사성이 떨어짐을 확인하였다.