• Title/Summary/Keyword: Gluconacetobacter hansenii

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Sensory Characteristics of Citrus Vinegar fermented by Gluconacetobacter hanenii CV1 (Gluconacetobacter hansenii CV1에 의해 발효된 감귤식초의 관능적 특성)

  • Kim Mi-Lim;Choi Kyung-Ho
    • Korean journal of food and cookery science
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    • v.21 no.2 s.86
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    • pp.263-269
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    • 2005
  • Citrus juice, a concentrate manufactured by the Jeju Provincial Corporation, was converted into vinegar orderly by alcohol and acetate fermentation. The juice with 6 folds dilution by distilled water was used as the sole nutrient source through out experiments. Diluted juice contained $12.96^{\circ}Brix$ of total sugar, $0.632\%$ of total acid and $20.23{\mu}g/m{\ell}$ of hesperidin. Naringin was not detected from the juice. Citrus wine having $5.6\~6.3\%$ alcohol was produced from diluted juice by 3 days of fermentation at $28^{\circ}C$. A kind of malomelo yeast CMY-28 was used for wine fermentation. The wine was succeedingly fermented for 8 days at $30^{\circ}C$ after inoculation of seed vinegar which contained active cells of acid producing bacteria CV1. Inoculum size of seed vinegar was controlled to $10\%$(v/v) of citrus wine. The wine converted into vinegar by the fermentation. Citrus vinegar, the final product of fermentation, was colored with very thin radish-yellow and transparent. It's acidity ranged between $5.8\~6.2\%$ as acetic acid. The vinegar got the best score by sensory test among several natural fruit vinegars. It was clear from the results that citrus vinegar in high quality could be produced from concentrated citrus juice, however fermentation conditions should be improved to reduce the amount of reducing alcohol.

Preparation and Characterization of Gelatin-immobilized Bacterial Cellulose Scaffold for Tissue Engineering Using Gamma-ray Irradiation (감마선을 이용한 조직공학용 젤라틴이 개질된 미생물 셀룰로오스 지지체의 제작 및 특성)

  • Choi, Jong-Bae;Jeong, Sung In;Gwon, Hui-Jeong;Park, Jong-Seok;Nho, Young-Chang;Choi, Young-Hun;Park, Kyung Jin;Park, Man Yong;Shin, Heungsoo;Lim, Youn-Mook
    • Journal of Radiation Industry
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    • v.6 no.2
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    • pp.159-164
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    • 2012
  • Bacterial cellulose (BC) is generated from citrus gel by Gluconacetobacter hansenii TL-2C. BC has good properties such as high-burst pressure, high-water contact and the ultrafine highly nanofibrous structure of mimic natural extracellular matrix (ECM) for tissue engineering. In this study, acrylic acid (AAc) was grafted onto BC surfaces under aqueous conditions using gamma-ray irradiation, and then immobilized gelatin onto AAc-g-BC. The characterization of scaffolds was performed by scanning electron microscopy (SEM), attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), toluidine blue O (TBO) assay. Morphology of gelatin and AAc incorporation onto BC nanofibers did not changed. Our study suggests that gelatin-immobilized BC nanofibers scaffold has a potentiality to fabricate 3D nanofibrous scaffolds for tissue engineering.