• KSBB Journal
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• v.17 no.1
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• pp.88-92
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• 2002

The election profiles with different flow rates of motile phase were experimentally measured to investigate the effects on HETP in capillary gas chromatography. Based on the correlation coefficient ($r^2$), the first and second moments as well as HETP were determined from the elution cuties, and the parameters used in empirical equations were estimated. The empirical equations were expressed in terms of mobile phase velocity, and the theoretical Golay equation was considered. The samples were d-limonene and perillyl alcohol, potential anticancer agent mainly was contained in the peel of orange. From the experimental results, HETP of the samples were increased with mobile phase velocities. The correlation coefficient of d-limonene was in the range between 0.8265 and 0.8465, while that of perillyl alcohol was between 0.9353 and 0.9374. The total HETP was composed of $H_{g}$, $H_{l}$ and Hc, and Hg had the greatest effect on HETP, but HI showed a negligible effect. In terms of HETP, the optimum velocity of mobile phase was present.