• Journal of Korean Neurosurgical Society
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• 제37권1호
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• pp.44-47
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• 2005

Objective: The purpose of this study is to investigate the elevated blood glucose levels in the postoperative period are associated with an increased risk of deep wound infection in diabetic individuals undergoing lumbar spine surgery. Methods: Of 2896 patients who underwent lumbar spine operations by one surgeon between 1993 and 2002, 329(11.4%) were diabetics. The rate of deep wound infections in diabetic patients was 6.4%, versus 3.2% for nondiabetics. 152 patients had their operation before implementation of the protocol and 177 after implementation. Charts of the diabetic patients were reviewed. Mean blood glucose levels were calculated from documented results of finger-stick glucometer testing. Results: Twenty-one diabetic patients suffered deep wound infection. Infected diabetic patients had a higher mean blood glucose level through the first 2 postoperative days than noninfected patients($230{\pm}6.9$ versus $175{\pm}3.8mg/dL$; p<0.003) and had a long operation time($216{\pm}57.9$ versus $167.5{\pm}42.2$ minute; p<0.05). Multivariable logistic regression showed that mean blood glucose level for the first 2 postoperative days, long operation time, and use of the instrumentation(p<0.02) were all related predictiors of deep wound infection. Institution of a protocol of postoperative continuous intravenous insulin to maintain blood glucose level less than 200mg/dL was began in september 1997. This protocol resulted in a decrease in blood glucose levels for the first 2 postoperative days and a concomitant decrease in the proportion of patients with deep wound infection, from 8.3%(11/132) to 5.1%(10/195) (p<0.02). Conclusion: The incidence of deep wound infection in diabetic patients is reduced after implementation of a protocol to maintain mean blood glucose level less than 200mg/dL in the immediate postoperative period.

• 대한임베디드공학회논문지
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• 제15권4호
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• pp.167-175
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• 2020

A blood-glucose meter is one of the in vitro diagnostic devices to measure and control the glucose concentration of diabetics. In order to measure the glucose level in the blood, the common method is to measure the amount of electrons, that is, the output current generated by glucose oxidation after a blood sample is inserted into the test strip containing an enzyme. The hematocrit is an obstacle in measuring accurate blood glucose concentration. This paper deals with the design and implementation of a blood-glucose meter to correct the hematocrit interference. We propose a sequential method which measures impedance using the alternating current and then measures glucose in the blood using the direct current. In addition, this paper introduces how to use commercial glucose strips based on the proposed system. Finally, we conducted the performance evaluation of the proposed system by comparing the measured current and impedance with those of the references. As a result, the standard deviation of the current measurement is approximately 0.6nA and the impedance measurement error for measuring the hematocrit is approximately within 1%. The proposed system will improve the accuracy of the conventional blood-glucose meter by reducing the hematocrit interference.

• The Korean Journal of Physiology
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• 제21권2호
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• pp.323-325
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• 1987

The concentrations of glucose and glycogen in the normal gastrocnemius muscles of Uromastix hardwickii were $88.82{\pm}4.52\;mg/100\;gm$ and $158.98{\pm}23.19\;mg/100gm$ of wet weight of the muscle, respectively. 14-days denervation period has no any effect on glucose contents while the glycogen concentration was decreased to 1/3 of the normal control innervated muscles.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 춘계학술발표대회
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• pp.45-50
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• 2000

A. pullulan ATCC 42023를 사용하여 고분자량의 pullulan을 대량생산하기 위한 연구로써, 탄소원의 농도, 용존산소량과 pH가 균체 성장 및 pullulan 생산에 미치는 영향에 대하여 연구하였다. Glucose의 당 농도에 따른 실험결과에서 glucose 농도가 0.3M (54g/1)일 때 최대의 pullulan 생산률을 나타내었고, 0.3M (54g/1)이상에서는 productivity가 급격하게 감소하는 기질 저해현상을 나타내었다. 삼각플라스크를 이용한 초기 pH의 영향에 대한 실험결과, 초기 pH 6.5는 $11.98g/{\ell}$의 pullulan을 생산하였고, pH 4.5로 일정하게 조절한 결과, pullulan 생산은 $13.31g/{\ell}$의 최대값을 나타내었다. 그러나 균체성장은 pH 6.5에서 가장 높았다. 그리고 용존산소량의 증가로 pullulan productivity를 증가시킬 수 있었다. 또한 산업적으로 유용한 고분자량의 pullulan을 생산하기 위한 탄소원으로 아미노당인 glucosamine을 사용하여 pullulan의 생성 및 분자구조 변형에 대하여 실험하였다. 탄소원 농도 50g/1 기준에서, glucose와 glucosamine을 40 : 60의 비율로 혼합하여 공급 하였을 때 분자량 20만 이상의 mediun size 및 200만 이상의 high molecular weight의 productivity가 가장 높았으며, A. pullulans의 specific growth rate도 가장 높았다.

• Journal of Nutrition and Health
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• 제26권7호
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• pp.851-857
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• 1993

Atherosclerotic vascular disease is a major cause of the increased morbidity and mortality assciated with diabetes mellitus. The prominent role of nutrition in hypercholesteolemia and atherosclerosis is generally accepted. Diet is a key element in the management of diabetes (type I-IDDM), yet the appropriate diet for patient with diabetes mellitus is not well known. Dietary protein has been shown to have a significant effect on plasma cholesterol levels in both experimental animals and humans. The present experiment was designed to determine the effect of the dietary protein level(20% vs 60%) on plasma glucose concentration, lipids profile, insulin and glucagon levels from non-diabetic and streptozotocin-induced diabetic rats. Results showed that a high protein diet decreased triglyceride concentration in diabetic rats. Also diabetic rats fed a high protein diet were hypocholesterolemic than rats fed a control diet. There were no effects by level of protein on fasting blood glucose concentration and insulin/glucagon ratio. Results from the present study suggest that a high protein diet may be beneficial to control pasma lipids in chemically-induced diabetic rats.

• 한국응용과학기술학회지
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• 제25권3호
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• pp.347-354
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• 2008

This study was done to investigate the antidiabetic effect of polygoni radix in Streptozotocin(STZ)-induced diabetic rats. Diabetes was induced by intravenous injection of STZ at a dose of 45mg/kg dissolved in citrate buffer. The ethanol extract of polygoni radix was orally administrated once a day for 7 days. The content of serum glucose, triglyceride(TG), total cholesterol were significantly decreased in polygoni radix treated STZ-sample group compared to the those of STZ-control group. The content of hepatic glycogen and activities of glucose-6-phosphate dehydrogenase(Glucose-6-PDH), glucokinase were significantly increased, but activity of glucose-6-phoshatase was decreased in polygoni radix treated STZ-sample group compared to the those of STZ-control group. These results indicated that ethanol extract of polygoni radix would have antidiabetic effect in STZ-induced diabetic rats.

• Clinical and Experimental Reproductive Medicine
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• 제27권1호
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• pp.1-7
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• 2000

Objective: Mammalian embryos undergo changes of energy environment for transfer from oviduct to uterus. Also, the human reproductive organ (oviduct, uterus) contains energy sources of different concentration (oviduct - glucose: 0.5 mM, pyruvate: 0.32 mM, lactate: 10.5 mM; uterus - goucose: 3.15 mM, pyruvate: 0.1mM, lactate: 5.87 mM, respectively). This study was conducted to examine the effect of these energy sources added in DMEM with glutamine on the mouse embryo development. Methods: There was used ICR female mouse. Two cell embryos of mouse are collected by method of 'flushing'. Flushing fluid was used Ham's F-10 added to 20% FBS. The collected 2 cell embryos were cultured in media such as Control (only DMEM), group A and B (DMEM supplemented with 0.5 mM and 3.15 mM glucose), and group C and D (DMEM supplemented with 0.1 mM and 0.32 mM pyruvate), and group E and F (DMEM supplemented with 5.87 mM and 10.5 mM lactate). All experimental media supplemented with 20% hFF, respectively. Pattern of embryo development was observed to interval at 24hr during 96hr. Results : The media with glutamine added glucose (group A: 51.0%; group B: 48.4%) was significantly (p<0.05) higher than other experimental group in development into the morula stage after 24 hr in culture, but not significantly different compared with control and the rate of development into the blastocyst was significantly (p<0.05) low in the both of pyruvate (group C: 7.9% group D: 6.8%) and lactate (group E: 7.1%, group F: 7.1%) treatment group after 48 hr in culture. Development into the blastocyst and hatched balstocyst after 72 hr in culture revealed similarly in control (81.9%) and glucose treatment group (group A: 83.3%, group B: 82.8%). However, development into the hatched and attached blastocyst after 96hr in culture revealed significantly (p<0.05) development in the glucose treatment group (group A: 82.3%, group B: 78.5%) than control (63.2%), and its of pyruvate (group C: 34.1%, group D: 34.1%) and lactate (group E: 25.9%, group F: 33.3%) treatment group were significantly (p<0.05) lower than control similar to previous observations. Conclusion : The glucose added to the DMEM with only glutamine, as energy source, was highly to the rate of development compared with control, but the other energy sources were not, synthetically. Above refer to, the human reproductive organ (oviduct, uterus) contains energy sources of different concentration. Thus, further studies are will examine continuously to effects by interaction of different energy sources in the mouse embryo development, and these results will provide to foundation on the human embryo culture.

• 한국간호교육학회지
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• 제19권2호
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• pp.127-136
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• 2013

Purpose: The aim of the study was to evaluate the effect of self-monitoring of blood glucose (SMBG)-based Diabetes Self-Management Education (DSME) on glycemic control in type 2 diabetes. Methods: This study was designed to compare changes in glycemic control over 12months in SMBG-based DSME group (n=65) versus control group (n=65). Data were obtained from medical records type 2 diabetic patients treated with oral antidiabetic agents and above HbA1c 7.0% from June 2006 to August 2008. All participants completed DSME defined as informational intervention of lifestyle habits and reinforcement of educational Monthly News letter delivered by the diabetes nurse educator. SMBG-based DSME group requested to measure blood glucose 7 times a day for a week and to record their diary and received counseling with a focus on diet and lifestyle during the education. Assessments were conducted baseline, 3, 6 and 12 months. HbA1c was used as an index of glycemic control. Results: 12 months later, the level of HbA1c was reduced by $1.28{\pm}1.68%$ in experimental group and $0.49{\pm}1.05%$ in the control group. We found a significant effect of $Time^*$ Group interaction (p=.013). Conclusion: SMBG-based DSME for patients with type 2 diabetes with oral antidiabetic agents was effective in improving glycemic control and maintaining long-term glycemic control.

• 대한한의학방제학회지
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• 제13권2호
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• pp.41-57
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• 2005

This study has been carried out to understand the effect of Danchisoyosangagambang (DC) on the hyperglycemic mice induced with streptozotocin(STZ). Experimental groups were made diabetic mice by intraperitoneal injection of STZ(60 mg/kg of body weight) tw ice by 24 h interval and then 120 mg/kg STZ was injected again 3 days after the earlier treatment. Control group was administered mice with 0.9 % saline(2 mL/kg), and experim ental groups were administered DC extract(DCA group, 10 mg/kg/day; DCB group, 30 mg/kg/day) after hyperglycemic induction for 6 weeks. The body weight of experimental groups was lower than control. The blood glucose concentration increased continuously, rea ching to 298.9 mg/dL after 6 weeks, however, experimental groups of the DCA and DCB groups significantly(p<0.0l) decreased in the 4, 5, and 6 weeks groups. Blood glucose tolerance test was not significant between control and experimental groups. We examined the blood transaminase activities to know the effect of herbal medicine on liver function. The GOT activities were lower in group DCB than in control. The GPT activities were lower in group DCA and DCB than in control. The content of triglyceride was significantly increased in group DCA compared to control. The SOD and catalase activities were higher in the group DCA compared to control. The results of immunohistochemical study, a few of insulin positive cells observed in the control and experimental group. These results suggest that administration of DC extract to the hyperglycemic mice decreased the blood glucose level.

• 한국식품과학회지
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• 제10권3호
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• pp.350-355
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• 1978

Glucose-ammonia(각 0.2M)와 glucose-glycine(각 0.2M) 갈색화(褐色化) 반응액(反應液)의 색깔을 측정하고, 또한 이들의 ethanol 추출물(抽出物)들이 함유된 대두유(大豆油)의 POV, TBA가(價) 및 산가(酸價)를 정기적으로 측정하여 그항산화작용(抗酸化作用)을 비교하였다. Glucose-ammonia 반응액(反應液)의 2, 5시간후의 absorbance (470 nm)는 각 1.88 및 3.42였으며, 후자(後者)의 경우 0.02와 0.07였다. 반응액(反應後) 2,15 및 40시간이 경과한 glucose-ammonia 및 glucose-glycine 반응액(反應液)에서 얻은 추출물(抽出物)들이 들은 기질(基質)$(42.3{\pm}2.6^{\circ}C)$의 30일간 저장후의 과산화(過酸化) 물가(物價)는 15.8, 14.2, 12.6과 17.4, 16.1, 15.8였으며 control의 경우 82.1였다. TBA가(價)및 산가(酸價)도 비슷한 추세를 보였다. Glucose-ammonia반응액(反應液)의 ethanol추출물(抽出物)의 항산화작용(抗酸化作用)은 glucose-glycine 반응액(反應液)의 경우보다 다소 컸으며, 다같이 반응초기(反應初期)에 이미 항산화물질(抗酸化物質)들이 형성(形成)된듯 했다. 한편, 전자(前者)의 경우, 형성(形成)된 갈색물질(褐色物質)들은 그항산화작용(抗酸化作用)에는 크게 기여(寄與)하지 않는듯 했다.