• 한국식품영양과학회지
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• 제23권3호
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• pp.429-435
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• 1994

자라나는 과정의 젊은 흰쥐와 14개월 된 늙은 흰쥐, 생쥐들을 대상으로 Met 투여에 따른 간, 혈장, 신장, 비장 등의 glutathione peroxidase(GSHPx) 효소 변화를 측정 비교하였다. 성장기의 쥐에 있어서 Se을 Selenite 형태로 투여했을 때 0.5%의 식이 Met 공급은 같은 Se을 Se-Met 형태를 공급했을 때 보다 효소 활성이 증가하였다. 그러나, 14개월 된 늙은 쥐에서는 Met 투여에 대한 효소 활성은 큰 변화가 없었다. 일반적으로 식이 Se 증가는 효소 활성과 거의 정비례하나 1ppm Se 수준 이상에서는 완만하게 증가 또는 거의 정체하는 상태를 보이지만, 늙은 쥐에서는 Se의 식이 수준이 2ppm이 되어도 여전히 효소 활성이 증가하는 경향을 보였다. 성장기의 생쥐 실험에서도 2ppm Se 수준에서의0.3%와 0.8%의 식이 Met 투여는 효소 활성에 큰 영향이 없었다. 그러나 실험식이 투여 18일 경엔 4일째 보다 Met 투여군이 4.2배의 증가를 보인 반면 비투여군은 2.5배의 증가에 머물렀다. 18일경 이후 효소 활성이 감소하여 42일재는 반으로 감소함을 보였다. Se의 생체 활성은 특히 식이 Se 수준이 낮을 때 Met에 의해 증가되는 폭이 컸으며 Se 수준이 높을수록 상대적으로 감소함을 보였다. 위의 제반 결과에서 Met의 요구량이 Se-Met에 의해 대체되고 있음을 짐작할 수 있다. 그러므로 식이 Met 수준 상태에 따라 효소의 활성이 달라질 수 있다는 것을 시사한다.

• Journal of Nutrition and Health
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• 제27권9호
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• pp.940-948
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• 1994

The present study was designed to determine if prenatal and postnatal Se nutriture affects Se concentration, glutathione peroxidase(GSHPx) activity and phospholipid distribution of the neonatal rat lung. Female SD rats were bred and fed a semipurified Se-deficient(0.04ppm, Se-) or a Se-adequate(0.5ppm, Se+) diet through pregnancy and lactation. On d 2 of lactation, maternal dietary Se had no significant effect on pulmonary Se concentration of pups. On d 16 of lactation, mean milk Se concentration in Se- dams was significantly lower than that in Se+ dams. Milk Se concentration was reflected on lung Se concentration and GSHPx activity of d 16 pups, which were dramatically decreased in Se- pups. In addition, pulmonary disaturated phosphatidyl choline/total phosphatidyl choline ratio was also significantly decreased in Se- pups, implying impaired function of pulmonary surfactant. These data indicate that adequate Se nutrition is important in the maturation of neonatal rat lungs.

• 대한수의학회지
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• 제39권4호
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• pp.679-688
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• 1999

The combined effects of iron and selenium status on glutathione peroxidase (GSHPx) activity, cytochrome P-450 activity, and lipid peroxidation in the liver and intestinal mucosa of rats were investigated. In experiment one, four experimental groups (+Se+Fe, -Se+Fe, +Se++Fe, -Se++Fe) were manipulated for 3 weeks with intramuscular administration of irondextran (++Fe) and/or normal diet (+Fe) and deionized water (-Se) and/or selenium-supplemented deionized water (+Se). In experiment two, 2% dietary carbonyl iron (instead of the parenteral administration) was fed for 3 weeks to rats. Body weight of rats was significantly decreased in both parenterally and orally iron-overloaded groups (p<0.01), regardless of Se supplement. Serum iron was significantly increased in parenterally iron-overloaded groups but it was marginally increased in orally iron-overloaded groups. There was no significant difference in hemoglobin content among experimental groups in either experiment one or two. Total iron in the small intestine, intestinal mucosa, and livers was significantly high in both parenterally and orally iron-overloaded rats, regardless of selenium status. In the liver and intestine, GSHPx activity was significantly higher in all selenium-supplemented groups, compared to Se-deficient groups (p<0.01) and lipid peroxidation was significantly enhanced in both parenterally and orally iron-overloaded groups, compared to iron-adequate groups. There was no significant difference in cytochrome P-450 activity in the livers between groups in both experiment one and two. These results indicated that GSHPx activity in liver and intestinal mucosa was depended on selenium status, regardless of iron status, and iron-overload enhances lipid peroxidation in liver and intestinal mucosa by increasing the tissue iron content.

• 생명과학회지
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• 제7권1호
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• pp.10-16
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• 1997

해양오염의 진단을 위한 생화학적 오염지표 설정의 기초 연구의 일환으로, 오염이 심각한 서해(또는 황해)산 넙치(Paralichthys olivaceus)의 혈액 및 뇌중의 활성산소종 및 그들의 제거효소의 활성을 분석 평가하였다. 이들 넙치의 혈청중의 단백질의 함량은 해역이나 양식산의 자연산사이에는 뚜렷한 차이를 발견할 수 없었지만, 서해안 양식산 넙치의 뇌 및 근육중의 단백질 함량이 동해안의 포항의 자연산 넙치(대조군)의 단백질 함량에 비해 각각 30-45%(brain) 및 25-45%(muscle)나 유의적으로 감소하였다(p<0.001). 서해안의 양식 및 자연산 넙치의 혈청중의 과산화지질(MDA)의 량은 대조굼으로 사용한 동해안 포항의 자연산 넙치 대비 각각 30-80% 및 125-145%나 현저히 높았다. 서해안의 양식산 넙치의 혈청중의 히드록시 라디칼의 생성량은 동해안 포항의 자연산 넙치 대비 각각15-30%정도나 오히려 낮았을 뿐만 아니라 서해안의 자연산 넙치도 격포를 제외하고 영광의 자연산 넙치의 히 시 라디칼의 생설양도 약 20%나 낮았다. 서해안의 양식산 넙치의 혈청중의 SOD의 활성은 동해안 포항의 자연산 넙치 대비 15-35%정도나 오히려 낮았을 뿐만 아니라 서해안의 자연산 넙치도 영광은 35%나 낮았지만, 단지 격포만이 SOD의 활성이 35% 정도 높았을 뿐이다. 서해안의 양식산 넙치의 혈청중의 GSHPx의 활성은 동해안 포항의 자연산 넙치 대비 15-25%정도나 유의적으로 높았지만, 서해안의 자연산 넙치의 GSGPx의 활성은 어느 곳이나 낮았다. 이상의 실험결과에서 볼 때 뇌나 근육중의 단백질의 함량이나 뇌중의 GSHPx의 활상, 그리고 혈청중의 과산회지질의 함량이 넙치의 오염지표로서의 가능성을 검토할 가치가 있다고 판단된다.

• Asian-Australasian Journal of Animal Sciences
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• 제23권4호
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• pp.501-507
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• 2010

Three hundred chicks were randomly assigned among four treatments to evaluate the effect of an organic selenium supplement at various levels of dietary protein. Two levels of supplemental selenium (0 and 0.3 mg/kg) from zinc-L-selenomethionine were tested at two levels of dietary protein (normal and reduced) in a completely randomized design with $2{\times}2$ factorial layout. The experiment lasted up to 49 d of age during which all birds were exposed to $31{\pm}1^{\circ}C$. The effects of selenium or its interaction with CP on growth performance and carcass characteristics were not significant. However, feeding the reduced-CP diet decreased weight gain in the starting period and increased liver and abdominal fat weights relative to body weight. Ferric reducing ability of plasma (FRAP) was not significantly affected by dietary CP and Se or their interaction though FRAP values were numerically higher in the Se-supplemented group. Dietary CP content did not affect the activity of plasma glutathione peroxidase (GSHPx), though Se significantly elevated plasma GSHPx activity. The interaction of CP and Se was not significant for FRAP and plasma GSHPx activity.

• 생명과학회지
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• 제9권4호
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• pp.466-472
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• 1999

These studies were designed to investigate the effects of pine (Pinus densiflora Sieb et Zucc.) needle extract (PNE) on oxygen radicals and their scavenger enzymes in liver membranes of Sprague-Dawley (SD) rats as a study on investigation of anti-aging effect and determination of chemical structures of PNE through the animal experiments. Male SD rats were fed basic diets (control group) and experimental diets (0.5% and 1.0%-PNE group) for 6 weeks. There were no significant differences in hydroxyl radical (·OH) formations of liver mitochondria and microsomes in 0.5%-PNE group, while ·OH formations were significantly decreased (10% and 18%, respectively) in liver mitochondria and microsomes of 1.0%-PNE group compared with control group. Microsomal hydrogen peroxides and cytosolic superoxide radicals were remarkably decreased (20% and 20∼25%, respectively) in 0.5% and 1.0%-PNE groups compared with control group. Mn-SOD activities in mitochondria were significantly increased about 10% in 1.0%-PNE group, while Mn-SOD activities in mocrosomes were remarkably increased (16∼20%) in 0.5% and 1.0%-PNE groups compared with control group. There were no significant differences in Cu, Zn-SOD activities of liver cytosol in 0.5% and 1.0%-PNE groups, while glutathione peroxidase (GSHPx) and catalase (CAT) activities were significantly decreased (28∼30% and 15∼30%, respectively) in liver cytosols of 0.5% and 1.0%-PNE groups compared with control group. These results suggest that these PNE may play a effective role in a attenuating a oxygen radical formations and increasing a scavenger enzyme activities.

• 생명과학회지
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• 제8권1호
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• pp.91-96
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• 1998

Pine(Pinus densiflora Sieb et Zucc.) is one of rhe popular plant drugs which has been used as a medicine in Asia. To investigate the effect of pine needle extract (PNE) on oxygen radicals and their scavenger enzymes in brain membranes of Sprague- Dawley (SD), make SD rats were fed basic diets(control group), and experimental diets (PNE group) with 0.5 and 1.0% of PNE 6 weeks. Mitochondrial hydroxyl radical levels in brain of 0.5%-PNE and 1.0%-PNE groups were significantly inhibited to 30% and 25%, respectively, and microsomal hydrogen peroxide levels in brain of 0.5%-PNE and 1.0%-PNE groups were significantly inhibited to 15% compared with control group. Cytosolic superoxide rdical levels in 1.0%-PNE group were significantly inhibited to 20% compared with control group. Lipid peroxide(LPO) levels in brain mitochondria of 0.5%-PNE and 1.0%-PNE groups were significantly lower(25% and 35%) than that in control group. Mn-superoxide disumtase (SOD) activities in brain of 0.5%-PNE and 1.0%-PNE groups were significantly higher(18% and 12%) than those in control groups, but Cu,Zn-SOD activities in brain of 0.5%-PNE were significantly activated to 15% compared with control group. Glutathione peroxidase(GSHPx) activities in brain of 1.5%-PNE and 1.0% PNE groups were significantly higher(14% and 12%) than those in control group. These results suggest that more beneficial effects such as inhibition of oxygen radicals and lipid peroxide(LPO). and oncreases of scavenger enzymes in brain membranes of SD rats may be effectively modulated by administration of pine needle extract (PNE)

• BMB Reports
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• 제39권6호
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• pp.656-661
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• 2006

Cisplatin (CDDP) is a widely used anticancer drug, but at high dose, it can produce undesirable side effects such as hepatotoxicity. Because silymrin has been used to treat liver disorders, the protective effect of silymarin on CDDP -induced hepatotoxicity was evaluated in rats. Hepatotoxicity was determined by changes in serum alanine aminotransferase [ALT] and aspartate aminotransferase [AST], nitric oxide [NO] levels, albumin and calcium levels, and superoxide dismutase [SOD], glutathione peroxidase [GSHPx] activities, glutathione content, malondialdehyde [MDA] and nitric oxide [NO] levels in liver tissue of rats. Male albino rats were divided into four groups, 10 rats in each. In the control group, rats were injected i.p. with 0.2 ml of propylene glycol in saline 75/25 (v/v) for 5 consecutive days [Silymarin was dissolved in 0.2 ml of propylene glycol in saline 75/25 v/v]. The second group were injected with CDDP (7.5 mg /kg, I.P.), whereas animals in the third group were i.p. injected with silymarin at a dose of 100 mg/kg/day for 5 consecutive days. The Fourth group received a daily i.p. injection of silymarin (100 mg/kg/day for 5 days) 1 hr before a single i.p. injection of CDDP (7.5 mg/kg). CDDP hepatotoxicity was manifested biochemically by an increase in serum ALT and AST, elevation of MDA and NO in liver tissues as well as a decrease in GSH and the activities of antioxidant enzymes, including SOD, GSHPx in liver tissues. In addition, marked decrease in serum NO, albumin and calcium levels were observed. Serum ALT, AST, liver NO level, MDA was found to decreased in the combination group in comparison with the CDDP group. The activities of SOD, GSHPx, GSH and serum NO were lower in CDDP group than both the control and CDDP pretreated with silymarin groups. The results obtained suggested that silymarin significantly attenuated the hepatotoxicity as an indirect target of CDDP in an animal model of CDDP-induced nephrotoxicity.

• 한국잠사곤충학회지
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• 제41권3호
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• pp.216-221
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• 1999

This study was designed to investigate the effects of silk fibroin powder on oxygen radicals and their scavenger enzymes in serum of rats. Sprague-Dawley(SD) male rats (160${\pm}$10 g) were fed experimental diets (SFP-2.5 and SFP-5.0 groups)added 2.5 and 5.0 g/kg BW/day for 6 weeks. Triglyceride levels were remarkably inhibited (15∼25%) in SFP-2.5 and SFP-5.0 groups compared with control group. Hydroxyl radical ($.$OH) formations resulted in a marked decrease (15%∼25%) compared with control group, while superoxide radical and hydrogen peroxide formations resulted in a significant decrease (6∼12% and 10%). Lipid peroxide and oxidized protein (>C=O group) productions resulted in a significant decrease (6∼12% and 6%) compared with control group. Superoxide dismutase (SOD) and catalase (CAT) activities were remarkably increased (35∼55% and 40∼50%), but glutathione peroxidase (GSHPX) activities were significantly increased (13%) compared with control group. These results suggest that anti-aging effect of silk fibroin powder (SFP) may play a pivotal role in attenuating a various age-related changes.

• 한국잠사곤충학회지
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• 제41권3호
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• pp.141-146
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• 1999

This study was designed to investigate the effects of silkworm powder (SWP) on oxygen radicals and their scavenger enzymes in serum of rats. Sprague Dawley (SD) male rats (160${\pm}$10g) were fed experimental diets (SWP-200and SWP-400 groups)added 200 and 400mg/kg BW/day for 6 weeks. Triglyceride (TG)levels were significantly inhibited (10% and 25%) in SWP-200 and SWP-400 groups, but there were no singificant differences in total, LDL-and HDL-cholesterol levels in both SWP-200 and SWP-400 groups. Hydroxyl radical ($.$OH) formations resulted in a marked decreases (about 20%) in both SWP-200 and SWP-400 groups compared with control group. Superoxide radical (O2.-)and hydrogen peroxide formations resulted in a significant decreases in SWP-400 group compared with control group. Lipid peroxide (LPO)and oxidized protein(>C=O group) productions resulted in a significant decreases (SOD) and catalase (CAT) activiteies were remarkably increased (10∼25% and 40∼50%)in SWP-200 and SWP-400groups. Glutathione peroxidase(GSHPX) activities were significantly increased (about 10%) in SWP-400 groups compared with control group. These results suggest that anti-aging effect of silkworm powder (SWP) may play a pivotal role in attenuation a various chronic degenerative diseases age-related changes.